Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,155,982 C→T T23S (ACC→TCT)  RPPX_05120 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,155,9820CT87.6% 1405.6 / 133.6 484T23S (ACC→TCT) RPPX_05120hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/30);  new base T (188/235);  total (219/265)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

Only 100 of 492 total aligned reads displayed.

CCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCCGGGGCGAGATCGATCAGGAAGGTGCCGTTGGCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCAGCGTCACGCTGTTCCCCGCCCAGGCCAATGGTGAAGCACTCGATGTGCGTCTGGTGGATGCCGCCGGCACCGGCTTCACC  >  CP009974/1155836‑1156132
                                                                                                                                                  |                                                                                                                                                      
accgatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCg                                                                                                                                                    <  1:234755‑M1/10‑1 (MQ=255)
  cgatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTg                                                                                                                                                  <  2:405364‑M1/12‑1 (MQ=255)
   gatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGc                                                                                                                                                 >  1:3633138‑M1/139‑151 (MQ=255)
   gatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGc                                                                                                                                                 <  1:6817657‑M1/13‑1 (MQ=255)
    atgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCa                                                                                                                                                <  1:4040225‑M1/14‑1 (MQ=255)
     tgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTaccgtccac                                                                                                                                               <  2:5384315‑M1/15‑5 (MQ=255)
     tgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAg                                                                                                                                               >  2:2995717‑M1/137‑151 (MQ=255)
     tgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAg                                                                                                                                               >  1:2519295‑M1/137‑151 (MQ=255)
       cggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGt                                                                                                                                             >  2:3395758‑M1/135‑151 (MQ=255)
         gcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGc                                                                                                                                           <  2:4746895‑M1/19‑1 (MQ=255)
         gcaggcaacgccgccgccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGc                                                                                                                                           >  2:7080580‑M1/133‑151 (MQ=255)
           aggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGt                                                                                                                                         <  2:4839135‑M1/21‑1 (MQ=255)
            ggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTg                                                                                                                                        >  1:6263457‑M1/130‑151 (MQ=255)
            ggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTg                                                                                                                                        >  2:6257513‑M1/130‑151 (MQ=255)
             gcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGa                                                                                                                                       >  2:5584980‑M1/129‑151 (MQ=255)
              caacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAc                                                                                                                                      >  1:1370723‑M1/128‑151 (MQ=255)
              caacgcctccaccgccaataaccgtaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAc                                                                                                                                      >  1:3858900‑M1/128‑151 (MQ=255)
               aacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcg                                                                                                                                     <  1:6905697‑M1/25‑1 (MQ=255)
                 cgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgcccaatctggtgatcggcgctgccggcagccagctcagcggtcgcggcgaagccGGCTCTACCGTGcacgtgcgcgccgtc                                                                                                                                   <  2:3834316‑M1/27‑8 (MQ=255)
                 cgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcgcg                                                                                                                                   <  2:4654439‑M1/27‑1 (MQ=255)
                 cgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcgcg                                                                                                                                   <  2:1130036‑M1/27‑1 (MQ=255)
                   cctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGtggc                                                                                                                                 <  2:6556909‑M1/29‑1 (MQ=255)
                       caccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGtggcaggc                                                                                                                             >  2:260285‑M1/119‑151 (MQ=255)
                       caccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGcggccggc                                                                                                                             >  1:206006‑M1/119‑151 (MQ=255)
                        accgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCa                                                                                                                            <  2:219955‑M1/34‑1 (MQ=255)
                         ccgccagtagccttgccgcggcgggcagcgacgggggcgacacgacgccaccggaggcgccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCaa                                                                                                                           >  2:6458788‑M1/117‑151 (MQ=255)
                          cgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggtggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCCTGCAAc                                                                                                                          <  1:1797689‑M1/36‑1 (MQ=255)
                          cgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAAc                                                                                                                          <  2:6915919‑M1/36‑1 (MQ=255)
                          cgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAAc                                                                                                                          <  1:4951993‑M1/36‑1 (MQ=255)
                          cgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAAc                                                                                                                          <  1:5289296‑M1/36‑1 (MQ=255)
                            ccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACAt                                                                                                                        <  1:3095688‑M1/38‑1 (MQ=255)
                            ccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACAt                                                                                                                        <  1:2732675‑M1/38‑1 (MQ=255)
                             caatacccctaccgcgccggacagcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATc                                                                                                                       >  2:5231098‑M1/113‑151 (MQ=255)
                              aataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATcc                                                                                                                      >  1:5840267‑M1/112‑151 (MQ=255)
                                taaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTc                                                                                                                    >  1:3357100‑M1/110‑151 (MQ=255)
                                  cccttaccgcgccagacatcgacggcggcgacacaacgccacaggagaccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTcgc                                                                                                                  >  1:1042436‑M1/108‑151 (MQ=255)
                                  accttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTcgc                                                                                                                  >  1:2868225‑M1/108‑151 (MQ=255)
                                     ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGAc                                                                                                               <  1:7165814‑M1/47‑1 (MQ=255)
                                        ccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCgg                                                                                                            <  1:1194965‑M1/50‑1 (MQ=255)
                                        ccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCgg                                                                                                            <  2:148536‑M1/50‑1 (MQ=255)
                                         cgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGc                                                                                                           >  1:3006925‑M1/101‑151 (MQ=255)
                                          gcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCa                                                                                                          <  1:7070137‑M1/52‑1 (MQ=255)
                                           cgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCAc                                                                                                         <  2:1077323‑M1/53‑1 (MQ=255)
                                            gccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCAcc                                                                                                        <  2:2284278‑M1/54‑1 (MQ=255)
                                             ccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCg                                                                                                       <  1:2184011‑M1/55‑1 (MQ=255)
                                              cggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGt                                                                                                      <  1:3199147‑M1/56‑1 (MQ=255)
                                              cggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGt                                                                                                      <  2:1906814‑M1/56‑1 (MQ=255)
                                               ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTc                                                                                                     >  1:1599663‑M1/95‑151 (MQ=255)
                                               ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTc                                                                                                     >  2:6135739‑M1/95‑151 (MQ=255)
                                               ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCCTGCAGGTGCGCGACGCCGCCGGCAACATCCTCGCGACCGGCACCGTc                                                                                                     <  2:2376458‑M1/57‑1 (MQ=255)
                                                 acatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGc                                                                                                   <  2:1980366‑M1/59‑1 (MQ=255)
                                                  catcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCt                                                                                                  >  2:3523275‑M1/92‑151 (MQ=255)
                                                  catcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCt                                                                                                  >  1:2598550‑M1/92‑151 (MQ=255)
                                                   atcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTg                                                                                                 <  2:6025197‑M1/61‑1 (MQ=255)
                                                   atcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTg                                                                                                 >  2:3203914‑M1/91‑151 (MQ=255)
                                                   atcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTg                                                                                                 <  2:2903210‑M1/61‑1 (MQ=255)
                                                    tcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGc                                                                                                >  1:4187851‑M1/90‑151 (MQ=255)
                                                     cgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCCCCTGCGCTGCa                                                                                               <  1:1949979‑M1/63‑1 (MQ=255)
                                                      gacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAg                                                                                              >  1:3247470‑M1/88‑151 (MQ=255)
                                                      gacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGCGACGCCGGCGGCACCGTCCTCGCGACCGGCGCCGTCGCtgccg                                                                                              <  2:6884830‑M1/64‑3 (MQ=255)
                                                        cggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGAt                                                                                            >  1:6336414‑M1/86‑151 (MQ=255)
                                                         ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                                                                                           <  1:1013858‑M1/67‑1 (MQ=255)
                                                         ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                                                                                           >  1:892261‑M1/85‑151 (MQ=255)
                                                         ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                                                                                           <  2:171681‑M1/67‑1 (MQ=255)
                                                         ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                                                                                           >  2:1384089‑M1/85‑151 (MQ=255)
                                                          gcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATgg                                                                                          >  1:6723401‑M1/84‑151 (MQ=255)
                                                            ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGgcg                                                                                        <  1:4723754‑M1/70‑2 (MQ=255)
                                                            ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                                                                                        <  1:2416765‑M1/70‑1 (MQ=255)
                                                               gacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCt                                                                                     <  2:930014‑M1/73‑1 (MQ=255)
                                                               gacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCt                                                                                     <  1:4243258‑M1/73‑1 (MQ=255)
                                                               gacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCt                                                                                     <  1:6493473‑M1/73‑1 (MQ=255)
                                                                acacagcgccaccggaggccccgaccgatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCtt                                                                                    >  2:738405‑M1/78‑151 (MQ=255)
                                                                acacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCtt                                                                                    >  2:591848‑M1/78‑151 (MQ=255)
                                                                 cacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTc                                                                                   >  1:5603182‑M1/77‑151 (MQ=255)
                                                                  acaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCa                                                                                  <  2:4502174‑M1/76‑1 (MQ=255)
                                                                    aacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTtcacc                                                                                <  1:6091163‑M1/78‑3 (MQ=255)
                                                                       gccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGCCCGGCACCGTCGCTGCCGATGGCAccttcaccatc                                                                             <  2:3250819‑M1/81‑6 (MQ=255)
                                                                       gccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCAccttcaccatc                                                                             >  1:656191‑M1/71‑146 (MQ=255)
                                                                        ccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCaccttcaccatca                                                                            <  1:6633479‑M1/82‑7 (MQ=255)
                                                                         caccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAAGCAAAATCCTCTCGACCGGCACCGTCGCTGCAGATGGCACCTTCACCCTCAc                                                                           <  1:372696‑M1/83‑1 (MQ=255)
                                                                             ggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCACCatcaccctg                                                                       <  2:433990‑M1/87‑5 (MQ=255)
                                                                                agcACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggac                                                                    <  1:150305‑M2/150‑15 (MQ=255)
                                                                                 gCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggacc                                                                   <  2:3924604‑M2/151‑16 (MQ=255)
                                                                                  cACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCCCGTTCGccctcgccctgtcccc                                                                  <  2:2453707‑M2/151‑17 (MQ=255)
                                                                                  cACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccc                                                                  <  1:515247‑M2/151‑17 (MQ=255)
                                                                                   aCCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccct                                                                 >  2:6674940‑M2/1‑134 (MQ=255)
                                                                                    ccGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctg                                                                >  1:3908885‑M2/1‑133 (MQ=255)
                                                                                     cGACCAAGCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgc                                                               >  2:2589422‑M2/1‑132 (MQ=255)
                                                                                       agcaaTCTGGTGATCGGCCTTGCCGGCAGCCACCTCGGGGGTCGTGGCGAGGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACGTCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccg                                                             >  2:2767987‑M2/3‑130 (MQ=255)
                                                                                       aCCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCACCATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccc                                                             <  2:1830642‑M2/151‑22 (MQ=255)
                                                                                       aCCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccg                                                             >  1:5423577‑M2/1‑130 (MQ=255)
                                                                                       aCCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccg                                                             >  2:2830687‑M2/1‑130 (MQ=255)
                                                                                        ccAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgt                                                            >  2:4948334‑M2/1‑129 (MQ=255)
                                                                                          aaTCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtga                                                          <  1:431370‑M2/151‑25 (MQ=255)
                                                                                           aTCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgaa                                                         >  2:793616‑M2/1‑126 (MQ=255)
                                                                                             cTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgaatg                                                       <  2:3740124‑M2/151‑28 (MQ=255)
                                                                                                                      aaCTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgactgacggcagcaccttgcaggtcaccct                              <  2:4178896‑M2/151‑53 (MQ=255)
                                                                                                                                               caccacCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgaatgacggcagcaccttgcaggtcaccctcgccgatgccgccggcaacgtatcc     <  1:4520394‑M2/151‑78 (MQ=255)
                                                                                                                                                 ccaccGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgaatgacggcagcaccttgcaggtcaccctcgccgatgccgccggcaacgtatccca   >  1:4891300‑M2/1‑72 (MQ=255)
                                                                                                                                                  caccGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCGtcatcgccctggaccctgccgtgaatgacggcagcaccttgcaggtcaccctcgccgatgccgccggcaacgtatcccag  >  1:22919‑M2/1‑71 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCCGGGGCGAGATCGATCAGGAAGGTGCCGTTGGCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCAGCGTCACGCTGTTCCCCGCCCAGGCCAATGGTGAAGCACTCGATGTGCGTCTGGTGGATGCCGCCGGCACCGGCTTCACC  >  CP009974/1155836‑1156132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑