Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,158,307 G→A intergenic (+1830/‑13453) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,158,3070GA76.4% 887.9 / 294.1 498intergenic (+1830/‑13453)RPPX_05120/RPPX_05130hypothetical protein/channel protein TolC
Reads supporting (aligned to +/- strand):  ref base G (59/58);  new base A (176/203);  total (237/261)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 518 total aligned reads displayed.

CCGAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGC  >  CP009974/1158159‑1158457
                                                                                                                                                    |                                                                                                                                                      
ccGAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAact                                                                                                                                                      <  1:5200156/151‑1 (MQ=255)
ccGAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGCCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAact                                                                                                                                                      >  2:1253198/1‑151 (MQ=255)
 cGAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAactg                                                                                                                                                     >  1:1339002/1‑151 (MQ=255)
  gAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAactgc                                                                                                                                                    <  2:4991685/151‑1 (MQ=255)
  gAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAactgc                                                                                                                                                    >  2:3275946/1‑151 (MQ=255)
  gAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAactgc                                                                                                                                                    >  1:1965282/1‑151 (MQ=255)
           agcagcaTCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTgg                                                                                                                                           <  1:2309332/151‑1 (MQ=255)
             cagcaTCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTc                                                                                                                                         <  2:6049104/151‑1 (MQ=255)
               gcaTCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGc                                                                                                                                       >  1:2239814/1‑151 (MQ=255)
                  tCAGCGGTGCCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCAt                                                                                                                                    <  2:4174886/151‑1 (MQ=255)
                  tCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCAt                                                                                                                                    >  1:2644239/1‑151 (MQ=255)
                          aCCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGCCCCAGGCCAACGGCGAACTGCTGGACGCGGTGGCCATCGATGAcg                                                                                                                            >  2:5870443/1‑151 (MQ=255)
                             gCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGacgacg                                                                                                                         >  1:6462182/1‑151 (MQ=255)
                             gCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGAcggcg                                                                                                                         <  2:6518536/151‑1 (MQ=255)
                              cACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGAcggcgg                                                                                                                        <  1:5609092/151‑1 (MQ=255)
                              cACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGCACGCGGGCGCCAGCGCTGAcggcgg                                                                                                                        >  2:4636673/1‑151 (MQ=14)
                                 cTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGCCGGCGGCGt                                                                                                                     >  2:2742179/1‑151 (MQ=255)
                                 cTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGt                                                                                                                     <  2:6219514/151‑1 (MQ=255)
                                  tGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGtt                                                                                                                    <  1:4813920/151‑1 (MQ=255)
                                     aGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTcc                                                                                                                 <  1:163167/151‑1 (MQ=255)
                                      ggCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCt                                                                                                                <  1:4721019/151‑1 (MQ=255)
                                       gCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTc                                                                                                               <  1:6027001/151‑1 (MQ=255)
                                             gtgtGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGcc                                                                                                         <  1:3158033/151‑1 (MQ=255)
                                                 ggAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGca                                                                                                     <  2:4039561/151‑1 (MQ=255)
                                                         aTGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCAc                                                                                             <  1:1454262/151‑1 (MQ=255)
                                                         aTGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCAc                                                                                             <  2:1072541/151‑1 (MQ=255)
                                                          tGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCAcc                                                                                            >  1:4044211/1‑151 (MQ=255)
                                                          agacgacAACGGCACACTGACTTGCACTGTGGTGGCTGGCGCCGACAGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTAGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCAcc                                                                                            <  1:3822910/150‑1 (MQ=11)
                                                            aCGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACcgc                                                                                          <  1:2449827/151‑1 (MQ=255)
                                                            aCGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACcgc                                                                                          >  1:3763409/1‑151 (MQ=255)
                                                             cGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACcgcg                                                                                         >  1:32980/1‑151 (MQ=255)
                                                                   cGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGt                                                                                                                                             <  2:3443540/93‑1 (MQ=14)
                                                                   cGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGt                                                                                                                                             >  1:3443540/1‑93 (MQ=14)
                                                                    ggCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACa                                                                                  >  1:5041461/1‑151 (MQ=255)
                                                                        cacTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCAc                                                                              <  1:5074027/151‑1 (MQ=255)
                                                                               tGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTCTCCTCGCTGCCCGCACAGATCACCGCGCCCGCCATCCCCGccccc                                                                       >  2:2051377/1‑150 (MQ=11)
                                                                                  cACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGcc                                                                    <  1:3702265/151‑1 (MQ=9)
                                                                                  cACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGcc                                                                    <  1:1109269/151‑1 (MQ=9)
                                                                                  cACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGcc                                                                    <  2:7035067/151‑1 (MQ=9)
                                                                                                       cGGCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATc                                               >  1:7182714/1‑151 (MQ=2)
                                                                                                        ggCAGCTTCACCGTCGCCGTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCa                                              <  2:6619215/151‑1 (MQ=255)
                                                                                                         gCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCaa                                             <  2:3676181/151‑1 (MQ=9)
                                                                                                         gCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCaa                                             <  2:3894453/151‑1 (MQ=9)
                                                                                                          cAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAAt                                            <  1:1258246/151‑1 (MQ=9)
                                                                                                           aGCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATg                                           <  1:7070977/151‑1 (MQ=9)
                                                                                                            gcgtCACCGTCCCCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGc                                          <  2:3063649/148‑1 (MQ=255)
                                                                                                            gCTTCACCGTCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGc                                          <  1:671963/151‑1 (MQ=255)
                                                                                                            gCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGc                                          <  1:5917352/151‑1 (MQ=12)
                                                                                                             cTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGcc                                         <  2:1158707/151‑1 (MQ=255)
                                                                                                               tCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCACGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGa                                       <  1:5434989/151‑1 (MQ=12)
                                                                                                                cACCGTGACTCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCAGCGATGACGGCGGCGTGTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGAc                                      <  2:5667056/151‑1 (MQ=11)
                                                                                                                cACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCCCCGACCGAGCTGGTGCTCAATGccccc                                      >  2:6347260/1‑148 (MQ=11)
                                                                                                                cACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGCCGCCATCGATGACGGCGGCGCTTCCTCGCTGCCCGCACAGATCACCGCGCCCGCCATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGAc                                      >  2:2264552/1‑151 (MQ=11)
                                                                                                                 cccGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACg                                     <  1:3338701/150‑1 (MQ=255)
                                                                                                                 aCCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACg                                     >  1:2999996/1‑151 (MQ=255)
                                                                                                                   cGTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGc                                   <  1:5771066/151‑1 (MQ=12)
                                                                                                                    gTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCa                                  <  1:6306004/151‑1 (MQ=9)
                                                                                                                    gTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCa                                  >  1:3061970/1‑151 (MQ=9)
                                                                                                                    gTCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCa                                  >  2:140462/1‑151 (MQ=9)
                                                                                                                     tCACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAg                                 >  1:5345594/1‑151 (MQ=12)
                                                                                                                     tCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAg                                 <  2:2966721/151‑1 (MQ=12)
                                                                                                                     tCACCCTCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAg                                 >  1:4231227/1‑151 (MQ=9)
                                                                                                                      cACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGCCGGCAGc                                >  2:862892/1‑151 (MQ=255)
                                                                                                                      cACCCTCGACCCGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGc                                <  1:1386935/151‑1 (MQ=12)
                                                                                                                      cACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACCTCACCGCCCCCGCCGCCCCGACCCAGCTGGCGATCAATGCCCACGGCAGc                                >  2:5799717/1‑151 (MQ=11)
                                                                                                                        cccTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGt                              >  1:1489751/1‑151 (MQ=12)
                                                                                                                         ccTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTg                             <  2:1596742/151‑1 (MQ=12)
                                                                                                                           tCGACCCGCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGt                           <  1:1068669/151‑1 (MQ=255)
                                                                                                                            cGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTc                          <  1:6942792/151‑1 (MQ=12)
                                                                                                                              ccccGGCCCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCAc                        <  1:3868113/150‑1 (MQ=9)
                                                                                                                              ccccGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCAc                        <  2:546322/150‑1 (MQ=12)
                                                                                                                              aCCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCAc                        <  1:2111543/151‑1 (MQ=12)
                                                                                                                               cccggcccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCAcc                       >  1:4331900/8‑151 (MQ=9)
                                                                                                                               cccggcccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCAcc                       <  1:4916469/144‑1 (MQ=12)
                                                                                                                                ccggcccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGACGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCg                      <  2:6953945/145‑1 (MQ=9)
                                                                                                                                ccGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCg                      <  2:3377286/151‑1 (MQ=17)
                                                                                                                                 cagcccgggCCGACGGCGAACTGCTGGACGTGGTCTCAATCGATGGCGGCGGCGTTGCCTCGCTGCCTGCCCAGACCCCCGCGCCCGCCGTCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCgg                     <  2:3166089/144‑1 (MQ=11)
                                                                                                                                  gCCTCAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGc                    >  2:1908827/1‑151 (MQ=12)
                                                                                                                                   gcccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGcc                   <  1:1009346/148‑1 (MQ=17)
                                                                                                                                   gcccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGcc                   <  1:2668681/148‑1 (MQ=17)
                                                                                                                                   ccTCAGGCCCACGGCGAACTGCTGGACGCGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGcc                   <  1:4444094/151‑1 (MQ=11)
                                                                                                                                     gcAGGCCAACGGCGCGCTGGTGGCCGTGGCCGCCATCGATGACGGCGGCGCCTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGt                 <  2:726593/150‑1 (MQ=11)
                                                                                                                                     ccAGGCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCgc                 <  1:1838960/150‑2 (MQ=14)
                                                                                                                                     acaagCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGt                 >  1:4095650/5‑151 (MQ=11)
                                                                                                                                      cAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTg                >  2:5257065/1‑151 (MQ=18)
                                                                                                                                       aagCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGc               <  2:448325/149‑1 (MQ=255)
                                                                                                                                       aGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGCCGGCGGCGTTTCCTCGCTGCCCGCGCAGATCACCGCGCCCGGCAGCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGc               <  2:3539831/151‑1 (MQ=11)
                                                                                                                                        ggCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGcc              >  1:4556703/1‑151 (MQ=19)
                                                                                                                                        agCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCTCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGcc              >  1:5989049/2‑151 (MQ=11)
                                                                                                                                        agCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGcc              <  1:832133/150‑1 (MQ=255)
                                                                                                                                         gCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCg             <  1:1734098/151‑1 (MQ=19)
                                                                                                                                         gCCAACGGCGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCg             <  1:617169/151‑1 (MQ=255)
                                                                                                                                           cAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGaa           <  2:1704418/151‑1 (MQ=255)
                                                                                                                                             aCGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAAcc         <  2:241984/151‑1 (MQ=255)
                                                                                                                                              cGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCg        >  1:322488/1‑151 (MQ=255)
                                                                                                                                                 cGAGCTGCTGGACGTGGTCGCCATCGATGCCGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGgc     <  2:1845123/151‑1 (MQ=255)
                                                                                                                                                 cGAACTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGgc     >  1:2787433/1‑151 (MQ=255)
                                                                                                                                                  gAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGgca    >  1:1568398/1‑151 (MQ=255)
                                                                                                                                                    gctgctGGACGTGGTCGCCATGGATGAGGGCGGCGGTTCCTCGCTGCCCGCACAGATCACCGCGCCCGAGATCGCCGCCCCTGCCGCACCGCCCGGGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGgcagc  <  2:4321212/151‑1 (MQ=11)
                                                                                                                                                    gctgcgGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGACCGAGCTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGgcagc  <  2:5299160/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CCGAAGACGGCAGCAGCATCAGCGGTACCGCACCTGCAGGCACCCGTGTGGAAGTGCATGACGCCAACGGCACACTGATTGGCACTGTGGTGGCTGGCCCCGACGGCAGCTTCACCGTCACCCTCGACCCGCCTCAGGCCAACGGCGAGCTGCTGGACGTGGTCGCCATCGATGACGGCGGCGTTTCCTCGCTGCCCGCACAGATCACCGCGCCCGACATCACCGCCCCTGCCGCACCGAGCGAACTGGTGATCAATGCCGACGGCAGCGTGGTCACCGGCCGTGCCGAACCGGGCAGC  >  CP009974/1158159‑1158457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑