BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,345	A→T	V354V (GTT→GTA)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,345	0	A	T	99.4%	465.7 / NA	170	V354V (GTT→GTA)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (93/76); minor base C (1/0); total (94/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATAACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTG  >  CP009974/1554197‑1554492

tCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTAc                                                                                                                                                   <  2:2292085/151‑1 (MQ=255)

tCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTAc                                                                                                                                                   >  2:2318068/1‑151 (MQ=255)

  aaTCGGTCGAGTGATTACTCGATGATTTTTTCCTCAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCACGGTAACAGTCATCTGAATGTTTTCACCTGGCCTTAccc                                                                                                                                                 >  2:4845484/1‑150 (MQ=17)

  aaTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCa                                                                                                                                                 >  1:887169/1‑151 (MQ=255)

   aTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAt                                                                                                                                                <  2:2767890/151‑1 (MQ=255)

   aTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAt                                                                                                                                                >  1:3686125/1‑151 (MQ=255)

     cGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAttt                                                                                                                                              <  1:6128705/151‑1 (MQ=255)

      ggTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTc                                                                                                                                             >  1:2036539/1‑151 (MQ=255)

      ggTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTc                                                                                                                                             >  1:2782323/1‑151 (MQ=255)

       gTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCa                                                                                                                                            <  2:6172302/151‑1 (MQ=255)

        tCGAGTGATTACTCGATGATTTTTGTCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                           >  2:3077925/1‑151 (MQ=255)

        tCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                           >  1:4086967/1‑151 (MQ=255)

         cGAGTTATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                          <  2:5277456/151‑1 (MQ=255)

         cGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                          <  2:1658871/151‑1 (MQ=255)

         cGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                          >  2:7235052/1‑151 (MQ=255)

          gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                         >  1:6398757/1‑151 (MQ=255)

          gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                         >  1:2555361/1‑151 (MQ=255)

             tGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCt                                                                                                                                      <  1:6444255/151‑1 (MQ=255)

             tGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGCAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCt                                                                                                                                      >  2:4914277/1‑151 (MQ=255)

              gATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCtt                                                                                                                                     <  1:5772123/151‑1 (MQ=255)

              gATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCtt                                                                                                                                     >  1:5729171/1‑151 (MQ=255)

               aTTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTc                                                                                                                                    >  1:901056/1‑151 (MQ=255)

               aTTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTc                                                                                                                                    <  1:226685/151‑1 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                   >  1:5012496/1‑151 (MQ=255)

                ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                   >  2:1843354/1‑151 (MQ=255)

                  aCTCGATGGTTTTTGCCACAACGCCGGCGCCGACGGTAGTACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCgg                                                                                                                                 <  2:2431163/151‑1 (MQ=255)

                   cTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCACTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTCGTCACCTGGCATCACCATTTCAACGCCttactgc                                                                                                                                >  2:6389294/1‑146 (MQ=255)

                    tCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCa                                                                                                                               <  2:3927473/151‑1 (MQ=38)

                     cGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTCGCATTACCATTTCAACGCCTTCCGGccg                                                                                                                              >  2:6413558/1‑149 (MQ=255)

                      gatgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGt                                                                                                                             <  2:2171198/151‑1 (MQ=38)

                      gatgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGt                                                                                                                             <  1:5146165/151‑1 (MQ=255)

                       atgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                            >  1:1064235/1‑151 (MQ=38)

                       atgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                            >  1:4675868/1‑151 (MQ=38)

                       atgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                            <  1:4344516/151‑1 (MQ=37)

                        tgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTc                                                                                                                           >  2:6249147/1‑151 (MQ=37)

                        tgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTc                                                                                                                           <  1:965077/151‑1 (MQ=25)

                         gatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCg                                                                                                                          >  2:412757/1‑151 (MQ=25)

                         gatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCg                                                                                                                          <  1:3901696/151‑1 (MQ=25)

                          atTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGc                                                                                                                         <  1:1185438/151‑1 (MQ=21)

                             ttggCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCGTCCGGCAGTTCGCAGt                                                                                                                      <  1:4839931/148‑1 (MQ=16)

                              ttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGtt                                                                                                                     <  2:4503409/151‑1 (MQ=18)

                               tGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTa                                                                                                                    >  2:3505825/1‑151 (MQ=21)

                               tGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTa                                                                                                                    >  2:4986921/1‑151 (MQ=21)

                                gCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAc                                                                                                                   <  1:3842681/151‑1 (MQ=18)

                                 ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                  >  1:6211724/1‑151 (MQ=18)

                                 ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                  >  2:3408387/1‑151 (MQ=18)

                                 ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                  >  1:5568871/1‑151 (MQ=18)

                                  cacaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCg                                                                                                                 >  2:2514216/1‑151 (MQ=17)

                                  cacaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCg                                                                                                                 <  1:1087019/151‑1 (MQ=17)

                                  cacaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCACTTACCg                                                                                                                 >  1:6256879/1‑151 (MQ=17)

                                   acaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCgg                                                                                                                <  1:3559221/151‑1 (MQ=14)

                                     aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                              <  2:3263076/151‑1 (MQ=11)

                                     aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                              <  2:3266240/151‑1 (MQ=11)

                                     aaCGCCGGCGCCGACGGGACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                              <  2:6473677/151‑1 (MQ=11)

                                                                                                           ttGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttctt                                        >  2:1286853/1‑151 (MQ=14)

                                                                                                           ttGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttctt                                        >  1:279465/1‑151 (MQ=14)

                                                                                                           ttGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttctt                                        <  2:195171/151‑1 (MQ=14)

                                                                                                           ttGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttctt                                        <  1:3171176/151‑1 (MQ=14)

                                                                                                            tGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCttcttc                                       >  1:4599683/1‑151 (MQ=14)

                                                                                                            tGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACGGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCtgcttc                                       >  1:6791365/1‑151 (MQ=9)

                                                                                                            tGATCAGGGTAACAGTCAGCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCttcttc                                       <  2:4052034/151‑1 (MQ=14)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      <  1:5643315/151‑1 (MQ=14)

                                                                                                             gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                      >  1:3051943/1‑151 (MQ=14)

                                                                                                              aTCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcct                                     >  2:7002437/1‑151 (MQ=14)

                                                                                                              aTCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcct                                     >  2:7002528/1‑151 (MQ=14)

                                                                                                                cAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                   >  2:56355/1‑151 (MQ=14)

                                                                                                                cAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                   <  1:1484060/151‑1 (MQ=17)

                                                                                                                cAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                   >  1:449456/1‑151 (MQ=17)

                                                                                                                   ggTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGAc                                >  1:5907421/1‑151 (MQ=17)

                                                                                                                   ggTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGAc                                >  1:7120446/1‑151 (MQ=14)

                                                                                                                    gTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGCCa                               >  1:6128231/1‑151 (MQ=17)

                                                                                                                      aaCAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAgg                             <  2:551562/151‑1 (MQ=17)

                                                                                                                        cAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGAc                           <  1:755707/151‑1 (MQ=17)

                                                                                                                          gTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGt                         <  1:5385951/151‑1 (MQ=17)

                                                                                                                          gTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGGGTGACGGCCGCCTTCTTCCTTCGACAGGAcaa                         >  2:5001450/1‑149 (MQ=9)

                                                                                                                           tCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTa                        <  1:2719454/151‑1 (MQ=17)

                                                                                                                            cATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAg                       >  2:3524984/1‑151 (MQ=17)

                                                                                                                            cATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAg                       <  2:5349328/151‑1 (MQ=17)

                                                                                                                             accTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGa                      <  1:5972541/149‑1 (MQ=17)

                                                                                                                               cTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGt                         >  2:137720/1‑146 (MQ=17)

                                                                                                                                 gAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTc                  >  2:3868651/1‑151 (MQ=14)

                                                                                                                                 gAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTc                  >  2:2856643/1‑151 (MQ=17)

                                                                                                                                  aaTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTGGACTTCt                 >  2:4453850/1‑151 (MQ=17)

                                                                                                                                  aaTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCt                 >  2:6453074/1‑151 (MQ=17)

                                                                                                                                   aTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCtc                >  1:4977693/1‑150 (MQ=14)

                                                                                                                                   aTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTg                >  2:847186/1‑151 (MQ=17)

                                                                                                                                    tgttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGc               <  2:5363579/151‑1 (MQ=17)

                                                                                                                                    tgttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGc               <  1:4756422/151‑1 (MQ=17)

                                                                                                                                      ttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCgg             >  1:2630565/1‑151 (MQ=17)

                                                                                                                                        gtCACCTGGCATTCCCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTg           <  1:6980660/151‑1 (MQ=17)

                                                                                                                                        gtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTg           <  2:5605965/151‑1 (MQ=17)

                                                                                                                                         tCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGa          <  2:6711436/151‑1 (MQ=17)

                                                                                                                                          cACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGaa         <  2:5720406/151‑1 (MQ=17)

                                                                                                                                          cACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGaa         <  2:1100285/151‑1 (MQ=17)

                                                                                                                                            ccTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGACGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTCGACTCCTGCGGTGAAcg       >  2:4678996/1‑150 (MQ=255)

                                                                                                                                            ccTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACt       >  2:344275/1‑151 (MQ=14)

                                                                                                                                             cTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACtt      >  1:2964179/1‑151 (MQ=14)

                                                                                                                                             cTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACtt      <  2:4623775/151‑1 (MQ=17)

                                                                                                                                                gCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGgt   >  1:1815333/1‑151 (MQ=17)

                                                                                                                                                 cATTACCTTTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGgtg  <  2:4455386/151‑1 (MQ=14)

TCAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATAACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTG  >  CP009974/1554197‑1554492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`