BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,555,277	T→C	I44V (ATC→GTC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,555,277	0	T	C	99.3%	662.6 / NA	295	I44V (ATC→GTC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (141/151); minor base A (1/1); total (143/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

ATCATGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGC  >  CP009974/1555129‑1555423

aTCATGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTg                                                                                                                                                  >  1:1054049/1‑151 (MQ=18)

  cATGTTCGTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGcc                                                                                                                                                <  2:6529518/151‑1 (MQ=18)

   aTGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccg                                                                                                                                               >  2:2025535/1‑151 (MQ=21)

    tGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccga                                                                                                                                              <  1:1483313/151‑1 (MQ=18)

        cttcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaaccg                                                                                                                                          >  1:6958401/1‑151 (MQ=17)

        cttcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaaccg                                                                                                                                          <  1:4690455/151‑1 (MQ=17)

        cttcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaaccg                                                                                                                                          >  1:3109936/1‑151 (MQ=17)

         ttcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaaccga                                                                                                                                         >  2:1424039/1‑151 (MQ=17)

          tcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccggaccgaa                                                                                                                                        >  2:267534/1‑151 (MQ=14)

             cGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAAc                                                                                                                                     >  2:6883772/1‑151 (MQ=17)

              gTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACt                                                                                                                                    >  1:3026388/1‑151 (MQ=17)

               tAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACtt                                                                                                                                   <  2:2806312/151‑1 (MQ=17)

               tAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCCAAAACtt                                                                                                                                   >  2:6153360/1‑151 (MQ=17)

               tAGTCAGAGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACtt                                                                                                                                   <  2:4452060/151‑1 (MQ=17)

                aGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATTTTCGAGTTGGACTCGACGTGAGCGGTGTTGATGGTAATACCACGCGCTTTTTCTCCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTc                                                                                                                                  <  2:7078347/151‑1 (MQ=11)

                aGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTc                                                                                                                                  <  1:4768057/151‑1 (MQ=14)

                aGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTc                                                                                                                                  >  2:1917811/1‑151 (MQ=17)

                 gTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACttag                                                                                                                                 <  2:316396/151‑3 (MQ=17)

                    aGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAg                                                                                                                              <  2:3586622/151‑1 (MQ=17)

                     gCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGc                                                                                                                             <  2:17296/151‑1 (MQ=17)

                     gCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGc                                                                                                                             <  1:2158350/151‑1 (MQ=17)

                      cGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGATCGGTGTTGATGGTGATACCGCTCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCCACGACTGCCGAACCGAAAACTTCGGTGcg                                                                                                                            >  1:960515/1‑150 (MQ=11)

                      cGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCa                                                                                                                            >  2:3300113/1‑151 (MQ=17)

                      cGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTGGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAGAACATCgcagca                                                                                                                            >  2:4637905/1‑151 (MQ=255)

                        tGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCaga                                                                                                                          <  2:10753/151‑3 (MQ=17)

                           ccTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAcgc                                                                                                                       >  1:745218/1‑151 (MQ=12)

                              gggCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAg                                                                                                                    >  2:1319706/1‑151 (MQ=17)

                                gCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTc                                                                                                                  <  1:2686124/151‑1 (MQ=9)

                                 cAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCa                                                                                                                 <  1:1946551/151‑1 (MQ=12)

                                      aaCGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCt                                                                                                            <  2:793873/151‑1 (MQ=12)

                                        cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGc                                                                                                          <  2:4821149/151‑1 (MQ=12)

                                        cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGc                                                                                                          <  2:2350309/151‑1 (MQ=12)

                                        cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGc                                                                                                          >  1:6696685/1‑151 (MQ=12)

                                         gTGAGCGTAGTTACGAATGTTCGAGTTGTACTCGAGGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                         <  2:4338525/151‑1 (MQ=255)

                                         gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                         <  2:7191136/151‑1 (MQ=14)

                                         gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                         >  2:3975107/1‑151 (MQ=17)

                                           gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGt                                                                                                       >  1:6195475/1‑151 (MQ=12)

                                                tAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCagag                                                                                                  >  1:1094093/1‑151 (MQ=12)

                                                 aGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCagagg                                                                                                 >  1:6024491/1‑150 (MQ=12)

                                                    gACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCTAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACCTCGGCGCAGACGCGAGTCAGAGCTGCGGCCAGAGTggc                                                                                              >  2:5036457/1‑150 (MQ=11)

                                                    gACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGt                                                                                              >  1:4785326/1‑151 (MQ=12)

                                                      cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                            >  1:3468809/1‑151 (MQ=17)

                                                      cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                            <  2:1539843/151‑1 (MQ=12)

                                                      cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                            >  1:457480/1‑151 (MQ=17)

                                                       gAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCtt                                                                                           >  1:4183631/1‑151 (MQ=12)

                                                         aTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTAc                                                                                         >  1:3426531/1‑151 (MQ=14)

                                                            ttCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGt                                                                                      <  1:824711/151‑1 (MQ=11)

                                                                             gTGGGCGGTGTTGATGGTGAGACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                     <  2:5443760/151‑1 (MQ=2)

                                                                             gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCACCGTGGCCGATg                                                                     >  2:332216/1‑151 (MQ=2)

                                                                             gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                     <  1:7150392/151‑1 (MQ=2)

                                                                             gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                     <  1:1544673/151‑1 (MQ=2)

                                                                             gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                     <  1:4398856/151‑1 (MQ=2)

                                                                             gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                     <  1:4002731/151‑1 (MQ=2)

                                                                               gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                   <  1:6565996/151‑1 (MQ=9)

                                                                                aGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                  <  1:6672724/151‑1 (MQ=14)

                                                                                 gCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGTAGTCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACGTCGGAGCAGACGCGAGCCAGAGCTGCGGCCAGAGAGGTCTTACCGTGGTCAACGTGGCCGATGGTGc                                                                 >  2:2237482/1‑151 (MQ=11)

                                                                                  cGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGcc                                                                <  1:5320028/151‑1 (MQ=12)

                                                                                    gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                              <  2:6787151/151‑1 (MQ=12)

                                                                                      gtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACg                                                            >  1:5242517/1‑151 (MQ=12)

                                                                                      gtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGAGCCGACg                                                            >  1:2474904/1‑151 (MQ=12)

                                                                                        tGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGtt                                                          <  1:5855338/151‑1 (MQ=12)

                                                                                          aTGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTaa                                                        >  2:1260213/1‑151 (MQ=14)

                                                                                            ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                      <  1:3982236/151‑1 (MQ=17)

                                                                                            ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                      <  1:3707704/151‑1 (MQ=12)

                                                                                            ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                      >  1:2863239/1‑151 (MQ=12)

                                                                                                 tACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTgggg                                                 >  2:6252417/1‑151 (MQ=9)

                                                                                                    cgcgcgCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaag                                              >  2:5877116/1‑151 (MQ=14)

                                                                                                     gcgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagg                                             <  2:4092694/151‑1 (MQ=17)

                                                                                                     gcgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagg                                             <  2:2109041/151‑1 (MQ=17)

                                                                                                         gcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGTGAAc                                         <  2:5976583/151‑1 (MQ=12)

                                                                                                          cTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACg                                        <  1:5685842/151‑1 (MQ=12)

                                                                                                           tttttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGa                                       >  2:335331/1‑151 (MQ=12)

                                                                                                           tttttCGTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGa                                       <  2:5762349/151‑1 (MQ=12)

                                                                                                                cttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaaa                                  >  1:2434277/1‑151 (MQ=12)

                                                                                                                cttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaaa                                  <  2:3426531/151‑1 (MQ=12)

                                                                                                                cttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaaa                                  >  2:1150120/1‑151 (MQ=9)

                                                                                                                  tcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAtt                                >  1:1235222/1‑151 (MQ=16)

                                                                                                                  tcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAtt                                <  2:5711173/151‑1 (MQ=16)

                                                                                                                     ggAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAttttt                             >  2:4230221/1‑151 (MQ=16)

                                                                                                                       aGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACGTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCt                           <  2:6659688/151‑1 (MQ=255)

                                                                                                                        gCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCtt                          <  2:5121023/151‑1 (MQ=255)

                                                                                                                         ccGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCttt                         <  2:4561457/151‑1 (MQ=255)

                                                                                                                            aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGc                      <  1:1752570/151‑1 (MQ=255)

                                                                                                                                gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                  >  1:6459752/1‑151 (MQ=255)

                                                                                                                                gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                  >  2:297316/1‑151 (MQ=255)

                                                                                                                                gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                  >  1:6754488/1‑151 (MQ=255)

                                                                                                                                 aTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGa                 <  1:5863047/151‑1 (MQ=255)

                                                                                                                                 aTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGa                 >  1:3839443/1‑151 (MQ=255)

                                                                                                                                   cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa               <  1:3282254/151‑1 (MQ=255)

                                                                                                                                   cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa               >  1:7057526/1‑151 (MQ=255)

                                                                                                                                    ttGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAg              >  1:6839569/1‑151 (MQ=255)

                                                                                                                                      gTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTg            <  1:5015081/151‑1 (MQ=255)

                                                                                                                                      gTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTg            <  1:4865792/151‑1 (MQ=255)

                                                                                                                                           aCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACct       >  1:5148890/1‑151 (MQ=255)

                                                                                                                                            cTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACctc      <  2:3468809/151‑1 (MQ=255)

                                                                                                                                             tCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACctct     >  2:6188703/1‑151 (MQ=255)

                                                                                                                                              gagcGACTGCCGAACCGAAAACTTCGGAGCAGAGGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTCGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAACTTTTTCTTTAGCCACGACAGTGAACCTCtt    <  2:4327935/148‑1 (MQ=17)

                                                                                                                                              cAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCtt    >  2:5297493/1‑151 (MQ=255)

                                                                                                                                               aaCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTg   >  2:5246199/1‑151 (MQ=255)

                                                                                                                                                aCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGc  <  1:3573477/151‑1 (MQ=255)

ATCATGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGC  >  CP009974/1555129‑1555423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`