BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,555,302	A→G	V35V (GTT→GTC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,555,302	0	A	G	99.7%	725.1 / ‑5.4	287	V35V (GTT→GTC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (137/148); total (139/148)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 287 total aligned reads displayed.

GACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGTTTTTTA  >  CP009974/1555154‑1555449

gACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAc                                                                                                                                                   <  2:4309589/151‑1 (MQ=17)

 aCCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAcg                                                                                                                                                  >  1:6087002/1‑151 (MQ=12)

  ccTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAcgc                                                                                                                                                 >  1:745218/1‑151 (MQ=12)

     gggCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAg                                                                                                                                              >  1:4169969/1‑151 (MQ=12)

        cAGTCAACGTGCGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTAGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGacgaa                                                                                                                                           >  2:5256091/1‑147 (MQ=11)

         aGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCTGGATTTGTGTCGATCTTGTCGAACTCAACGAATGCCGAACGGATAACTTCGGAGCCTATGCGAGTCag                                                                                                                                          >  2:3396487/1‑151 (MQ=11)

         aGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCag                                                                                                                                          <  1:515816/151‑1 (MQ=14)

          gTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCaga                                                                                                                                         <  1:2372778/151‑1 (MQ=12)

            cAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGc                                                                                                                                       >  2:5881071/1‑151 (MQ=12)

             aaCGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCt                                                                                                                                      <  2:793873/151‑1 (MQ=12)

               cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGc                                                                                                                                    >  1:6696685/1‑151 (MQ=12)

                gTGAGCGTAGTTACGAATGTTCGAGTTGTACTCGAGGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                                                   <  2:4338525/151‑1 (MQ=255)

                gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                                                   <  2:5061307/151‑1 (MQ=9)

                gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                                                   <  2:7191136/151‑1 (MQ=14)

                  gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGt                                                                                                                                 <  2:251784/151‑1 (MQ=9)

                    gCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGCACCGAAAACTTCGGAGCAGACGCGTGTCAGAGCTGCGGTCa                                                                                                                               >  2:4196469/1‑151 (MQ=255)

                     cGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCag                                                                                                                              >  2:4329162/1‑151 (MQ=17)

                      gTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCTTTCGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAAGCGAAAACTTCGGAGCAGACGCGAGCCAGAGCTGCGGTCaga                                                                                                                             >  2:1342281/1‑151 (MQ=11)

                       tAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCagag                                                                                                                            >  1:1094093/1‑151 (MQ=12)

                           gACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGt                                                                                                                        >  1:1773160/1‑151 (MQ=17)

                           gACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGt                                                                                                                        >  1:4785326/1‑151 (MQ=12)

                             cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                                                      <  2:1539843/151‑1 (MQ=12)

                             cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                                                      >  1:457480/1‑151 (MQ=17)

                              gAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCtt                                                                                                                     >  1:3001740/1‑151 (MQ=12)

                              gAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCtt                                                                                                                     >  2:1883063/1‑151 (MQ=12)

                                aTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTAc                                                                                                                   >  2:6834898/1‑151 (MQ=14)

                                 tGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTAcc                                                                                                                  >  2:5200458/1‑151 (MQ=14)

                                   ttCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGt                                                                                                                <  1:824711/151‑1 (MQ=11)

                                   ttCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGCAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCCAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGt                                                                                                                <  1:49003/151‑1 (MQ=2)

                                                    gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                                               >  2:332502/1‑151 (MQ=2)

                                                    gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                                               <  1:1544673/151‑1 (MQ=2)

                                                    gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                                               <  1:7150392/151‑1 (MQ=2)

                                                    gTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATg                                                                                               >  1:4033805/1‑151 (MQ=2)

                                                     tGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                                              >  2:4887943/1‑151 (MQ=9)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATggg                                                                                             >  2:5801815/1‑150 (MQ=9)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                             <  1:6565996/151‑1 (MQ=9)

                                                         cGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGcc                                                                                          >  2:4407624/1‑151 (MQ=12)

                                                         cGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGcc                                                                                          <  1:5320028/151‑1 (MQ=12)

                                                          ggTGTTGATGGTGATACCGCGCGCTTTTTTTTCTTTTGTCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGCTGGTGCCg                                                                                         >  2:602915/1‑151 (MQ=11)

                                                             gtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACg                                                                                      >  1:5242517/1‑151 (MQ=12)

                                                               tGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGtt                                                                                    <  1:5855338/151‑1 (MQ=12)

                                                                  tGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAAc                                                                                 >  2:2200718/1‑151 (MQ=14)

                                                                   ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                                                <  1:3707704/151‑1 (MQ=12)

                                                                   ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                                                >  1:2863239/1‑151 (MQ=12)

                                                                     tGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTg                                                                              >  1:5097203/1‑151 (MQ=12)

                                                                       aTACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTggg                                                                            <  1:2717365/151‑1 (MQ=12)

                                                                       aTACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTggg                                                                            >  2:2035051/1‑151 (MQ=12)

                                                                        tACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTgggg                                                                           >  2:6252417/1‑151 (MQ=9)

                                                                           cgcgcgCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaag                                                                        <  2:4488935/151‑1 (MQ=17)

                                                                               cgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGAGGGTGCCGACGTTAACGAGgggcagggaa                                                                    >  2:2749631/1‑151 (MQ=11)

                                                                                   ttttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGCGCAGACGCGAGCCAGCGCCGCGGTCCGAGCGGTCTTACCGTGGTCAACGTGGCCGATGGCGCCGACCTTACCGTGgcgcaggccacccc                                                                >  2:7012160/1‑144 (MQ=11)

                                                                                   ttttCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGAt                                                                >  2:1290179/1‑151 (MQ=9)

                                                                                     ttcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCa                                                              >  1:3253591/1‑151 (MQ=9)

                                                                                      tcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaa                                                             >  1:3549686/1‑151 (MQ=9)

                                                                                       cttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaaa                                                            >  1:2434277/1‑151 (MQ=12)

                                                                                       cttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaaa                                                            >  1:3439676/1‑151 (MQ=12)

                                                                                         tcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAtt                                                          <  2:5711173/151‑1 (MQ=16)

                                                                                         tcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAtt                                                          >  1:1235222/1‑151 (MQ=16)

                                                                                            ggAGCCGAGTCGATCTTGTCGAACGCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAttttt                                                       <  2:3911987/151‑1 (MQ=16)

                                                                                             gAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTc                                                      <  2:3750734/151‑1 (MQ=25)

                                                                                              aGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCt                                                     <  2:4041346/151‑1 (MQ=25)

                                                                                              aGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACGTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCt                                                     <  2:6659688/151‑1 (MQ=255)

                                                                                               gcctgatCGATCTTGTCGAACACAACGACTGCCGAACCGAAAACGTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTTGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCtt                                                    <  2:4611494/145‑1 (MQ=11)

                                                                                                ccGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCttt                                                   <  2:4561457/151‑1 (MQ=255)

                                                                                                       gATCTTGTCGAACTCAACGACTGCCGCACCGAAAACTTCGGAGCAGCCGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCAATGGTGCCGACGTCAACGTGGGGCAGTGCACGATCACATTTTTCTTTAGCCCCg                                            >  2:5593633/1‑151 (MQ=11)

                                                                                                       gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                                            >  1:6459752/1‑151 (MQ=255)

                                                                                                       gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                                            >  1:6754488/1‑151 (MQ=255)

                                                                                                       gATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACg                                            >  2:3318858/1‑151 (MQ=255)

                                                                                                        aTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGa                                           <  1:5863047/151‑1 (MQ=255)

                                                                                                        aTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGa                                           >  1:3839443/1‑151 (MQ=255)

                                                                                                          cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa                                         >  1:7057526/1‑151 (MQ=255)

                                                                                                           ttGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAg                                        >  1:6839569/1‑151 (MQ=255)

                                                                                                           ttGTCGAACTCAACGACTGCCGAACCAAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAg                                        <  1:6227530/151‑1 (MQ=255)

                                                                                                              tCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGa                                     <  1:2065563/151‑1 (MQ=255)

                                                                                                                gAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAAc                                   >  2:3466461/1‑151 (MQ=255)

                                                                                                                 aaCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAAcc                                  <  1:335331/151‑1 (MQ=255)

                                                                                                                    tCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACctct                               >  2:6188703/1‑151 (MQ=255)

                                                                                                                     cAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCtt                              >  2:5297493/1‑151 (MQ=255)

                                                                                                                     cAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGCGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAGTTTTCTTTAGCCACGACAGTGAACCTCtt                              >  2:1091694/1‑151 (MQ=255)

                                                                                                                       aCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGc                            <  1:3573477/151‑1 (MQ=255)

                                                                                                                        cgactGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGcc                           <  1:1488997/147‑1 (MQ=255)

                                                                                                                         gactGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCt                          <  1:3975107/148‑1 (MQ=255)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTa                         >  1:1321331/3‑151 (MQ=255)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTa                         <  1:5656552/149‑1 (MQ=255)

                                                                                                                            tGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGAc                                          <  1:4334118/132‑1 (MQ=255)

                                                                                                                            tGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTaaa                       >  1:6259340/1‑151 (MQ=255)

                                                                                                                               cgaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAggg                    <  2:1156482/151‑1 (MQ=255)

                                                                                                                                gaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAgggg                   >  2:3586602/1‑151 (MQ=255)

                                                                                                                                gaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAgggg                   <  2:3003634/151‑1 (MQ=255)

                                                                                                                                   ccgaaAACTTCGGAGCAGACGCGGGTCAGCGCTGCGGTCAGAGTGGTCTTACCGTGGGCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGAtt                <  1:3146885/151‑1 (MQ=255)

                                                                                                                                      aaaaCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGc             >  1:3990105/1‑151 (MQ=255)

                                                                                                                                       aaaCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGcc            >  2:5391082/1‑151 (MQ=255)

                                                                                                                                           ttCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTTGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATGTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTATCCTTGt        >  2:2284874/1‑151 (MQ=255)

                                                                                                                                           ttCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGt        >  1:7231463/1‑151 (MQ=255)

                                                                                                                                           ttCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGt        >  1:2489581/1‑151 (MQ=255)

                                                                                                                                             cGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGttt      <  2:4701666/151‑1 (MQ=255)

                                                                                                                                              ggAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGtttt     >  2:6311472/1‑151 (MQ=255)

                                                                                                                                              ggAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGtttt     <  2:3534810/151‑1 (MQ=255)

                                                                                                                                               gAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGttttt    >  2:304933/1‑151 (MQ=255)

                                                                                                                                                aGCAGACGCGAGTCAGCGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATCTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGtttttt   >  2:2960653/1‑151 (MQ=255)

GACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGTTTTTTA  >  CP009974/1555154‑1555449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`