BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	2,738,498	G→A	A237A (GCG→GCA)	RPPX_12165 →	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	2,738,498	0	G	A	99.4%	340.9 / NA	173	A237A (GCG→GCA)	RPPX_12165	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (89/82); total (89/84)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

CTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGC  >  CP009974/2738355‑2738644

              tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                                                                               >  1:359946/8‑151 (MQ=1)

                gagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAg                                                                                                                             <  1:2083604/146‑1 (MQ=1)

                gagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTACCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGaa                                                                                                                             >  2:5660016/6‑150 (MQ=1)

                 agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc                                                                                                                            <  1:6709857/147‑2 (MQ=1)

                  gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                           >  1:2869055/4‑149 (MQ=1)

                  gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                           >  2:3047589/4‑149 (MQ=1)

                                                   ctgaCCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCt                                                                                          >  1:523479/4‑151 (MQ=0)

                                                            gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCg                                                                                 >  1:6030034/4‑151 (MQ=0)

                                                            gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCg                                                                                 <  2:1672564/148‑1 (MQ=1)

                                                            gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCg                                                                                 >  1:3695646/4‑151 (MQ=0)

                                                                  ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAtt                                                                           >  1:3317212/1‑150 (MQ=2)

                                                                  ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                           >  2:1717807/1‑151 (MQ=2)

                                                                  ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                           >  2:4213504/1‑151 (MQ=11)

                                                                  ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                           <  2:2477312/151‑1 (MQ=2)

                                                                  ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGGTTCTATTCCTTCGACCATg                                                                           >  1:2965556/1‑151 (MQ=11)

                                                                   gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                          <  2:1822900/151‑1 (MQ=2)

                                                                      ccGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGaca                                                                       >  1:2410558/1‑151 (MQ=2)

                                                                        gACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACAcc                                                                     >  1:4315278/1‑151 (MQ=2)

                                                                        gACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACAcc                                                                     >  2:2642154/1‑151 (MQ=11)

                                                                        gACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACAcc                                                                     >  2:1377682/1‑151 (MQ=11)

                                                                         aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                    <  2:1532646/151‑1 (MQ=2)

                                                                         aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                    <  1:5771364/151‑1 (MQ=2)

                                                                         aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                    <  1:1717807/151‑1 (MQ=2)

                                                                                 catgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctct                                                            <  2:2207156/145‑1 (MQ=11)

                                                                                  atgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCCCTg                                                           >  2:5110652/6‑151 (MQ=11)

                                                                                     ttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGAc                                                        >  2:1711175/3‑151 (MQ=11)

                                                                                      taGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTAGACt                                                       <  2:1624006/150‑1 (MQ=11)

                                                                                        gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                     >  1:1813370/1‑151 (MQ=14)

                                                                                        gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                     >  1:7056799/1‑151 (MQ=14)

                                                                                        gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                     >  1:7056382/1‑151 (MQ=14)

                                                                                           ccGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTg                                                  <  2:4838477/151‑1 (MQ=14)

                                                                                             gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                <  2:2694895/151‑1 (MQ=14)

                                                                                             gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                >  2:1282857/1‑151 (MQ=14)

                                                                                             gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                >  2:5405172/1‑151 (MQ=14)

                                                                                              tGCACTGGAACGACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGc                                               <  2:3313660/151‑1 (MQ=14)

                                                                                               gCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCg                                              <  2:911594/151‑1 (MQ=14)

                                                                                                 aCTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCGAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGaa                                            >  2:735129/1‑151 (MQ=14)

                                                                                                  cTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAc                                           >  1:1805145/1‑151 (MQ=17)

                                                                                                  cTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAc                                           <  1:976376/151‑1 (MQ=14)

                                                                                                    ggAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCACTGGACTCGGTGGCCGCAccc                                         >  2:6276987/1‑151 (MQ=14)

                                                                                                     ggaCTGCGCCGCCCCTCGGAACCAGCGCGAGCAGCGGGAGGCAGGCGACGAGATCAAGACCATGAGCGCCGGCTCTTCGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcccc                                        <  2:6573150/149‑1 (MQ=255)

                                                                                                     gAACGACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcccc                                        <  1:5971270/151‑1 (MQ=14)

                                                                                                      aaCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCg                                       >  2:5510010/1‑151 (MQ=14)

                                                                                                       aCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTCAGCAGGGTGGAGGTTTCGATTCCTTAGACCATGGACACCTGCTGCTCTGGGCGCGGTGGGCGAAccccaa                                      >  2:4338100/1‑149 (MQ=14)

                                                                                                        cTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAg                                     <  2:2439463/151‑1 (MQ=17)

                                                                                                         tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                    >  2:5456609/1‑151 (MQ=17)

                                                                                                         tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                    <  2:471/151‑1 (MQ=17)

                                                                                                         tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                    <  2:6376205/151‑1 (MQ=17)

                                                                                                         tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                    <  2:1073/151‑1 (MQ=17)

                                                                                                         tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                    >  1:6419306/1‑151 (MQ=17)

                                                                                                            gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                 <  2:1455815/148‑1 (MQ=17)

                                                                                                            gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                 <  1:6728311/148‑1 (MQ=17)

                                                                                                            gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                 <  2:5735507/148‑1 (MQ=17)

                                                                                                              cgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGGGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGc                               <  2:4743174/150‑1 (MQ=17)

                                                                                                               gCCACTCAGAGCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                              <  2:653442/151‑1 (MQ=17)

                                                                                                               gCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                              >  2:5006434/1‑151 (MQ=17)

                                                                                                               gCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                              >  1:4443579/1‑151 (MQ=17)

                                                                                                                ccACTCAGAACCAGCGCAAGCAGCGCGCGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGccc                             <  1:2462756/151‑1 (MQ=17)

                                                                                                                 cACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                            <  1:3363823/151‑1 (MQ=18)

                                                                                                                 cACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                            >  2:1299645/1‑151 (MQ=17)

                                                                                                                   ctcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGc                          >  1:7190507/3‑151 (MQ=17)

                                                                                                                    gcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCt                         <  2:5199194/150‑1 (MQ=18)

                                                                                                                     cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAACCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                        <  2:72308/151‑1 (MQ=18)

                                                                                                                     cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                        <  1:56952/151‑1 (MQ=18)

                                                                                                                     cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                        <  2:7084630/151‑1 (MQ=18)

                                                                                                                     cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                        <  1:7113515/151‑1 (MQ=18)

                                                                                                                      aGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGGGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTa                       >  2:5100796/1‑151 (MQ=17)

                                                                                                                        aaCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATg                     >  1:3272007/1‑151 (MQ=18)

                                                                                                                         aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                    >  1:4769679/1‑151 (MQ=18)

                                                                                                                         aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                    >  1:4056280/1‑151 (MQ=18)

                                                                                                                          ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                   >  1:3455832/1‑151 (MQ=18)

                                                                                                                          ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                   <  1:4667323/151‑1 (MQ=255)

                                                                                                                           cAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgc                  >  1:5073036/1‑151 (MQ=18)

                                                                                                                            aGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcg                 <  1:4759933/151‑1 (MQ=18)

                                                                                                                             gcgcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcgc                <  2:2007705/151‑1 (MQ=255)

                                                                                                                              cgcAAGCAGCGCGAGGCAGGCGACGAACTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCGCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc               <  1:2912464/151‑1 (MQ=255)

                                                                                                                              cgcAAGCAGCGCGAGGCAGGCGACGAAAGCAAAACCATGAGCGCCGGCGCTTGGGCTAGGCAGGGTGGGGGTTTCTATTCCTTCGACCATGGACGCCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGATCGcc               <  2:5962718/151‑1 (MQ=255)

                                                                                                                               gcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCg              >  1:6705299/1‑151 (MQ=18)

                                                                                                                               gcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACATGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcgctg              >  1:1558164/1‑149 (MQ=18)

                                                                                                                                cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGCCCATGGACACCTGCTGCTCTGGACTCGGGGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGACgg             >  2:3852188/1‑150 (MQ=255)

                                                                                                                                cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt             <  1:4999594/151‑1 (MQ=18)

                                                                                                                                 aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg            >  1:144763/1‑151 (MQ=255)

                                                                                                                                 aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg            >  1:144738/1‑151 (MQ=255)

                                                                                                                                 aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg            <  2:2080555/151‑1 (MQ=255)

                                                                                                                                  agcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGa           >  2:5880963/1‑151 (MQ=255)

                                                                                                                                   gcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAc          <  1:1397013/151‑1 (MQ=255)

                                                                                                                                    cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc         <  2:6636869/151‑1 (MQ=18)

                                                                                                                                    cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc         <  1:1929714/151‑1 (MQ=18)

                                                                                                                                    cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc         <  2:1719832/151‑1 (MQ=21)

                                                                                                                                    cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCGCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc         <  2:315575/151‑1 (MQ=18)

                                                                                                                                     ggcgcGAGGCGGGCGACGAAATCAGAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa        <  2:3246749/150‑1 (MQ=255)

                                                                                                                                     agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGCGCGCCGTGACCa        >  1:6881896/1‑151 (MQ=255)

                                                                                                                                     agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa        >  2:3759549/1‑151 (MQ=255)

                                                                                                                                     agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa        >  1:800493/1‑151 (MQ=255)

                                                                                                                                     agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa        <  1:7002734/151‑1 (MQ=255)

                                                                                                                                       cgcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAt      <  1:10098/151‑1 (MQ=255)

                                                                                                                                        gcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAtt     <  2:680383/151‑1 (MQ=21)

                                                                                                                                         cgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTg    <  1:1181329/151‑1 (MQ=255)

                                                                                                                                          gAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTgg   <  2:1007754/151‑1 (MQ=255)

                                                                                                                                          gAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTgg   >  1:5434409/1‑151 (MQ=255)

CTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGC  >  CP009974/2738355‑2738644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`