BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	2,738,513	G→A	K242K (AAG→AAA)	RPPX_12165 →	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	2,738,513	0	G	A	99.5%	456.2 / NA	206	K242K (AAG→AAA)	RPPX_12165	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (111/94); total (111/95)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

CGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAG  >  CP009974/2738369‑2738660

tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                                                                                               >  1:2906196/8‑151 (MQ=1)

  gagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTACCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGaa                                                                                                                                             >  2:5660016/6‑150 (MQ=1)

   agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc                                                                                                                                            <  1:6709857/147‑2 (MQ=1)

    gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                                           >  2:3047589/4‑149 (MQ=1)

                                              gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCg                                                                                                 >  1:3622923/4‑151 (MQ=0)

                                                   cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt                                                                                            >  2:3785492/1‑151 (MQ=2)

                                                   cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt                                                                                            <  2:3744259/151‑1 (MQ=11)

                                                    ggCGCTGACGGCTATCTTGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACTAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGTGCTAAGCAGGGTGGTGGTTTCTATTTCTTCTACCAtt                                                                                           >  2:1718050/1‑150 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAtt                                                                                           >  1:3317212/1‑150 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           >  2:4213504/1‑151 (MQ=11)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           <  2:7021841/151‑1 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                           <  2:2477312/151‑1 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGGTTCTATTCCTTCGACCATg                                                                                           >  1:2965556/1‑151 (MQ=11)

                                                        ccGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGaca                                                                                       >  1:2410558/1‑151 (MQ=2)

                                                         cGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGCTTCTATTCCTTCGACCATGGacac                                                                                      >  2:503022/1‑151 (MQ=2)

                                                          gACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACAcc                                                                                     >  2:1377682/1‑151 (MQ=11)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACAccg                                                                                    >  1:1985377/1‑150 (MQ=2)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                                    <  1:1718050/151‑1 (MQ=11)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                                    <  1:5771364/151‑1 (MQ=2)

                                                                   catgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctct                                                                            <  2:2207156/145‑1 (MQ=11)

                                                                    atgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCCCTg                                                                           >  2:5110652/6‑151 (MQ=11)

                                                                       ttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGAc                                                                        >  2:1711175/3‑151 (MQ=11)

                                                                        taGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACt                                                                       >  2:4928561/2‑151 (MQ=14)

                                                                          gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                                     >  1:1813370/1‑151 (MQ=14)

                                                                          gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                                     >  1:7056799/1‑151 (MQ=14)

                                                                          gCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCg                                                                     <  2:6355988/151‑1 (MQ=14)

                                                                           cTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTCAGCAGGGAGGTGGTCTCTATTCCTTCGACCATGGACACCTGCTGCTCTTGACGCgg                                                                    >  2:6621345/1‑151 (MQ=11)

                                                                            tCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGt                                                                   <  2:827210/151‑1 (MQ=14)

                                                                            tCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGt                                                                   <  1:4937029/151‑1 (MQ=14)

                                                                             ccGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTg                                                                  >  2:3283742/1‑151 (MQ=14)

                                                                               gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                                >  2:5405172/1‑151 (MQ=14)

                                                                               gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                                <  2:2694895/151‑1 (MQ=14)

                                                                                  cACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGa                                                             >  1:680383/1‑151 (MQ=14)

                                                                                      ggAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGGCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAccc                                                         <  2:4752381/151‑1 (MQ=14)

                                                                                       ggaCTGCGCCGCCCCTCGGAACCAGCGCGAGCAGCGGGAGGCAGGCGACGAGATCAAGACCATGAGCGCCGGCTCTTCGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcccc                                                        <  2:6573150/149‑1 (MQ=255)

                                                                                       gAACGACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcccc                                                        <  1:5971270/151‑1 (MQ=14)

                                                                                        aaCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCg                                                       >  2:5510010/1‑151 (MQ=14)

                                                                                         aCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTCAGCAGGGTGGAGGTTTCGATTCCTTAGACCATGGACACCTGCTGCTCTGGGCGCGGTGGGCGAAccccaa                                                      >  2:4338100/1‑149 (MQ=14)

                                                                                          cTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAg                                                     <  2:2439463/151‑1 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                                    >  1:6419306/1‑151 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                                    <  2:1073/151‑1 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                                    <  1:7007762/151‑1 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                                    <  2:471/151‑1 (MQ=17)

                                                                                            aCGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGa                                                   >  1:1521046/1‑151 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 <  2:1455815/148‑1 (MQ=17)

                                                                                              gccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATc                                                 <  2:5735507/148‑1 (MQ=17)

                                                                                               ccgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCg                                                >  2:1820455/3‑151 (MQ=17)

                                                                                                 gCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                                              >  1:4443579/1‑151 (MQ=17)

                                                                                                   cactcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                                            <  1:1360913/147‑1 (MQ=18)

                                                                                                   cACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                                            >  2:1299645/1‑151 (MQ=17)

                                                                                                   cACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCa                                            <  1:3363823/151‑1 (MQ=18)

                                                                                                     ctcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGc                                          >  1:7190507/3‑151 (MQ=17)

                                                                                                      gcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCt                                         <  2:5199194/150‑1 (MQ=18)

                                                                                                       cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                                        <  2:7084630/151‑1 (MQ=18)

                                                                                                       cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                                        <  1:56952/151‑1 (MQ=18)

                                                                                                       cAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCtt                                        >  2:1580416/1‑151 (MQ=18)

                                                                                                        aGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTa                                       >  2:6785363/1‑151 (MQ=18)

                                                                                                          aaCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATg                                     >  1:3272007/1‑151 (MQ=18)

                                                                                                           aCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGa                                    >  1:4056280/1‑151 (MQ=18)

                                                                                                            ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                                   <  1:4667323/151‑1 (MQ=255)

                                                                                                            ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                                   >  1:3455832/1‑151 (MQ=18)

                                                                                                             cAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgc                                  >  1:5073036/1‑151 (MQ=18)

                                                                                                              aGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcg                                 <  1:4759933/151‑1 (MQ=18)

                                                                                                                cgcAAGCAGCGCGAGGCAGGCGACGAACTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCGCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                               <  1:2912464/151‑1 (MQ=255)

                                                                                                                cgcAAGCAGCGCGAGGCAGGCGACGAAAGCAAAACCATGAGCGCCGGCGCTTGGGCTAGGCAGGGTGGGGGTTTCTATTCCTTCGACCATGGACGCCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGATCGcc                               <  2:5962718/151‑1 (MQ=255)

                                                                                                                 gcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCg                              >  1:6705299/1‑151 (MQ=18)

                                                                                                                  cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt                             <  1:4999594/151‑1 (MQ=18)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            >  2:185084/1‑151 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            <  2:2080555/151‑1 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            >  1:259376/1‑151 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                            >  1:144763/1‑151 (MQ=255)

                                                                                                                    agcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGa                           >  2:5880963/1‑151 (MQ=255)

                                                                                                                     gcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAc                          >  2:1632269/1‑151 (MQ=18)

                                                                                                                     gcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAc                          <  1:1397041/151‑1 (MQ=255)

                                                                                                                      cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc                         <  1:1929714/151‑1 (MQ=18)

                                                                                                                      cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCGCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc                         <  2:315575/151‑1 (MQ=18)

                                                                                                                       agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGCGCGCCGTGACCa                        >  1:6881896/1‑151 (MQ=255)

                                                                                                                       agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa                        >  1:800493/1‑151 (MQ=255)

                                                                                                                         cgcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAt                      <  1:10098/151‑1 (MQ=255)

                                                                                                                          gcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCCGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCACCTTATGAGCGCCGTGACCactc                     >  2:6423492/1‑148 (MQ=255)

                                                                                                                            gAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTgg                   <  2:1007754/151‑1 (MQ=255)

                                                                                                                            gAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTgg                   >  1:1624724/1‑151 (MQ=255)

                                                                                                                             aggcaggcGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGc                  >  1:5099731/1‑151 (MQ=21)

                                                                                                                              gtcaggggACGAAATCAAAACCATTAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCt                 <  1:5490048/144‑1 (MQ=21)

                                                                                                                              ggcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCt                 <  1:6973138/144‑1 (MQ=21)

                                                                                                                              ggcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGCTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCt                 >  1:2233752/8‑151 (MQ=255)

                                                                                                                               gcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTc                >  2:4983638/7‑151 (MQ=255)

                                                                                                                                 aggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCCTGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGCTCGCCCAGCTTATGCGCGCCGTGTCCAATTGGCTCaa              >  2:7242691/5‑151 (MQ=255)

                                                                                                                                 aggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCaa              >  2:7238549/5‑151 (MQ=21)

                                                                                                                                  ggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAg             >  2:169349/4‑151 (MQ=255)

                                                                                                                                   gcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGa            <  1:3296634/149‑1 (MQ=255)

                                                                                                                                   gcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGCGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGa            >  1:7169408/3‑151 (MQ=255)

                                                                                                                                      aCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAttt         >  2:6246268/1‑151 (MQ=255)

                                                                                                                                       cGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTc        >  1:3759308/1‑151 (MQ=255)

                                                                                                                                         aaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCgg      <  1:6031759/149‑1 (MQ=255)

                                                                                                                                         aaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCgg      <  1:426523/149‑1 (MQ=255)

                                                                                                                                          aaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGc     <  2:118376/150‑1 (MQ=25)

                                                                                                                                            tCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCa   >  1:1133660/1‑151 (MQ=255)

                                                                                                                                            tCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCa   >  1:3780260/1‑151 (MQ=255)

                                                                                                                                             cAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAg  <  2:3186072/151‑1 (MQ=255)

CGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAG  >  CP009974/2738369‑2738660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`