BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,358,424	2 bp→TC	intergenic (+285/+2404)	RPPX_05970 → / ← RPPX_05980	hypothetical protein/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,358,424	0	C	T	99.3%	775.1 / NA	300	intergenic (+285/+2405)	RPPX_05970/RPPX_05980	hypothetical protein/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (140/158); minor base G (0/2); total (140/160)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*	CP009974	1,358,425	0	T	C	99.3%	765.7 / NA	300	intergenic (+286/+2404)	RPPX_05970/RPPX_05980	hypothetical protein/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (140/158); minor base G (0/2); total (140/160)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

ATTGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCGGGACTGGCGGCTCCTTACGTCCGGGAGCAAGGGCATCTCATGATCTGGCCTCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTG  >  CP009974/1358280‑1358569

||

 ttGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAg                                                                                                                                            <  2:2812467/151‑1 (MQ=25)

  tGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGc                                                                                                                                           >  2:6810259/1‑151 (MQ=25)

  tGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGc                                                                                                                                           >  1:3177914/1‑151 (MQ=25)

  tGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGc                                                                                                                                           <  1:2406498/151‑1 (MQ=25)

       cAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGt                                                                                                                                      >  1:801172/1‑151 (MQ=21)

        aaTGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGtt                                                                                                                                     <  1:2835929/151‑1 (MQ=21)

         aTGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTa                                                                                                                                    <  1:6264548/151‑1 (MQ=21)

          tGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTAc                                                                                                                                   >  2:2484440/1‑151 (MQ=21)

              ttCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGt                                                                                                                               >  2:4714222/1‑151 (MQ=21)

              ttCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGt                                                                                                                               <  1:6030678/151‑1 (MQ=21)

                  tccCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGc                                                                                                                           <  1:5652445/151‑1 (MQ=21)

                    ccAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCgg                                                                                                                         >  2:1931575/1‑151 (MQ=21)

                       aGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTg                                                                                                                      <  2:6270184/151‑1 (MQ=18)

                        gATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTgg                                                                                                                     <  1:5315658/151‑1 (MQ=18)

                         aTCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGa                                                                                                                    <  1:6766222/151‑1 (MQ=18)

                          tCTAGTCCTAGCACAGACCTTATTTCGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAg                                                                                                                   <  1:4547262/151‑1 (MQ=18)

                          tCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAg                                                                                                                   >  1:654862/1‑151 (MQ=18)

                            tAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGc                                                                                                                 >  2:4383091/1‑151 (MQ=18)

                              gTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTg                                                                                                               <  1:3778284/151‑1 (MQ=18)

                                  tAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCAtt                                                                                                           >  2:5531923/1‑151 (MQ=17)

                                      acaGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCt                                                                                                       >  1:1745375/1‑151 (MQ=18)

                                        aGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGa                                                                                                     <  2:1714840/151‑1 (MQ=17)

                                        aGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGa                                                                                                     <  2:6286021/151‑1 (MQ=17)

                                        aGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGa                                                                                                     >  1:1114878/1‑151 (MQ=17)

                                          aCCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACg                                                                                                   >  1:3910074/1‑151 (MQ=17)

                                          aCCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACg                                                                                                   >  1:4478565/1‑151 (MQ=17)

                                          aCCTTATTTAGGGATCAAGGCACCTGCGACATGCGGGAAATGTCCTCTACGCTGGACTCGCGGCTCCTTACGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGCGCACCCCTACCGGCGGGCGGCTGGAGGCTGCAGCAGCTGACg                                                                                                   >  2:6050604/1‑151 (MQ=17)

                                           ccTTATTTAGGGATCAATGCACCTGCGACATGTGGGAAATTTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACga                                                                                                  <  1:2296856/151‑1 (MQ=17)

                                           ccTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACga                                                                                                  <  1:1866922/151‑1 (MQ=17)

                                            cTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACgag                                                                                                 <  2:2498787/151‑1 (MQ=17)

                                               aTTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGAcc                                                                                              >  1:5819879/1‑151 (MQ=17)

                                               aTTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGAcc                                                                                              >  1:3010786/1‑151 (MQ=17)

                                                tttAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCa                                                                                             <  1:2134279/151‑1 (MQ=17)

                                                  tAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGt                                                                                           <  2:3267572/151‑1 (MQ=17)

                                                  tAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGt                                                                                           <  1:7155670/151‑1 (MQ=17)

                                                  tAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGt                                                                                           <  2:3116210/151‑1 (MQ=17)

                                                  tAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGt                                                                                           <  1:2522096/151‑1 (MQ=17)

                                                   aGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTg                                                                                          >  2:92844/1‑151 (MQ=17)

                                                     ggATCACGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGcc                                                                                        <  2:7053061/151‑1 (MQ=14)

                                                     ggATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAgctag                                                                                        <  2:52402/151‑5 (MQ=17)

                                                         cAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCga                                                                                    >  2:2024658/1‑151 (MQ=255)

                                                          aaGGCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCgag                                                                                   >  1:3311586/1‑151 (MQ=17)

                                                             gCACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATc                                                                                >  2:6683236/1‑151 (MQ=17)

                                                              cACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATcc                                                                               >  1:2633897/1‑151 (MQ=17)

                                                              cACCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATcc                                                                               >  1:483222/1‑151 (MQ=17)

                                                               aCCTGCGGCATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGGATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCt                                                                              <  2:760914/151‑1 (MQ=14)

                                                               aCCTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCt                                                                              >  1:7019834/1‑151 (MQ=255)

                                                                ccTGCGACATGTGGGCAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTg                                                                             <  2:3752756/151‑1 (MQ=14)

                                                                 cTGCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGa                                                                            >  2:6439152/1‑151 (MQ=14)

                                                                   gCGACATGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGc                                                                          >  1:5989543/1‑151 (MQ=14)

                                                                        aTGTGGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGt                                                                     <  2:4447543/151‑1 (MQ=14)

                                                                         tgtgGGAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGtg                                                                    >  1:7207476/1‑151 (MQ=14)

                                                                            gggAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGtgcat                                                                 <  2:3657781/151‑1 (MQ=255)

                                                                              gAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGtgcatgc                                                               <  1:68077/151‑1 (MQ=14)

                                                                              gAAATGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGtgcatgc                                                               <  2:4599664/151‑1 (MQ=14)

                                                                               aaaTGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGCGCCGGGtgcatgca                                                              >  1:5203796/1‑151 (MQ=14)

                                                                               aaaTGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGtgcatgca                                                              <  1:2736773/151‑1 (MQ=14)

                                                                                aaTGTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCCTCTCGTGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGCGCCGCGAGATCCTCAGCCGGGtgcatgcac                                                             >  2:1177744/1‑150 (MQ=14)

                                                                                   gTCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCAc                                                          <  2:1274557/151‑1 (MQ=14)

                                                                                    tCCTCTACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACt                                                         >  2:2550553/1‑151 (MQ=14)

                                                                                      ctctACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGc                                                       <  1:4505566/151‑1 (MQ=17)

                                                                                      ctctACGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGc                                                       <  1:1914365/151‑1 (MQ=17)

                                                                                          aCGCTGGACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATg                                                   <  2:654862/151‑1 (MQ=14)

                                                                                            gctggAGTGGCGGCTCCTTAAGACCGAGAGCAAGGGAATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAc                                                 <  2:7203516/148‑1 (MQ=14)

                                                                                            gctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAc                                                 >  2:696740/4‑151 (MQ=14)

                                                                                            gctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAc                                                 <  2:4836367/148‑1 (MQ=255)

                                                                                            gctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAc                                                 >  1:4959225/4‑151 (MQ=14)

                                                                                             ctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAca                                                <  2:899376/149‑2 (MQ=14)

                                                                                             ctggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGGGCCGCGAGATCCTGAGCCGGGGGCAGGCAGCACTGCGATGAca                                                >  2:2095780/3‑150 (MQ=14)

                                                                                             ctggACTGGCGACTCCTACAGACCGGGAGCAAGGGCGTCTCATGATTTGGCTCCCAGAGCACCGGTACCGGTGGGCGGGTGGAGGCTGCGTTGGCTGACGAGACCAGTGCCGCGGGATCCTGAGCCGGATGCATGCAGCGCAGCGATGAca                                                >  2:6620896/3‑150 (MQ=14)

                                                                                              tggACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGAcag                                               <  1:6679894/150‑3 (MQ=14)

                                                                                                gACTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAggg                                             >  1:4072293/1‑151 (MQ=14)

                                                                                                  cTGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGc                                           >  1:3648337/1‑151 (MQ=14)

                                                                                                   tGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCa                                          <  2:3431978/151‑1 (MQ=14)

                                                                                                   tGGCGGCTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCa                                          >  2:4264290/1‑151 (MQ=14)

                                                                                                    ggcggcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCAt                                         <  2:6772543/151‑1 (MQ=14)

                                                                                                     gcggcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATg                                        <  2:1874789/151‑1 (MQ=14)

                                                                                                        gcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCt                                     <  2:2730189/151‑1 (MQ=14)

                                                                                                        gcTCCTTAAGACCGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCt                                     <  1:4382620/151‑1 (MQ=14)

                                                                                                              taagaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTgt                               <  2:1657608/146‑1 (MQ=255)

                                                                                                               aagaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTgtc                              <  1:4714222/147‑1 (MQ=255)

                                                                                                                 gaccGGGAGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTgtcag                            <  1:4199388/149‑1 (MQ=255)

                                                                                                                     gggAGCAAGGGCATCTCATGCTCTGGCTCCCTGAGCACCGTTACGGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGtcagtcag                        <  1:5064095/151‑1 (MQ=14)

                                                                                                                        aGCAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGt                      >  2:5382567/1‑150 (MQ=255)

                                                                                                                          cAAGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCa                   >  1:5263679/1‑151 (MQ=255)

                                                                                                                            aGGGCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGt                 >  1:1227758/1‑151 (MQ=255)

                                                                                                                              ggCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTcc               >  2:4931126/1‑151 (MQ=255)

                                                                                                                               gCATCTCATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCa              <  2:5914849/151‑1 (MQ=255)

                                                                                                                                   ctcATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTcc          >  2:4778656/1‑151 (MQ=255)

                                                                                                                                   ctcAGGAGCTGGCGCCCTGAGCACCGTTCCCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTcc          <  1:6296830/151‑1 (MQ=14)

                                                                                                                                    tcATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCt         >  2:4981427/1‑151 (MQ=255)

                                                                                                                                    tcAAGATCTGGCTCCCTGAGCACCGTTACCCGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCt         <  2:6824236/151‑1 (MQ=255)

                                                                                                                                     cctGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCtt        <  2:3732620/149‑1 (MQ=255)

                                                                                                                                     cATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCtt        >  1:3291000/1‑151 (MQ=255)

                                                                                                                                     cATGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCtt        >  1:1406035/1‑151 (MQ=255)

                                                                                                                                      aTGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTg                                    <  1:51052/122‑1 (MQ=255)

                                                                                                                                      aTGATCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTg       >  2:2328737/1‑151 (MQ=255)

                                                                                                                                         aTCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTc    <  1:148754/151‑1 (MQ=255)

                                                                                                                                         aTCTGGCTCCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGAATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTc    >  1:5445758/1‑151 (MQ=255)

                                                                                                                                           cTGGCTGCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACAGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTg  <  2:5609841/151‑1 (MQ=255)

||

ATTGAGCCAATGAGTTCCTCCCAAGATCTAGTCCTAGCACAGACCTTATTTAGGGATCAAGGCACCTGCGACATGTGGGAAATGTCCTCTACGCGGGACTGGCGGCTCCTTACGTCCGGGAGCAAGGGCATCTCATGATCTGGCCTCCTGAGCACCGTTACCGGTGGGCGGGTGGAGGCTGCATTGGCTGACGAGACCAGTGCCGCGAGATCCTGAGCCGGGTGCATGCAGCACTGCGATGACCGGGGCATGCCTGACTGTCAGTCAGGTGCAGTCCATTCCTTGGTCTG  >  CP009974/1358280‑1358569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`