Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,897,027	4 bp→GACC	intergenic (‑4805/‑3789)	RPPX_08470 ← / → RPPX_08480	tryptophan synthase subunit beta/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,897,027	0	C	G	100.0%	12.6 / NA	12	intergenic (‑4805/‑3792)	RPPX_08470/RPPX_08480	tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/3);  total (9/3)
*	CP009974	1,897,028	0	G	A	100.0%	12.3 / NA	12	intergenic (‑4806/‑3791)	RPPX_08470/RPPX_08480	tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/3);  total (9/3)
*	CP009974	1,897,029	0	T	C	100.0%	12.6 / NA	12	intergenic (‑4807/‑3790)	RPPX_08470/RPPX_08480	tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/3);  total (9/3)
*	CP009974	1,897,030	0	G	C	100.0%	12.6 / NA	12	intergenic (‑4808/‑3789)	RPPX_08470/RPPX_08480	tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (9/3);  total (9/3)

TCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATCGTGGTCTGA‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAACTTGCCAACGTCC  >  CP009974/1896923‑1897081                                                                                                         ||||                                                     tCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGc          >  1:5289578/1‑151 (MQ=11)  ccAGCGCCTGGCTCACTTTCACGGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGcc         >  1:4154857/1‑151 (MQ=11)   cAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCCGTTGCTCGAAGTTGccg        >  2:6493642/1‑150 (MQ=11)    aGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc       >  2:4818064/1‑149 (MQ=11)    aGCGCCTGGCTCACCTTCACAGTAAAGGAAGGTTGCTGGTCTGCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc       <  2:6805679/151‑3 (MQ=11)      cgcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGcggccc     >  1:2921107/1‑146 (MQ=11)      cgcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgccc     >  2:3696975/1‑147 (MQ=1)       gcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccg    <  1:3520682/151‑6 (MQ=11)        ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt   >  1:259545/1‑145 (MQ=11)        ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt   <  1:6436964/151‑7 (MQ=11)        ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt   >  1:6843030/1‑145 (MQ=11)         cTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgtg  >  2:6072922/1‑144 (MQ=11)                                                                                                         ||||                                                     TCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATCGTGGTCTGA‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAACTTGCCAACGTCC  >  CP009974/1896923‑1897081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑