Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,897,916 2 bp→CT intergenic (‑5694/‑2902) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,897,9160AC93.3% 13.8 / ‑4.8 15intergenic (‑5694/‑2903)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (7/7);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0099741,897,9170CT86.7% 12.6 / ‑6.6 15intergenic (‑5695/‑2902)RPPX_08470/RPPX_08480tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (6/7);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCGTACACCCACGACGCACAGGGCGATGACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGTGGTGCTGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGGGTGGC  >  CP009974/1897766‑1897971
                                                                                                                                                      ||                                                      
gcgcCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTTGGTATCAATTCGGCGGTAGACGCCACCGGCGCTACGTTCGAGAACCTGCAACTGGGCGGTGCGGCTACTGTTCAAGTGACCGAcacca                                                         >  1:6040580/1‑151 (MQ=2)
                       aggCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTTGGTATCAATTCGGCGGTAGACGCCACCGGCGCAACGTTCGAGAACCTGCAGTTGGGCGGTGCGGCTACTGTTCAAGAGACCGACACCACCGACGAAGTAGTGGCCAAGCtc                                  >  2:4916562/2‑150 (MQ=255)
                                 gTCTACCAGGACGCTGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGAccc                        >  1:4564504/1‑151 (MQ=1)
                                  tCTACCAGGACGCTGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCCACACCCCCGACGAAGTGATGGCCAAGCTGACAGCGAcccc                       >  2:4512681/1‑151 (MQ=11)
                                               tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa          <  2:3341653/150‑1 (MQ=11)
                                               tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa          <  2:3346575/150‑1 (MQ=11)
                                                ggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAg         <  1:4761499/151‑1 (MQ=11)
                                                 gCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAgg        >  1:73464/1‑151 (MQ=11)
                                                  ccAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggc       >  2:6060978/1‑150 (MQ=11)
                                                    aGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg     >  2:30621/1‑148 (MQ=11)
                                                    aGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCATCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg     <  2:5280497/151‑4 (MQ=11)
                                                     gATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggg    >  2:1545937/1‑147 (MQ=11)
                                                      aTCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtg   <  1:3603649/151‑6 (MQ=11)
                                                      aTCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtg   <  1:3964651/151‑6 (MQ=11)
                                                      aTCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtg   >  2:3710120/1‑146 (MQ=11)
                                                       tCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtga  <  1:5992471/151‑7 (MQ=11)
                                                                                                                                                      ||                                                      
GCGCCGTACACCCACGACGCACAGGGCGATGACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGTGGTGCTGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGGGTGGC  >  CP009974/1897766‑1897971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑