Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	418,449	Δ1 bp	intergenic (‑28/+623)	RPPX_01715 ← / ← RPPX_01720	hypothetical protein/lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	418,449	0	T	.	100.0%	53.6 / NA	34	intergenic (‑28/+623)	RPPX_01715/RPPX_01720	hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/23);  total (11/23)

TTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGG  >  CP009974/418303‑418478                                                                                                                                                   |                               ttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatgg                          >  2:260869/1‑151 (MQ=14) ttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatgg                          >  2:260849/1‑151 (MQ=14) ttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatgg                          >  2:260834/1‑151 (MQ=14) ttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatgg                          <  1:3277761/151‑1 (MQ=14)  tgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATggg                         >  2:4660871/1‑151 (MQ=11)   gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                        <  2:1974672/151‑1 (MQ=11)    ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                       >  2:4523666/1‑151 (MQ=11)     tgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc                      <  1:91051/151‑1 (MQ=11)      gttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt                     >  2:2292990/1‑151 (MQ=11)       ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                    <  1:2527417/151‑1 (MQ=11)       ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                    <  2:2051043/151‑1 (MQ=11)       gtgctgttggtgttgctgttgctgttgATCTAGATCTCGGTCTTGATCTTGATCTTGATCTTCGCGACTTCACGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGTATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                    <  2:4262427/150‑1 (MQ=11)       gtgctggtgctgttgctgttgctgttgGTCGTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTGGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                    <  2:3630235/150‑1 (MQ=11)        tgctgttgctgttgctgttgctgttgATCTTGAGCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGc                   <  2:1991806/151‑1 (MQ=11)         gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATGTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTATGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtcag                  <  1:4891633/151‑4 (MQ=11)         gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                  <  1:5276325/151‑1 (MQ=14)         gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                  <  2:4852304/151‑1 (MQ=11)         gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                  >  1:1887957/1‑151 (MQ=14)          ctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtcagc                 <  1:552025/151‑5 (MQ=11)           tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                >  1:3821404/1‑151 (MQ=14)           tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                >  2:77780/1‑151 (MQ=14)            gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg               <  2:1475727/151‑1 (MQ=14)             ttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcgc              <  1:5073235/151‑1 (MQ=14)               gctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑gg           <  1:1911090/151‑2 (MQ=14)                 tgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtc         <  1:587746/151‑4 (MQ=11)                   ttgctgttgctgttgATCTTGCTCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGCGGTGCCCGGCCTGGATGCCCGGCACGCGCCGCGG‑CCCA‑GGGTGCGGCCTGCAGcgc‑gggtcc       >  2:4681727/1‑146 (MQ=11)                   ttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcct       <  1:4362213/151‑6 (MQ=11)                   ttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcct       <  1:4110291/151‑6 (MQ=11)                    tgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctn      <  1:1816945/151‑7 (MQ=11)                    tgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctc      >  2:4080/1‑145 (MQ=11)                    tgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctc      <  2:5155990/151‑7 (MQ=11)                     gctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctcc     <  1:4916041/151‑8 (MQ=11)                       tgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCg    >  2:4443127/1‑151 (MQ=11)                        gttgctgttgATCTTGATCTTGGTCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg   <  2:190363/151‑1 (MQ=1)                        gttgctgttgATCTTGATCTTGCTCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAGCGTAGGCCTTGCGGAGGGAGGTGACGGGCGTGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg   <  2:1786869/151‑1 (MQ=11)                        gttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg   <  1:1171336/151‑1 (MQ=11)                         ttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCTGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCggg  >  1:2766821/1‑151 (MQ=11)                         ttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCggg  >  1:4380285/1‑151 (MQ=11)                                                                                                                                                   |                               TTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGG  >  CP009974/418303‑418478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑