| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009974 | 4,031,017 | Δ1 bp | intergenic (+159/‑625) | RPPX_17905 → / → RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009974 | 4,031,017 | 0 | A | . | 91.7% | 109.3 / 8.2 | 36 | intergenic (+159/‑625) | RPPX_17905/RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base . (17/16); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCA‑GGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGG > CP009974/4030871‑4031167 | gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc > 1:3106084/1‑151 (MQ=255)gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc > 1:3681641/1‑151 (MQ=16)gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc > 2:270408/1‑151 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttctgcTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc < 1:697406/151‑1 (MQ=255) ttctgcttctgcttctgcttctgcttctgcttctgcTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc < 1:852286/151‑1 (MQ=255) tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa < 2:5059353/151‑1 (MQ=16) ttgcttcggattctgcttctgcttctgcttctgcttTTGCTTCTCAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑g < 1:2073309/150‑1 (MQ=11) tgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGCCGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑gg > 2:2142805/1‑151 (MQ=255) gcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGa > 1:1763211/1‑151 (MQ=16) gctgctgcttctgcttctgcttctgcttctgcttTTGCTTCTCAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGACGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGa < 2:3481242/151‑1 (MQ=11) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATg > 2:1052902/1‑151 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATg < 2:3049066/151‑1 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATg < 1:5125066/151‑1 (MQ=16) ctgcttctgcttctgcttctgcttctgcttTTGCTTCTAGGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATggg < 1:5295473/151‑1 (MQ=255) ctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATggg > 1:323683/1‑151 (MQ=255) ctgcttctgcttcggcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATggg < 1:3858899/151‑1 (MQ=255) gcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc < 2:1763211/151‑1 (MQ=16) cttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc > 2:317539/1‑151 (MQ=255) cttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc > 2:587619/1‑151 (MQ=12) ttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt < 2:714098/151‑1 (MQ=16) ttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt > 1:1211761/1‑151 (MQ=255) ttcggcttctgcttcggcttctgcttTTGCGTCAACGCGCGCGATGGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGCGCGCAGGGCTGGCGAAGGGGGGTGACGGGCTTTGATGCCCGTCACGCGCTGAGGACCCATGGATGGGGc < 2:2858868/151‑1 (MQ=11) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtg > 2:3273285/1‑150 (MQ=16) tctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtg > 1:1182011/1‑150 (MQ=255) tgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGCGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgca < 1:4093380/151‑4 (MQ=255) tgcgtctgcttctggttctgcttGTGCTTCTAGGGACGCGATAGGTGGGGCGACTCAGTTTGCTGCTTGAGGAGGCGGAGTGCAGGGCTTGTGAAGGGAGGTGACGTGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgca < 2:4349027/151‑4 (MQ=2) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgcagc < 2:3074794/151‑6 (MQ=2) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgcagc > 1:3338203/1‑146 (MQ=255) ttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgcagcg < 1:3939573/151‑7 (MQ=255) ttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgcagcg > 2:346277/1‑145 (MQ=255) ttctgctgcggcttcggcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCtgcagcg < 2:559177/151‑7 (MQ=1) tgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCgg > 1:4216857/1‑151 (MQ=255) gcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGt > 1:1951132/1‑151 (MQ=255) tctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCt > 1:399776/1‑151 (MQ=255) gAGGCCCCA‑GGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAg < 1:850631/151‑1 (MQ=11) gAGGCCCCA‑GGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAg > 2:4130074/1‑151 (MQ=11) aGGCCGGA‑GGATGGGGCGTGCGGGGCGGTCCTGCCGGGAGGAAGGCCGGAGTGAGGGGACGCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAgg < 2:3162067/151‑1 (MQ=11) | GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCA‑GGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGG > CP009974/4030871‑4031167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |