Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,631,689	Δ1 bp	intergenic (+251/‑579)	emrB → / → RPPX_20645	multidrug resistance protein B/oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,631,689	0	G	.	100.0%	154.9 / NA	40	intergenic (+251/‑579)	emrB/RPPX_20645	multidrug resistance protein B/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/26);  total (14/26)

GCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631543‑4631712                                                                                                                                                   |                        gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  1:4834815/151‑1 (MQ=255) gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  1:4834647/151‑1 (MQ=255) gCGTAGGGCTTGCGAAGGGAGGTGGCGGGCATGGAAGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  2:2420677/151‑1 (MQ=255)  cgcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCgc                   >  1:5208981/4‑150 (MQ=255)  cgcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   >  1:1558017/4‑151 (MQ=255)  cGTAGGGCTTGCGAAGGGGGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGACTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   <  1:1585364/151‑1 (MQ=255)  cGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   >  1:693846/1‑151 (MQ=255)   gcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  2:601439/149‑1 (MQ=255)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGGTGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCGTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  2:3452462/151‑1 (MQ=255)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  1:5432623/151‑1 (MQ=255)    tggggCTTGCGAAGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCGGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 <  2:2266927/149‑1 (MQ=11)    tAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 <  2:2804909/151‑1 (MQ=255)     ggggCTGGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:1816461/150‑1 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                >  1:1306851/1‑151 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:1070480/151‑1 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGgttt                >  1:3692283/1‑151 (MQ=255)      gggCTTGCGAGGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  2:2206600/151‑1 (MQ=255)       ggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttgttt              >  2:3312452/1‑151 (MQ=255)        gCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg             >  1:3373748/1‑151 (MQ=255)        gCTTGCGAAGGGAGGGCGCGGGTATGGAGGCCCGTCAAGCGCTGAGGCCGTATGGATGGGGCCTGCAGCGCGTCTGCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg             <  2:2908345/151‑1 (MQ=11)          ttGCGAAGGGAGGTGACGGGCAGGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  1:3801612/151‑1 (MQ=255)          ttGCGAAGGGAGGGGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  2:1997409/151‑1 (MQ=255)           tGCGAAGGGGGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCGGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  1:5364695/151‑1 (MQ=255)           tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  1:1401007/151‑1 (MQ=255)           tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          >  1:2044138/1‑151 (MQ=255)           tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  2:5331421/151‑1 (MQ=16)           ggCGAGGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  2:5332567/150‑1 (MQ=11)            gggaggggAGGTGGCGGGCAGGGAGGCCCGTCAAGCGCGGAGGACAGATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  2:2402208/146‑1 (MQ=11)            gCGAAGGGAGGTGGCGGGGATGGGTGCCCGTCAAGCGCTGAGGCCGCATGGTGGGGGCCTGAAGCGCGTACTCACGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  2:1963037/151‑1 (MQ=11)            gCGAAGGGAGGTGACGTGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  1:4201621/151‑1 (MQ=255)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  2:3013412/151‑1 (MQ=255)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  2:1768076/1‑151 (MQ=16)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:4318364/1‑151 (MQ=16)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:4316626/1‑151 (MQ=16)               aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:4320790/151‑1 (MQ=12)                aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     <  2:1207079/151‑1 (MQ=9)                aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     >  1:645074/1‑151 (MQ=9)                aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     >  1:4948137/1‑151 (MQ=9)                  ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   >  1:1271147/1‑151 (MQ=9)                   gAGGTGACGGGCATGGAGGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  2:4788604/151‑1 (MQ=2)                                                                                                                                                   |                        GCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631543‑4631712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑