BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	2,738,537	C→T	A250A (GCC→GCT)	RPPX_12165 →	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	2,738,537	0	C	T	99.4%	475.3 / ‑5.2	180	A250A (GCC→GCT)	RPPX_12165	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (86/93); total (86/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 180 total aligned reads displayed.

AGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGATTCAG  >  CP009974/2738393‑2738684

 ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                                                                                                                              >  2:1779345/4‑151 (MQ=0)

 ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                                                                                                                              >  2:324255/4‑151 (MQ=0)

 ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                                                                                                                              >  2:4119968/4‑151 (MQ=0)

                      gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCg                                                                                                                         >  2:2858554/4‑151 (MQ=0)

                           cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt                                                                                                                    >  1:2642600/1‑151 (MQ=2)

                            ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                                                                   <  1:3496694/151‑1 (MQ=2)

                             gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                                                                  <  2:3370886/151‑1 (MQ=2)

                             gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                                                                  >  1:5226025/1‑151 (MQ=2)

                               gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac                                                                                                                >  2:3806900/1‑151 (MQ=2)

                                   aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                                                            >  2:2646067/1‑151 (MQ=2)

                                   aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                                                            >  1:2596495/1‑151 (MQ=2)

                                   aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                                                            >  2:668703/1‑151 (MQ=2)

                                   aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                                                            <  2:4236934/151‑1 (MQ=11)

                                   aCGGCTATCATGTTAGCGCGGTGCACTGGAACTACGCCGCTAATCGCAACCAGCGCAAGCAGCGCGAGGCAGGCGACTAAGTTAAATCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTTGTTGTTTCTATTCCTTCGAGCATGGACTCct                                                                                                            <  2:3041600/151‑1 (MQ=2)

                                    cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg                                                                                                           <  2:2365483/151‑1 (MQ=2)

                                    cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg                                                                                                           >  1:2506923/1‑151 (MQ=11)

                                      gCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctgct                                                                                                         >  1:4515264/1‑151 (MQ=11)

                                          tcatgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctc                                                                                                     <  1:4858875/144‑1 (MQ=11)

                                           catgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctct                                                                                                    <  1:1415458/145‑1 (MQ=2)

                                            atgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTg                                                                                                   >  1:220555/6‑151 (MQ=11)

                                             tgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTgg                                                                                                  <  1:3256087/147‑1 (MQ=11)

                                                   cTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCgg                                                                                            >  2:1838182/1‑151 (MQ=14)

                                                     ccGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTg                                                                                          <  1:2099453/151‑1 (MQ=14)

                                                      cGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTgg                                                                                         <  1:105033/151‑1 (MQ=14)

                                                      cGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTgg                                                                                         <  1:104673/151‑1 (MQ=14)

                                                      cGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTgg                                                                                         >  1:4538964/1‑151 (MQ=14)

                                                       gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                                                        <  1:809449/151‑1 (MQ=14)

                                                             tGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcc                                                                                  >  2:321968/1‑151 (MQ=14)

                                                               gAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcccc                                                                                <  2:1385394/151‑1 (MQ=17)

                                                                  cTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAg                                                                             <  2:219614/151‑1 (MQ=17)

                                                                  cTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAg                                                                             >  2:3220652/1‑151 (MQ=17)

                                                                   tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                                                            <  1:964545/151‑1 (MQ=17)

                                                                   tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                                                            >  2:3905787/1‑151 (MQ=17)

                                                                    aCGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAGCCCCGAGGa                                                                           >  2:1298712/1‑151 (MQ=17)

                                                                     cgccgcCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGAt                                                                          <  1:4949585/151‑1 (MQ=17)

                                                                        cgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTTCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAggtttgc                                                                       >  1:408337/2‑146 (MQ=17)

                                                                        cgCCACTCAGAACCAACGCAAGCAGCGCGAGGCAGGCGACGAAAGCAAAACCAGGAGCGCCGGCTCTTAGGCTAAGCAGGGTGGTGGTGTCTATTCCTTCGACCAGGGACGCCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGc                                                                       <  2:3620336/150‑1 (MQ=255)

                                                                          ccACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGccc                                                                     >  1:786268/1‑151 (MQ=17)

                                                                            actcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAg                                                                   >  2:1165595/4‑151 (MQ=17)

                                                                              tcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCt                                                                 <  2:1887958/150‑1 (MQ=18)

                                                                                  aaCCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATg                                                             >  1:425207/1‑151 (MQ=18)

                                                                                    ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                                                           >  1:4701486/1‑151 (MQ=18)

                                                                                      aGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcg                                                         <  2:2573089/151‑1 (MQ=18)

                                                                                        cgcAAGCAGCGCGAGGCAGGCGACGAAATCAACACCAGGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       <  2:2752582/151‑1 (MQ=255)

                                                                                        cgcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       <  2:834715/151‑1 (MQ=255)

                                                                                        cgcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       <  2:4735549/151‑1 (MQ=255)

                                                                                        cgcAAGCAGCGCGAGCCAGGCGACGACATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGAGCGCCCAGCTTATGAGCGcc                                                       <  2:3538825/151‑1 (MQ=255)

                                                                                          cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt                                                     >  1:4221109/1‑151 (MQ=255)

                                                                                          cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt                                                     >  2:4508740/1‑151 (MQ=255)

                                                                                           aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg                                                    >  1:772281/1‑151 (MQ=255)

                                                                                            agcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGa                                                   >  2:1731813/1‑151 (MQ=255)

                                                                                              cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc                                                 <  1:5143558/151‑1 (MQ=18)

                                                                                              cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGCATCGCCCAGCTTATGAGCGCCGTGAcc                                                 >  2:2738838/1‑151 (MQ=255)

                                                                                               agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa                                                >  2:5027670/1‑151 (MQ=255)

                                                                                                gcgcGAGGCAGGCGACGCAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCCCTGGACTCGGTGGGCGACCTCCGAGGATCGCCCCGCTTATTAGCGCCGTGACCac                                               >  2:2698408/1‑150 (MQ=255)

                                                                                                gcgcGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCaa                                               >  1:5195923/1‑151 (MQ=255)

                                                                                                 cgcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAt                                              >  2:2961693/1‑151 (MQ=18)

                                                                                                  gcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAtt                                             >  2:1685961/1‑151 (MQ=255)

                                                                                                   cgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTg                                            >  2:2850142/1‑151 (MQ=255)

                                                                                                     aggcaggcGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGc                                          <  1:144461/151‑1 (MQ=255)

                                                                                                      ggcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCt                                         <  2:2036425/144‑1 (MQ=21)

                                                                                                       gcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTc                                        <  2:200642/145‑1 (MQ=255)

                                                                                                       gcaggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTc                                        >  2:3271331/7‑151 (MQ=255)

                                                                                                        caggcgACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCa                                       <  1:727060/146‑1 (MQ=255)

                                                                                                             gACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAtt                                  >  1:3778863/1‑151 (MQ=21)

                                                                                                              aCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAttt                                 >  1:4149203/1‑151 (MQ=255)

                                                                                                              aCGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGAttt                                 <  2:5226025/151‑1 (MQ=25)

                                                                                                               cGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTc                                >  1:1345927/1‑151 (MQ=255)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       <  2:4462035/124‑1 (MQ=255)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       <  2:4462108/124‑1 (MQ=255)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       >  1:4462035/4‑127 (MQ=21)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                                                       >  1:4462108/4‑127 (MQ=21)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCg                               <  2:2642600/148‑1 (MQ=255)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCg                               <  1:4362098/148‑1 (MQ=255)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCg                               <  1:3350667/148‑1 (MQ=255)

                                                                                                                gaaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGAGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTCGGCGATCCCCGAGGACCGCGCAGCTTATGAGCGCCGTGCCCACTTGGCTCACGATTTCg                               >  2:4632489/4‑151 (MQ=255)

                                                                                                                 aaaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCgg                              <  1:4196428/149‑1 (MQ=255)

                                                                                                                  aaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGc                             >  2:5044641/2‑151 (MQ=255)

                                                                                                                  aaTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGc                             <  2:3967119/150‑1 (MQ=25)

                                                                                                                   aTCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGcc                            >  1:202987/1‑151 (MQ=255)

                                                                                                                    tCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCa                           <  2:2311334/151‑1 (MQ=255)

                                                                                                                     cAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAg                          <  1:1232330/151‑1 (MQ=255)

                                                                                                                      aaaaCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAgg                         <  1:2927645/148‑1 (MQ=25)

                                                                                                                         aCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGccc                      >  1:2447697/1‑151 (MQ=21)

                                                                                                                         aCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGccc                      >  1:158434/1‑151 (MQ=37)

                                                                                                                          ccATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCcg                     >  1:3870207/1‑151 (MQ=255)

                                                                                                                           cATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCcgc                    <  2:1935188/151‑1 (MQ=255)

                                                                                                                                   gcggCGCTTGGGGTAAGCAGGGTGGTGGTTTCTATGCCGTCGTCCTTGGACACCTGCTGCTCTGGTCTCGGTGGGCGAGCCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCCATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTg            <  2:3988999/149‑1 (MQ=255)

                                                                                                                                    cggctcttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTgg           >  1:3706510/8‑151 (MQ=255)

                                                                                                                                     ggctcttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGga          >  2:3767830/7‑151 (MQ=37)

                                                                                                                                       ctcttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGCCTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGgatg        <  2:2506923/147‑1 (MQ=255)

                                                                                                                                        tcttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGgatga       <  1:4445847/148‑1 (MQ=255)

                                                                                                                                         cttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGgatgat      >  2:2605975/3‑151 (MQ=255)

                                                                                                                                          ttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGAtt     <  1:3965378/150‑1 (MQ=255)

                                                                                                                                          ttGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGAtt     >  1:3549972/2‑151 (MQ=255)

                                                                                                                                           tGGGCTAAGCAGGCTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGATTc    <  1:5234826/151‑1 (MQ=255)

                                                                                                                                            gggCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGACCGCGCAGACTGGATGATTCa   >  2:4838876/1‑151 (MQ=255)

                                                                                                                                             ggCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGATTCAg  >  1:5424587/1‑151 (MQ=255)

                                                                                                                                             ggCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGATTCAg  <  1:627175/151‑1 (MQ=255)

                                                                                                                                             ggCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGATTCAg  <  1:3053862/151‑1 (MQ=255)

AGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCTCAAGATTTCGGCCAGGCCCGCGCAGACTGGATGATTCAG  >  CP009974/2738393‑2738684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`