Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 3,971,951 G→T S9S (TCC→TCA)  RPPX_17675 ← hemolysin D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099743,971,9510GT97.3% 869.6 / NA 268S9S (TCC→TCA) RPPX_17675hemolysin D
Reads supporting (aligned to +/- strand):  ref base G (7/0);  new base T (139/117);  total (151/117)
Fisher's exact test for biased strand distribution p-value = 1.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098
                                                                                                                                                      |                                                                                                                                                   
   ggCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAg                                                                                                                                                  <  1:4326984/151‑1 (MQ=255)
    gCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAgg                                                                                                                                                 >  1:5049531/1‑151 (MQ=255)
       tcgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGtt                                                                                                                                              >  2:5354217/1‑151 (MQ=255)
        cgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGTCTAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGttt                                                                                                                                             <  1:2269016/151‑1 (MQ=255)
        cgtcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGttt                                                                                                                                             >  2:3613507/1‑151 (MQ=255)
          gcgGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGt                                                                                                                                           <  1:972253/150‑1 (MQ=255)
             gtggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGGGGGTTTGTCTa                                                                                                                                        >  2:4928329/1‑151 (MQ=255)
              tggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       <  1:1642028/151‑1 (MQ=255)
              tggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       <  1:4232095/151‑1 (MQ=255)
              tggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       <  1:1147758/151‑1 (MQ=255)
              tggtGTGCAGGTGGCGGCGGGTGCTGGAGCGGTAGCGGGTGAAGGCCGGCAGGGTGAGTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTAc                                                                                                                                       <  2:4737871/151‑1 (MQ=255)
               ggtTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACg                                                                                                                                      <  1:2414298/151‑1 (MQ=255)
                 tttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGc                                                                                                                                    >  1:1748695/1‑151 (MQ=255)
                  ttCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCa                                                                                                                                   >  2:2485952/1‑151 (MQ=255)
                    ccAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAAc                                                                                                                                 <  2:4168588/151‑1 (MQ=255)
                    ccAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAAc                                                                                                                                 <  2:143355/151‑1 (MQ=255)
                      aGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAt                                                                                                                               >  1:4385890/1‑151 (MQ=255)
                      aGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACAt                                                                                                                               >  2:92594/1‑151 (MQ=255)
                       gATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACAtt                                                                                                                              >  2:2892372/1‑151 (MQ=255)
                           gCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtg                                                                                                                          >  1:1105765/1‑151 (MQ=255)
                             ggCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGTTGATTTCCAGGCGGCGACGGGGTTTAGCGCTCGGCCGCGGTTCAGGTTCGGCGGGTGAGGGTTTGTCTACGGCATCGTTCAtggg                                                                                                                        >  2:2017167/1‑150 (MQ=255)
                             ggCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggt                                                                                                                        >  1:2924014/1‑151 (MQ=255)
                             ggCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGCTTGTCTACGGCAACATTCAgggt                                                                                                                        >  2:3856025/1‑151 (MQ=255)
                               ccGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCAtggttg                                                                                                                      >  1:3851741/1‑151 (MQ=255)
                                 ggTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggttggt                                                                                                                    >  1:769685/1‑151 (MQ=255)
                                 ggTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCAtggttggt                                                                                                                    >  1:4962050/1‑151 (MQ=255)
                                   tGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGttt                                                                                                                  <  1:4103860/151‑1 (MQ=255)
                                       gAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCAt                                                                                                              <  2:1357476/151‑1 (MQ=255)
                                          ccAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtct                                                                                                           <  2:2400791/151‑1 (MQ=255)
                                          ccAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtct                                                                                                           <  1:5171421/151‑1 (MQ=255)
                                          ccAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtct                                                                                                           >  2:710178/1‑151 (MQ=255)
                                          ccAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGCGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATtct                                                                                                           >  1:5236586/1‑151 (MQ=255)
                                               aGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAAt                                                                                                      <  1:53508/151‑1 (MQ=255)
                                                     tGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGcc                                                                                                >  2:1720278/1‑151 (MQ=255)
                                                     tGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGcc                                                                                                >  1:204172/1‑151 (MQ=255)
                                                      gAAGGCCAGGAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGTGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCct                                                                                               <  2:2016118/151‑1 (MQ=255)
                                                      gAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCct                                                                                               <  2:2250774/151‑1 (MQ=255)
                                                       aaGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctg                                                                                              <  2:3087301/151‑1 (MQ=255)
                                                       aaGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctg                                                                                              <  1:2616536/151‑1 (MQ=255)
                                                       aaGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctg                                                                                              <  1:1718943/151‑1 (MQ=255)
                                                        aGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgc                                                                                             >  2:3961650/1‑151 (MQ=255)
                                                          gCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCctgctg                                                                                           <  1:3613507/151‑1 (MQ=255)
                                                              gaagagTGATTGCGGCCAGGCTGCCGATGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTgctggctg                                                                                       <  1:406461/146‑1 (MQ=255)
                                                                agGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTc                                                                                     >  2:1281606/1‑151 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCg                                                                                    <  1:1379477/151‑1 (MQ=255)
                                                                 gggTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCg                                                                                    <  1:584784/151‑1 (MQ=255)
                                                                   gTGATTGCGGCCAGGCTGGCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGt                                                                                  <  2:2628339/151‑1 (MQ=255)
                                                                   gTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGt                                                                                  >  1:189946/1‑151 (MQ=255)
                                                                   gTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGt                                                                                  <  2:1308498/151‑1 (MQ=255)
                                                                     gATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATGCATGGTTGGTTTCCATTCTCGACTTGGGCCTGCTGGCTGTCGGTtg                                                                                >  2:3354022/1‑151 (MQ=255)
                                                                        tGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTgg                                                                             <  2:2264414/151‑1 (MQ=255)
                                                                        tGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTgg                                                                             <  2:581103/151‑1 (MQ=255)
                                                                        tGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTgg                                                                             >  1:482089/1‑151 (MQ=255)
                                                                         gCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTggg                                                                            >  2:2500667/1‑151 (MQ=255)
                                                                         gCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTggg                                                                            >  1:2822847/1‑151 (MQ=255)
                                                                           ggCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAg                                                                          >  2:3774972/1‑151 (MQ=255)
                                                                           ggCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAg                                                                          >  1:3096349/1‑151 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      >  2:3286596/1‑151 (MQ=255)
                                                                               aGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGCTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCAc                                                                      >  1:4614166/1‑151 (MQ=255)
                                                                                 gCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTg                                                                    <  2:5219311/151‑1 (MQ=255)
                                                                                    gCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCt                                                                 <  1:1210721/151‑1 (MQ=255)
                                                                                     ccGCTGGCGATGAGTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCtt                                                                <  1:4003934/151‑1 (MQ=255)
                                                                                       gCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGc                                                              >  2:4451057/1‑151 (MQ=255)
                                                                                        cTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCt                                                             >  1:3267385/1‑151 (MQ=255)
                                                                                         tGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTc                                                            >  1:84719/1‑151 (MQ=255)
                                                                                         tGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTc                                                            >  2:3617110/1‑151 (MQ=255)
                                                                                           gCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGa                                                          <  2:2811814/151‑1 (MQ=255)
                                                                                             gatgatTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGAtt                                                        >  1:5129838/1‑151 (MQ=255)
                                                                                                 cttGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTa                                                    <  2:4845163/150‑1 (MQ=255)
                                                                                                     ccAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAg                                                <  2:4982844/151‑1 (MQ=255)
                                                                                                      cAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGc                                               <  2:1163172/151‑1 (MQ=255)
                                                                                                       aGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCt                                              >  2:1866500/1‑151 (MQ=255)
                                                                                                       aGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCt                                              >  1:2949936/1‑151 (MQ=255)
                                                                                                       aGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCt                                              <  2:4347328/151‑1 (MQ=255)
                                                                                                          cggcgACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGgc                                           >  2:4193637/1‑151 (MQ=255)
                                                                                                              gACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGcgcgc                                       >  1:5410441/1‑150 (MQ=255)
                                                                                                              gACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGa                                       >  1:3634209/1‑151 (MQ=255)
                                                                                                                  ggTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCg                                   <  2:4054635/151‑1 (MQ=255)
                                                                                                                    ttttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTTGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtg                                 >  2:1678989/1‑151 (MQ=255)
                                                                                                                    ttttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtg                                 <  1:788984/151‑1 (MQ=255)
                                                                                                                    ttttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtg                                 >  1:2026371/1‑151 (MQ=255)
                                                                                                                     tttAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtgt                                <  2:3914606/151‑1 (MQ=255)
                                                                                                                     tttAGCGCTAGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCgtgt                                <  2:1139968/151‑1 (MQ=255)
                                                                                                                       tAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGCGTgg                              <  2:4473717/151‑1 (MQ=255)
                                                                                                                           gcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGc                          <  2:4917166/151‑1 (MQ=255)
                                                                                                                           gcTCGGCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGc                          <  2:5077892/151‑1 (MQ=255)
                                                                                                                               ggCCGCTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGc                      >  1:3401066/1‑151 (MQ=255)
                                                                                                                                    cTGTTCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGcc                 >  1:1431841/1‑151 (MQ=255)
                                                                                                                                       ttCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGccctc              >  1:2550290/1‑149 (MQ=255)
                                                                                                                                       ttCAGTTTCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCcgc              >  1:2754273/1‑151 (MQ=255)
                                                                                                                                            tttCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAaca         <  1:2167305/151‑1 (MQ=255)
                                                                                                                                              tCGGCGGGTGGGGGTTTGTCTACGGCAACATTCGTGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACg       <  1:4842876/151‑1 (MQ=255)
                                                                                                                                              tCGGCGGGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACg       >  2:152665/1‑151 (MQ=255)
                                                                                                                                                ggcggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACggg     >  2:2728413/1‑151 (MQ=255)
                                                                                                                                                 gcggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGtgtg                               >  1:3927253/1‑124 (MQ=255)
                                                                                                                                                 gcggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGtgtg                               >  1:210059/1‑124 (MQ=255)
                                                                                                                                                 gcggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGtgtg                               <  2:210059/124‑1 (MQ=255)
                                                                                                                                                  cggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCg   >  1:1020368/1‑151 (MQ=255)
                                                                                                                                                  cggGTGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCg   >  2:958432/1‑151 (MQ=255)
                                                                                                                                                   gggtgAGGGTTTGTCTACGGCAACATGCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGa  <  2:323857/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GTAGGCATCGTCGGTGGTTTCCAGATAGCGGCCGGTGCTGAACCAGTAGCTGGTGAAGGCCAGCAGGGTGATTGCGGCCAGGCTGCCGCTGGCGATGATTGCCAGGCGGCGACGGGTTTTAGCGCTCGGCCGCTGTTCAGTTTCGGCGGGGGAGGGTTTGTCTACGGCAACATTCATGGTTGGTTTCCATTCTCGAATTGGGCCTGCTGGCTGTCGGTTGTGGGAGCCACTGTCTTGCTCGATTTCTAAAAGCTGGCGCGATCCGTGTGGGAGCGGGCTTGCCCGCGAACACGGGCGA  >  CP009974/3971801‑3972098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑