Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,147,416 C→G intergenic (+668/‑353) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,147,4160CG76.7% 306.1 / 88.7 133intergenic (+668/‑353)RPPX_18425/RPPX_18430enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base C (18/13);  new base G (62/40);  total (80/53)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01

Only 100 of 133 total aligned reads displayed.

ACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCC  >  CP009974/4147274‑4147561
                                                                                                                                              |                                                                                                                                                 
                                         cgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatca                                                                                                 >  1:1737284/1‑151 (MQ=11)
                                           ctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag                                                                                               <  2:4811175/151‑1 (MQ=11)
                                              cGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatc                                                                                            <  1:2079655/151‑1 (MQ=11)
                                               ggggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatca                                                                                           <  2:4895730/151‑1 (MQ=11)
                                                gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                          <  2:4385609/151‑1 (MQ=11)
                                                gggTCCCCGATCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                          <  2:4267123/151‑1 (MQ=11)
                                                  gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaaga                                                                                        <  1:4334967/151‑1 (MQ=11)
                                                      ccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaa                                                                                    <  2:3076077/151‑1 (MQ=14)
                                                       ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGGAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCCTGCCCGTCACCTCCCTCCGCAATGCCTACGTGCGGCCTCCTGAAGTCGCGaagctcaagatcacgatcacgatcaag                                                                                   >  2:4085226/1‑151 (MQ=11)
                                                       ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaat                                                                                   >  1:2112274/1‑150 (MQ=11)
                                                       ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaag                                                                                   >  1:375906/1‑151 (MQ=11)
                                                        tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                  <  2:1253250/148‑1 (MQ=14)
                                                         ctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagat                                                                                 >  1:1470590/3‑151 (MQ=11)
                                                          tcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatc                                                                                <  1:5067014/150‑1 (MQ=14)
                                                          tcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatc                                                                                <  1:2501833/150‑1 (MQ=14)
                                                             ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag                                                                             >  2:1887957/1‑150 (MQ=11)
                                                             ccGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaag                                                                             >  1:4694547/1‑150 (MQ=11)
                                                              cGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaaga                                                                            >  2:5121354/1‑149 (MQ=11)
                                                               ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                           <  2:4762798/151‑4 (MQ=11)
                                                               ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                           >  1:13255/1‑148 (MQ=11)
                                                               ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                           >  1:5219766/1‑148 (MQ=11)
                                                               ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                           >  1:5219767/1‑148 (MQ=11)
                                                               ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                           <  1:3408718/151‑4 (MQ=11)
                                                                gCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagatc                                                                          >  1:1750742/1‑147 (MQ=11)
                                                                  cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                         >  1:2919426/1‑151 (MQ=14)
                                                                  cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                         >  1:3891175/1‑151 (MQ=2)
                                                                  cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                         <  1:2229093/151‑1 (MQ=2)
                                                                  cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                         >  1:2919382/1‑151 (MQ=2)
                                                                   ttGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaa                                                                        <  1:1451464/151‑1 (MQ=2)
                                                                     gCTCCCGGCAGGACCGCGCCGCAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGGTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGCTCAAGATcaacggcaacg                                                                      >  2:5173574/1‑151 (MQ=11)
                                                                     gCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacg                                                                      >  2:830812/1‑151 (MQ=17)
                                                                     gCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacg                                                                      >  2:2355827/1‑151 (MQ=17)
                                                                        cccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggca                                                                   >  2:695147/1‑151 (MQ=21)
                                                                         ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaa                                                                  >  2:4458788/1‑151 (MQ=255)
                                                                          cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGCTCAAGATcaacggcaacggcaac                                                                 >  2:2265657/1‑151 (MQ=255)
                                                                          cGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGACATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaac                                                                 <  1:274615/151‑1 (MQ=255)
                                                                           ggCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacg                                                                >  2:668293/1‑151 (MQ=255)
                                                                               ggACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaa                                                            <  2:4831223/151‑1 (MQ=255)
                                                                               ggACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaa                                                            <  2:4061511/151‑1 (MQ=255)
                                                                               ggACCGCGCAGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaa                                                            >  1:3247008/1‑151 (MQ=255)
                                                                                gACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaac                                                           <  2:4243362/151‑1 (MQ=255)
                                                                                gACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaac                                                           >  1:5139298/1‑151 (MQ=255)
                                                                                   cgcgCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCaacggcaacggcaacggcaacggc                                                        <  2:850631/151‑1 (MQ=255)
                                                                                   cgcgCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCaacggcaacggcaacggcaaccgc                                                        >  2:915519/1‑151 (MQ=255)
                                                                                    gcgcCGAAGGCCGCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                       <  2:4641766/151‑1 (MQ=255)
                                                                                    gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                       >  2:1022607/1‑151 (MQ=255)
                                                                                      gcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACggcaacggcaacggcaacggcaac                                                     <  2:2228223/151‑1 (MQ=255)
                                                                                      gcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACggcaacggcaacggcaacggcaac                                                     >  2:1354698/1‑151 (MQ=255)
                                                                                        cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCCACGGCAACGGCAACGgcaacag                                                   >  2:2837179/1‑151 (MQ=255)
                                                                                         gAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagc                                                  >  1:325334/1‑151 (MQ=255)
                                                                                          aaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCACCGGCAACGGCAACGGCAACGgcaacagca                                                 >  2:3425703/1‑151 (MQ=255)
                                                                                          aaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                 >  2:741085/1‑151 (MQ=255)
                                                                                           aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaa                                                >  2:4157520/1‑151 (MQ=255)
                                                                                           aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaa                                                >  2:3292031/1‑151 (MQ=255)
                                                                                            ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaac                                               <  1:3228898/151‑1 (MQ=255)
                                                                                            ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaac                                               >  1:2109036/1‑151 (MQ=255)
                                                                                                ccATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAacagcaacagca                                           <  1:3481588/151‑1 (MQ=255)
                                                                                                  aTCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAgg                                         >  1:3665403/1‑151 (MQ=255)
                                                                                                    ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                       <  2:3988952/151‑1 (MQ=255)
                                                                                                    ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                       >  1:10442/1‑151 (MQ=255)
                                                                                                     cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                      <  1:4580552/151‑1 (MQ=255)
                                                                                                      tGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGaa                                     >  2:5249003/1‑151 (MQ=255)
                                                                                                       ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                    <  2:1897404/151‑1 (MQ=255)
                                                                                                       ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                    <  1:3126753/151‑1 (MQ=255)
                                                                                                        gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                   >  1:1529901/1‑151 (MQ=255)
                                                                                                        gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                   <  1:3528400/151‑1 (MQ=255)
                                                                                                         ggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATgg                                  >  1:3309987/1‑151 (MQ=255)
                                                                                                           ccTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGc                                <  2:3921107/151‑1 (MQ=255)
                                                                                                            cTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCa                               >  2:1785975/1‑151 (MQ=255)
                                                                                                              cAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACt                             >  1:1388243/1‑151 (MQ=255)
                                                                                                                 cgcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGcc                          <  1:918736/151‑1 (MQ=255)
                                                                                                                 cgcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGcc                          >  2:371091/1‑151 (MQ=255)
                                                                                                                 cgcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGcc                          >  1:4264493/1‑151 (MQ=255)
                                                                                                                 cgcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGcc                          >  1:4607997/1‑151 (MQ=255)
                                                                                                                  gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGTCAACGGCAACGGCAACAGCAACAGCACGCGAATGTGCACTGCCa                         >  2:1594955/1‑151 (MQ=255)
                                                                                                                  gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                         >  2:5254164/1‑151 (MQ=255)
                                                                                                                  gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                         >  2:2892065/1‑151 (MQ=255)
                                                                                                                  gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                         >  2:2890975/1‑151 (MQ=255)
                                                                                                                  gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                         >  1:4376382/1‑151 (MQ=255)
                                                                                                                  gattGACGGGCATCCATGGCGGTAACCTGCCTCCGCAAGGACTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                         <  2:2903960/149‑1 (MQ=17)
                                                                                                                    ttGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGa                       >  2:1257705/1‑151 (MQ=255)
                                                                                                                     tGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGaa                      <  1:3080543/151‑1 (MQ=255)
                                                                                                                     tGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGaa                      <  1:72103/151‑1 (MQ=255)
                                                                                                                        cGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATca                   >  1:111579/1‑151 (MQ=255)
                                                                                                                        cGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATca                   <  1:4242906/151‑1 (MQ=255)
                                                                                                                        cGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATca                   <  1:4242997/151‑1 (MQ=255)
                                                                                                                         gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                 >  2:1965901/1‑120 (MQ=255)
                                                                                                                           gCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAg                >  2:1508026/1‑151 (MQ=255)
                                                                                                                           gCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAg                >  1:289079/1‑151 (MQ=255)
                                                                                                                             aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt              >  1:4620157/1‑151 (MQ=255)
                                                                                                                               ccATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTta            >  1:1964176/1‑151 (MQ=255)
                                                                                                                                cATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtac           >  1:3510895/1‑151 (MQ=255)
                                                                                                                                cATGCCCGTCACCTGCCGCCGCAAGGCCTGCGGTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtac           <  1:4949417/151‑1 (MQ=255)
                                                                                                                                 aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGAGCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact          <  1:4416873/151‑1 (MQ=255)
                                                                                                                                  tGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtacta         <  2:3704586/151‑1 (MQ=255)
                                                                                                                                      cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgg     <  2:3401258/151‑1 (MQ=255)
                                                                                                                                      cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgg     <  2:3604394/151‑1 (MQ=255)
                                                                                                                                        tCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGcc   >  1:3434226/1‑151 (MQ=255)
                                                                                                                                        tCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGcc   >  1:3203914/1‑151 (MQ=255)
                                                                                                                                        tCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGACCAAGATCAAGATCAACAGCAACGGCAACGTCAACGGCAACAGCATCAGCAGGCGAATGGGCACTGCCAGAACCACAGACTACTACTGGcc   >  2:4404964/1‑151 (MQ=18)
                                                                                                                                              |                                                                                                                                                 
ACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCC  >  CP009974/4147274‑4147561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑