Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,147,431 A→G intergenic (+683/‑338) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,147,4310AG81.7% 389.5 / 78.7 164intergenic (+683/‑338)RPPX_18425/RPPX_18430enoyl‑CoA hydratase/conjugal transfer protein TraR
Reads supporting (aligned to +/- strand):  ref base A (17/13);  new base G (80/54);  total (97/67)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-01

Only 100 of 164 total aligned reads displayed.

CGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGG  >  CP009974/4147315‑4147577
                                                                                                                    |                                                                                                                                                  
cgctCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatca                                                                                                                 >  1:1737284/1‑151 (MQ=11)
   tCCGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaaga                                                                                                              >  1:2883911/1‑151 (MQ=11)
     cGGGGTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatc                                                                                                            <  1:2079655/151‑1 (MQ=11)
       gggTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                                          <  2:4385609/151‑1 (MQ=11)
       gggTCCCCGATCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaa                                                                                                          <  2:4267123/151‑1 (MQ=11)
         gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaaga                                                                                                        <  1:4312940/151‑1 (MQ=11)
         gTCCCCTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaaga                                                                                                        <  1:4334967/151‑1 (MQ=11)
             ccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaa                                                                                                    <  2:3076077/151‑1 (MQ=14)
             ccTCTCTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagaccaagatcaa                                                                                                    >  1:2948721/1‑151 (MQ=11)
              ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGGAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCCTGCCCGTCACCTCCCTCCGCAATGCCTACGTGCGGCCTCCTGAAGTCGCGaagctcaagatcacgatcacgatcaag                                                                                                   >  2:4085226/1‑151 (MQ=11)
              ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaat                                                                                                   >  1:2112274/1‑150 (MQ=11)
              ctctctCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaag                                                                                                   >  1:375906/1‑151 (MQ=11)
               tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                                  <  2:1253250/148‑1 (MQ=14)
               tctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaaga                                                                                                  >  2:1374548/4‑151 (MQ=14)
                ctcTCCGGCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagat                                                                                                 >  1:1470590/3‑151 (MQ=11)
                      ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                                           >  1:13255/1‑148 (MQ=11)
                      ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                                           <  2:4762798/151‑4 (MQ=11)
                      ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                                           >  1:5219767/1‑148 (MQ=11)
                      ggCCTCGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAgatcaagatcaagatcaagatcaagatcaagat                                                                                           <  1:3408718/151‑4 (MQ=11)
                         cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                                         <  1:2229093/151‑1 (MQ=2)
                         cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                                         >  1:3891175/1‑151 (MQ=2)
                         cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                                         >  2:789272/1‑151 (MQ=14)
                         cTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggca                                                                                         >  1:2919382/1‑151 (MQ=2)
                            gCTCCCGGCAGGACCGCGCCGCAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGGTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGCTCAAGATcaacggcaacg                                                                                      >  2:5173574/1‑151 (MQ=11)
                            gCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacg                                                                                      >  2:830812/1‑151 (MQ=17)
                            gCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacg                                                                                      >  2:2355827/1‑151 (MQ=17)
                                ccGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaa                                                                                  >  2:4458788/1‑151 (MQ=255)
                                 gggCAGGACCGCGCGGAAGGCCCCATCCTGGGGCCGCAGCGGTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGAGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaac                                                                                 <  1:2140334/150‑1 (MQ=11)
                                  ggCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacg                                                                                >  2:668293/1‑151 (MQ=255)
                                      ggACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaa                                                                            <  2:4061511/151‑1 (MQ=255)
                                      ggACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaa                                                                            <  2:4831223/151‑1 (MQ=255)
                                       gACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaac                                                                           >  1:5139298/1‑151 (MQ=255)
                                       gACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATcaacggcaacggcaacggcaac                                                                           <  1:4622381/151‑1 (MQ=255)
                                          cgcgCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCaacggcaacggcaacggcaaccgc                                                                        >  2:915519/1‑151 (MQ=255)
                                           gcgcCGAAGGCCGCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                                       <  2:4641766/151‑1 (MQ=255)
                                           gcgcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAacggcaacggcaacggcaacggca                                                                       >  2:1022607/1‑151 (MQ=255)
                                             gcCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACggcaacggcaacggcaacggcaac                                                                     <  2:2228223/151‑1 (MQ=255)
                                              ccGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaaca                                                                    >  2:1836563/1‑151 (MQ=255)
                                               cGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCCACGGCAACGGCAACGgcaacag                                                                   >  2:2837179/1‑151 (MQ=255)
                                                gAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagc                                                                  >  1:325334/1‑151 (MQ=255)
                                                 aaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                                 >  2:741085/1‑151 (MQ=255)
                                                  aGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaa                                                                >  2:4157520/1‑151 (MQ=255)
                                                   ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaac                                                               >  2:2899353/1‑151 (MQ=255)
                                                   ggCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaac                                                               <  1:3228898/151‑1 (MQ=255)
                                                    gCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagcaacc                                                              >  2:2688864/1‑150 (MQ=255)
                                                       ccATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCACCGGCAACGGCAccagcaacagca                                                           >  1:508249/1‑151 (MQ=255)
                                                       ccATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAacagcaacagca                                                           <  1:3481588/151‑1 (MQ=255)
                                                         aTCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAgg                                                         >  1:3665403/1‑151 (MQ=255)
                                                           ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                                       >  1:10442/1‑151 (MQ=255)
                                                           ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                                       <  2:3988905/151‑1 (MQ=255)
                                                           ccTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCg                                                       <  2:3988952/151‑1 (MQ=255)
                                                            cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                                      <  1:4580552/151‑1 (MQ=255)
                                                            cTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGa                                                      >  1:343892/1‑151 (MQ=255)
                                                              ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                                    <  2:1897404/151‑1 (MQ=255)
                                                              ggggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAAt                                                    <  1:3126753/151‑1 (MQ=255)
                                                               gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                                   <  1:3528400/151‑1 (MQ=255)
                                                               gggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATg                                                   >  1:1529901/1‑151 (MQ=255)
                                                                ggCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATgg                                                  >  1:3309987/1‑151 (MQ=255)
                                                                 gCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGACGTCGCGAAGATCAAGATCAAGATCAAGCTCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGACTggg                                                 >  2:4822012/1‑151 (MQ=14)
                                                                     cAGCGCTTGGCGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACt                                             >  1:1388262/1‑151 (MQ=255)
                                                                         gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGTCAACGGCAACGGCAACAGCAACAGCACGCGAATGTGCACTGCCa                                         >  2:1594955/1‑151 (MQ=255)
                                                                         gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                                         >  2:5254164/1‑151 (MQ=255)
                                                                         gcTTGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCa                                         >  2:2892065/1‑151 (MQ=255)
                                                                           ttGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGa                                       >  2:1257705/1‑151 (MQ=255)
                                                                            tGACGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGaa                                      <  1:72103/151‑1 (MQ=255)
                                                                               cGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATca                                   >  1:111579/1‑151 (MQ=255)
                                                                               cGGGCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATca                                   <  1:4242997/151‑1 (MQ=255)
                                                                                gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                                 >  2:1965901/1‑120 (MQ=255)
                                                                                gggCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGgcaacagca                                                                 <  1:1965901/120‑1 (MQ=255)
                                                                                  gCATCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAg                                <  2:3308758/151‑1 (MQ=255)
                                                                                    aTCCATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGtt                              >  1:4620157/1‑151 (MQ=255)
                                                                                      ccATGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTta                            >  1:1964176/1‑151 (MQ=255)
                                                                                       cATGCCCGTCACCTGCCTCCGCCAGGCCGGCGTGCGGCCTGGTGAAGTGGCGAAGATCAAGATCAAGATCAAGATCAAGAGCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtac                           <  2:4297568/151‑1 (MQ=18)
                                                                                       cATGCCCGTCACCTGCCGCCGCAAGGCCTGCGGTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtac                           <  1:4949417/151‑1 (MQ=255)
                                                                                        aTGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGAGCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtact                          <  1:4416873/151‑1 (MQ=255)
                                                                                         tGCCCGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtacta                         <  2:3704586/151‑1 (MQ=255)
                                                                                          gCCCGTCACCGGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGAGCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTtactac                        <  2:5166527/151‑1 (MQ=255)
                                                                                            ccGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTg                      >  1:3992421/1‑151 (MQ=255)
                                                                                             cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgg                     <  2:3604491/151‑1 (MQ=255)
                                                                                             cGTCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTgg                     <  2:3401258/151‑1 (MQ=255)
                                                                                               tCACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGACCAAGATCAAGATCAACAGCAACGGCAACGTCAACGGCAACAGCATCAGCAGGCGAATGGGCACTGCCAGAACCACAGACTACTACTGGcc                   >  2:4404964/1‑151 (MQ=18)
                                                                                                cACCTGCCTCCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTCGccc                  >  1:3227549/1‑151 (MQ=255)
                                                                                                   ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGCTCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGGATGGGCACTGCCAGAATCACAGTCTACTACCGGCGCTTc               >  2:3580496/4‑151 (MQ=18)
                                                                                                   ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTc               <  1:2649676/148‑1 (MQ=255)
                                                                                                   ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTc               >  2:4998856/4‑151 (MQ=255)
                                                                                                   ctgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTc               <  2:2303308/148‑1 (MQ=255)
                                                                                                    tgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCa              <  1:5411761/149‑1 (MQ=255)
                                                                                                    tgcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCa              >  2:2612196/3‑151 (MQ=255)
                                                                                                     gcctccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCag             <  1:4473614/150‑1 (MQ=255)
                                                                                                      cctccGCAGGGCGGGCGGTCGGCCGCCTGAAGTCGCGAAGATCAAGAGCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagc            <  1:3002918/151‑1 (MQ=25)
                                                                                                        tccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctg          <  1:956570/151‑1 (MQ=255)
                                                                                                        tccGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctg          <  1:957801/151‑1 (MQ=255)
                                                                                                          cGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgag        <  2:4272567/151‑1 (MQ=255)
                                                                                                           gCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagc       >  1:414575/1‑151 (MQ=255)
                                                                                                            cAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGGTCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagct      <  2:3734906/151‑1 (MQ=255)
                                                                                                             aaGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagctg     >  1:2879416/1‑151 (MQ=255)
                                                                                                             aaGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCagctgagctg     >  2:1638412/1‑151 (MQ=255)
                                                                                                              aGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCCGGCGAATGGGCACTGCCAGAATCACCGTTTACTACTGGCCCTTCAGCTGAGCTgg    <  2:5345/151‑1 (MQ=255)
                                                                                                              aGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTgg    >  1:2186807/1‑151 (MQ=255)
                                                                                                                gCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTgggg  >  1:1308925/1‑151 (MQ=255)
                                                                                                                    |                                                                                                                                                  
CGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAAGGCCTACGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAACGGCAACGGCAACGGCAACGGCAACAGCAACAGCAGGCGAATGGGCACTGCCAGAATCACAGTTTACTACTGGCCCTTCAGCTGAGCTGGGG  >  CP009974/4147315‑4147577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑