Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,537,108	A→G	Y105Y (TAT→TAC)	RPPX_20270 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,537,108	0	A	G	100.0%	240.2 / NA	76	Y105Y (TAT→TAC)	RPPX_20270	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/38);  total (38/38)

GTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACC  >  CP009974/4536960‑4537166                                                                                                                                                     |                                                           gtggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTa                                                          <  1:2876947/151‑1 (MQ=255) gtggcgCTGCTCGGACGTTGAACAGGCAGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTa                                                          >  2:3575391/1‑151 (MQ=255)  tggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAg                                                         >  1:2879290/1‑151 (MQ=255)   ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                        >  2:740448/1‑151 (MQ=255)   ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                        >  2:4897773/1‑151 (MQ=255)   ggcgCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGt                                                        <  1:3708871/151‑1 (MQ=255)    gcgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTg                                                       <  1:3287269/151‑1 (MQ=255)    gcgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTg                                                       <  1:2309106/151‑1 (MQ=255)     cgcTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTgg                                                      >  2:1739895/1‑151 (MQ=255)      gctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgc                                                     >  1:1151823/1‑151 (MQ=255)       ctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcg                                                    >  1:5237390/1‑151 (MQ=255)       ctgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcg                                                    <  1:1573133/151‑1 (MQ=255)        tgctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGgcgc                                                   >  1:2457673/1‑151 (MQ=255)         gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                                  <  2:1443735/151‑1 (MQ=255)         gctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGcc                                                  <  2:1444921/151‑1 (MQ=255)          ctCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCa                                                 <  2:2745987/151‑1 (MQ=255)           tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                                >  1:3141584/1‑151 (MQ=255)           tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                                <  1:1298000/151‑1 (MQ=255)           tCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAg                                                >  1:3105943/1‑151 (MQ=255)            cGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAgg                                               >  2:133986/1‑151 (MQ=255)            cGGACGTTGAACAGGCGGCAGAGGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCGCCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAgg                                               <  2:2665340/151‑1 (MQ=255)              gACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                             <  2:145369/151‑1 (MQ=255)              gACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCt                                             >  1:1388750/1‑151 (MQ=255)               aCGTTGAACAGGCGGCAGATGCCATACCAGCAGGGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGACCGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                            <  1:3221763/151‑1 (MQ=255)               aCGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCtt                                            <  1:3221850/151‑1 (MQ=255)                cGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTc                                           <  2:4826592/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                          <  2:4846666/151‑1 (MQ=255)                 gTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCt                                          >  1:3241325/1‑151 (MQ=255)                  ttGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTg                                         <  2:971897/151‑1 (MQ=255)                  ttGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCACGCTTCTg                                         >  1:4817373/1‑151 (MQ=255)                   tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                        >  1:2165827/1‑151 (MQ=255)                   tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                        <  1:490112/151‑1 (MQ=255)                   tGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGc                                        <  2:18129/151‑1 (MQ=255)                    gAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGcc                                       <  1:3315893/151‑1 (MQ=255)                    gAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGcc                                       >  1:1004745/1‑151 (MQ=255)                     aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTCGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                                      <  2:5252040/151‑1 (MQ=255)                     aaCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCg                                      <  1:4938015/151‑1 (MQ=255)                      aCAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgg                                     >  1:1159422/1‑151 (MQ=255)                       cAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCggg                                    <  2:297056/151‑1 (MQ=255)                        aGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgggg                                   <  1:3356152/151‑1 (MQ=255)                        aGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgggg                                   >  1:2626109/1‑151 (MQ=255)                        aGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCgggg                                   >  2:2392291/1‑151 (MQ=255)                         ggcggcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGt                                  <  1:4621095/151‑1 (MQ=255)                             gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                              >  1:1994011/1‑151 (MQ=255)                             gcAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAgg                              >  1:578167/1‑151 (MQ=255)                               aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCGGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTCCTGCCGGGGGGAGGCa                            >  2:2756843/1‑151 (MQ=255)                               aGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCa                            >  1:2628271/1‑151 (MQ=255)                                gATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAg                           >  1:1886982/1‑151 (MQ=255)                                 aTGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGa                          >  2:1534799/1‑151 (MQ=255)                                  tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                         >  2:4736345/1‑151 (MQ=255)                                  tGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGAt                         >  2:290011/1‑151 (MQ=255)                                   gCCATACCCGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGACCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                        <  2:5237917/151‑1 (MQ=255)                                   gCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATg                        <  2:3286991/151‑1 (MQ=255)                                    ccATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATgg                       <  1:1402505/151‑1 (MQ=255)                                    ccATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGGGAGGCAGATgg                       >  2:3259880/1‑151 (MQ=255)                                      aTACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCg                     >  1:1460668/1‑151 (MQ=25)                                      aTACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCg                     <  2:408391/151‑1 (MQ=255)                                       tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt                    <  1:868632/151‑1 (MQ=19)                                       tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt                    <  2:363728/151‑1 (MQ=255)                                       tACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGt                    <  1:3121326/151‑1 (MQ=16)                                        aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg                   <  2:3736077/151‑1 (MQ=255)                                        aCCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTg                   >  1:3883433/1‑151 (MQ=21)                                         gcaggagAGGGCGACGGTCAGGTTGGAGGGGCACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCAGTTCACCGGGCGCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGc                  <  2:4458608/150‑1 (MQ=11)                                          cagcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCa                 <  2:503933/151‑1 (MQ=18)                                           agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAg                >  1:840767/1‑151 (MQ=18)                                           agcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAg                >  1:1690204/1‑151 (MQ=18)                                            gcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGt               >  2:285286/1‑151 (MQ=18)                                            gcagAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGt               >  1:1190226/1‑151 (MQ=16)                                              agagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTgg             >  2:3504514/1‑151 (MQ=17)                                               gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc            <  2:1139526/151‑1 (MQ=9)                                               gagGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGc            <  1:2883896/151‑1 (MQ=12)                                                agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt           >  1:1913058/1‑151 (MQ=9)                                                agGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCt           >  1:2351626/1‑151 (MQ=9)                                                 gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCCGATGGCGTGCAGTGGCtt          >  2:4838059/1‑151 (MQ=9)                                                 gggCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCtt          <  1:432094/151‑1 (MQ=11)                                                         gtaaGGTTGGAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCGTAGTGGCGCCAGGCTTCTGCCGGGGTGAGGCAGATGGCGTGCAGTGGCTTGTGccacc  <  2:1538798/148‑1 (MQ=2)                                                                                                                                                     |                                                           GTGGCGCTGCTCGGACGTTGAACAGGCGGCAGATGCCATACCAGCAGAGGGCGACGGTCAGGTTGTAGGGGAACAGCACGGCCCAGAACGGCAATTGCCAGTTCTTTTTGCCCTGCATTTCACCGGGCTCGCCGGTTCGCCAGGGGGCATAGTGGCGCCAGGCTTCTGCCGGGGTCAGGCAGATGGCGTGCAGTGGCTTGTGCCACC  >  CP009974/4536960‑4537166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑