BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,540,457	A→G	R459R (CGT→CGC)	RPPX_20280 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,540,457	0	A	G	99.5%	660.0 / NA	208	R459R (CGT→CGC)	RPPX_20280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (101/106); minor base C (0/1); total (101/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

AGCGCCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAACGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGATG  >  CP009974/4540309‑4540604

aGCGCCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCg                                                                                                                                                   <  1:5132722/151‑1 (MQ=255)

 gcgcCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCgt                                                                                                                                                  <  2:2869095/151‑2 (MQ=255)

  cgcCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGACGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCgtc                                                                                                                                                 <  1:4421795/151‑3 (MQ=255)

    ccccGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCgg                                                                                                                                               >  1:1512660/1‑151 (MQ=255)

    ccccGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCgg                                                                                                                                               <  2:1943810/151‑1 (MQ=255)

         ccAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATg                                                                                                                                          <  1:4642537/151‑1 (MQ=255)

           aaTCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGAt                                                                                                                                        <  2:976565/151‑1 (MQ=255)

            accccTGGCTCACGTGGTCGCTGGCGCGGAACAGGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGGCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATc                                                                                                                                       <  2:3203163/149‑1 (MQ=14)

            aTCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGAtt                                                                                                                                       >  2:1582232/1‑150 (MQ=255)

             tCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCt                                                                                                                                      <  1:4683221/151‑1 (MQ=255)

             tCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCGGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTGTGAAGCGTCGGGCATGATCt                                                                                                                                      >  2:252321/1‑151 (MQ=255)

               ccTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTc                                                                                                                                    >  1:3556358/1‑151 (MQ=255)

                  cGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGt                                                                                                                                 >  1:3112865/1‑151 (MQ=255)

                   gATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTg                                                                                                                                <  1:3806216/151‑1 (MQ=255)

                      cACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCg                                                                                                                             <  1:4643083/151‑1 (MQ=255)

                      cACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCg                                                                                                                             <  1:1349629/151‑1 (MQ=255)

                           tGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGgtgt                                                                                                                        >  1:5340124/1‑151 (MQ=255)

                           tGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGgtgt                                                                                                                        <  1:3368669/151‑1 (MQ=255)

                             tCGTTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGACCGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCg                                                                                                                      <  2:4100045/151‑1 (MQ=255)

                              cGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCgg                                                                                                                     >  1:2479389/1‑151 (MQ=255)

                               gCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGCGTCGGc                                                                                                                    >  2:1439949/1‑151 (MQ=255)

                                 tGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTg                                                                                                                  <  2:4782594/151‑1 (MQ=255)

                                   gcgcCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGc                                                                                                                >  2:3096488/1‑151 (MQ=255)

                                     gcCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGccc                                                                                                              >  1:3619319/1‑151 (MQ=255)

                                         aaCAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTg                                                                                                          <  2:222305/151‑1 (MQ=255)

                                         aaCAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCACTg                                                                                                          >  2:2673496/1‑151 (MQ=255)

                                             aGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTg                                                                                                      <  2:1277347/151‑1 (MQ=255)

                                                  ccGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCGAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCTAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCa                                                                                                 <  2:4656031/151‑1 (MQ=255)

                                                     aTGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCa                                                                                              >  2:2464382/1‑151 (MQ=255)

                                                     aTGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCa                                                                                              <  2:273959/151‑1 (MQ=255)

                                                     aTGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCa                                                                                              >  1:2983058/1‑151 (MQ=255)

                                                      tGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCaa                                                                                             >  2:4813858/1‑151 (MQ=255)

                                                       ggCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAAc                                                                                            <  2:1560092/151‑1 (MQ=25)

                                                       ggCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAAc                                                                                            <  1:992373/151‑1 (MQ=255)

                                                        gCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACg                                                                                           >  2:1166984/1‑151 (MQ=18)

                                                         cGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGc                                                                                          <  1:1722239/151‑1 (MQ=16)

                                                          gTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGcc                                                                                         >  2:3668951/1‑151 (MQ=12)

                                                           tCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAATCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGAGCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        <  2:3374509/151‑1 (MQ=255)

                                                           tCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        <  2:2103328/151‑1 (MQ=12)

                                                              gCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTg                                                                                     <  1:1885645/151‑1 (MQ=12)

                                                                        tGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAg                                                                           <  1:94410/151‑1 (MQ=12)

                                                                          aaCGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGAc                                                                         >  2:5214348/1‑151 (MQ=14)

                                                                          aaCGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGAc                                                                         >  2:5310159/1‑151 (MQ=9)

                                                                           aCGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGAcc                                                                        >  1:63762/1‑151 (MQ=9)

                                                                            cGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcg                                                                       >  1:4056660/1‑151 (MQ=16)

                                                                              ggTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACcgcg                                                                     <  2:1740388/151‑1 (MQ=21)

                                                                                 tGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggc                                                                  <  1:1796850/151‑1 (MQ=255)

                                                                                 tGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggc                                                                  <  1:4177907/151‑1 (MQ=255)

                                                                                  gAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCgcgggcg                                                                 >  2:4926873/1‑151 (MQ=21)

                                                                                     cGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAg                                                              <  1:905475/151‑1 (MQ=255)

                                                                                      ggCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAgg                                                             >  2:3065964/1‑151 (MQ=18)

                                                                                        cgcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTg                                                           <  1:3258458/151‑1 (MQ=21)

                                                                                         gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        >  2:1077726/1‑121 (MQ=17)

                                                                                         gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCa                                                                                        <  1:1077726/121‑1 (MQ=16)

                                                                                         gcCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGc                                                          >  2:788749/1‑151 (MQ=19)

                                                                                           cAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGt                                                        >  2:2060907/1‑151 (MQ=19)

                                                                                            aaGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGtt                                                       >  2:2863366/1‑151 (MQ=21)

                                                                                            aaGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGtt                                                       <  1:4480465/151‑1 (MQ=255)

                                                                                            aaGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGtt                                                       >  1:3528322/1‑151 (MQ=19)

                                                                                                 ggTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCTTTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCa                                                  <  2:2323170/151‑1 (MQ=255)

                                                                                                  gtgtAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCAc                                                 <  2:2983058/151‑1 (MQ=255)

                                                                                                  gtgtAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCCTCGGTGCCGGGTGTCCGCTGGCCCATTGGGTGAATCACCAACGCCAGTCAGATTTCCAGACCGCGGGCGGAGGCGCGCTCgccgc                                                 >  2:2269406/1‑147 (MQ=11)

                                                                                                      aaaGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTc                                             <  2:3740726/151‑1 (MQ=255)

                                                                                                      aaaGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTc                                             <  1:2777198/151‑1 (MQ=255)

                                                                                                      aaaGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTc                                             <  2:4524801/151‑1 (MQ=255)

                                                                                                       aaGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCg                                            <  2:3948452/151‑1 (MQ=255)

                                                                                                             gCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAg                                      >  1:2229387/1‑151 (MQ=25)

                                                                                                                gTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGcc                                   <  1:5214348/151‑1 (MQ=255)

                                                                                                                   gTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAGCGCCAGTGAGATTTCTAGACCGCGGGCGGCGGTGCGTTCGTCACGGTCGTAACAGGCCAAg                                >  1:5261621/1‑151 (MQ=255)

                                                                                                                    tGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCGTTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGGTCGTCACGGTCGTAACAGGCGAAgg                               >  1:3901937/1‑151 (MQ=255)

                                                                                                                    tGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAgg                               <  1:1889432/151‑1 (MQ=255)

                                                                                                                    ggCTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAgg                               <  1:4844000/150‑1 (MQ=255)

                                                                                                                     gCTGGTCCAACCATTGCTCCAGGCGTTTGAACCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCAATGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggc                              <  2:4255291/151‑1 (MQ=255)

                                                                                                                      cTGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggcg                             >  1:2167223/1‑151 (MQ=255)

                                                                                                                       tGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggcgg                            <  2:5409708/151‑1 (MQ=255)

                                                                                                                       tGGTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAggcgg                            <  1:264877/151‑1 (MQ=255)

                                                                                                                         gTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCACGGCGGCt                          >  2:4390099/1‑151 (MQ=255)

                                                                                                                         gTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCt                          <  1:545442/151‑1 (MQ=255)

                                                                                                                         gTCCAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCt                          <  2:5170665/151‑1 (MQ=255)

                                                                                                                           ccAGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGc                        >  1:1145386/1‑151 (MQ=255)

                                                                                                                             aGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCa                      <  2:4223068/151‑1 (MQ=25)

                                                                                                                             aGCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCa                      <  2:2112689/151‑1 (MQ=255)

                                                                                                                              gCCATTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAg                     >  2:5255765/1‑151 (MQ=255)

                                                                                                                                 aTTGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCCGGCt                  >  1:5048473/1‑151 (MQ=255)

                                                                                                                                   tGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGc                        <  2:93171/143‑1 (MQ=255)

                                                                                                                                   tGCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGc                        >  1:93171/1‑143 (MQ=255)

                                                                                                                                    gCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTa               <  1:1704084/151‑1 (MQ=255)

                                                                                                                                    gCTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTa               <  1:3991961/151‑1 (MQ=255)

                                                                                                                                     cTCCAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAg              >  1:4719288/1‑151 (MQ=25)

                                                                                                                                       ccAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAggg            >  2:836943/1‑151 (MQ=255)

                                                                                                                                        cAGGCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAgggg           >  2:3024013/1‑151 (MQ=255)

                                                                                                                                        cAGGCGTTTGAAGCGTCGGTCATGATCGTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAgggg           <  1:2836806/151‑1 (MQ=255)

                                                                                                                                          ggCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGtt         >  2:3907252/1‑151 (MQ=255)

                                                                                                                                          ggCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGtt         >  2:3612105/1‑151 (MQ=255)

                                                                                                                                           gCGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTa        >  2:1732971/1‑151 (MQ=255)

                                                                                                                                            cGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTAt       <  1:3218256/151‑1 (MQ=255)

                                                                                                                                            cGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTAt       <  1:207060/151‑1 (MQ=255)

                                                                                                                                            cGTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGCGTCGGCTGGCCCATTGGGTGAATAAGCAACGCCAGTGAGATTTCCAGACCGGGGGCGGAGGTGCGTTCGTCACGCTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTAt       >  2:4148500/1‑151 (MQ=11)

                                                                                                                                             gTTTGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATc      >  1:1560991/1‑151 (MQ=255)

                                                                                                                                              tttGAAGCGTCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCg     >  1:2581233/1‑151 (MQ=255)

AGCGCCCCGCCAATCCCTCGATCACGTTGTCGCTGGCGCCGAACAAGGAGCCGATGGCGTCGGCTTTGTCCTTGAACGGGTTGAACGGCGCCAAGGCGGTGTAAAGCGGGCTGTCGTGCTGGTCCAGCCATTGCTCCAGGCGTTTGAAACGCCGGTCATGATCTTCGGTGCCGGGTGTCGGCTGGCCCATTGGGTGAATCAGCAACGCCAGTGAGATTTCCAGACCGCGGGCGGAGGTGCGTTCGTCACGGTCGTAACAGGCCAAGGCGGCTGCCAGGCTGTAGGGGTTATCGATG  >  CP009974/4540309‑4540604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`