BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,541,636	C→T	P66P (CCG→CCA)	RPPX_20280 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,541,636	0	C	T	98.9%	477.4 / NA	176	P66P (CCG→CCA)	RPPX_20280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (89/85); minor base G (0/2); total (89/87)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

CGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCACGCGT  >  CP009974/4541488‑4541783

cGGTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTgg                                                                                                                                                   <  1:2774804/151‑1 (MQ=25)

 ggTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTggg                                                                                                                                                  <  1:3733519/151‑1 (MQ=25)

 ggTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTggg                                                                                                                                                  <  1:3694627/151‑1 (MQ=25)

 ggTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTggg                                                                                                                                                  <  2:1514485/151‑1 (MQ=25)

 ggTGCTCTGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTggg                                                                                                                                                  <  1:1887810/151‑1 (MQ=25)

      tctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaa                                                                                                                                             <  1:3924466/151‑1 (MQ=19)

      tctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaa                                                                                                                                             <  1:995977/151‑1 (MQ=19)

      tctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaa                                                                                                                                             >  2:1132236/1‑151 (MQ=255)

       ctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaaa                                                                                                                                            >  2:2178798/1‑151 (MQ=19)

       ctGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCaaa                                                                                                                                            <  1:2986757/151‑1 (MQ=25)

        tGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAg                                                                                                                                           <  1:3161052/151‑1 (MQ=25)

        tGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAg                                                                                                                                           <  1:2239836/151‑1 (MQ=255)

        tGGCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAg                                                                                                                                           <  1:5219326/151‑1 (MQ=18)

         ggCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGc                                                                                                                                          <  2:4661592/151‑1 (MQ=25)

          gCCGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcg                                                                                                                                         >  1:732311/1‑150 (MQ=255)

           ccGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgc                                                                                                                                        >  2:1401336/1‑149 (MQ=255)

           ccGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgc                                                                                                                                        >  2:4444418/1‑149 (MQ=255)

            cGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgcg                                                                                                                                       <  2:5244509/151‑1 (MQ=255)

            cGGATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGcgcg                                                                                                                                       >  1:3857517/1‑151 (MQ=255)

             ggATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCgg                                                                                                                                      >  2:1781472/1‑151 (MQ=255)

             ggATAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCgg                                                                                                                                      >  2:1415568/1‑151 (MQ=255)

              gataGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGa                                                                                                                                     >  2:2031667/4‑151 (MQ=255)

                taGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAgg                                                                                                                                   <  1:4457299/150‑1 (MQ=255)

                taGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAgg                                                                                                                                   >  1:608074/2‑151 (MQ=255)

                 aGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAggg                                                                                                                                  >  2:5047220/1‑151 (MQ=25)

                 aGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAggg                                                                                                                                  >  1:4928697/1‑151 (MQ=25)

                  ggTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGgt                                                                                                                                 <  2:4122514/151‑1 (MQ=255)

                      gcgcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAg                                                                                                                             <  2:5009609/151‑1 (MQ=255)

                      gcgcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAg                                                                                                                             <  2:1550804/151‑1 (MQ=255)

                       cgcTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAgg                                                                                                                            >  2:462509/1‑151 (MQ=255)

                            aCGGCGGTGGTAGTCCTCCAGCCCTTGGGAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAg                                                                                                                       <  2:3950150/151‑1 (MQ=255)

                              ggcggTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGtg                                                                                                                     <  2:2636621/151‑1 (MQ=19)

                                 ggtggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGtgctg                                                                                                                  >  1:286590/1‑151 (MQ=255)

                                  gtggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGtgctgc                                                                                                                 <  1:4142205/151‑1 (MQ=255)

                                   tggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCa                                                                                                                >  2:2918153/1‑151 (MQ=255)

                                   tggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCa                                                                                                                <  1:250615/151‑1 (MQ=19)

                                   tggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCa                                                                                                                <  1:252327/151‑1 (MQ=255)

                                    ggtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGCGTAGGCCGAGGGCTGCac                                                                                                               >  2:3342658/1‑150 (MQ=255)

                                     gtAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGc                                                                                                              <  1:2993648/151‑1 (MQ=16)

                                       aGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGCCGCAAAGCGCGGAGGGTGTAGGCCGCGTGCTGCcgcgg                                                                                                            >  2:2615049/1‑150 (MQ=11)

                                        gTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTg                                                                                                           <  2:3162911/151‑1 (MQ=19)

                                         tcctccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTgg                                                                                                          <  1:76126/151‑1 (MQ=255)

                                         tcctccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTgg                                                                                                          <  2:950360/151‑1 (MQ=255)

                                         tcctccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTgg                                                                                                          <  1:122933/151‑1 (MQ=18)

                                         tcctccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGGGCTGCAGCGTgg                                                                                                          >  2:1060201/1‑151 (MQ=255)

                                         tcctccAGCCCTTGGTAGCACAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGGGTAGGCCGAGTGCTGCAGCGTgg                                                                                                          >  2:25978/1‑151 (MQ=9)

                                          cctccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTggg                                                                                                         >  1:2304820/1‑151 (MQ=25)

                                             ccAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGaa                                                                                                      <  2:2769137/151‑1 (MQ=19)

                                              cAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAg                                                                                                     >  2:298555/1‑151 (MQ=21)

                                               aGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGGAGc                                                                                                    >  2:3260022/1‑151 (MQ=255)

                                               aGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGc                                                                                                    >  1:5327985/1‑151 (MQ=255)

                                                   cTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTg                                                                                                <  2:374290/151‑1 (MQ=16)

                                                    ttGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGt                                                                                               >  1:663352/1‑151 (MQ=19)

                                                    ttGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGt                                                                                               <  2:1876542/151‑1 (MQ=19)

                                                     tGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGCGCTGCAGCGTGGGGAAGCCTTGtt                                                                                              >  1:4246111/1‑151 (MQ=255)

                                                       gTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTcc                                                                                            >  2:2362233/1‑151 (MQ=18)

                                                        tAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCa                                                                                           <  1:1620705/151‑1 (MQ=14)

                                                        tAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCa                                                                                           <  2:2267875/151‑1 (MQ=9)

                                                         agctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAg                                                                                          >  2:3436886/1‑151 (MQ=9)

                                                          gctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAgg                                                                                         >  2:4601053/1‑151 (MQ=9)

                                                          gctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAgg                                                                                         >  1:330552/1‑151 (MQ=14)

                                                          gctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAgg                                                                                         <  2:117933/151‑1 (MQ=9)

                                                           ctcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGt                                                                                        <  2:883864/151‑1 (MQ=14)

                                                            tcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGtt                                                                                       >  1:497576/1‑151 (MQ=11)

                                                            tcagctcTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGtt                                                                                       <  2:2224009/151‑1 (MQ=11)

                                                                                                      ccAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGt                                             >  2:1618195/1‑151 (MQ=2)

                                                                                                      ccAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGt                                             <  1:2227715/151‑1 (MQ=2)

                                                                                                       cAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTg                                            >  2:1236792/1‑151 (MQ=9)

                                                                                                       cAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTg                                            >  1:811510/1‑151 (MQ=9)

                                                                                                        agctggtCGCCCTGAGGGCCTTTCATGAACACGTAGACATGGCCGGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGc                                           <  2:4632281/145‑1 (MQ=11)

                                                                                                        aGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGc                                           <  1:4913484/151‑1 (MQ=14)

                                                                                                        aGCTTTTCGCCCTGAGGGCCTGTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGc                                           <  1:1810215/151‑1 (MQ=9)

                                                                                                           ttttCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCGTAGCGGCAGGCGGGGGCGTCGCCGGTGCggg                                        >  2:2187474/1‑151 (MQ=9)

                                                                                                           ttttCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATCGCGGCAGGCGGGGCCGTCGCCGGTGCggg                                        >  2:4396439/1‑151 (MQ=9)

                                                                                                               cGCCCTGAGGGCCTTTCATGGACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCAc                                    <  1:1780995/151‑1 (MQ=9)

                                                                                                                 cccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGa                                  >  1:455042/1‑151 (MQ=12)

                                                                                                                 cccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGa                                  >  1:5422963/1‑151 (MQ=12)

                                                                                                                 cccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGa                                  >  1:5339665/1‑151 (MQ=12)

                                                                                                                 cccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGa                                  >  1:3786526/1‑151 (MQ=12)

                                                                                                                  ccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGTGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGAt                                 >  1:1522751/1‑151 (MQ=9)

                                                                                                                  ccTGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGAt                                 <  1:1278949/151‑1 (MQ=17)

                                                                                                                    tGAGGGCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATgg                               >  2:2322071/1‑151 (MQ=9)

                                                                                                                     gAGGGCCTTTCATGAACACGTAGACATAGCCTTTGCGTAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACTATggt                              >  2:1232430/1‑150 (MQ=11)

                                                                                                                        ggCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTa                           <  1:450868/151‑1 (MQ=12)

                                                                                                                        ggCCTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTa                           >  1:169934/1‑151 (MQ=9)

                                                                                                                          ccTTTCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAgc                         <  1:2442603/151‑1 (MQ=17)

                                                                                                                            tttCATGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAgcgc                       <  1:1470615/151‑1 (MQ=12)

                                                                                                                               cATGAACTCGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGCGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCAcc                    <  1:1220457/151‑1 (MQ=255)

                                                                                                                                 tGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCgg                  >  2:2532234/1‑151 (MQ=16)

                                                                                                                                 tGAACACGTAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCgg                  >  1:700933/1‑151 (MQ=19)

                                                                                                                                        gTAGACATAGCCTGGGCGCAAAGCGCGGTGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTACCGCACCGGCAGGATa           >  1:1988424/1‑151 (MQ=255)

                                                                                                                                         tAGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGCGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAg          >  2:3248311/1‑151 (MQ=255)

                                                                                                                                          aGACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAgg         >  1:1517407/1‑151 (MQ=255)

                                                                                                                                           gACATAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGc        <  2:4308420/151‑1 (MQ=255)

                                                                                                                                             cATAGCTTGGGCGCAAGGCGCGGAGGGTGTAGGCCGAGTGCTGAAGCGGGGGAAAGCCTTGTTGCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCATCGGCAGGATAGGCAc      <  2:608534/151‑1 (MQ=11)

                                                                                                                                              aTAGGCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGGGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcg     <  2:4450859/151‑1 (MQ=255)

                                                                                                                                              aTAGCCTGGGCGCAGAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcg     <  2:1890921/151‑1 (MQ=255)

                                                                                                                                              aTAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcg     <  1:4141509/151‑1 (MQ=255)

                                                                                                                                               tAGCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCAcgc    >  1:5202192/1‑151 (MQ=255)

                                                                                                                                                 gCCTGGGCGCAAAGCGCGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGTGTAGCGCACCGGCAGGATAGGCACGCGt  <  1:923484/151‑1 (MQ=255)

CGGTGCTCTGGCCGGACAGGTAGCGCTCACGGCGGTGGTAGTCCTCCAGCCCTTGGTAGCTCAGCTCTTTGTAGAGGCCCGCGCCGACGTGCTCATGAATGACCAGCTTTTCGCCCTGAGGGCCTTTCATGAACACGTAGACATAGCCCGGGCGCAAAGCACGGAGGGTGTAGGCCGAGTGCTGCAGCGTGGGGAAGCCTTGTTCCAGGTTGAAGCCTGCATCGGCATAGCGGCAGGCGGGGGCGTCGCCGGTGCGGGGCACGATGGCGTAGCGCACCGGCAGGATAGGCACGCGT  >  CP009974/4541488‑4541783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`