Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,543,833	C→G	S430S (TCG→TCC)	RPPX_20290 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,543,833	0	C	G	100.0%	66.0 / NA	42	S430S (TCG→TCC)	RPPX_20290	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/24);  total (18/24)

CTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGT  >  CP009974/4543685‑4543860                                                                                                                                                     |                            ctgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGa                           <  1:4201664/151‑1 (MQ=11)  tgccgctgcGCCAATCACCCCGACCGCAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAg                          <  2:1087156/151‑1 (MQ=11)  tgccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAg                          <  2:1084922/151‑1 (MQ=11)  ggccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAg                          <  2:1085957/150‑1 (MQ=11)   gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                         <  2:4606156/151‑1 (MQ=11)   gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                         >  2:2036496/1‑151 (MQ=11)   gccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAgg                         <  2:1598646/151‑1 (MQ=11)    ccgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGt                        <  1:2895743/151‑1 (MQ=11)     cgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTg                       >  1:5318403/1‑151 (MQ=11)     cgctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTg                       <  1:3498502/151‑1 (MQ=11)      gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      >  1:203541/1‑151 (MQ=11)      gctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGa                      >  2:3185059/1‑151 (MQ=11)       ctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAg                     <  2:5024060/151‑1 (MQ=11)       ctgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGATTAAACCACCACCACCGCCGGAgtagac                     >  2:1818773/1‑146 (MQ=11)        tgcGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGc                    <  1:3030596/151‑1 (MQ=11)         gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCa                   <  2:1297528/151‑1 (MQ=11)         gcgcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCa                   >  1:1943515/1‑151 (MQ=11)          cgcCAGTCACCCCGACCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCaa                  <  2:508767/151‑1 (MQ=11)          cgcCAATCACACCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCaa                  <  2:520217/151‑1 (MQ=11)           gcCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAc                 >  1:2151339/1‑151 (MQ=11)             cAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAccc               <  2:2103479/151‑1 (MQ=11)             cAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAAccc               <  1:3772385/151‑1 (MQ=11)              aaTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCa              >  2:179274/1‑151 (MQ=11)              aaTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCa              >  1:2800622/1‑151 (MQ=11)              aaTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCa              <  1:1465870/151‑1 (MQ=11)               aTCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCaa             <  2:2955602/151‑1 (MQ=11)                tCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAg            <  1:995837/151‑1 (MQ=11)                  aCCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGa          >  2:3179921/1‑151 (MQ=11)                   ccccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGaa         <  1:2362189/151‑1 (MQ=11)                   ccccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGaa         <  1:573563/151‑1 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCGCAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg        >  1:3189473/1‑151 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg        <  2:2726247/151‑1 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg        >  1:771956/1‑151 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg        <  1:4927949/151‑1 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg        >  1:771955/1‑151 (MQ=11)                    cccGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAg        >  1:2726247/1‑151 (MQ=11)                       gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCGATGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGACGTTg     >  2:5213516/1‑151 (MQ=11)                       gAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGCGAGCAACCCAAGGAAGTTg     >  2:3893688/1‑151 (MQ=11)                        aaCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTgg    <  2:256064/151‑1 (MQ=11)                        aaCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCAACCCAAGGAAGTTgg    <  1:3739740/151‑1 (MQ=11)                        aaCACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGGTTATACCACCACCACCGCCGGAGGTGAGCCACGCAAGGCAGTTgg    >  2:636028/1‑151 (MQ=11)                          cacaGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAGGCCGTGTTGAGCGATAAGATGTTGAACCACAGCATCCCTGCGTTTAAACCACCACCACCGCCGGAGGTGAGCGACCCCAGGAAGTTgggg  >  2:2813721/1‑150 (MQ=11)                                                                                                                                                     |                            CTGCCGCTGCGCCAATCACCCCGAACACAGATGAAGCGAACCCCAGGGTGTATTCCGGTGCGTCGCTCTTCTGCAGGCTGTTGTAAGCCGTCTTGAGCGATAGGATGTTGAACCACAGCATCCCCGCGTTTAAACCACCACCACCGCCCGAGGTGAGCAACCCAAGGAAGTTGGGT  >  CP009974/4543685‑4543860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑