Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 4,544,682 T→G S147S (TCA→TCC)  RPPX_20290 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099744,544,6820TG99.4% 563.0 / ‑4.1 177S147S (TCA→TCC) RPPX_20290hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (96/80);  total (97/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 177 total aligned reads displayed.

CCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGG  >  CP009974/4544601‑4544829
                                                                                 |                                                                                                                                                   
ccGGTATTGGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAg                                                                                <  2:4202974/151‑1 (MQ=2)
  ggTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggg                                                                              <  1:1617762/151‑1 (MQ=14)
   gTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgggg                                                                             <  1:2595865/151‑1 (MQ=12)
   gTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAgggg                                                                             <  1:3080379/151‑1 (MQ=12)
    tATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAggggg                                                                            <  2:790241/151‑1 (MQ=16)
     aTTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGc                                                                           <  2:333293/151‑1 (MQ=18)
       ttGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCAt                                                                         <  1:1665301/151‑1 (MQ=19)
       ttGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCAt                                                                         >  1:4229939/1‑151 (MQ=255)
        tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        <  1:2120279/151‑1 (MQ=255)
        tGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATc                                                                        >  1:2262916/1‑151 (MQ=19)
         gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  1:1288433/151‑1 (MQ=255)
         gTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATcc                                                                       <  2:561235/151‑1 (MQ=255)
          tCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccg                                                                      >  2:1884916/1‑151 (MQ=19)
          tCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccg                                                                      <  1:2275309/151‑1 (MQ=19)
           cGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgc                                                                     <  1:2917967/151‑1 (MQ=255)
             aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                   <  1:431571/151‑1 (MQ=255)
             aCATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATccgccg                                                                   >  1:5156086/1‑151 (MQ=255)
               aTCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCggg                                                                 >  2:4818070/1‑151 (MQ=255)
                tCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGt                                                                <  1:3612032/151‑1 (MQ=255)
                ccGAGGTGCTGGGCCGGGCTGGCGCCATTGGGGCAGTAGTGAGCGGGGGTGAGGGCCTTGAAATGGGACTCGGCGGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGt                                                                <  2:3778208/150‑1 (MQ=11)
                 cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               <  2:3035466/151‑1 (MQ=255)
                 cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               <  1:3016673/151‑1 (MQ=255)
                 cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               >  1:2014721/1‑151 (MQ=16)
                 cGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTa                                                               >  1:1204700/1‑151 (MQ=19)
                  gAGGCGCTGTGCCAGGCGCGCGCCATGGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGGGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATGCGCCGGGGAc                                                              >  2:543868/1‑151 (MQ=11)
                    ggCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAg                                                            >  2:1573569/1‑151 (MQ=255)
                      cgcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGc                                                          >  1:107957/1‑151 (MQ=255)
                       gcTGTGCCAGGCGCGCGCCATTGGGGGCGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCCGCGTGAAGTGCCCAGCATGAACGCCGCCAGGTCCGGCGAGGGGGCGTCCGCCGGGTACAGGct                                                         >  2:236288/1‑151 (MQ=11)
                       gcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGct                                                         >  1:3175987/1‑151 (MQ=255)
                       gcTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCACTTCCGGCGAGGGGGCCGCCGCCGGGTACCGGcc                                                         >  2:4658906/1‑150 (MQ=11)
                         tgtgCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGctct                                                       >  1:2391062/1‑151 (MQ=255)
                            gCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCa                                                    >  2:5111312/1‑151 (MQ=255)
                            gCCAGGCGCGCGCCAGTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGGCTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCa                                                    <  2:1366821/151‑1 (MQ=255)
                              cAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGc                                                  <  1:4044571/151‑1 (MQ=255)
                                ggCGCGCGCCATTGGGGGAGTGATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGt                                                <  1:494860/151‑1 (MQ=255)
                                ggCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGGGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGt                                                <  1:4430089/151‑1 (MQ=255)
                                 gcgcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTc                                               >  1:2280628/1‑151 (MQ=255)
                                   gcgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCtt                                             >  1:671109/1‑151 (MQ=255)
                                    cgcgcgATTGGGGGAGTACTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTAAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCttn                                            <  1:4942317/151‑2 (MQ=11)
                                    cgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTg                                            <  2:716271/151‑1 (MQ=255)
                                    cgcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTg                                            <  1:3834156/151‑1 (MQ=255)
                                     gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                           >  2:3471758/1‑151 (MQ=255)
                                     gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGt                                           <  1:1784978/151‑1 (MQ=255)
                                     gcgcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGCGAGGGGGCATCCGCCGGGTACCGGCTCTCCAGCGCCCTGt                                           >  1:4774579/1‑151 (MQ=11)
                                       gcCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGGACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTGCAGGCGCTCCAGCGGCGTGTTc                                         >  2:4697148/1‑151 (MQ=11)
                                        ccATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTc                                               <  2:2634972/144‑1 (MQ=255)
                                         cATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcag                                       >  1:2991253/1‑151 (MQ=255)
                                          aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                      <  1:3337196/151‑1 (MQ=255)
                                          aTTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagc                                      >  2:4842021/1‑151 (MQ=255)
                                           ttGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagca                                     >  1:2614233/1‑151 (MQ=255)
                                            tGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTcagcag                                    <  1:4876255/151‑1 (MQ=255)
                                              ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                  <  1:4681675/151‑1 (MQ=255)
                                              ggggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAgcagca                                  <  2:4495093/151‑1 (MQ=255)
                                               gggAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAAGGGGCATCCGCCGGGTACAGGCTCTCCAGCGCCGTCTTCAGCAGCAt                                 >  2:43303/1‑151 (MQ=255)
                                               gggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCAt                                 >  2:908166/1‑151 (MQ=255)
                                               gggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCAt                                 <  1:4025257/151‑1 (MQ=255)
                                                ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                <  1:5363420/151‑1 (MQ=255)
                                                ggAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATg                                >  1:619664/1‑151 (MQ=255)
                                                 gAGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATgg                               >  2:2154084/1‑151 (MQ=255)
                                                  aGTAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGc                              <  1:1232967/151‑1 (MQ=255)
                                                   gTAATCGTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCg                             <  2:5348252/151‑1 (MQ=255)
                                                    tAATCTTCGTGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            <  2:1808966/151‑1 (MQ=255)
                                                    tAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            >  1:1143702/1‑151 (MQ=255)
                                                    tAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            <  2:630189/151‑1 (MQ=255)
                                                    tAATCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGa                            <  1:1420588/151‑1 (MQ=255)
                                                     aaTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGCGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGCCTTGCTCACCAGCATGGCGAc                           >  2:4938358/1‑151 (MQ=11)
                                                      aTCTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGAcc                          >  2:3945286/1‑151 (MQ=255)
                                                        cTTCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTCCAGCAGCATGGCGACCAg                        >  2:3659728/1‑151 (MQ=255)
                                                        cTTCATGGGGGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAg                        <  1:2690320/151‑1 (MQ=255)
                                                         ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                       <  2:4545684/151‑1 (MQ=255)
                                                         ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCACGCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAgg                       >  2:5216192/1‑151 (MQ=255)
                                                         ttCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGGGGGGGGGCATCCGCCGGGTACCGGCTCTCCGGCGCCCTGCGCAGCAGCATGGAGAcccgc                       >  2:4087327/1‑148 (MQ=11)
                                                          tCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGt                      >  2:2236527/1‑151 (MQ=255)
                                                          tCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGt                      >  1:2924399/1‑151 (MQ=255)
                                                          tCATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGt                      <  1:3349884/151‑1 (MQ=255)
                                                           cATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGtt                     <  1:1076372/151‑1 (MQ=255)
                                                           cATGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGtt                     >  1:467106/1‑151 (MQ=255)
                                                             tGGGTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGc                   >  2:3121078/1‑151 (MQ=255)
                                                                gTGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCTGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGAt                >  2:3380712/1‑151 (MQ=255)
                                                                 tGAGGGCCTGGAAATGGGGCGCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATg               <  2:1856308/151‑1 (MQ=255)
                                                                 tGAGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATg               >  2:1230553/1‑151 (MQ=255)
                                                                 tGAGGGCCTGGAAAGGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATg               <  2:3044400/151‑1 (MQ=255)
                                                                   ggggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt             <  2:2051039/150‑1 (MQ=255)
                                                                   aGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGt             >  2:1863317/1‑151 (MQ=255)
                                                                   aGGGCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGGTCCGGTGAGGGGGCATCCGCCGCGTAGAGGCTCTCCACCGCCTGGTTCAGCAGCATGGCGACCAGGGTGCGATggg             >  2:5204271/1‑150 (MQ=11)
                                                                     ggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGa           >  2:2295271/1‑151 (MQ=255)
                                                                     ggCCTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGa           >  2:1222552/1‑151 (MQ=255)
                                                                       ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGCTGGTGcaa         >  2:638450/1‑151 (MQ=255)
                                                                       ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa         <  1:2507614/151‑1 (MQ=255)
                                                                       ccTGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCGGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCATCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGaaa         >  2:4146902/1‑151 (MQ=255)
                                                                         tGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGt       >  2:226061/1‑151 (MQ=255)
                                                                         tGGAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGt       >  1:2635472/1‑151 (MQ=255)
                                                                          ggAAATGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTc      <  2:2505918/151‑1 (MQ=255)
                                                                            aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc    >  1:96493/1‑151 (MQ=255)
                                                                            aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc    >  1:3017117/1‑151 (MQ=255)
                                                                            aaaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgc    >  2:1194712/1‑151 (MQ=255)
                                                                             aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgcg   <  1:2542922/151‑1 (MQ=255)
                                                                             aaTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTcgcg   <  2:2370158/151‑1 (MQ=255)
                                                                              aTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCgg  >  1:900650/1‑151 (MQ=255)
                                                                              aTGGGACTCGGCCGGCGACAGGGCAGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCgg  >  1:2541652/1‑151 (MQ=255)
                                                                                 |                                                                                                                                                   
CCGGTATTTGTCGACATCGAGGCGCTGTGCCAGGCGCGCGCCATTGGGGGAGTAATCTTCATGGGTGAGGGCCTGGAAATGTGACTCGGCCGGCGACAGGGCGGGCCCCCTGCGTGAAGTGCCCAGCCTGAACGCGGCCAGTTCCGGTGAGGGGGCATCCGCCGGGTACAGGCTCTCCAGCGTCTTGTTCAGCAGCATGGCGACCAGGTTGCGATGGTGAAAGTCGCGG  >  CP009974/4544601‑4544829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑