Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,631,683	G→A	intergenic (+245/‑585)	emrB → / → RPPX_20645	multidrug resistance protein B/oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,631,683	0	G	A	100.0%	157.5 / NA	53	intergenic (+245/‑585)	emrB/RPPX_20645	multidrug resistance protein B/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/35);  total (18/35)

GAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631535‑4631712                                                                                                                                                     |                              gaggCCGTGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATagg                             <  1:2672367/151‑1 (MQ=255) gaggCCGAGCGGAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATagg                             <  1:2023182/151‑1 (MQ=255) gaggCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATagg                             <  1:2021686/151‑1 (MQ=255)  aggCCGAGCGGAGGGCTTGCGAAGGGGGGTGACGGGCATGGGTGCCCGTCAAGCGCGGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            <  1:1188930/151‑1 (MQ=255)  aggCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGa                            >  1:272397/1‑151 (MQ=255)   ggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGCGGCCCCATGGATGGGGCCCGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAg                           <  2:4543194/151‑1 (MQ=255)   ggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAg                           <  1:1150523/151‑1 (MQ=255)    gCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCATGCAGCGCGTACTCCCGGGAGCAAGACCGTAGAGAGGCGACCCCGGAGCGTACCGCCGGGCGCGGATGATAGGAgc                          >  2:3076490/1‑151 (MQ=11)      cGAGTGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcca                        <  1:2293292/151‑3 (MQ=255)      cGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgcca                        <  1:2293758/151‑3 (MQ=255)       gAGCGTAGGGCTTGCGAAGGGAGGTGTCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccag                       >  2:3179702/1‑148 (MQ=255)       gAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccag                       <  1:5169521/151‑4 (MQ=255)        ggcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGAGGCCCGGCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccagc                      <  1:962322/150‑5 (MQ=11)        ggCGTAGGGCTGGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccagc                      <  1:2414899/150‑5 (MQ=255)        aGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccagc                      >  2:2240395/1‑147 (MQ=255)        aGCGTAGGGCTTGCGAAGGGAGGTGACGGGCAGGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAgccagc                      <  1:2331084/151‑5 (MQ=255)         gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  1:4834815/151‑1 (MQ=255)         gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  1:4834647/151‑1 (MQ=255)         gCGTAGGGCTTGCGAAGGGAGGTGGCGGGCATGGAAGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  2:2420677/151‑1 (MQ=255)          cgcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCgc                   >  1:5208981/4‑150 (MQ=255)          cgcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   >  1:1558017/4‑151 (MQ=255)          cGTAGGGCTTGCGAAGGGGGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGACTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   <  1:1585364/151‑1 (MQ=255)          cGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGt                   >  1:693846/1‑151 (MQ=255)           gcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  2:601439/149‑1 (MQ=255)           gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGGTGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCGTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  2:3452462/151‑1 (MQ=255)           gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  1:5432623/151‑1 (MQ=255)            tggggCTTGCGAAGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCGGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 <  2:2266927/149‑1 (MQ=11)            tAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 <  2:2804909/151‑1 (MQ=255)             ggggCTGGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:1816461/150‑1 (MQ=255)             aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                >  1:1306851/1‑151 (MQ=255)             aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:1070480/151‑1 (MQ=255)             aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGgttt                >  1:3692283/1‑151 (MQ=255)              gggCTTGCGAGGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  2:2206600/151‑1 (MQ=255)               ggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttgttt              >  2:3312452/1‑151 (MQ=255)                gCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg             >  1:3373748/1‑151 (MQ=255)                gCTTGCGAAGGGAGGGCGCGGGTATGGAGGCCCGTCAAGCGCTGAGGCCGTATGGATGGGGCCTGCAGCGCGTCTGCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg             <  2:2908345/151‑1 (MQ=11)                  ttGCGAAGGGAGGTGACGGGCAGGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  1:3801612/151‑1 (MQ=255)                  ttGCGAAGGGAGGGGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  2:1997409/151‑1 (MQ=255)                   tGCGAAGGGGGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCGGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  1:5364695/151‑1 (MQ=255)                   tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  2:5331421/151‑1 (MQ=16)                   tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          >  1:2044138/1‑151 (MQ=255)                   tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  1:1401007/151‑1 (MQ=255)                   ggCGAGGGGAGGTGGCGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  2:5332567/150‑1 (MQ=11)                    gggaggggAGGTGGCGGGCAGGGAGGCCCGTCAAGCGCGGAGGACAGATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  2:2402208/146‑1 (MQ=11)                    gCGAAGGGAGGTGGCGGGGATGGGTGCCCGTCAAGCGCTGAGGCCGCATGGTGGGGGCCTGAAGCGCGTACTCACGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  2:1963037/151‑1 (MQ=11)                    gCGAAGGGAGGTGACGTGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTa         <  1:4201621/151‑1 (MQ=255)                     cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  2:3013412/151‑1 (MQ=255)                      gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  2:1768076/1‑151 (MQ=16)                      gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:4316626/1‑151 (MQ=16)                      gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:4318364/1‑151 (MQ=16)                       aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:4320790/151‑1 (MQ=12)                        aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     >  1:4948137/1‑151 (MQ=9)                        aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     <  2:1207079/151‑1 (MQ=9)                        aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     >  1:645074/1‑151 (MQ=9)                          ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   >  1:1271147/1‑151 (MQ=9)                           gAGGTGACGGGCATGGAGGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  2:4788604/151‑1 (MQ=2)                                                                                                                                                     |                              GAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631535‑4631712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑