Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,281 A→T Y68F (TAC→TTC)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,2810AT99.5% 772.2 / NA 211Y68F (TAC→TTC) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base T (95/114);  minor base G (0/1);  total (95/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 211 total aligned reads displayed.

AGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACA  >  CP009974/5007133‑5007427
                                                                                                                                                    |                                                                                                                                                  
 gCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTccc                                                                                                                                                 <  1:2644205/151‑1 (MQ=255)
   gTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCt                                                                                                                                               <  1:1885774/151‑1 (MQ=255)
    gcGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGTAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCtt                                                                                                                                              <  1:2032169/150‑1 (MQ=255)
      gATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCa                                                                                                                                            <  2:4424825/151‑1 (MQ=255)
         gCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCCCGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCg                                                                                                                                         <  2:4746832/151‑1 (MQ=255)
         gCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCg                                                                                                                                         <  1:4642306/151‑1 (MQ=255)
         gCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCg                                                                                                                                         <  1:3445477/151‑1 (MQ=255)
          cccTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCgg                                                                                                                                        >  1:1380979/1‑151 (MQ=255)
          cccTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCgg                                                                                                                                        <  1:3334943/151‑1 (MQ=255)
           ccTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGc                                                                                                                                       >  1:936760/1‑151 (MQ=255)
           ccTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGc                                                                                                                                       <  2:3089384/151‑1 (MQ=255)
                gcCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGaa                                                                                                                                  >  2:5285169/1‑151 (MQ=255)
                 gcggtaCCGGCTGGAGCGTTTGTGCTGGAGCGTGAGGCGCGCCTACGACAGTGTGCCGTTGTACCGCTGGCGCTTTGCCGAGTACGGTACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAAc                                                                                                                                 <  2:1236389/146‑1 (MQ=255)
                  cAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACg                                                                                                                                >  1:135735/1‑151 (MQ=255)
                    gCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGac                                                                                                                              <  1:3053875/151‑2 (MQ=255)
                      aCCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCt                                                                                                                            <  1:37626/151‑1 (MQ=255)
                      aCCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCt                                                                                                                            <  1:38428/151‑1 (MQ=255)
                       ccAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTg                                                                                                                           >  1:2223830/1‑151 (MQ=255)
                        cAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTgc                                                                                                                          <  1:4129929/151‑1 (MQ=255)
                        cAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTgc                                                                                                                          <  1:476742/151‑1 (MQ=255)
                           cTGGAGCGGGTGCGGTGGAGCCTGAAGCACGCCTACGACAAAGAGCCGTTGTACCGCCAGCGCTTTGACGAGTAAGGCACCAACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGGTCCACTTCACCGGCAAGAACGACATGcgcg                                                                                                                       <  2:666248/151‑1 (MQ=255)
                             ggaacctCTGCACTGGCGCCTGACGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGAc                                                                                                                     <  1:62995/145‑1 (MQ=255)
                              gAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACa                                                                                                                    >  2:2745834/1‑151 (MQ=255)
                              gAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGCCAAGAACGACCTGCGCGCCa                                                                                                                    >  2:1916484/1‑151 (MQ=255)
                               aGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACaa                                                                                                                   >  2:75863/1‑151 (MQ=255)
                                     tGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAccc                                                                                                             <  2:1395310/151‑1 (MQ=255)
                                         cTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCtac                                                                                                         <  2:3952/151‑3 (MQ=255)
                                            gAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACGGGCAAGAACGACCTGCGCGACAACTACCCCTACGGc                                                                                                      >  2:3392376/1‑151 (MQ=255)
                                             aGCCTGAAGCCCGCCTACGACAATGTGCCGTTGTGCCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCa                                                                                                     <  2:3028558/151‑1 (MQ=255)
                                             aGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCa                                                                                                     <  2:2921217/151‑1 (MQ=255)
                                              gCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCAt                                                                                                    <  1:2341832/151‑1 (MQ=255)
                                                     gCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGcc                                                                                             <  2:2423438/151‑1 (MQ=255)
                                                     gCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGcc                                                                                             <  1:340212/151‑1 (MQ=255)
                                                     gCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGcc                                                                                             >  1:2283495/1‑151 (MQ=255)
                                                      cACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCg                                                                                            >  1:5237085/1‑151 (MQ=255)
                                                          ccTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGcc                                                                                        <  1:3639650/151‑1 (MQ=255)
                                                          ccTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGcc                                                                                        <  1:5307719/151‑1 (MQ=255)
                                                           cTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGccc                                                                                       >  2:1545773/1‑151 (MQ=255)
                                                            tACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGcccc                                                                                      >  1:3171296/1‑151 (MQ=255)
                                                            tACGACAATGTGCCGGTGTAGCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCTAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACGACTACCCCTACGGCATGTTTGCCGTGcccc                                                                                      <  2:62965/151‑1 (MQ=255)
                                                             aCGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCa                                                                                     <  1:21188/151‑1 (MQ=255)
                                                                aCAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACTACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGga                                                                                  >  2:3373588/1‑151 (MQ=255)
                                                                 cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCTCCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                 >  2:4989852/1‑151 (MQ=255)
                                                                  aaTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaag                                                                                >  2:702205/1‑151 (MQ=255)
                                                                   aTGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaaga                                                                               <  1:2544843/151‑1 (MQ=255)
                                                                     gtgCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGaagaag                                                                             <  1:1557268/151‑1 (MQ=255)
                                                                       gCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTTCCGTGCCCCAGCAAGAAgtg                                                                           >  2:83232/1‑151 (MQ=255)
                                                                        ccGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtgg                                                                          >  2:2738701/1‑151 (MQ=255)
                                                                         cGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggt                                                                         >  2:4329568/1‑151 (MQ=255)
                                                                         cGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggt                                                                         <  1:280339/151‑1 (MQ=255)
                                                                         cGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggt                                                                         <  1:280175/151‑1 (MQ=255)
                                                                          gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGCCCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                        >  2:5152234/1‑151 (MQ=255)
                                                                          gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                        >  1:3191420/1‑151 (MQ=255)
                                                                          gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                        >  1:108067/1‑151 (MQ=255)
                                                                           ttGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgc                                                                       <  1:1545773/151‑1 (MQ=255)
                                                                             gTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgcgc                                                                     >  1:3800347/1‑151 (MQ=255)
                                                                              tACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGcc                                                                    <  2:2205382/151‑1 (MQ=255)
                                                                               aCCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCt                                                                   >  2:1580362/1‑151 (MQ=255)
                                                                                ccGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTg                                                                  <  1:4804201/151‑1 (MQ=255)
                                                                                   ccAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAt                                                               <  2:2519018/151‑1 (MQ=255)
                                                                                   ccAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAt                                                               <  1:4989852/151‑1 (MQ=255)
                                                                                   ccAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAt                                                               <  2:126968/151‑1 (MQ=255)
                                                                                        gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTc                                                          <  1:1315319/151‑1 (MQ=255)
                                                                                          tttGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCa                                                        >  1:1592272/1‑151 (MQ=255)
                                                                                          tttGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCa                                                        >  2:489330/1‑151 (MQ=255)
                                                                                            tGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAgt                                                      <  2:936760/151‑2 (MQ=255)
                                                                                               cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                   >  2:1890998/1‑151 (MQ=255)
                                                                                               cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                   >  2:2360255/1‑151 (MQ=255)
                                                                                               cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                   >  1:3545453/1‑151 (MQ=255)
                                                                                               cGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGc                                                   <  1:3959136/151‑1 (MQ=255)
                                                                                                    aCGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcaccac                                              >  1:1844372/1‑151 (MQ=255)
                                                                                                       gCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCgg                                           <  2:3545453/151‑1 (MQ=255)
                                                                                                       gCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCgg                                           <  1:3726518/151‑1 (MQ=255)
                                                                                                           ccacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAg                                       >  1:4895853/1‑151 (MQ=255)
                                                                                                            cacccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGc                                      >  1:1675789/1‑151 (MQ=255)
                                                                                                               ccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGa                                   >  1:1197483/1‑151 (MQ=255)
                                                                                                               ccTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGa                                   <  1:2686426/151‑1 (MQ=255)
                                                                                                                cTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGAc                                  <  1:5153552/151‑1 (MQ=255)
                                                                                                                  gatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACgg                                >  2:5359320/1‑151 (MQ=255)
                                                                                                                     gaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtgg                             >  1:3461854/1‑151 (MQ=255)
                                                                                                                      aCCTCAAGTGCCTGGACGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggt                            <  2:3297163/151‑1 (MQ=255)
                                                                                                                       ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                           <  1:1220698/151‑1 (MQ=255)
                                                                                                                       ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                           <  2:4115447/151‑1 (MQ=255)
                                                                                                                        cTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCg                          <  2:1887571/151‑1 (MQ=255)
                                                                                                                         tCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCgg                         <  1:3317781/151‑1 (MQ=255)
                                                                                                                          cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                        <  1:3429592/151‑1 (MQ=255)
                                                                                                                            aGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTa                      <  1:3363597/151‑1 (MQ=255)
                                                                                                                             gTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTac                     <  1:5367355/151‑1 (MQ=255)
                                                                                                                             gTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTac                     >  2:1308605/1‑151 (MQ=255)
                                                                                                                                  tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCg                                    >  1:1042445/1‑131 (MQ=255)
                                                                                                                                  tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTCCACCCa                >  2:2311306/1‑151 (MQ=255)
                                                                                                                                    gAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGa              >  1:1985959/1‑151 (MQ=255)
                                                                                                                                    gAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGa              >  2:5373725/1‑151 (MQ=255)
                                                                                                                                       gACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACg           <  1:4356133/151‑1 (MQ=255)
                                                                                                                                         ccTGGCGACGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGat         <  2:3267667/151‑1 (MQ=255)
                                                                                                                                          cTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGata        <  1:2929458/151‑1 (MQ=255)
                                                                                                                                            ggCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATc      >  1:4030749/1‑151 (MQ=255)
                                                                                                                                            ggCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATc      >  1:84196/1‑151 (MQ=255)
                                                                                                                                               gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGac   >  1:3590171/1‑151 (MQ=255)
                                                                                                                                               gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGac   <  2:233656/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACA  >  CP009974/5007133‑5007427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑