Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,105,351 T→C P167P (CCA→CCG)  RPPX_22650 ← benzene 1,2‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,105,3510TC83.5% 137.6 / 39.1 103P167P (CCA→CCG) RPPX_22650benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (8/9);  new base C (38/48);  total (46/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 104 total aligned reads displayed.

GTGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGC  >  CP009974/5105201‑5105496
                                                                                                                                                      |                                                                                                                                                 
atgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGt                                                                                                                                                   >  1:1351653/5‑151 (MQ=1)
 tgctAGCCGTGGGCGCCGTGCTCGGCGGTCAGCGTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTg                                                                                                                                                  <  2:5319793/148‑1 (MQ=1)
 tgatAGCCGTCGGCGCCGTTCTCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTg                                                                                                                                                  <  2:2099453/148‑1 (MQ=1)
                   ttctCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCa                                                                                                                                >  2:668889/4‑151 (MQ=1)
                   ttctCGGCGGTCAGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCa                                                                                                                                <  2:3818708/148‑1 (MQ=1)
                   ttctCGGCGGTCAGCTTCCAGTCACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTTGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCa                                                                                                                                <  2:5234826/148‑1 (MQ=1)
                            gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                       <  1:2997544/148‑1 (MQ=1)
                            gtcaGCTTCCAGTTACCTTCGTAGATGTAGGAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCaa                                                                                                                       >  2:2566899/4‑151 (MQ=1)
                                       gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                            <  2:2449157/151‑1 (MQ=1)
                                       gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                            <  2:2447120/151‑1 (MQ=1)
                                                       taggAGCTAGCTCCACGCAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATc                                                                                            <  2:1263687/148‑1 (MQ=1)
                                                                 ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTTCCACCtt                                                                                  >  2:1941651/6‑151 (MQ=1)
                                                                 ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCtt                                                                                  >  1:2652791/6‑151 (MQ=1)
                                                                  tccacgcAGCACTTCCAGGCGGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTg                                                                                 <  2:3053862/147‑1 (MQ=1)
                                                                  tccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTg                                                                                 >  1:3908769/5‑151 (MQ=1)
                                                                   ccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTgg                                                                                >  1:3929407/4‑151 (MQ=1)
                                                                          ggacgtCCGGGCCTCCAGGCGACTGATCGACGATCATGGCGATGACCTTCGCCGCCCCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTAAGGATGAAGAAAAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTc                                                                         <  2:2219530/146‑1 (MQ=0)
                                                                          gcgctgccAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTc                                                                         <  2:3610142/145‑1 (MQ=1)
                                                                                       gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCg                                                            <  2:3314535/147‑1 (MQ=1)
                                                                                        tccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGGTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGACTCGTGCCACCTTGGTCAGGGCGTGGGAGCCGTCGc                                                           >  2:5211982/4‑151 (MQ=1)
                                                                                                 tGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                  <  2:1751245/151‑1 (MQ=11)
                                                                                                 tGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                  <  1:4700014/151‑1 (MQ=11)
                                                                                                 tGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                  <  1:3954260/151‑1 (MQ=11)
                                                                                                  gATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                 >  1:2442686/1‑151 (MQ=11)
                                                                                                   aTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTg                                                <  1:812696/151‑1 (MQ=11)
                                                                                                    tCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGt                                               <  1:817078/151‑1 (MQ=11)
                                                                                                     cgacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTc                                              >  2:3141291/1‑151 (MQ=11)
                                                                                                      gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                             <  1:641181/151‑1 (MQ=11)
                                                                                                      gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                             <  1:5247457/151‑1 (MQ=11)
                                                                                                      gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                             >  1:3550411/1‑151 (MQ=11)
                                                                                                       acgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgg                                            >  1:2776416/1‑151 (MQ=11)
                                                                                                       acgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCACGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgg                                            >  2:5190524/1‑151 (MQ=11)
                                                                                                        cgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCggg                                           <  1:128520/151‑1 (MQ=11)
                                                                                                         gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgtgg                                          >  1:410258/1‑151 (MQ=11)
                                                                                                         gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgggg                                          >  1:5192008/1‑151 (MQ=11)
                                                                                                         gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGGACGACTCACAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgggg                                          >  2:3823158/1‑151 (MQ=11)
                                                                                                          aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                         <  1:1099162/151‑1 (MQ=11)
                                                                                                          aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                         >  1:4082978/1‑151 (MQ=11)
                                                                                                          aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                         <  2:1869471/151‑1 (MQ=11)
                                                                                                              tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                     <  1:4623695/151‑1 (MQ=11)
                                                                                                              tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                     <  1:4528427/151‑1 (MQ=11)
                                                                                                              tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                     <  1:2923602/151‑1 (MQ=11)
                                                                                                               gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                    <  1:4637503/151‑1 (MQ=11)
                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                   >  1:289304/1‑151 (MQ=11)
                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                   <  1:495812/151‑1 (MQ=11)
                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                   >  2:1990908/1‑151 (MQ=11)
                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                   <  1:2537538/151‑1 (MQ=11)
                                                                                                                tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCAgg                                   >  1:287132/1‑151 (MQ=11)
                                                                                                                  gatgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCg                                 >  1:4295127/1‑151 (MQ=11)
                                                                                                                    tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                               <  1:2795052/151‑1 (MQ=11)
                                                                                                                    tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                               <  1:3400664/151‑1 (MQ=11)
                                                                                                                    tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                               >  1:3592006/1‑151 (MQ=11)
                                                                                                                      atCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGc                             <  1:4534713/151‑1 (MQ=11)
                                                                                                                       tCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCt                            <  2:3101252/151‑1 (MQ=11)
                                                                                                                        cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                           >  2:2594966/1‑151 (MQ=11)
                                                                                                                        cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                           <  1:2678590/151‑1 (MQ=11)
                                                                                                                         ttCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgg                          <  1:1785677/151‑1 (MQ=11)
                                                                                                                          tCGCCGACTCGCCCAGGTGCTCGACCAGTGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTggg                         >  1:5424821/1‑151 (MQ=11)
                                                                                                                          tCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTggg                         >  1:1078796/1‑151 (MQ=11)
                                                                                                                          tCGCCGACGCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTggg                         <  1:240061/151‑1 (MQ=11)
                                                                                                                           cGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAGAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgggg                        <  2:3295461/151‑1 (MQ=11)
                                                                                                                           cGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGTGCCGTCGCAGTTGAAGCTGTCCGGGTAGCCGGCGTTGCTgggg                        >  2:1514115/1‑151 (MQ=11)
                                                                                                                            gCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGt                       >  1:4866563/1‑151 (MQ=11)
                                                                                                                              cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                     <  1:1682256/151‑1 (MQ=11)
                                                                                                                              cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                     >  1:511626/1‑151 (MQ=11)
                                                                                                                              cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                     >  1:2684962/1‑151 (MQ=11)
                                                                                                                              cGACTCGCCCAGCTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                     <  1:3817212/151‑1 (MQ=11)
                                                                                                                               gACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCTAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCtt                    <  2:5223292/151‑1 (MQ=11)
                                                                                                                                aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                  <  1:3117428/120‑1 (MQ=11)
                                                                                                                                aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                  >  2:3117428/1‑120 (MQ=11)
                                                                                                                                aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                   >  1:75877/1‑151 (MQ=11)
                                                                                                                                aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                   >  2:610384/1‑151 (MQ=11)
                                                                                                                                aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCACGTCGTGGGAGCCGACGCAGTTGAAGCAGACGGGGTAGCCGACGTTGCTAGGGACtat                   >  2:610324/1‑149 (MQ=11)
                                                                                                                                 cTCGCCCAGGTGCTCGCCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                  <  2:483519/151‑1 (MQ=11)
                                                                                                                                 cTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                  >  2:3616139/1‑151 (MQ=11)
                                                                                                                                  ccGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCGCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCa                 <  2:5177899/150‑1 (MQ=11)
                                                                                                                                   cGCCCGGGTGCTCGCCCAGCGGCTTCACATCGGCGTTCCGGCTGCCGAACAAAAAGCCTCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                <  1:4809361/151‑1 (MQ=11)
                                                                                                                                   cGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                >  2:356838/1‑151 (MQ=11)
                                                                                                                                   cGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                <  2:367888/151‑1 (MQ=11)
                                                                                                                                    gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc               <  1:2829674/151‑1 (MQ=11)
                                                                                                                                    gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc               <  1:2846409/151‑1 (MQ=11)
                                                                                                                                    gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc               <  2:2013951/151‑1 (MQ=11)
                                                                                                                                    gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc               >  1:5245481/1‑151 (MQ=14)
                                                                                                                                     cccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCt              >  1:1023682/1‑151 (MQ=14)
                                                                                                                                      ccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCtt             >  1:2304386/1‑151 (MQ=11)
                                                                                                                                       cAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTc            <  2:4049353/151‑1 (MQ=14)
                                                                                                                                       cAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTc            >  2:2628357/1‑151 (MQ=11)
                                                                                                                                        aGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTca           <  2:3955464/151‑1 (MQ=14)
                                                                                                                                         ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag          >  1:4574808/1‑151 (MQ=14)
                                                                                                                                         ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag          >  2:2115500/1‑151 (MQ=14)
                                                                                                                                         ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag          <  2:2157034/151‑1 (MQ=14)
                                                                                                                                         ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGCTGCTGGGGTCCTTCACCTCcag          >  2:5295321/1‑151 (MQ=11)
                                                                                                                                            gCTCGACCAGCAGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagcag       <  1:1671327/151‑1 (MQ=14)
                                                                                                                                             cTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGt      <  2:1078796/151‑1 (MQ=14)
                                                                                                                                              tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt     >  2:530034/1‑151 (MQ=14)
                                                                                                                                              tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt     >  2:4129790/1‑151 (MQ=14)
                                                                                                                                              tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt     <  1:2821682/151‑1 (MQ=14)
                                                                                                                                               cGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGttt    <  2:4516520/151‑1 (MQ=14)
                                                                                                                                                gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg   >  2:3328693/1‑151 (MQ=14)
                                                                                                                                                 aCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGc  <  1:3215567/151‑1 (MQ=14)
                                                                                                                                                      |                                                                                                                                                 
GTGGTAGCCGTCGGCGCCGTTTTCGGCGGTGAGCTTCCAGTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGC  >  CP009974/5105201‑5105496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑