BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	25,739	A→T	intergenic (+164/‑407)	RPPX_00140 → / → RPPX_00145	MarR family transcriptional regulator/(2Fe‑2S)‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	25,739	0	A	T	96.0%	723.0 / 0.0	231	intergenic (+164/‑407)	RPPX_00140/RPPX_00145	MarR family transcriptional regulator/(2Fe‑2S)‑binding protein
Reads supporting (aligned to +/- strand): ref base A (2/7); new base T (103/113); total (106/125)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

CCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTGGCCAATCTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGATCACCGTTGCCGCCGCC  >  CP009974/25591‑25898

 cgcgGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTccc                                                                                                                                                              <  1:2690224/151‑1 (MQ=255)

  gcgGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCccg                                                                                                                                                             >  2:3351914/1‑151 (MQ=255)

  gcgGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAGGGGCCGGTCCTGCTAGCCAATCTCCTCccg                                                                                                                                                             >  2:1800695/1‑151 (MQ=255)

    ggTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGGCCTGCCAGCCAACCTCCTCccgcc                                                                                                                                                           >  2:2708666/1‑151 (MQ=255)

     gTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCccgccg                                                                                                                                                          <  1:364769/151‑1 (MQ=255)

     gTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCccgccg                                                                                                                                                          <  1:4384831/151‑1 (MQ=255)

       ggCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGtt                                                                                                                                                        <  1:1293761/151‑1 (MQ=255)

       agcagTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGTGCAAGCTCGCTCACATTGGACACTCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGtt                                                                                                                                                        <  2:4406585/147‑1 (MQ=37)

            tCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGATCACCg                                                                                                                                                   >  2:3164828/1‑151 (MQ=255)

             cacaGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGt                                                                                                                                                  <  1:2311481/151‑1 (MQ=255)

              acaGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGta                                                                                                                                                 >  2:5362594/1‑150 (MQ=255)

               caGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTg                                                                                                                                                <  1:4400805/151‑1 (MQ=255)

               caGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTg                                                                                                                                                <  2:3364107/151‑1 (MQ=255)

               caGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTg                                                                                                                                                <  1:902056/151‑1 (MQ=255)

                aGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGTACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTTGCCAATCTCCTCCCGCCGTTCACCATTgc                                                                                                                                               >  2:1698769/1‑151 (MQ=255)

                aGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgc                                                                                                                                               <  1:4456873/151‑1 (MQ=255)

                    aCGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGCTCACCGTTgccgcc                                                                                                                                           >  1:3587951/1‑151 (MQ=255)

                     cgcgGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccgccg                                                                                                                                          <  2:1891395/151‑1 (MQ=255)

                       cgGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTgccgccgcc                                                                                                                                        >  1:767341/1‑151 (MQ=255)

                        ggCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTACCGCCGCCa                                                                                                                                       >  2:28758/1‑151 (MQ=255)

                         gCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGGCACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCAc                                                                                                                                      >  1:4078404/1‑151 (MQ=255)

                          ccTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCAcc                                                                                                                                     >  2:2149016/1‑151 (MQ=255)

                          ccTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCAcc                                                                                                                                     <  2:1998802/151‑1 (MQ=255)

                          ccTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCAAATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCAcc                                                                                                                                     <  1:1023980/151‑1 (MQ=255)

                           cTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCg                                                                                                                                    <  1:786094/151‑1 (MQ=255)

                           cTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCg                                                                                                                                    >  1:2401093/1‑151 (MQ=255)

                            tGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTTCTAGCCAATCTCCTCCCGCCGTTCACCGTTTCCGCCGCCACCGt                                                                                                                                   >  1:413662/1‑151 (MQ=255)

                              agagaTCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCCCCTCCCGCCGTTCACCGTTACCGCCGCCACCGTAt                                                                                                                                 >  2:2498784/1‑151 (MQ=255)

                                agaTCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTc                                                                                                                               <  2:4979146/151‑1 (MQ=255)

                                  aTCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTcgc                                                                                                                             >  1:1915001/1‑151 (MQ=255)

                                  aTCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTcgc                                                                                                                             >  1:2263485/1‑151 (MQ=255)

                                     aaGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCggg                                                                                                                          >  2:1215715/1‑151 (MQ=255)

                                      aGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGt                                                                                                                         <  1:4689991/151‑1 (MQ=255)

                                        cacaTCGCGAGCAAGCGCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTaa                                                                                                                       <  2:4872320/151‑1 (MQ=255)

                                            ccgcgAGAAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAccc                                                                                                                   <  2:702452/150‑1 (MQ=255)

                                             cgcgAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCg                                                                                                                  <  2:3520288/151‑1 (MQ=255)

                                                   cAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCCCCGTTGCCTCCGCCACCGTATTCGCCGGTAACCCCGcccata                                                                                                            >  2:5275388/1‑146 (MQ=18)

                                                    aaGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTac                                                                                                           <  1:3817604/151‑1 (MQ=255)

                                                      gcgcgctcACATTGGACACGCTTCGGTGGTTGGACACAGGCCGTCGTAGGGGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTacac                                                                                                         <  2:4768846/148‑1 (MQ=17)

                                                       ctcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTacaca                                                                                                        <  1:4928978/151‑1 (MQ=255)

                                                        tcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTacacac                                                                                                       <  2:143069/151‑1 (MQ=255)

                                                          gctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGCTCACCGCTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGc                                                                                                     >  2:2628646/1‑151 (MQ=255)

                                                             cacaTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATc                                                                                                  <  1:3351914/151‑1 (MQ=255)

                                                             cacaTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATc                                                                                                  <  1:3435406/151‑1 (MQ=255)

                                                              acaTTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCg                                                                                                 >  1:970057/1‑151 (MQ=255)

                                                                  tGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCggggg                                                                                             >  1:1822023/1‑151 (MQ=255)

                                                                    gACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGtt                                                                                           >  1:4991915/1‑151 (MQ=255)

                                                                     acacGCAGCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTc                                                                                          <  2:4075433/151‑1 (MQ=255)

                                                                      cacGCATCGGTGGTTGGACACAGGCCGTCGCAGGAGCGGGGTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGCTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCgcggatccc                                                                                         >  2:1719453/1‑146 (MQ=11)

                                                                         gCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATC‑GGGGTTCGGcac                                                                                     <  2:790722/151‑1 (MQ=255)

                                                                           agcGGTGGTTGGACACAGGCCGTCGTAGGAGGGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTACCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGcaca                                                                                    <  1:2421637/149‑1 (MQ=255)

                                                                           aTCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCAGGGGTTCGGcacc                                                                                    >  1:1020927/1‑150 (MQ=255)

                                                                                tggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCcg                                                                               <  2:4486622/151‑1 (MQ=255)

                                                                                  gtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCcgtc                                                                             >  1:2966622/1‑151 (MQ=255)

                                                                                  gtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCcgtc                                                                             <  2:1668610/151‑1 (MQ=255)

                                                                                    tGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCcgtcgt                                                                           >  2:756257/1‑151 (MQ=255)

                                                                                      gACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAg                                                                         <  1:842568/151‑1 (MQ=255)

                                                                                       acacAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCCACACACGCATCGGGGGTTCGGCACACGCCGTCGTacg                                                                        >  2:373026/1‑149 (MQ=255)

                                                                                        cacaGGCCGTCGTGGGAGCGGGTTCCCCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGa                                                                       <  2:899381/151‑1 (MQ=255)

                                                                                          caGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGc                                                                     <  1:4305372/151‑1 (MQ=255)

                                                                                           aGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCg                                                                    <  1:3180295/151‑1 (MQ=255)

                                                                                            ggCCGTCGTAGTAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCgg                                                                   <  2:3680404/151‑1 (MQ=255)

                                                                                              ccGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCgggg                                                                 >  1:1341585/1‑150 (MQ=255)

                                                                                                gtcgtAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCTTAGTCGCGTGTAAACCCGCCCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCCTAGGAGCgggcgg                                                               >  2:125098/1‑148 (MQ=17)

                                                                                                gtcgtAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTc                                                               <  1:2893881/151‑1 (MQ=255)

                                                                                                    tgggAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCCCCGTATCCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCAccc                                                           <  1:3537908/149‑1 (MQ=255)

                                                                                                    tAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCAccc                                                           <  1:5199520/151‑1 (MQ=255)

                                                                                                    tAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACACGCCGTCGTAGGCGCGCGTTCAccc                                                           >  2:3567979/1‑151 (MQ=17)

                                                                                                     aGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATc                                                                                                  <  2:3077108/111‑1 (MQ=255)

                                                                                                       gcgCGGGTGCGCCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCcgcg                                                        <  1:4969917/149‑1 (MQ=255)

                                                                                                       gAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCcgcg                                                        >  1:1431678/1‑151 (MQ=255)

                                                                                                        aGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGa                                                       >  1:2696833/1‑151 (MQ=255)

                                                                                                            ggTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAgg                                                   <  2:3592331/151‑1 (MQ=255)

                                                                                                            ggTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTCCGGCACAGGCCGTCGTAGGCGCGGGCTCACCCGCGAAAgg                                                   >  2:4323754/1‑151 (MQ=17)

                                                                                                             gTTCACCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGCCACAGGCCGTCGTAGGAGCGGGCGCACCCGCGAAAggg                                                  >  2:314474/1‑151 (MQ=17)

                                                                                                              ttCGCCCGCGCCTGGGCCGGTCCCGCTAGCCAATCTCCTCCCGCCGTTCTCCCTTGCCGCCGCCCCCGTATTCGCGGGTAACCCCCCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGggc                                                 <  2:2263485/151‑1 (MQ=17)

                                                                                                                 aCCCGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGCTCACCCGCCAAACggccgg                                              >  2:1199227/1‑151 (MQ=17)

                                                                                                                   ccGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCTCTCCTACACACGCATCGGGTGGTCGGCGCAGGCCATCGTAGGAGCGGGTGCGCCCGCGAAAGggccgtcc                                            >  1:246613/1‑151 (MQ=14)

                                                                                                                   ccGCGAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTAGTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGggccggcc                                            <  2:2506320/151‑1 (MQ=255)

                                                                                                                    cgcgAGTGGGCCGGTCCTGCTAGCCAACCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATCCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGccc                                           <  2:2158712/151‑1 (MQ=255)

                                                                                                                    cgcgAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACCCGCATCGGGCGTTCGGCCCAGGCCGTCGTAGGAGCGCGGTCACCCGCGCAAGGGACGGccc                                           >  2:952614/1‑151 (MQ=17)

                                                                                                                     gcgAATGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGCGCGGGTGCACCCGCGAAAGGGCCGGCCct                                          >  1:1056360/1‑151 (MQ=255)

                                                                                                                          tGGGCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGTTTCGGCCCAGGCCGTCGTATGAGCGGGCTCACCCGCGCACGTGCCGCCCctgctag                                     >  2:2923234/1‑149 (MQ=17)

                                                                                                                             gCCGGTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCa                                  >  1:4960643/1‑151 (MQ=255)

                                                                                                                                ggTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAAtc                               <  1:5171191/151‑1 (MQ=255)

                                                                                                                                 ggccggctgGCCACTGTCCCCCCGCCGTTCACCGTTGCCGGCACCGCCGTACTCGCGGGTAACCCCGCTCCTACACACAAAT‑‑‑‑‑‑‑‑‑‑GCAAAGGCCGGCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCTTGCTGGCCAATCTCCACccgccg                    <  2:211202/146‑1 (MQ=2)

                                                                                                                                 gTCCTGCTAGCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACCCGCATCGGGGGCTCGGCCCACGCCGTCGTAGCACCGGCCTCACCCGCCAAACCCCCGGCCCCCCTGCCCacccc                              >  2:2585683/1‑147 (MQ=14)

                                                                                                                                   ggtgctaGCCGATCTCCTCCCGCCGTTCACCGGTGCCGCCGCCAGCGTATTCGCGGGTAAGCCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTcc                            <  2:5109937/149‑1 (MQ=17)

                                                                                                                                      gctagCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCAcc                         <  1:5370825/147‑1 (MQ=255)

                                                                                                                                       ctagCCAATCTCTTCCCGCCGCTCCCCGTTGCCCCCGCCACCGTATCCGCGCGTACTCCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCAccc                        <  2:1822023/148‑1 (MQ=17)

                                                                                                                                        tagCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACccg                       <  1:5417131/149‑1 (MQ=255)

                                                                                                                                        tagCCAATCTCCTCCCGACGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACccg                       <  1:1789504/149‑1 (MQ=255)

                                                                                                                                          gCCAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGGAGGAGCGGGTTCACCCGCGAAAGCGCCGCCCCTGCTGGCCAATCTCCACccgcc                     >  1:3070991/1‑151 (MQ=255)

                                                                                                                                          gCCAATCTCCACCCGCCGTTCACCGTTGCCGGCACCACCGTATTCGCGGGTAAACCCGCTCCTACACACAAAT‑‑‑‑‑‑‑‑‑‑GCACAGGCCTTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCTTGCTGGCCAATCTCCACCCGCCGTTCACCGtt           <  1:5224628/151‑1 (MQ=0)

                                                                                                                                           ccAATCTCCGCGCGCCGTTCACCGTTGCCGGCACCACCGTATTCGCGGGTAAACCCGCTCCTACACACAAAT‑‑‑‑‑‑‑‑‑‑GCACAGGCCTTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCTTGCTGGCCAATCTCCACCCGCCGTTCACCGTTg          <  1:2821315/151‑1 (MQ=2)

                                                                                                                                            cAATCTCCTCCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGa                   >  1:3982761/1‑151 (MQ=255)

                                                                                                                                               cctcctcCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGATCa                <  1:1698769/150‑1 (MQ=255)

                                                                                                                                               cctcctcCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGATCa                <  1:4625437/150‑1 (MQ=255)

                                                                                                                                                ctcctcCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAACCCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGATCAc               <  1:1488973/151‑1 (MQ=255)

                                                                                                                                                ctcCACCCGCCGTTCACAGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCGCCTACACACAAAT‑‑‑‑‑‑‑‑‑‑GCACAGGCCTTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCTTGCTGGCCAATCTCCACCCGCCGTTCACCGTTgccgcc     <  1:212382/151‑1 (MQ=2)

CCGCGGTGGCCGTCACAGATACGCGGCCTGAGAGATCAAGCACATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGTCCTGCTGGCCAATCTCCACCCGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACGCATCGGGGGTTCGGCACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCGATCACCGTTGCCGCCGCC  >  CP009974/25591‑25898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`