BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,157,352	C→T	intergenic (+178/+65)	RPPX_22875 → / ← dnaE2	integrase/DNA polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,157,352	0	C	T	99.0%	702.3 / NA	195	intergenic (+178/+65)	RPPX_22875/dnaE2	integrase/DNA polymerase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (104/89); minor base G (0/2); total (104/91)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GGGGGGCTCAGGCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAACGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAGGTGCCGC  >  CP009974/5157204‑5157498

       ccAGGCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGgct                                                                                                                                           <  1:760582/150‑1 (MQ=255)

        cAGGCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGgctg                                                                                                                                          >  1:673880/1‑151 (MQ=255)

         aGGCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGgctgc                                                                                                                                         >  2:1775926/1‑151 (MQ=255)

          ggCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGgctgct                                                                                                                                        <  2:2107888/151‑1 (MQ=255)

          ggCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGCTGacggct                                                                                                                                        >  2:2063844/1‑151 (MQ=255)

           ggcTAGTGGGAAACGTGGCTGTCGACCAAGGTATAACCCACCGTGGCCGATGAATGGTTGGGGCAGGCTCACGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCtt                                                                                                                                       <  2:766075/149‑1 (MQ=255)

             cTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTcg                                                                                                                                     <  1:4450028/151‑1 (MQ=255)

              tAGTGGCAAACGTGGCGATCGACCAGGGTATAACGCACCGCGGCGGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGGGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTcgc                                                                                                                                    <  2:2789633/151‑1 (MQ=37)

               aGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTcgcg                                                                                                                                   >  2:1558761/1‑151 (MQ=255)

               aGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTcgcg                                                                                                                                   >  2:1725335/1‑151 (MQ=255)

                  ggCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGc                                                                                                                                >  1:624329/1‑151 (MQ=255)

                   gCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCt                                                                                                                               >  1:49793/1‑151 (MQ=255)

                    gaaaGGTGGCTGTCGACCAAGGTGTAACGGGCCGTGGCCGATGAATGGTTGGGGCGGGCTCAGGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAGCACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTc                                                                                                                              <  2:3898041/150‑1 (MQ=25)

                    cAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTc                                                                                                                              <  1:1418821/151‑1 (MQ=255)

                      aaggTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGc                                                                                                                            <  2:3107670/148‑1 (MQ=255)

                      aaCGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGc                                                                                                                            >  2:3793923/1‑151 (MQ=255)

                       aCGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGcc                                                                                                                           <  1:1051905/151‑1 (MQ=255)

                        cGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGccc                                                                                                                          <  1:540393/151‑1 (MQ=255)

                         gTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCg                                                                                                                         <  1:399289/151‑1 (MQ=255)

                           gggtGTCGACCAAGGTATAGCGCGCCGTGGCCGATGAATGGTCGGGGCAGGCTCAAGGGTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCACGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCt                                                                                                                       <  2:2707495/148‑1 (MQ=21)

                              tGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTccc                                                                                                                    >  2:4993697/1‑151 (MQ=255)

                              tGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTccc                                                                                                                    <  2:54167/151‑1 (MQ=255)

                              tGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTccc                                                                                                                    >  1:4715023/1‑151 (MQ=255)

                                tCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCcac                                                                                                                  <  1:4676995/151‑1 (MQ=255)

                                  gACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCGCGCCCGCCCCCACAg                                                                                                                >  2:4870935/1‑151 (MQ=255)

                                      aaGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGaa                                                                                                            <  2:65558/151‑1 (MQ=255)

                                        ggTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAAcc                                                                                                          <  2:833627/151‑1 (MQ=255)

                                        ggTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGGGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAAcc                                                                                                          >  1:2509355/1‑151 (MQ=255)

                                          tataACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACACGGAACcgc                                                                                                        >  2:1583151/1‑151 (MQ=255)

                                          tagaaCGCACCGTGGCCTATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTGACGGTGGGAGCGGGCATGCCCGCGACCCCCGGCGAGGCCGGTGCCCTGCAATGCGGTCGCTGCTTCGCGGGCTCGCCCGCTACCACAGGGAACcgc                                                                                                        <  2:2556951/148‑1 (MQ=21)

                                           ataACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCACGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACcgcg                                                                                                       >  1:3910010/1‑151 (MQ=255)

                                           ataACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACcgcg                                                                                                       >  2:3881310/1‑151 (MQ=255)

                                           ataACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCGGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACcgcg                                                                                                       <  2:4768040/151‑1 (MQ=255)

                                               cGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCTCGCTCCCTCAGGGAACCGCGCAAg                                                                                                   >  2:416358/1‑151 (MQ=255)

                                               cGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAg                                                                                                   >  1:4474386/1‑151 (MQ=255)

                                               cGCAACGTGGCCTATGGATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAg                                                                                                   <  2:5338082/151‑1 (MQ=255)

                                                 cACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGtt                                                                                                 <  2:3526797/151‑1 (MQ=255)

                                                  aCCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCCAGTTc                                                                                                >  1:3665346/1‑151 (MQ=255)

                                                  aCCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTc                                                                                                >  1:3685935/1‑151 (MQ=255)

                                                     gTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTc                                                                                                                                      >  1:101632/1‑110 (MQ=255)

                                                      tGGCCGATGAATGGTTGGGGCAGGCTCAGGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAg                                                                                            <  1:1080572/151‑1 (MQ=255)

                                                        gCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGGCCGCTCCCACAGGGAACCGCGCAAGATACGAGCg                                                                                          >  2:1637856/1‑151 (MQ=255)

                                                           gATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTc                                                                                                                              >  1:2125587/1‑112 (MQ=255)

                                                               aaTGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTgggg                                                                                    >  1:3889155/1‑150 (MQ=255)

                                                                gtggtggGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTc                                                                                  <  2:583210/150‑1 (MQ=255)

                                                                 tGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCa                                                                                 <  1:4384437/151‑1 (MQ=255)

                                                                 tGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCa                                                                                 <  1:1058123/151‑1 (MQ=255)

                                                                 tGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCa                                                                                 >  1:3222065/1‑151 (MQ=255)

                                                                   gTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGt                                                                               >  1:770466/1‑151 (MQ=255)

                                                                      ggggCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGa                                                                            <  2:2925530/151‑1 (MQ=255)

                                                                       gggCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGaa                                                                           >  1:5148671/1‑151 (MQ=255)

                                                                        ggCATGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGaaa                                                                          >  1:4165126/1‑151 (MQ=255)

                                                                         gCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAAt                                                                         <  2:2303515/151‑1 (MQ=255)

                                                                         gCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGGCAGTGGAAAt                                                                         >  1:89226/1‑151 (MQ=255)

                                                                           aGGCTCAAGGCGGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATcc                                                                       <  1:5260993/151‑1 (MQ=255)

                                                                            ggCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATccc                                                                      >  2:4226397/1‑151 (MQ=255)

                                                                                cacggCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCt                                                                  <  1:463613/148‑1 (MQ=255)

                                                                                cAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCt                                                                  >  1:5275200/1‑151 (MQ=255)

                                                                                cAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGCGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGcc                                                                  >  2:3933746/1‑150 (MQ=255)

                                                                                  aGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGGGGCTGCTGCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGCTCCTGGCGTTCGGGTGAGTGGAAATCACGGCCGc                                                                >  2:1930546/1‑151 (MQ=18)

                                                                                   ggCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCg                                                               >  2:2497477/1‑151 (MQ=255)

                                                                                    gCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGACCACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGt                                                              <  2:4234463/151‑1 (MQ=255)

                                                                                    gCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGt                                                              <  1:3486178/151‑1 (MQ=255)

                                                                                       gCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACa                                                           <  1:4325059/151‑1 (MQ=255)

                                                                                        gaGGCGGTCAATGGGGGAGCGGGCATGCCCGCGAACAGCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACAt                                                          <  1:3289512/150‑1 (MQ=255)

                                                                                        cAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACAt                                                          >  1:4175772/1‑151 (MQ=255)

                                                                                        cAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACAt                                                          >  2:4087365/1‑151 (MQ=255)

                                                                                         aGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATc                                                         >  2:3822943/1‑151 (MQ=255)

                                                                                         aGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATc                                                         >  1:1159218/1‑151 (MQ=255)

                                                                                         aGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATc                                                         >  2:398522/1‑151 (MQ=255)

                                                                                          ggCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATcc                                                        <  1:1553465/151‑1 (MQ=255)

                                                                                               tcggtgggggAGCGGGCATGCCGGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGccc                                                   <  2:5222210/144‑1 (MQ=255)

                                                                                                 aaTGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGGCAGTGGAAATCCCGGCTGCGTACATCCAGACCtt                                                 >  2:3758466/1‑151 (MQ=255)

                                                                                                   tgtgGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCCTgc                                               >  1:4549807/1‑151 (MQ=255)

                                                                                                   tgtgGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGCCTGCGTCCATCCCGCCCttcc                                               >  2:2831501/1‑149 (MQ=255)

                                                                                                    gtgGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTgca                                              <  2:4978904/151‑1 (MQ=255)

                                                                                                          gCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCg                                        >  2:2292258/1‑151 (MQ=255)

                                                                                                               cATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTc                                   <  1:2645306/151‑1 (MQ=255)

                                                                                                                aTGCCCGGGAAGACGGGCAAGGCCGGTGCGCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTca                                  <  2:2717979/151‑1 (MQ=255)

                                                                                                                 tGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTcac                                 <  1:3663930/151‑1 (MQ=255)

                                                                                                                  gCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTcaca                                <  1:1861499/151‑1 (MQ=255)

                                                                                                                   cccGCGAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACAt                               <  2:62761/151‑1 (MQ=255)

                                                                                                                        gAACACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACAtcctcc                          <  1:4616902/151‑1 (MQ=255)

                                                                                                                         aaCACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCa                         >  2:10323/1‑151 (MQ=255)

                                                                                                                         aaCACCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCa                         <  2:3851757/151‑1 (MQ=255)

                                                                                                                           cacCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAAc                       <  2:507858/151‑1 (MQ=255)

                                                                                                                            acCGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAAcc                      <  2:4175772/151‑1 (MQ=255)

                                                                                                                              cGGCAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAAccgc                    >  1:2348875/1‑151 (MQ=255)

                                                                                                                               ggCAAGGCCGGTGCCCTGCAAGGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAAccgcc                   <  2:5026983/151‑1 (MQ=255)

                                                                                                                                 cAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACcgccgc                 <  2:1010854/151‑1 (MQ=255)

                                                                                                                                 cAAGGCCGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACcgccgc                 >  1:3947537/1‑151 (MQ=255)

                                                                                                                                      gcGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAAt            <  2:2821376/150‑1 (MQ=255)

                                                                                                                                       cGGTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATc           >  2:1071727/1‑151 (MQ=255)

                                                                                                                                         gTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAg         >  1:1285876/1‑151 (MQ=255)

                                                                                                                                         gTGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAg         <  1:1725335/151‑1 (MQ=255)

                                                                                                                                          tGCCCTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAgg        >  1:5026312/1‑151 (MQ=255)

                                                                                                                                            cccTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAGGTg      <  2:4581203/151‑1 (MQ=255)

                                                                                                                                            cccTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAGGTg      >  1:2120023/1‑151 (MQ=255)

                                                                                                                                             ccTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAGGtcc     >  2:4497732/1‑149 (MQ=255)

                                                                                                                                              cTGCAATGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAACTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAGGTGcc    <  1:3183826/151‑1 (MQ=255)

GGGGGGCTCAGGCCTAGTGGCAAACGTGGCTGTCGACCAAGGTATAACGCACCGTGGCCGATGAATGGTTGGGGCAGGCTCAAGGCTGCAGGCGGTCAATGTGGGAGCGGGCATGCCCGCGAACACCGGCAAGGCCGGTGCCCTGCAACGCGGTGGCTGCTTCGCGGGCTCGCCCGCTCCCACAGGGAACCGCGCAAGTTCCGAGCGTTGGGGTCAGTGGAAATCCCGGCTGCGTACATCCAGCCCTTGCAGCAGCGGACTCACATCCTCCAACCGCCGCGCAATCAGGTGCCGC  >  CP009974/5157204‑5157498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`