BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,513,872	T→C	Q264R (CAG→CGG)	RPPX_24600 ←	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,513,872	0	T	C	99.4%	572.0 / NA	178	Q264R (CAG→CGG)	RPPX_24600	integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (79/97); minor base G (0/1); total (79/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

GTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTGCAGCCTGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACTCAGA  >  CP009974/5513724‑5514008

gTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGa                                                                                                                                        >  2:3899777/1‑151 (MQ=255)

   cGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGCTGGACAGTTCTAAGCTACAGCCCGAGcc                                                                                                                                     >  2:4245128/1‑151 (MQ=255)

     agaCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCt                                                                                                                                   <  1:3529005/151‑1 (MQ=255)

     agaCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCt                                                                                                                                   <  1:3726856/151‑1 (MQ=255)

      gaCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTg                                                                                                                                  <  2:2145909/151‑1 (MQ=255)

       aCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAGTTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGt                                                                                                                                 <  2:5090597/151‑1 (MQ=255)

       aCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGt                                                                                                                                 >  1:5332714/1‑151 (MQ=255)

       aCTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGt                                                                                                                                 >  2:2567546/1‑151 (MQ=255)

         ttgtGGCTCGGGCAGGAGTGGCGGGCGGGTATCAAGACGATGGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTAt                                                                                                                               <  2:4429469/148‑1 (MQ=37)

         ttATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTAt                                                                                                                               <  1:1131848/151‑1 (MQ=255)

         ttATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTAt                                                                                                                               >  1:1860532/1‑151 (MQ=255)

          tATGGCTGAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAGCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTAtt                                                                                                                              <  2:4592493/151‑1 (MQ=255)

          tATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTAtt                                                                                                                              <  1:2423656/151‑1 (MQ=255)

                tCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAg                                                                                                                        >  2:1520548/1‑151 (MQ=255)

                  aggcaggAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAggg                                                                                                                      >  2:425891/1‑151 (MQ=255)

                  aggcaggAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAggg                                                                                                                      <  2:4704230/151‑1 (MQ=255)

                      aggAGTGGCAGGGGGGTATCAATACGATTGAAGGAACCGGGGCCGCCTTGCGGCCCTGCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCAAGCCCTGAATTCCACAGGGCTCa                                                                                                                  <  2:3036610/151‑1 (MQ=25)

                      aggAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCa                                                                                                                  <  1:813050/151‑1 (MQ=255)

                      aggAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCa                                                                                                                  >  2:1737856/1‑151 (MQ=255)

                        gAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAgg                                                                                                                >  1:4831041/1‑151 (MQ=255)

                           tGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCa                                                                                                             <  1:2046835/151‑1 (MQ=255)

                            ggCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCAt                                                                                                            >  1:5124876/1‑151 (MQ=255)

                               gggCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGa                                                                                                         <  1:1431036/150‑1 (MQ=255)

                                ggCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAg                                                                                                        >  2:4912795/1‑151 (MQ=255)

                                 gCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGt                                                                                                       >  2:1418240/1‑151 (MQ=255)

                                   gggTATCAATACGATTGAATGAACCGGGGCCGCCTTGCTCCCCTTCGCGGAATCGCCCTCGCTCCCGCACTTTGCACCGCCGCCCACAACTTCCACAGTTCTACGACACCTCCCGAGCCCTCTAATCCACTGGGCCCACGCCATCCAGtcc                                                                                                     >  2:3249275/1‑150 (MQ=255)

                                     gTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCa                                                                                                   >  1:2670475/1‑151 (MQ=255)

                                     gTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCa                                                                                                   <  2:545228/151‑1 (MQ=255)

                                           aTACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGa                                                                                             >  1:3620315/1‑151 (MQ=255)

                                           aTACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGa                                                                                             <  1:1126455/151‑1 (MQ=255)

                                            tACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGAt                                                                                            >  1:1552180/1‑151 (MQ=255)

                                             aCGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATg                                                                                           >  1:4405819/1‑151 (MQ=255)

                                             aCGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATg                                                                                           >  1:4907860/1‑151 (MQ=255)

                                               gATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCg                                                                                         <  1:1682879/151‑1 (MQ=255)

                                               gATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCg                                                                                         >  2:1154600/1‑151 (MQ=255)

                                                    aatgaaCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCAt                                                                                    <  1:1234849/151‑1 (MQ=255)

                                                    aatgaaCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCCCAGGCCATTGAGTCTCAGCTTGATGCGATCAt                                                                                    >  1:1383100/1‑151 (MQ=255)

                                                         aCCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTg                                                                               >  1:902649/1‑151 (MQ=255)

                                                             gggCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTgtagt                                                                           <  2:2376002/151‑1 (MQ=255)

                                                              ggCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCGCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTgtagta                                                                          <  2:3574448/151‑1 (MQ=255)

                                                                  gccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGa                                                                      >  1:5333362/1‑151 (MQ=255)

                                                                  gccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGa                                                                      <  1:4493847/151‑1 (MQ=255)

                                                                   ccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACCGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGaa                                                                     <  2:1331165/151‑1 (MQ=255)

                                                                   ccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGaa                                                                     >  1:3986673/1‑151 (MQ=255)

                                                                   ccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGaa                                                                     <  1:2245132/151‑1 (MQ=255)

                                                                   ccTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTAGCAGCTTGATGCGATCATGGTTGTAGTAGTGaa                                                                     >  1:4671607/1‑151 (MQ=255)

                                                                    cTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAAt                                                                    >  2:1010898/1‑151 (MQ=255)

                                                                      tGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGt                                                                  <  1:3334249/151‑1 (MQ=255)

                                                                      tGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGt                                                                  <  1:190771/151‑1 (MQ=255)

                                                                        cGGCCGTTCGCGGGCTCGCCCTCGGTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTAc                                                                <  2:5009721/151‑1 (MQ=255)

                                                                        cGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTAc                                                                <  1:2058715/151‑1 (MQ=255)

                                                                        cGGCCCTTCGCGGGCTCGCCCTCACTCCAACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTCAGCTACAGCCCGAGCCCTGCATTACAGAAGGCGCAGGCCATTGAGGCTCAGCATCATGCGATCATGGTTGTAGGAGTGAATGTAc                                                                >  2:3803918/1‑151 (MQ=17)

                                                                         ggCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACt                                                               >  1:3418139/1‑151 (MQ=255)

                                                                          gCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGTCAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTc                                                              <  1:3193404/151‑1 (MQ=255)

                                                                          gCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGGTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTc                                                              <  2:5168596/151‑1 (MQ=255)

                                                                           cccTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGa                                                                                        >  2:904886/1‑124 (MQ=255)

                                                                            ccTTCGGGGGCACGCTCTCGAGCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCAt                                                            <  2:2985160/151‑1 (MQ=14)

                                                                              ttCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATcc                                                          <  1:1068871/151‑1 (MQ=255)

                                                                               tCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCa                                                         <  1:4732573/151‑1 (MQ=255)

                                                                                cgcgGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAg                                                        >  2:1050087/1‑151 (MQ=255)

                                                                                 ccaggCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGa                                                       <  1:301487/148‑1 (MQ=255)

                                                                                  cgGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAc                                                      <  1:1307773/151‑1 (MQ=255)

                                                                                    ggCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAccc                                                    >  1:3858881/1‑151 (MQ=255)

                                                                                     gCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAccc                                                    <  1:2182593/150‑1 (MQ=255)

                                                                                     gCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAccc                                                    >  2:2182593/1‑150 (MQ=255)

                                                                                     gCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCg                                                   <  1:662717/151‑1 (MQ=255)

                                                                                       tCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGct                                                 <  2:3986673/151‑1 (MQ=255)

                                                                                        cGCCCTCGCTCCCACAATTTGGCCTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGctc                                                <  1:537437/151‑1 (MQ=255)

                                                                                         gCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGctct                                               <  2:3481355/151‑1 (MQ=255)

                                                                                           ccTCGCTCCCATAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCa                                             <  1:4968509/151‑1 (MQ=255)

                                                                                           ccTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCa                                             <  1:529797/151‑1 (MQ=255)

                                                                                           ccTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCa                                             <  1:1192755/151‑1 (MQ=255)

                                                                                             tCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGt                                                                  >  1:1219889/1‑128 (MQ=255)

                                                                                             tCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGt                                                                  <  2:1219977/128‑1 (MQ=255)

                                                                                               gCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtc                                         >  2:3362805/1‑151 (MQ=255)

                                                                                               gCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtc                                         >  2:3362726/1‑151 (MQ=255)

                                                                                               ccTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGTCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCTGCtc                                         <  1:2749432/150‑1 (MQ=255)

                                                                                                cTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtca                                        >  1:4544400/1‑151 (MQ=255)

                                                                                                cTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtca                                        >  1:2009331/1‑151 (MQ=255)

                                                                                                   ccACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCtcatca                                     >  1:5331419/1‑151 (MQ=255)

                                                                                                        aTTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGAccc                                                    >  1:663441/1‑131 (MQ=255)

                                                                                                              aCTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGaa                          >  1:3750508/1‑151 (MQ=12)

                                                                                                               cTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGaaa                         <  2:2294032/151‑1 (MQ=12)

                                                                                                               cTGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGaaa                         <  2:2289215/151‑1 (MQ=17)

                                                                                                                tGCCCCCCAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAAc                        <  2:4116542/151‑1 (MQ=9)

                                                                                                                       cAAAAGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAg                 <  1:425891/151‑1 (MQ=17)

                                                                                                                        aaaaGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAgg                >  2:511505/1‑151 (MQ=17)

                                                                                                                        aaaaGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAgg                >  2:4486554/1‑151 (MQ=12)

                                                                                                                          aaGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTa              <  2:2086299/151‑1 (MQ=14)

                                                                                                                          aaGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTa              >  1:2915044/1‑151 (MQ=12)

                                                                                                                           aGTTGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAg             >  2:3145214/1‑151 (MQ=12)

                                                                                                                             ttGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaa           <  1:1060976/151‑1 (MQ=12)

                                                                                                                             ttGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaa           <  2:2915044/151‑1 (MQ=12)

                                                                                                                              tGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaaa          <  1:1430395/151‑1 (MQ=17)

                                                                                                                              tGGACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaaa          <  2:2244543/151‑1 (MQ=17)

                                                                                                                               ggAAAGTACTAAGCTACAGCCAGATCCCTGTGGTGCACAGGGCTCAGGCCATGGAGTCCCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTAATCCAGTCCCGCTCTCAGCTCATCAATGCTCTCGAAACGGCTCAGGTAGaaaa         <  2:4392461/151‑1 (MQ=11)

                                                                                                                                gACAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGaaaaa        <  2:2781722/151‑1 (MQ=14)

                                                                                                                                  cAGTTCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACt      <  2:1450865/151‑1 (MQ=14)

                                                                                                                                     ttCTAAGCTACAGCCCGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACTCAg   >  1:2843722/1‑151 (MQ=9)

                                                                                                                                      tCTAAGCTCCAGCCGGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACTCAGa  <  2:4052886/151‑1 (MQ=14)

GTTCGAGACTTATGGCTCAGGCAGGAGTGGCAGGCGGGTATCAATACGATTGAATGAACCGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCTCGCTCCCACAATTTGGACTGCCCCCCAAAAGTTGGACAGTTCTAAGCTGCAGCCTGAGCCCTGTATTCCACAGGGCTCAGGCCATTGAGTCTCAGCTTGATGCGATCATGGTTGTAGTAGTGAATGTACTCATCCAGACCCGCTCTCAGCTCATCAATGCTCTCGAAACGCTTCAGGTAGAAAAACTCAGA  >  CP009974/5513724‑5514008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`