BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	5,597,121	C→T	T614T (ACC→ACT)	RPPX_24915 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	5,597,121	0	C	T	99.7%	1019.1 / NA	357	T614T (ACC→ACT)	RPPX_24915	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (167/189); minor base G (0/1); total (167/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

CTTTAGCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACCACTGCGCGTGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCACCGGAATACACCCTG  >  CP009974/5597013‑5597268

cTTTAGCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTcc                                                                                                           <  1:1049791/151‑1 (MQ=2)

 tttAGCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCcg                                                                                                          <  1:3081263/151‑1 (MQ=9)

   tAGCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCcggc                                                                                                        <  2:3271113/151‑1 (MQ=9)

    aGCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCcggcg                                                                                                       <  1:1199400/151‑1 (MQ=9)

     gCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCcggcgg                                                                                                      >  1:2328440/1‑151 (MQ=14)

         ggaggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCcggcgg                                                                                                      >  1:1829913/1‑147 (MQ=12)

         ggaggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggt                                                                                                  >  1:2011799/1‑151 (MQ=9)

          gaggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtg                                                                                                 >  2:1121694/1‑151 (MQ=12)

          gaggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtg                                                                                                 >  2:1868527/1‑151 (MQ=9)

          gaggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtg                                                                                                 >  2:824739/1‑151 (MQ=12)

          gaggGGCAGCTGGATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtg                                                                                                 <  2:4928761/151‑1 (MQ=255)

           gggTGCAGCGGTAGTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtgg                                                                                                <  1:5398723/150‑1 (MQ=12)

           aggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtgg                                                                                                <  1:5364898/151‑1 (MQ=12)

            ggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtggt                                                                                               <  1:214977/151‑1 (MQ=12)

            ggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtggt                                                                                               <  2:2195695/151‑1 (MQ=14)

            ggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtggt                                                                                               >  1:1299209/1‑151 (MQ=12)

            ggTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtggt                                                                                               >  2:2825690/1‑151 (MQ=12)

             gTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtggtg                                                                                              >  2:4421547/1‑151 (MQ=12)

              tGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCggtggtggtgg                                                                                             >  2:2112256/1‑151 (MQ=12)

                 aGCGGGATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGttt                                                                                          <  2:2138367/151‑1 (MQ=12)

                  gCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTa                                                                                         <  1:4866992/151‑1 (MQ=12)

                  gCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTa                                                                                         >  2:2579679/1‑151 (MQ=9)

                  gCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCAAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTa                                                                                         <  1:3441510/151‑1 (MQ=255)

                   gggTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTaa                                                                                        <  1:4329354/150‑1 (MQ=12)

                    ggTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTaaa                                                                                       >  2:2467512/1‑151 (MQ=12)

                    ggTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTaaa                                                                                       <  2:653502/151‑1 (MQ=12)

                        tttGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCa                                                                                   <  2:1939145/151‑1 (MQ=16)

                         ttGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAg                                                                                  <  1:764828/151‑1 (MQ=16)

                           gAAGAGCGGCATGGCACAGGTGGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAggg                                                                                <  2:3673441/151‑1 (MQ=255)

                            aaGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGa                                                                               >  2:504231/1‑151 (MQ=255)

                            aaGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGa                                                                               <  1:4543328/151‑1 (MQ=19)

                            aaGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGa                                                                               >  2:1372747/1‑151 (MQ=25)

                              gagCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGAtg                                                                             <  1:1920853/151‑1 (MQ=255)

                                 cgggttGGAACAAGTAGAAGAGATGAAAGCGTTTCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGAGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGAtgctg                                                                          <  1:1008125/146‑1 (MQ=11)

                                  ggCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGAtgttgt                                                                         <  1:36220/151‑1 (MQ=255)

                                   gCATGGCACAGGTTGAAGAGATGAAAGCGGTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTtgtg                                                                        <  2:3472125/151‑1 (MQ=255)

                                      tGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGtt                                                                     <  2:158830/151‑1 (MQ=255)

                                      gggCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGtt                                                                     <  2:3135935/150‑1 (MQ=255)

                                        gCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCa                                                                   >  1:5115325/1‑151 (MQ=255)

                                         cacaGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCaa                                                                  >  1:2479856/1‑151 (MQ=255)

                                          acaGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAAc                                                                 <  1:5194/151‑1 (MQ=255)

                                             ggTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATc                                                              >  2:1168627/1‑151 (MQ=255)

                                               ttGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCtt                                                            >  1:4607597/1‑151 (MQ=255)

                                                tGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTa                                                           <  2:5348180/151‑1 (MQ=255)

                                                 gAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTAt                                                          <  2:1982888/151‑1 (MQ=255)

                                                 gAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTAt                                                          <  1:2612618/151‑1 (MQ=255)

                                                  aaGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATc                                                         >  2:1109369/1‑151 (MQ=255)

                                                  aaGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGCGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATc                                                         <  1:4093196/151‑1 (MQ=255)

                                                   agagATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCg                                                        <  2:3748741/151‑1 (MQ=255)

                                                     agaTGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCt                                                      >  2:471442/1‑151 (MQ=255)

                                                     agaTGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCt                                                      >  1:2084428/1‑151 (MQ=255)

                                                       aTGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCa                                                    <  1:70532/151‑1 (MQ=255)

                                                          aaaGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGa                                                 <  2:4771008/151‑1 (MQ=255)

                                                           aaGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAacac                                                <  1:3420448/151‑1 (MQ=255)

                                                             gCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACgg                                              >  2:406289/1‑151 (MQ=255)

                                                               gTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTgg                                                                       >  1:999949/1‑124 (MQ=255)

                                                                 tGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTa                                          <  1:125294/151‑1 (MQ=11)

                                                                 tGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTa                                          <  2:4962864/151‑1 (MQ=255)

                                                                  gcgcATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTac                                         <  1:874581/151‑1 (MQ=255)

                                                                     cATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTacaac                                      <  2:949044/151‑1 (MQ=14)

                                                                     cATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTacaac                                      >  1:2988237/1‑151 (MQ=14)

                                                                      aTCTCGGGTGGCCGCACGGTGTCTATCGAGCTGACCAGTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGGTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGGGGTTCAACATCTTATCGCTCAACACGGCCTacaaca                                     <  2:4252177/151‑1 (MQ=11)

                                                                      aTCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTacaaca                                     >  2:1309472/1‑151 (MQ=255)

                                                                      aTCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTacaaca                                     <  1:1709192/151‑1 (MQ=14)

                                                                       tctcGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAg                                    >  1:2793457/1‑151 (MQ=255)

                                                                        ctcGGGTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGc                                   >  1:4382072/1‑151 (MQ=255)

                                                                           gggTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACTCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTg                                <  2:5092393/151‑1 (MQ=255)

                                                                           gggTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTg                                <  2:3952275/151‑1 (MQ=255)

                                                                             gTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCa                              >  1:3212279/1‑151 (MQ=255)

                                                                             gTAGCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCa                              <  2:1267718/151‑1 (MQ=14)

                                                                                gCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCagaa                           >  1:3137048/1‑151 (MQ=255)

                                                                                gCCGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCagaa                           >  2:1266504/1‑151 (MQ=255)

                                                                                 ccGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTCCAACAGCCTGCagaag                          >  2:1785918/1‑151 (MQ=255)

                                                                                 ccGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCagaag                          <  2:2489500/151‑1 (MQ=255)

                                                                                 ccGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCagaag                          >  2:5117351/1‑151 (MQ=14)

                                                                                  cGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCagaaga                         <  2:523794/151‑1 (MQ=255)

                                                                                  cGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCagaaga                         <  1:3352301/151‑1 (MQ=255)

                                                                                  cGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCagaaga                         >  1:4761325/1‑151 (MQ=255)

                                                                                  cGCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCagaaga                         <  1:2881621/151‑1 (MQ=255)

                                                                                   gCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAagag                        <  1:2416277/151‑1 (MQ=255)

                                                                                   gCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAagag                        <  2:4104838/151‑1 (MQ=255)

                                                                                   gCACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAagag                        >  1:4201185/1‑151 (MQ=255)

                                                                                    cACGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGt                       <  1:4173601/151‑1 (MQ=255)

                                                                                     aCGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTg                      <  1:4656351/151‑1 (MQ=255)

                                                                                      cGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGa                     <  2:200816/151‑1 (MQ=11)

                                                                                      cGGTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCGCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGa                     <  2:619697/151‑1 (MQ=255)

                                                                                       ggTGTCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGac                    <  2:2619324/151‑2 (MQ=11)

                                                                                          gtCTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCa                 <  2:4020492/151‑1 (MQ=255)

                                                                                            cTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCAcc               <  1:1560693/151‑1 (MQ=11)

                                                                                            cTATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCAcc               >  1:389941/1‑151 (MQ=11)

                                                                                             tATCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCACCg              >  1:140020/1‑151 (MQ=255)

                                                                                              aTCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCACCgg             <  1:1608835/151‑1 (MQ=255)

                                                                                               tCGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCACCGGa            <  2:2697396/151‑1 (MQ=11)

                                                                                                cGAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCACCGGaa           <  2:3861867/151‑1 (MQ=11)

                                                                                                 gAGCTGACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCACCGGAAt          <  1:2694374/151‑1 (MQ=11)

                                                                                                      gACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCACCGGAATACAcc     >  1:4723213/1‑151 (MQ=255)

                                                                                                      gACCACTACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCACCGGAATACAcc     <  2:3095957/151‑1 (MQ=255)

                                                                                                       accactACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCACCGGAATACAccc    <  2:4289386/151‑1 (MQ=11)

                                                                                                       accactACTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCACCGGAATACAccc    >  1:571655/1‑151 (MQ=11)

                                                                                                        ccactaCTGCGCGCGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGCTGTGGTTCAACATCTTATCGCTCAACACGGCCTACAACAGCCTGCAGAAGAGCGACGCACCGGAATACACCCt   >  2:4810637/1‑151 (MQ=11)

CTTTAGCCAGGAGGTGCAGCGGTATTTGAAGAGCGGCATGGCACAGGTTGAAGAGATGAAAGCGTTGCGCATCTCGGGTAGCCGCACGGTGTCTATCGAGCTGACCACCACTGCGCGTGTGAAACCCAACTTCCTTGGGTTGCTCACCTCCGGCGGTGGTGGTGGTTTAAACGCAGGGATGTTGTGGTTCAACATCTTATCGCTCAAGACGGCCTACAACAGCCTGCAGAAGAGTGATGCACCGGAATACACCCTG  >  CP009974/5597013‑5597268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`