Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	775,414	C→T	intergenic (‑176/‑282)	RPPX_03375 ← / → RPPX_03380	16S ribosomal RNA/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	775,414	0	C	T	100.0%	267.8 / NA	91	intergenic (‑176/‑282)	RPPX_03375/RPPX_03380	16S ribosomal RNA/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/41);  total (50/41)

GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGG  >  CP009974/775347‑775561                                                                    |                                                                                                                                                    gTCCGTACTCACAAGCACCCACCCGGATTGCTTGATTCGATTTGTTACAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGCTGTTTTTTACCACCActttc                                                                  <  2:2033429/151‑1 (MQ=11) gTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttc                                                                  >  2:1808348/1‑151 (MQ=255)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                               >  1:3827213/1‑151 (MQ=255)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                               >  1:3557129/1‑151 (MQ=11)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                               >  1:3557103/1‑151 (MQ=11)    cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                               <  1:3369142/151‑1 (MQ=255)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                              <  2:163436/151‑1 (MQ=11)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                              >  1:5176576/1‑151 (MQ=11)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                              <  1:1872039/151‑1 (MQ=11)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                              <  1:4888095/151‑1 (MQ=255)     gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                              >  2:3873663/1‑151 (MQ=255)       aCTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGa                                                            <  1:4827782/151‑1 (MQ=255)        cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                           <  2:2978375/151‑1 (MQ=11)        cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                           <  1:286474/151‑1 (MQ=11)          cacaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAg                                                         >  2:4949463/1‑151 (MQ=255)             aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTTGAAAGCTa                                                      >  1:1258802/1‑151 (MQ=11)             aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                      <  1:4285647/151‑1 (MQ=14)             aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                      >  2:1492833/1‑151 (MQ=255)             aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                      >  2:956401/1‑151 (MQ=255)             aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                      <  1:4347061/151‑1 (MQ=14)              aGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaa                                                     >  2:4294012/1‑151 (MQ=14)              aGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaa                                                     <  2:1999133/151‑1 (MQ=255)               gCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaac                                                    >  2:2433461/1‑151 (MQ=255)               gCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaac                                                    >  1:3262718/1‑151 (MQ=14)                cACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaaca                                                   >  1:2383723/1‑151 (MQ=14)                cACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaaca                                                   >  2:2645132/1‑151 (MQ=14)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                  >  2:5370568/1‑151 (MQ=255)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                  >  2:2449180/1‑151 (MQ=255)                 aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                  >  1:3300091/1‑151 (MQ=255)                   ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                                >  1:2398322/1‑151 (MQ=255)                   ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                                >  1:5151265/1‑151 (MQ=14)                    cacaCGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACtt                                               >  2:211783/1‑151 (MQ=255)                     acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                              >  2:2727718/1‑151 (MQ=14)                     acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                              <  1:521432/151‑1 (MQ=14)                      cacGAATTTCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCTTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTca                                             <  2:4533818/151‑1 (MQ=17)                      cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGACAGCTAACACCTTca                                             >  2:602648/1‑151 (MQ=14)                      cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTca                                             <  2:754746/151‑1 (MQ=255)                      cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTca                                             >  2:974020/1‑151 (MQ=255)                        cGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaac                                           <  1:3034971/151‑1 (MQ=14)                         gAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaaca                                          >  1:1518568/1‑151 (MQ=255)                         gAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaaca                                          <  2:4209429/151‑1 (MQ=17)                          aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                         <  1:1359650/151‑1 (MQ=17)                            ttGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACGTCAACAACt                                       <  1:3562387/151‑1 (MQ=17)                             tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                      >  2:3312350/1‑151 (MQ=255)                             tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                      >  2:3774604/1‑151 (MQ=255)                             tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                      >  2:3776793/1‑151 (MQ=17)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                     <  1:2139170/151‑1 (MQ=17)                              gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                     >  1:4506359/1‑151 (MQ=255)                               cTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAc                                    <  2:1408363/151‑1 (MQ=255)                                ttGATTCGATTTGTTAAGGAGCGGTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                   <  2:3924191/151‑1 (MQ=17)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                   <  2:3278455/151‑1 (MQ=255)                                ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                   <  2:2865806/151‑1 (MQ=255)                                     tCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTg                              <  1:2535980/151‑1 (MQ=255)                                      cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGc                             >  1:4747930/1‑151 (MQ=18)                                      cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGc                             <  1:4001725/151‑1 (MQ=18)                                      cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGc                             <  2:507292/151‑1 (MQ=18)                                      cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGc                             <  2:1094388/151‑1 (MQ=255)                                      cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCGTgg                             <  1:2433461/151‑2 (MQ=17)                                       gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                            <  1:3223923/151‑1 (MQ=18)                                         tttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcccc                          <  1:3568509/151‑1 (MQ=18)                                         tttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcccc                          <  1:2093896/151‑1 (MQ=18)                                         tttGCTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGAATATTTTGAAGTTTTTTCCCACCACTTTCTTTGGAACGCGAACTGCCTCAACACCTTCCCGCCTGcccc                          >  2:4219083/1‑151 (MQ=14)                                          ttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCg                         <  1:2801204/151‑1 (MQ=18)                                           ttttAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                        >  1:351894/3‑151 (MQ=18)                                           tGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                        >  2:124900/1‑151 (MQ=18)                                           tGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                        <  2:2892332/151‑1 (MQ=255)                                              tAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGc                     >  1:2327805/1‑151 (MQ=18)                                               aaaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCg                    <  2:3105754/151‑1 (MQ=255)                                               aaaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCg                    >  1:945753/1‑151 (MQ=255)                                               aaaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCg                    >  1:4000820/1‑151 (MQ=18)                                                   agCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCa                <  2:1255541/151‑1 (MQ=255)                                                    gCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCAt               >  2:4318548/1‑151 (MQ=21)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg             <  2:3916050/151‑1 (MQ=255)                                                      gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg             <  2:4673193/151‑1 (MQ=255)                                                       tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                        <  2:2279048/139‑1 (MQ=18)                                                       tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                        >  1:2279048/1‑139 (MQ=18)                                                       tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGc            >  2:4070985/1‑151 (MQ=21)                                                        ttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCt           >  2:2103602/1‑151 (MQ=255)                                                          ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGc         >  1:2451287/1‑151 (MQ=21)                                                           gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg        >  1:4785285/1‑151 (MQ=25)                                                           gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg        <  2:1517296/151‑1 (MQ=255)                                                            ttAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTTCga       >  2:1743011/1‑151 (MQ=21)                                                             tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag      <  1:5370568/151‑1 (MQ=255)                                                             tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag      <  1:135995/151‑1 (MQ=25)                                                             tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag      >  2:4123299/1‑151 (MQ=255)                                                             tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTCTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACCTACCGCTTGCCCCGAAGCGCTCATCGCTGCgag      >  2:2638106/1‑151 (MQ=21)                                                              aaGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgagg     >  2:2755091/1‑151 (MQ=255)                                                                gagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgaggag   >  2:2805843/1‑151 (MQ=255)                                                                gagCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTCCgaggag   >  2:4641937/1‑151 (MQ=25)                                                                 agCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGaggagg  >  1:639967/1‑151 (MQ=255)                                                                 agCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGagcagg  >  2:336965/1‑151 (MQ=255)                                                                    |                                                                                                                                                    GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGG  >  CP009974/775347‑775561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑