Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 775,456 G→A intergenic (‑218/‑240) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009974775,4560GA100.0% 530.7 / NA 164intergenic (‑218/‑240)RPPX_03375/RPPX_0338016S ribosomal RNA/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (80/84);  total (80/84)

Only 100 of 165 total aligned reads displayed.

GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCT  >  CP009974/775347‑775603
                                                                                                             |                                                                                                                                                   
   cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                                                                         >  1:3827213/1‑151 (MQ=255)
   cGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCActttcttt                                                                                                         >  1:3557129/1‑151 (MQ=11)
    gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                                                                        >  2:3873663/1‑151 (MQ=255)
    gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                                                                        >  1:5176576/1‑151 (MQ=11)
    gTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACtttctttc                                                                                                        <  1:4888095/151‑1 (MQ=255)
      aCTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGa                                                                                                      <  1:4827782/151‑1 (MQ=255)
       cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                                                                     <  2:2978375/151‑1 (MQ=11)
       cTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGaa                                                                                                     <  1:286474/151‑1 (MQ=11)
         cacaAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAg                                                                                                   >  2:4949463/1‑151 (MQ=255)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTTGAAAGCTa                                                                                                >  1:1258802/1‑151 (MQ=11)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                                                                >  2:956401/1‑151 (MQ=255)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                                                                >  2:1492833/1‑151 (MQ=255)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                                                                <  1:4347061/151‑1 (MQ=14)
            aaGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTa                                                                                                <  1:4285647/151‑1 (MQ=14)
             aGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaa                                                                                               <  2:1999133/151‑1 (MQ=255)
             aGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaa                                                                                               >  2:4294012/1‑151 (MQ=14)
              gCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaac                                                                                              >  1:3262718/1‑151 (MQ=14)
                aCCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTaacaa                                                                                            >  2:2449180/1‑151 (MQ=255)
                  ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                                                                          >  1:5151265/1‑151 (MQ=14)
                  ccACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACt                                                                                          >  1:2398322/1‑151 (MQ=255)
                    acacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTc                                                                                        >  2:2727718/1‑151 (MQ=14)
                     cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGACAGCTAACACCTTca                                                                                       >  2:602648/1‑151 (MQ=14)
                     cacGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTca                                                                                       <  2:754746/151‑1 (MQ=255)
                       cGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaac                                                                                     <  1:3034971/151‑1 (MQ=14)
                        gAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaaca                                                                                    <  2:4209429/151‑1 (MQ=17)
                        gAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaaca                                                                                    >  1:1518568/1‑151 (MQ=255)
                         aaTTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTcaacaa                                                                                   <  1:1359650/151‑1 (MQ=17)
                           ttGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACGTCAACAACt                                                                                 <  1:3562387/151‑1 (MQ=17)
                            tGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACtt                                                                                >  2:3774604/1‑151 (MQ=255)
                             gCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTa                                                                               <  1:2139170/151‑1 (MQ=17)
                               ttGATTCGATTTGTTAAGGAGCGGTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                                                             <  2:3924191/151‑1 (MQ=17)
                               ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                                                             <  2:3278455/151‑1 (MQ=255)
                               ttGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTAcc                                                                             <  2:2865806/151‑1 (MQ=255)
                                     cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGc                                                                       <  2:1094388/151‑1 (MQ=255)
                                     cGATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGc                                                                       <  1:4001725/151‑1 (MQ=18)
                                      gATTTGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcc                                                                      <  1:3223923/151‑1 (MQ=18)
                                        tttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGcccc                                                                    <  1:2093896/151‑1 (MQ=18)
                                         ttGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCg                                                                   <  1:2801204/151‑1 (MQ=18)
                                          ttttAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                                                                  >  1:351894/3‑151 (MQ=18)
                                          tGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                                                                  <  2:2892332/151‑1 (MQ=255)
                                          tGTTAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                                                                  >  2:124900/1‑151 (MQ=18)
                                             tAAAGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGc                                                               >  1:2327805/1‑151 (MQ=18)
                                              aaaGAGCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCg                                                              <  2:3105754/151‑1 (MQ=255)
                                                  agCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCa                                                          <  2:1255541/151‑1 (MQ=255)
                                                   gCGTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCAt                                                         >  2:4318548/1‑151 (MQ=21)
                                                     gTTTGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCg                                                       <  2:4673193/151‑1 (MQ=255)
                                                      tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                                                                  <  2:2279048/139‑1 (MQ=18)
                                                      tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGa                                                                  >  1:2279048/1‑139 (MQ=18)
                                                      tttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGc                                                      >  2:4070985/1‑151 (MQ=21)
                                                       ttGGTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCt                                                     >  2:2103602/1‑151 (MQ=255)
                                                         ggTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGc                                                   >  1:2451287/1‑151 (MQ=21)
                                                          gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg                                                  <  2:1517296/151‑1 (MQ=255)
                                                          gTTAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCg                                                  >  1:4785285/1‑151 (MQ=25)
                                                           ttAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTTCga                                                 >  2:1743011/1‑151 (MQ=21)
                                                            tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCgag                                                <  1:5370568/151‑1 (MQ=255)
                                                            tAAGAGCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTCTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACCTACCGCTTGCCCCGAAGCGCTCATCGCTGCgag                                                >  2:2638106/1‑151 (MQ=21)
                                                                agCTTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGaggagg                                            >  1:639967/1‑151 (MQ=255)
                                                                 gcttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGc                                           <  2:552335/148‑1 (MQ=255)
                                                                 gcttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGc                                           >  1:5324987/4‑151 (MQ=255)
                                                                 gcttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGc                                           <  2:818881/148‑1 (MQ=255)
                                                                  cttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCg                                          <  2:136962/149‑1 (MQ=255)
                                                                  cttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCg                                          <  1:4538641/149‑1 (MQ=255)
                                                                   ttttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGa                                         >  1:858801/2‑151 (MQ=255)
                                                                     ttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaat                                       <  2:35875/151‑1 (MQ=255)
                                                                     ttCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaat                                       <  1:761905/151‑1 (MQ=255)
                                                                        gTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGaataat                                    >  1:1780214/1‑151 (MQ=255)
                                                                         tctcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAataata                                   <  2:4865303/151‑1 (MQ=255)
                                                                         tctcAACCGACGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAataata                                   <  2:639967/151‑1 (MQ=255)
                                                                          ctcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAc                                  <  2:4584217/151‑1 (MQ=255)
                                                                          ctcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAc                                  <  1:1426843/151‑1 (MQ=255)
                                                                           tcAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcg                                 >  2:4227905/1‑151 (MQ=255)
                                                                            cAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcgc                                >  1:1327565/1‑151 (MQ=255)
                                                                            cAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcgc                                >  1:3714656/1‑151 (MQ=255)
                                                                            cAACCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcgc                                >  1:3714641/1‑151 (MQ=255)
                                                                             aaCCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATAcgcg                               >  1:632911/1‑151 (MQ=255)
                                                                              aCCGAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACgcgc                              <  1:1140990/151‑1 (MQ=255)
                                                                                 gAGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCttt                           <  1:2441750/151‑1 (MQ=255)
                                                                                  aGGCGCGCATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCAATTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTg                          <  2:5192182/151‑1 (MQ=255)
                                                                                        gcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCa                    <  2:3060149/151‑1 (MQ=255)
                                                                                        gcATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCa                    >  1:229059/1‑151 (MQ=255)
                                                                                        gcATTCTACGATTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAATCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCa                    <  1:4093693/151‑1 (MQ=38)
                                                                                         cATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCag                   <  2:2804301/151‑1 (MQ=255)
                                                                                         cATTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCag                   <  2:2324614/151‑1 (MQ=255)
                                                                                          aTTCTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCaga                  >  1:894684/1‑151 (MQ=255)
                                                                                             cTACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTc               <  1:3645013/151‑1 (MQ=255)
                                                                                              tACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCCCCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCa              <  1:5306867/151‑1 (MQ=255)
                                                                                              tACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGccc                                                                     >  1:2608019/1‑96 (MQ=37)
                                                                                              tACGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCa              <  2:3352748/151‑1 (MQ=255)
                                                                                               aCGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCaa             <  2:272196/151‑1 (MQ=255)
                                                                                               aCGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCaa             <  2:1852330/151‑1 (MQ=255)
                                                                                                cGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAac            <  1:3367880/151‑1 (MQ=255)
                                                                                                cGCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAac            <  2:2513579/151‑1 (MQ=255)
                                                                                                 gCTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAaca           >  1:440559/1‑151 (MQ=255)
                                                                                                  cTTTCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAacac          <  2:3184967/151‑1 (MQ=255)
                                                                                                    ttCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCt        >  2:5096400/1‑151 (MQ=255)
                                                                                                    ttCCTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGCCAACACCt        >  2:3285289/1‑151 (MQ=255)
                                                                                                      ccTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTg      >  1:2148477/1‑151 (MQ=255)
                                                                                                       cTCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGt     <  2:4188778/151‑1 (MQ=255)
                                                                                                        tCAGAACCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGtt    <  2:883320/151‑1 (MQ=255)
                                                                                                          agaaCCTGTCAAGCGTTTATTTTGAAGTTTTTTACCACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCt  >  1:334035/1‑151 (MQ=255)
                                                                                                             |                                                                                                                                                   
GTCCGTACTCACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGGTTAAGAGCCTTTCGTCTCAACCGAGGCGCGCATTCTACGCTTTCCTCAGAGCCTGTCAAGCGTTTATTTTGAAGTTTTTTGCGACCACTTTCTTTCGAAAGCTAACAACTTCAACAACTTACCGCTTGCCCCGAAGCGTTCATCGCTGCGAGGAGGCGAATAATACGCGCTTTGAAATCAGAGTCAACACCTTGTTCT  >  CP009974/775347‑775603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑