Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,154,311	T→C	T337T (ACT→ACC)	RPPX_05115 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,154,311	0	T	C	100.0%	24.0 / NA	15	T337T (ACT→ACC)	RPPX_05115	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/10);  total (5/10)

AGTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACTGACCTGGCCCT  >  CP009974/1154163‑1154322                                                                                                                                                     |            aGTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAAccgaccga           >  1:3058357/1‑151 (MQ=37) aGTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAAccgaccga           <  2:2445971/151‑1 (MQ=25) aGTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAAccgaccga           <  1:686344/151‑1 (MQ=37)  gTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACcgaccgac          >  2:2331050/1‑151 (MQ=25)   ttCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCgaccgacc         <  1:2494201/151‑1 (MQ=21)    tCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcct        <  1:3291464/151‑1 (MQ=18)     cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       >  1:1714114/1‑151 (MQ=18)     cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       >  2:5011161/1‑151 (MQ=18)     cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  2:4973851/151‑1 (MQ=18)     cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  1:5228705/151‑1 (MQ=18)     cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctg       <  1:1487266/151‑1 (MQ=18)     cAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctc       >  2:3502511/1‑150 (MQ=18)      aGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCTCCTGAACAACCGACCGAcctgg      <  2:4832309/151‑1 (MQ=17)       gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     <  1:3789190/151‑1 (MQ=17)       gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     >  2:3985186/1‑151 (MQ=14)       gCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggc     <  2:5371083/151‑1 (MQ=14)        cATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggcc    <  1:334860/151‑1 (MQ=14)          ttACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCCGGCAATAGCTCGCCAGTTGCCAACCTCACGGCACCGGACGTCGCCACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccct  <  2:5171744/151‑1 (MQ=2)          ttACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGCCACCCCTGATACTACAGCCCCTCAACCACCGCCCGAcctggcccc  >  2:4906750/1‑150 (MQ=11)          ttACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACCGAcctggccct  <  2:4618277/151‑1 (MQ=11)                                                                                                                                                     |            AGTTCAGCATTACCCTGCAACCGCCACAAAGCGACGGCCAGGCCCTGGAGGTCAGCGCCGCAGATGCGGCAGGCAATAGCTCGCCAGTTGCCAACATCACGGCACCGGACGTCGACACCCCTGATACTACAGCCCCTGAACAACCGACTGACCTGGCCCT  >  CP009974/1154163‑1154322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑