Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,155,739 G→A P813P (CCG→CCA)  RPPX_05115 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,155,7390GA99.1% 810.7 / NA 219P813P (CCG→CCA) RPPX_05115hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (92/125);  total (94/125)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GATACGTTGCCGGCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCATTGATCACCAGCTCGGTCGGTGCGGCAGGGGCGGTGATGTCGGGCGCGGTGGTCAATGGTACGTCGTACTCGGTAGCCACCGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCC  >  CP009974/1155653‑1155886
                                                                                      |                                                                                                                                                   
gagctgttaccGGCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcac                                                                                     <  2:1700536‑M2/146‑59 (MQ=255)
  gctgttaCCGGCGGCATCCACCAGACGCACACCGAGTGCTTCACCAGTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccg                                                                                   >  2:1149366‑M2/4‑91 (MQ=255)
   ctgttACCGGCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgg                                                                                  <  2:2133442‑M2/149‑62 (MQ=255)
    tggtaccggcggCATCCACCAGGCGCACATCGAGTGCTTCACCGTTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccggg                                                                                 >  2:1111607‑M2/6‑89 (MQ=255)
     gTTACCGGCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggc                                                                                <  2:1995423‑M2/151‑64 (MQ=255)
      ttCCCGGCGGCATCCCGCAGACCCACGTCGAGTGCTTCGCCCGTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggct                                                                               >  2:3808853‑M2/1‑87 (MQ=255)
      ttACCGGCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggcc                                                                               <  2:237513‑M2/151‑65 (MQ=255)
       taccggCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcgccgggctc                                                                              <  2:3326872‑M2/149‑66 (MQ=255)
       taccggCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctc                                                                              <  2:2797608‑M2/149‑66 (MQ=255)
       taccggCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctc                                                                              >  2:2296939‑M2/3‑86 (MQ=255)
       taccggCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctc                                                                              >  1:2281906‑M2/3‑86 (MQ=255)
       taccggCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctc                                                                              <  1:802850‑M2/149‑66 (MQ=255)
          cggcggCGTCCGCCAGAGGCACATCCAGTGCTTCACCATGGGCCTGGGCAGGCGAGAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacgccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgcc                                                                           >  2:3285578‑M2/1‑83 (MQ=255)
          cggcggCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGTCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgcc                                                                           <  1:130576‑M2/151‑69 (MQ=255)
           ggcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccg                                                                          >  1:347342‑M2/1‑82 (MQ=255)
           ggcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccg                                                                          <  1:3946535‑M2/151‑70 (MQ=255)
           ggcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccg                                                                          >  1:4881930‑M2/1‑82 (MQ=255)
           ggcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccg                                                                          >  2:2686674‑M2/1‑82 (MQ=255)
           ggcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccc                                                                          <  1:3917886‑M2/151‑70 (MQ=255)
           ggcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggcgcgccg                                                                          <  2:1371375‑M2/151‑70 (MQ=255)
            gcggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgcccc                                                                         <  2:3116502‑M2/151‑71 (MQ=255)
             cggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcg                                                                        <  2:901775‑M2/151‑72 (MQ=255)
             cggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcg                                                                        <  1:4529172‑M2/151‑72 (MQ=255)
             cggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcg                                                                        <  1:1514131‑M2/151‑72 (MQ=255)
              ggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcgg                                                                       <  2:1789055‑M2/151‑73 (MQ=255)
              ggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggccgcagcatcgcgcacctgcacggtagcaccgggctcgccgcgg                                                                       <  2:4147964‑M2/151‑73 (MQ=255)
              ggcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggggccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcgg                                                                       <  2:2112377‑M2/151‑73 (MQ=255)
               gcATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggc                                                                      <  2:1405448‑M2/151‑74 (MQ=255)
                cATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggcc                                                                     <  1:299842‑M2/151‑75 (MQ=255)
                cATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggcc                                                                     >  1:2158771‑M2/1‑77 (MQ=255)
                  tCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccgg                                                                   >  2:4356649‑M2/1‑75 (MQ=255)
                   ccaccaGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggt                                                                  <  1:1910267‑M2/151‑78 (MQ=255)
                   ccaccaGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCGGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgcgctcgccgcggccggt                                                                  <  1:2565631‑M2/151‑78 (MQ=255)
                     accaGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtga                                                                <  1:1243536‑M2/151‑80 (MQ=255)
                       caGACGCACGTCGAGTGCTTCACCAGTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaag                                                              >  2:186238‑M2/1‑70 (MQ=255)
                       caGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaag                                                              >  1:2181150‑M2/1‑70 (MQ=255)
                       caGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaag                                                              >  1:1701160‑M2/1‑70 (MQ=255)
                         ggcgcACATCGAGTGCTTCACCAGTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggt                                                            >  2:4340028‑M2/3‑68 (MQ=255)
                         gACGGACATCGAGTGCTTCCCCATGGGCGTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggt                                                            >  2:3620744‑M2/1‑68 (MQ=255)
                         gACGCACATCGGGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggt                                                            >  2:2097522‑M2/1‑68 (MQ=255)
                         gACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggt                                                            <  1:2304971‑M2/151‑84 (MQ=255)
                          aCGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtg                                                           >  2:5401402‑M2/1‑67 (MQ=255)
                            gCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgac                                                         >  1:3198707‑M2/1‑65 (MQ=255)
                             cacaTCGAGTGCTTCACCGTTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacg                                                        >  2:2368850‑M2/1‑64 (MQ=255)
                              acaTCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgc                                                       <  1:5252500‑M2/151‑89 (MQ=255)
                               caTCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgcc                                                      >  1:4796229‑M2/1‑62 (MQ=255)
                               caTCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgcc                                                      <  1:1588614‑M2/151‑90 (MQ=255)
                                 tCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccat                                                    <  1:3802342‑M2/151‑92 (MQ=255)
                                  cGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatc                                                   <  1:1170142‑M2/151‑93 (MQ=255)
                                  cGAGTGCGTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatc                                                   >  2:3169549‑M2/1‑59 (MQ=255)
                                   gAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcg                                                  >  1:5047351‑M2/1‑58 (MQ=255)
                                   gAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacagtagcaccgggctcgccgccgccggggaaggtgacgccatcg                                                  <  2:855417‑M2/151‑94 (MQ=255)
                                    aGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcgg                                                 <  1:3997952‑M2/151‑95 (MQ=255)
                                      tGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggcc                                               >  1:182638‑M2/1‑55 (MQ=255)
                                      tGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcgccc                                               <  2:785740‑M2/151‑97 (MQ=255)
                                       gCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggcca                                              >  2:4210198‑M2/1‑54 (MQ=255)
                                        cTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaa                                             <  1:557910‑M2/151‑99 (MQ=255)
                                         ttCGCCAGTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagg                                            >  2:4583399‑M2/1‑52 (MQ=255)
                                         ttCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaag                                            >  1:564454‑M2/1‑52 (MQ=255)
                                          tCACCATTGGCTTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaggg                                           >  1:3573884‑M2/1‑51 (MQ=255)
                                          tCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaagg                                           >  1:3363599‑M2/1‑51 (MQ=255)
                                           cacccggggccggggcAGGCGCGAGGGTGAGGCGGAACAGGCCATCGGCAccgacgacgccggtgccgatcaggttggcggcggcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaaggc                                          >  2:3726380‑M2/8‑50 (MQ=255)
                                           cACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggc                                          <  1:4211131‑M2/151‑102 (MQ=255)
                                            aCCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaaggcc                                         <  2:2568278‑M2/151‑103 (MQ=255)
                                             ccATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaaggcca                                        <  1:3854303‑M2/151‑104 (MQ=255)
                                              cATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaa                                       <  1:114075‑M2/151‑105 (MQ=255)
                                              cAGTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccaaggccag                                       >  2:3034152‑M2/1‑47 (MQ=255)
                                                ttGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggcgcgccgcggccggtgaaggtgacgccatcggccaaggccaggt                                     <  2:679543‑M2/151‑107 (MQ=255)
                                                 tGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtc                                    <  1:3684201‑M2/151‑108 (MQ=255)
                                                       gggCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcgg                              <  2:2560097‑M2/151‑114 (MQ=255)
                                                       gggCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcgg                              <  1:1065236‑M2/151‑114 (MQ=255)
                                                        ggCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtggcgccatcggccagggccaagtcactcggt                             <  1:922858‑M2/151‑115 (MQ=255)
                                                         gCAGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggtt                            >  1:976895‑M2/1‑36 (MQ=255)
                                                           aGGCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgt                          <  1:5303236‑M2/151‑118 (MQ=255)
                                                             gCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgc                        >  1:2537557‑M2/1‑32 (MQ=255)
                                                             gCGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcaggcggttgtgc                        <  1:1390481‑M2/151‑120 (MQ=255)
                                                              cGACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgaccaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccgctgatggtgacgcccacgaccagggcaatgtcagtccgttgtgcg                       <  2:1047987‑M2/151‑121 (MQ=255)
                                                               gACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgg                      <  1:3141338‑M2/151‑122 (MQ=255)
                                                               gACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgg                      <  1:5211654‑M2/151‑122 (MQ=255)
                                                               gACAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgg                      >  1:4166593‑M2/1‑30 (MQ=255)
                                                                 cAGGGTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggc                    <  1:2021715‑M2/151‑124 (MQ=255)
                                                                   gggTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcgg                  <  1:2522211‑M2/151‑126 (MQ=255)
                                                                   gggTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcgg                  >  2:577188‑M2/1‑26 (MQ=255)
                                                                    ggTGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggc                 >  2:2248875‑M2/1‑25 (MQ=255)
                                                                     gcgagGCTGGACAGGCCATCGGCAccgacgacaccggtgccgatcagggtgggggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggca                >  2:1500027‑M2/3‑24 (MQ=255)
                                                                     gTGAGGCTGAACAGGCCGTCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggca                >  2:4798247‑M2/1‑24 (MQ=255)
                                                                      tGAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcag               <  1:3286073‑M2/151‑129 (MQ=255)
                                                                       gAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagc              >  2:1208731‑M2/1‑22 (MQ=255)
                                                                       gAGGCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcgggtgtgcgggcggcagc              <  1:4390106‑M2/151‑130 (MQ=255)
                                                                         ggCTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggcgccgccatcggccagggccaagtcagtcggatgtgcggggggcagcaa            <  2:278265‑M2/151‑132 (MQ=255)
                                                                           cTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagt          >  1:5016629‑M2/1‑18 (MQ=255)
                                                                           cTGAACAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagt          >  1:963136‑M2/1‑18 (MQ=255)
                                                                              aaCAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcgg       <  1:2861081‑M2/151‑137 (MQ=255)
                                                                               aCAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcggc      >  1:4572616‑M2/1‑14 (MQ=255)
                                                                                cAGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccacggccaagccagtcggttgtgcgggcggcagcaagtcggcc     <  2:5044614‑M2/151‑139 (MQ=255)
                                                                                 aGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcggccg    <  1:4596935‑M2/151‑140 (MQ=255)
                                                                                 aGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcggccg    <  1:3973380‑M2/151‑140 (MQ=255)
                                                                                 aGGCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcggccg    <  1:2987259‑M2/151‑140 (MQ=255)
                                                                                   gCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtagcaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcggccgaa  <  2:5238826‑M2/151‑142 (MQ=255)
                                                                                   gCCATCGGCAccgacgacaccggtgccgatcaggttggcggcagcatcgcgcacctgcacggtaacaccgggctcgccgcggccggtgaaggtgacgccatcggccagggccaagtcagtcggttgtgcgggcggcagcaagtcggccgaa  >  2:5211654‑M2/1‑10 (MQ=255)
                                                                                      |                                                                                                                                                   
GATACGTTGCCGGCGGCATCCACCAGACGCACATCGAGTGCTTCACCATTGGCCTGGGCAGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCATTGATCACCAGCTCGGTCGGTGCGGCAGGGGCGGTGATGTCGGGCGCGGTGGTCAATGGTACGTCGTACTCGGTAGCCACCGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCC  >  CP009974/1155653‑1155886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑