Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,155,982 C→T T23S (ACC→TCT)  RPPX_05120 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,155,9820CT99.0% 1085.8 / ‑3.2 295T23S (ACC→TCT) RPPX_05120hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (133/159);  total (136/159)
Fisher's exact test for biased strand distribution p-value = 9.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 295 total aligned reads displayed.

CGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCCGGGGCGAGATCGATCAGGAAGGTGCCGTTGGCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCAGCGTCACGCTGTTCCCCGCC  >  CP009974/1155837‑1156072
                                                                                                                                                 |                                                                                          
ccgatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggcgatcggcgcagccggcagcccgctcagcggtcgaggcgaacccGGCGCTACCGt                                                                                       <  2:2771496‑M1/11‑1 (MQ=255)
 cgatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTg                                                                                      >  1:4127443‑M1/140‑151 (MQ=255)
  gatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGc                                                                                     <  2:3334424‑M1/13‑1 (MQ=255)
  gatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGc                                                                                     <  2:5321748‑M1/13‑1 (MQ=255)
  gatgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacagcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggccgccagctcagcggtcgaggcgaagccGGCTCTACCGTGc                                                                                     <  2:4588589‑M1/13‑1 (MQ=255)
   atgcggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcgggcgaggcgaagccGGCTCTACCGTgcc                                                                                    <  2:1500853‑M1/14‑2 (MQ=255)
      cggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGt                                                                                 <  2:4965356‑M1/17‑1 (MQ=255)
      cggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGt                                                                                 >  2:2367644‑M1/135‑151 (MQ=255)
       ggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTg                                                                                <  2:3881472‑M1/18‑1 (MQ=255)
       ggcaggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTg                                                                                >  1:1688745‑M1/134‑151 (MQ=255)
         caggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCg                                                                              >  1:2279716‑M1/132‑151 (MQ=255)
          aggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGt                                                                             <  1:2568278‑M1/21‑1 (MQ=255)
           ggcaacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTg                                                                            >  2:4824292‑M1/130‑151 (MQ=255)
             caacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAc                                                                          >  2:1015102‑M1/128‑151 (MQ=255)
              aacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcg                                                                         <  1:4733136‑M1/25‑1 (MQ=255)
              aacgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGAcg                                                                         <  1:33037‑M1/25‑1 (MQ=255)
               acgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGacgt                                                                        <  1:4888218‑M1/26‑2 (MQ=255)
               acgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGacgt                                                                        <  1:679543‑M1/26‑2 (MQ=255)
                cgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTgacgtg                                                                       <  2:17129‑M1/27‑3 (MQ=255)
                cgcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTgacgtg                                                                       <  1:4868134‑M1/27‑3 (MQ=255)
                 gcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGtgacgtgg                                                                      >  2:155459‑M1/124‑148 (MQ=255)
                 gcctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGcgg                                                                      <  1:3113352‑M1/28‑1 (MQ=255)
                  cctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGcggc                                                                     >  1:3691411‑M1/123‑151 (MQ=255)
                   ctccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACgtggca                                                                    <  1:428185‑M1/30‑2 (MQ=255)
                     ccaccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcgttcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGacgtggcagg                                                                  <  2:223079‑M1/32‑4 (MQ=255)
                      caccgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGtggcaggc                                                                 <  1:2192633‑M1/33‑1 (MQ=255)
                       accgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTggcaggca                                                                <  2:561802‑M1/34‑1 (MQ=255)
                         cgccaataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAAc                                                              >  2:377193‑M1/116‑151 (MQ=255)
                          gccaataaccgtaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACa                                                             >  1:4765596‑M1/115‑151 (MQ=255)
                            caataaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATc                                                           >  1:2483745‑M1/113‑151 (MQ=255)
                               taaccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTc                                                        >  1:4660661‑M1/110‑151 (MQ=255)
                                 accttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTcgc                                                      >  1:2729773‑M1/108‑151 (MQ=255)
                                 accttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTcgc                                                      >  2:3861799‑M1/108‑151 (MQ=255)
                                  ccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTcgcg                                                     >  1:2166725‑M1/107‑151 (MQ=255)
                                  ccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTcgcg                                                     <  1:3463447‑M1/45‑1 (MQ=255)
                                  ccttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTcgcg                                                     <  1:2839006‑M1/45‑1 (MQ=255)
                                   cttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGa                                                    >  1:5424117‑M1/106‑151 (MQ=255)
                                   cttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGa                                                    <  1:1598851‑M1/46‑1 (MQ=255)
                                    ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGAc                                                   <  1:1427751‑M1/47‑1 (MQ=255)
                                    ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGAc                                                   >  1:1091752‑M1/105‑151 (MQ=255)
                                    ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGAc                                                   >  2:3143241‑M1/105‑151 (MQ=255)
                                    ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGCGACGCCGCCGGCACCATCCTCGCGAc                                                   <  2:1971498‑M1/47‑1 (MQ=255)
                                    ttaccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctcgtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGAAACATcctcgccac                                                   <  2:4206230‑M1/47‑4 (MQ=255)
                                     taccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGAcc                                                  >  1:810352‑M1/104‑151 (MQ=255)
                                      accgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCg                                                 >  1:2941939‑M1/103‑151 (MQ=255)
                                      accgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCg                                                 <  1:5168875‑M1/49‑1 (MQ=255)
                                      accgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCg                                                 >  2:3050803‑M1/103‑151 (MQ=255)
                                       ccgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCgg                                                <  1:3334222‑M1/50‑1 (MQ=255)
                                        cgcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGc                                               <  2:3594561‑M1/51‑1 (MQ=255)
                                         gcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCa                                              <  1:1018847‑M1/52‑1 (MQ=255)
                                         gcgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGAGACGCGGCCGGCAACATCCTCGCGACCGGCa                                              <  1:2431514‑M1/52‑1 (MQ=255)
                                          cgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCAc                                             <  1:4940349‑M1/53‑1 (MQ=255)
                                          cgccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCGCTACCGTGCAGGTGCGCCACGCCGCCGGCCACCCCCTCCCGACCCGCAc                                             <  2:4062188‑M1/53‑1 (MQ=255)
                                           gccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCAcc                                            >  1:1961903‑M1/98‑151 (MQ=255)
                                           gccggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACCTCCGCGCGACCGGCAcc                                            <  2:4488700‑M1/54‑1 (MQ=255)
                                             cggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGt                                          <  2:1871696‑M1/56‑1 (MQ=255)
                                              ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTc                                         >  2:4398039‑M1/95‑151 (MQ=255)
                                              ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTc                                         >  1:92388‑M1/95‑151 (MQ=255)
                                              ggacatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTc                                         >  2:5147195‑M1/95‑151 (MQ=255)
                                               gacctcgacggcggcgacacatcgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagcgcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCg                                        >  2:1278464‑M1/94‑151 (MQ=255)
                                                acatcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagcacagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGGCGTGGCAGGCAGCATCCTCGGGCGCGGCACCGTCGc                                       <  1:5039003‑M1/59‑1 (MQ=255)
                                                  atcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCTGGCAACATCCTCGCGACCGGCACCGTCGCTg                                     <  2:2656670‑M1/61‑1 (MQ=255)
                                                  atcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTg                                     <  1:1535344‑M1/61‑1 (MQ=255)
                                                  atcgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTg                                     >  2:1598851‑M1/91‑151 (MQ=255)
                                                    cgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCa                                   >  1:1416077‑M1/89‑151 (MQ=255)
                                                    cgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCa                                   <  2:883266‑M1/63‑1 (MQ=255)
                                                    cgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCa                                   >  1:1319185‑M1/89‑151 (MQ=255)
                                                    cgacggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGCGCGTGACGCCGCCGGCAACATCCTCGCGACCGGCACCGTcgcttcc                                   <  2:3947877‑M1/63‑4 (MQ=255)
                                                     gacggcggcgccacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAg                                  >  2:2127159‑M1/88‑151 (MQ=255)
                                                       cggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGAt                                >  1:1851584‑M1/86‑151 (MQ=255)
                                                        gggggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                               >  1:738470‑M1/85‑151 (MQ=255)
                                                        ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                               >  1:3415312‑M1/85‑151 (MQ=255)
                                                        ggcggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATg                               <  1:4581951‑M1/67‑1 (MQ=255)
                                                          cggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCCGATGGc                             <  1:3848127‑M1/69‑1 (MQ=255)
                                                           ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCCCCGTCGCTGCAGATGgcc                            <  1:3874233‑M1/70‑2 (MQ=255)
                                                           ggcgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                            >  1:40580‑M1/82‑151 (MQ=255)
                                                           ggcgacacaacgccaccggaggccccgacaaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCa                            >  1:1240036‑M1/82‑151 (MQ=255)
                                                             cgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCAcc                          <  1:1760633‑M1/72‑1 (MQ=255)
                                                             cgacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCAcc                          >  2:2194346‑M1/80‑151 (MQ=255)
                                                              gacacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCt                         >  1:299453‑M1/79‑151 (MQ=255)
                                                               acacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCtt                        >  1:2799709‑M1/78‑151 (MQ=255)
                                                                cacaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTc                       >  1:3243990‑M1/77‑151 (MQ=255)
                                                                 acaacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCa                      >  2:353985‑M1/76‑151 (MQ=255)
                                                                   aacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTtcacc                    <  1:5305369‑M1/78‑3 (MQ=255)
                                                                   aacgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTtcacc                    <  1:2046069‑M1/78‑3 (MQ=255)
                                                                    gcgccaccggaggccgcgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCttcacca                   >  2:4887913‑M1/73‑148 (MQ=255)
                                                                     cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                  >  1:5247617‑M1/72‑147 (MQ=255)
                                                                     cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                  >  2:1184639‑M1/72‑147 (MQ=255)
                                                                     cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                  <  1:5112772‑M1/80‑5 (MQ=255)
                                                                     cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                  >  2:2564794‑M1/72‑147 (MQ=255)
                                                                     cgccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggagaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACcttcaccat                  >  1:1036237‑M1/72‑147 (MQ=255)
                                                                       ccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCaccttcaccatca                <  1:782521‑M1/82‑7 (MQ=255)
                                                                       ccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCaccttcaccatca                <  1:4485805‑M1/82‑7 (MQ=255)
                                                                       ccaccggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCaccttcaccatca                >  2:2933578‑M1/70‑145 (MQ=255)
                                                                           cggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCACCAtcacccc            <  2:4622063‑M1/86‑4 (MQ=255)
                                                                            ggaggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCCCCTTCACCATCACCCTg           <  2:673589‑M1/87‑1 (MQ=255)
                                                                              aggccccgaccaatctggtgatcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCACCATCACCctggc         <  2:5387323‑M1/89‑3 (MQ=255)
                                                                               agCACCGACCAATCTGGGGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGGCGCTTCAGAGGGCACCTTCCTCATCACCCTggcc        >  2:802850/2‑148 (MQ=14)
                                                                                     gACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCAGCTTCGTCATCGCCCTGCACCCCGcc  >  2:137131/1‑151 (MQ=14)
                                                                                                  atcggcgctgccggcagccagctcagcggtcgaggcgaagccGGCTCTACCGTGCAGGTGCGTGACGTGGCAGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGc                             <  2:1774056‑M1/69‑1 (MQ=255)
                                                                                                                                                 |                                                                                          
CGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCTGGCTGAGCGGCCGGGGCGAGATCGATCAGGAAGGTGCCGTTGGCACCGACCAATCTGGTGATCGGCCTTGCCGGCAGCCAACTCAGTGGTCGTGGCGAAGTGGGCACCACCGTGCAGGTGCGTGACGCGGCCGGCAACATCCTCGCGACCGGCACCGTCGCTGCAGATGGCACCTTCAGCGTCACGCTGTTCCCCGCC  >  CP009974/1155837‑1156072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑