BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,345	A→T	V354V (GTT→GTA)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,345	0	A	T	100.0%	411.6 / NA	153	V354V (GTT→GTA)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (68/85); total (68/85)

Only 100 of 153 total aligned reads displayed.

CAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATAACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGT  >  CP009974/1554198‑1554491

 aaTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCa                                                                                                                                                <  1:1251318/151‑1 (MQ=255)

 aaTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCa                                                                                                                                                >  1:5325986/1‑151 (MQ=255)

 aaTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCa                                                                                                                                                <  2:4947505/151‑1 (MQ=255)

 aaTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCa                                                                                                                                                <  2:3118112/151‑1 (MQ=255)

 aaTCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACAGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCa                                                                                                                                                <  1:933437/151‑1 (MQ=255)

    gggTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCAttt                                                                                                                                             <  2:15773/150‑1 (MQ=255)

     ggTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTc                                                                                                                                            >  2:2781614/1‑151 (MQ=255)

      gTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCa                                                                                                                                           <  1:844133/151‑1 (MQ=255)

       tCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                          >  2:5043241/1‑151 (MQ=255)

       tCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                          <  1:2781614/151‑1 (MQ=255)

       tCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                          <  2:1829665/151‑1 (MQ=255)

       tCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                          <  2:2425615/151‑1 (MQ=255)

       tCGAGTGATTACTCGATGATTTTTGCAACAACGCCGTCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCaa                                                                                                                                          <  2:806872/151‑1 (MQ=255)

        cGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                         <  1:2343538/151‑1 (MQ=255)

        cGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAAc                                                                                                                                         <  1:5372935/151‑1 (MQ=255)

         gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                        >  1:3888608/1‑151 (MQ=255)

         gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                        >  1:4354053/1‑151 (MQ=255)

         gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                        <  1:1375318/151‑1 (MQ=255)

         gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                        >  1:1174807/1‑151 (MQ=255)

         gAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACg                                                                                                                                        <  2:1395686/151‑1 (MQ=255)

           gTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGcc                                                                                                                                      <  1:2568249/151‑1 (MQ=255)

            tGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCt                                                                                                                                     >  1:2037054/1‑151 (MQ=255)

             gATTACTCGATGATTTTTGCTACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCtt                                                                                                                                    >  2:4698922/1‑151 (MQ=255)

             gATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCtt                                                                                                                                    <  2:440236/151‑1 (MQ=255)

              aTTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTc                                                                                                                                   <  2:324241/151‑1 (MQ=255)

              aTTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTc                                                                                                                                   <  2:5193440/151‑1 (MQ=255)

               ttACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTcc                                                                                                                                  <  1:3164920/151‑1 (MQ=255)

                tACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCg                                                                                                                                 <  1:4378292/151‑1 (MQ=255)

                tACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCg                                                                                                                                 <  1:4757194/151‑1 (MQ=255)

                      atgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                           <  2:4653954/151‑1 (MQ=25)

                      atgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                           >  1:852172/1‑151 (MQ=38)

                      atgatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGtt                                                                                                                           <  2:5426313/151‑1 (MQ=25)

                       ttatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTc                                                                                                                          >  1:3431605/3‑151 (MQ=25)

                       tgatTGTTGCCACAACGCCGGCCACGCCAGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTc                                                                                                                          <  2:1336866/151‑1 (MQ=11)

                        gatTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCCGCAGTTCg                                                                                                                         >  1:1719546/1‑151 (MQ=21)

                         ctttttGCCACAACGGCGGCCCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGc                                                                                                                        <  2:5421329/150‑1 (MQ=255)

                         atTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCCTCTCAACGCCTTCCGGCAGTCCGc                                                                                                                        >  2:3752534/1‑151 (MQ=255)

                          tttttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCa                                                                                                                       >  1:460132/1‑151 (MQ=21)

                           ttttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAg                                                                                                                      <  2:1032278/151‑1 (MQ=21)

                           ttttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAg                                                                                                                      >  1:1636196/1‑151 (MQ=25)

                            tttGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGt                                                                                                                     >  1:1456709/1‑151 (MQ=25)

                              tGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTa                                                                                                                   <  1:5134254/151‑1 (MQ=18)

                              tGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTa                                                                                                                   <  2:4615154/151‑1 (MQ=18)

                                ccACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAcc                                                                                                                 <  1:3848123/151‑1 (MQ=18)

                                  acaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAc                                                                                                                  <  1:3797935/148‑1 (MQ=17)

                                  acaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTAc                                                                                                                  >  2:3797935/1‑148 (MQ=17)

                                  acaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCgg                                                                                                               >  2:3971594/1‑151 (MQ=14)

                                  acaACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCgg                                                                                                               <  2:1981746/151‑1 (MQ=14)

                                   caACTCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGt                                                                                                              >  2:4459442/1‑151 (MQ=14)

                                    aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                             >  1:3977361/1‑151 (MQ=11)

                                    aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                             <  1:121434/151‑1 (MQ=11)

                                    aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                             <  1:1357970/151‑1 (MQ=11)

                                    aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                             <  2:2623690/151‑1 (MQ=11)

                                    aaCGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTc                                                                                                             >  1:3674129/1‑151 (MQ=11)

                                                                                                         cTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttct                                        >  2:2538982/1‑151 (MQ=11)

                                                                                                         cTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttct                                        >  2:102265/1‑151 (MQ=11)

                                                                                                         cTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCcttct                                        >  2:661053/1‑151 (MQ=11)

                                                                                                           tGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCttcttc                                      <  2:3977361/151‑1 (MQ=14)

                                                                                                            ggtgaGGGCATCAGTCGTGTGGATGTTGTCACCTGGGATTAGCATTTCAACGCCTGCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                     <  2:1881656/147‑1 (MQ=11)

                                                                                                            gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                     <  1:1261264/151‑1 (MQ=14)

                                                                                                            gATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcc                                     >  1:335904/1‑151 (MQ=17)

                                                                                                             aTCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttcct                                    <  1:3975151/151‑1 (MQ=14)

                                                                                                               cAGGGTAACAGGCATCTGAATGTTGCCACCTGGCATTACCAGTACAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCGGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTcttccttc                                  <  2:4846136/151‑1 (MQ=11)

                                                                                                                aGGGTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGCACGGAGTGTGACGGCCGCCTTCTTCCTTCg                                 >  2:5291019/1‑151 (MQ=14)

                                                                                                                  ggTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGAc                               <  2:4132359/151‑1 (MQ=17)

                                                                                                                  ggTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGAc                               >  1:4765465/1‑151 (MQ=17)

                                                                                                                   gTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACa                              <  2:3674129/151‑1 (MQ=17)

                                                                                                                   gTAACAGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACa                              <  2:3673809/151‑1 (MQ=14)

                                                                                                                        aGTCATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACg                         >  2:1757113/1‑151 (MQ=17)

                                                                                                                           cATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAg                      <  2:580325/151‑1 (MQ=17)

                                                                                                                           cATCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAg                      >  1:1818638/1‑151 (MQ=17)

                                                                                                                            aTCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGa                     >  1:3674361/1‑151 (MQ=17)

                                                                                                                            aTCTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGa                     <  1:1533062/151‑1 (MQ=17)

                                                                                                                              cTGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACt                   >  1:4692263/1‑151 (MQ=17)

                                                                                                                               tGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAGCTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACtt                  <  2:1197547/151‑1 (MQ=17)

                                                                                                                               tGAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACtt                  >  2:1889153/1‑151 (MQ=17)

                                                                                                                                gAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTc                 >  1:3580440/1‑151 (MQ=17)

                                                                                                                                gAATGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTc                 <  1:2720541/151‑1 (MQ=17)

                                                                                                                                 aaTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCt                <  1:145498/151‑1 (MQ=17)

                                                                                                                                 aaTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCt                <  1:3607324/151‑1 (MQ=17)

                                                                                                                                  aTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTg               <  2:2006806/151‑1 (MQ=17)

                                                                                                                                  aTGTTGTCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTg               <  1:2049796/151‑1 (MQ=17)

                                                                                                                                    gttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCg             >  2:2991341/1‑151 (MQ=14)

                                                                                                                                    gttgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCg             <  1:1937452/151‑1 (MQ=17)

                                                                                                                                      tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGCAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGATTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACCTCTGCGGt           >  2:3352045/1‑151 (MQ=255)

                                                                                                                                      tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGt           >  1:3401760/1‑151 (MQ=17)

                                                                                                                                      tgtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCACTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGt           <  2:5162581/151‑1 (MQ=17)

                                                                                                                                       gtCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTg          >  1:1367106/1‑151 (MQ=14)

                                                                                                                                         cACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGaa        <  1:4978008/151‑1 (MQ=17)

                                                                                                                                         cACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGaa        <  2:4128790/151‑1 (MQ=17)

                                                                                                                                         cACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACCGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGaa        >  2:3237141/1‑151 (MQ=17)

                                                                                                                                          aCCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAAc       <  2:3504433/151‑1 (MQ=17)

                                                                                                                                          aCCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAAc       >  2:1822634/1‑151 (MQ=17)

                                                                                                                                           ccTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACt      >  2:5074753/1‑151 (MQ=14)

                                                                                                                                           ccTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACt      >  1:1332896/1‑151 (MQ=17)

                                                                                                                                            cTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACtt     <  1:1493523/151‑1 (MQ=17)

                                                                                                                                            cTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACtt     >  2:1403900/1‑151 (MQ=17)

                                                                                                                                             tGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTg    <  1:1798364/151‑1 (MQ=17)

                                                                                                                                              ggCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTgg   >  2:2342110/1‑151 (MQ=17)

                                                                                                                                               gCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGgt  <  2:5318244/151‑1 (MQ=17)

CAATCGGTCGAGTGATTACTCGATGATTTTTGCCACAACGCCGGCGCCGACGGTACGACCGCCTTCACGGATAGCGAAGCGCAGACCGTCTTCCATTGCGATGGTCTTGATCAGGGTAACAGTCATCTGAATGTTGTCACCTGGCATAACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGT  >  CP009974/1554198‑1554491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`