BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,630	A→G	C259C (TGT→TGC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,630	0	A	G	99.6%	507.9 / ‑5.0	223	C259C (TGT→TGC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (126/96); total (127/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 223 total aligned reads displayed.

TGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCG  >  CP009974/1554526‑1554777

tGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGAt                                                                                                       <  2:154921/151‑1 (MQ=14)

    cACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTg                                                                                                   >  2:2111736/1‑151 (MQ=17)

     aCGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGa                                                                                                  >  2:1696674/1‑151 (MQ=14)

      cGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGaa                                                                                                 >  2:4168073/1‑151 (MQ=14)

       gCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAc                                                                                                >  1:4825417/1‑151 (MQ=14)

        cTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcg                                                                                               >  2:4989360/1‑151 (MQ=17)

        cTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTCCCAGCCGATCCTCAAcc                                                                                               >  2:1013204/1‑150 (MQ=255)

         tCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcgc                                                                                              <  2:4354053/151‑1 (MQ=17)

          cAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcgcg                                                                                             >  1:4299027/1‑151 (MQ=14)

          cAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAAcgcg                                                                                             >  2:4548908/1‑151 (MQ=14)

           aaCGTCGTCGCGCTTGGTACCACGCAGCAGAACGCCGTAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCgg                                                                                            <  2:4264931/151‑1 (MQ=9)

            aCGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGa                                                                                           >  1:3116234/1‑151 (MQ=14)

             cgtcgtCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGAc                                                                                          <  2:4421336/151‑1 (MQ=14)

             cgtcgtCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGAc                                                                                          >  2:1291588/1‑151 (MQ=17)

              gtcgtcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACg                                                                                         <  1:4459442/151‑1 (MQ=17)

                cgtcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGAt                                                                                       >  2:1054222/1‑151 (MQ=14)

                  tcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAc                                                                                     >  2:1316732/1‑151 (MQ=17)

                  tcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAc                                                                                     <  1:1971812/151‑1 (MQ=14)

                  tcACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAc                                                                                     >  1:1521488/1‑151 (MQ=14)

                   cACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATAcc                                                                                    <  1:2667375/151‑1 (MQ=17)

                   cACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGAGAcc                                                                                    >  2:2731469/1‑151 (MQ=14)

                     cgcgTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCAc                                                                                  <  2:4782756/151‑1 (MQ=17)

                     cGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCAc                                                                                  >  1:2157930/1‑151 (MQ=17)

                     cGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCAc                                                                                  >  1:3517359/1‑151 (MQ=17)

                     cGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCAc                                                                                  <  1:3625109/151‑1 (MQ=17)

                       cTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATTCTGAACGCGGACGATACCACGc                                                                                <  2:4829800/151‑1 (MQ=14)

                       cTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGc                                                                                <  1:2991341/151‑1 (MQ=17)

                       cTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGc                                                                                <  1:5291019/151‑1 (MQ=17)

                        ttGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCt                                                                               <  2:390619/151‑1 (MQ=17)

                         tGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGATCGCGGACGATATCACGCtg                                                                              >  1:460437/1‑150 (MQ=9)

                         tGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTc                                                                              >  2:997324/1‑151 (MQ=14)

                                aCGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGa                                                                       >  1:3502106/1‑151 (MQ=17)

                                 cGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGAc                                                                      <  2:2037054/151‑1 (MQ=14)

                                 cGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGAc                                                                      >  1:410610/1‑151 (MQ=14)

                                 cGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGAc                                                                      >  2:3493730/1‑151 (MQ=17)

                                  gcagcaGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGAcc                                                                     >  1:2443662/1‑151 (MQ=17)

                                   cagcagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCg                                                                    <  2:1359208/151‑1 (MQ=17)

                                   cagcagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACAGCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCg                                                                    >  2:5243647/1‑151 (MQ=14)

                                     gcagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGt                                                                  >  1:1260732/1‑151 (MQ=17)

                                      cagAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTa                                                                 <  2:4044346/151‑1 (MQ=17)

                                         aaCGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTaaca                                                              >  1:148214/1‑151 (MQ=17)

                                         aaCGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTaaca                                                              >  1:4725916/1‑151 (MQ=14)

                                          aCGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTaacaa                                                             <  2:4692263/151‑1 (MQ=17)

                                            gccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACg                                                           <  1:3971594/151‑1 (MQ=17)

                                            gccgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACg                                                           >  2:3250963/1‑151 (MQ=14)

                                              cgcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGt                                                         >  2:490098/1‑151 (MQ=17)

                                               gcAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTa                                                        <  1:2138631/151‑1 (MQ=17)

                                                cAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTAc                                                       >  2:1735387/1‑151 (MQ=14)

                                                   ttCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCAc                                                    <  1:4177545/151‑1 (MQ=17)

                                                    tctcGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACg                                                   <  1:4506594/151‑1 (MQ=14)

                                                    tctcGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACg                                                   >  1:1423063/1‑151 (MQ=14)

                                                      tcGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACgac                                                 <  1:960712/151‑3 (MQ=17)

                                                       cGCCAGCACGACCTTCGGCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGacc                                                <  1:5283186/151‑1 (MQ=12)

                                                        gCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCGTGAAGGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGacca                                               >  1:1001428/1‑150 (MQ=255)

                                                         ccAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCg                                              <  2:3068919/151‑1 (MQ=17)

                                                          cAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCga                                             >  2:1077295/1‑151 (MQ=17)

                                                          cAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCga                                             >  1:5030011/1‑151 (MQ=14)

                                                          cAGCACGACCGTCGTCCATCAGCTTGCGGAACAACTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCga                                             <  2:476972/151‑1 (MQ=255)

                                                           aGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgag                                            >  2:147832/1‑151 (MQ=12)

                                                           aGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgag                                            <  2:3401760/151‑1 (MQ=9)

                                                             cACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgagac                                          >  2:656323/1‑150 (MQ=17)

                                                             cACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGAt                                          <  2:1179630/151‑1 (MQ=12)

                                                              aCGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgagagc                                         >  2:3843228/1‑149 (MQ=9)

                                                              aCGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATc                                         <  1:4926603/151‑1 (MQ=12)

                                                              aCGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATc                                         >  2:1957883/1‑151 (MQ=14)

                                                              aCGACCGTCGTCCAGCAGCTTTCTGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATc                                         <  2:787149/151‑1 (MQ=255)

                                                                gACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCga                                       <  1:1214855/151‑1 (MQ=12)

                                                                gACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCga                                       >  1:708237/1‑151 (MQ=17)

                                                                   cTTCGTCCAGCAGCTTGCGGAACAGCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaa                                    <  2:376350/151‑1 (MQ=12)

                                                                    ttCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaac                                   >  1:1896086/1‑150 (MQ=12)

                                                                    ttCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaac                                   >  1:800549/1‑150 (MQ=12)

                                                                     tCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGaaca                                  >  2:700176/1‑149 (MQ=12)

                                                                      cGTCCAGCCGCTGGCGGAATATCTGGACGCCGGTGCAGGGGGTGGTGGTGGTGTCACGCCGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGTTACTACCGTTAACAACGGTACCACGACCCGAGATCGAGAacac                                 <  2:5283461/151‑1 (MQ=11)

                                                                       gTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACg                                                   <  2:2599758/132‑1 (MQ=17)

                                                                       gTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACg                                                   >  1:2599758/1‑132 (MQ=17)

                                                                       gTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACg                                <  1:5430221/151‑1 (MQ=12)

                                                                         ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTCCCACGACCCGAGATCGAGAACACGTc                              >  2:4645146/1‑151 (MQ=255)

                                                                         ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTc                              <  2:3647442/151‑1 (MQ=12)

                                                                         ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTc                              >  1:5365636/1‑151 (MQ=255)

                                                                          cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCt                             <  1:5074753/151‑1 (MQ=255)

                                                                          cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCt                             <  2:1760097/151‑1 (MQ=12)

                                                                           agcagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCtt                            >  2:2904827/1‑151 (MQ=9)

                                                                             cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                          >  1:756295/1‑151 (MQ=12)

                                                                              agcTTGCGGAACATCTCAACACCGGTGCAGGGGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCgg                         >  1:2759012/1‑150 (MQ=255)

                                                                                 ttGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTg                      >  1:2056757/1‑151 (MQ=255)

                                                                                 gtGCGGAAGATCGGAACACGGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACGAAGGATTTCCAGCGGATCCTGAAGGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCGCGATCCGAGATCGAGAACACGTCTTCGATTg                      <  2:3130555/150‑1 (MQ=11)

                                                                                  tGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTgg                     >  2:4509430/1‑151 (MQ=12)

                                                                                    cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                   >  2:2478206/1‑151 (MQ=9)

                                                                                    cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                   >  2:3209495/1‑151 (MQ=12)

                                                                                      gAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATc                 <  1:4509474/151‑1 (MQ=12)

                                                                                        aCATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAg               >  2:2412671/1‑151 (MQ=255)

                                                                                        aCATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAg               >  1:103533/1‑151 (MQ=12)

                                                                                         cATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAgg              >  1:3263294/1‑151 (MQ=255)

                                                                                         cATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAgg              <  1:650103/151‑1 (MQ=9)

                                                                                          aTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGa             >  1:1527607/1‑151 (MQ=255)

                                                                                          aTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGa             >  1:2865692/1‑151 (MQ=255)

                                                                                            ctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCCATTGGCATCAGGAAc           >  2:3587451/1‑151 (MQ=255)

                                                                                              cagcgccGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACgg         <  1:3352045/146‑1 (MQ=11)

                                                                                                   ccGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGt    >  1:1782115/1‑151 (MQ=12)

                                                                                                     ggTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCg  >  1:4936950/1‑151 (MQ=12)

TGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCG  >  CP009974/1554526‑1554777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`