BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,729	C→A	G226G (GGG→GGT)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,729	0	C	A	99.0%	835.6 / NA	290	G226G (GGG→GGT)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base C (2/1); new base A (161/126); total (163/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGC  >  CP009974/1554581‑1554875

 gCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCGTGAAGGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGacca                                                                                                                                                 >  1:1001428/1‑150 (MQ=255)

  ccAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCg                                                                                                                                                >  2:613599/1‑151 (MQ=12)

  ccAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCg                                                                                                                                                <  2:3068919/151‑1 (MQ=17)

   cAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCga                                                                                                                                               >  2:1077295/1‑151 (MQ=17)

    aGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgag                                                                                                                                              >  2:147832/1‑151 (MQ=12)

     gCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCgaga                                                                                                                                             >  2:2024041/1‑151 (MQ=12)

      cACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGAt                                                                                                                                            <  2:1179630/151‑1 (MQ=12)

       aCGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATc                                                                                                                                           <  2:1216780/151‑1 (MQ=17)

         gACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCga                                                                                                                                         <  1:1214855/151‑1 (MQ=12)

         gACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCga                                                                                                                                         >  1:3747832/1‑151 (MQ=9)

          aCCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTAGTGTAACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCCCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCgag                                                                                                                                        <  2:69618/151‑1 (MQ=11)

           ccTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCgaga                                                                                                                                       >  1:3282003/1‑151 (MQ=14)

               cGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAacac                                                                                                                                   >  2:4432120/1‑151 (MQ=12)

               cGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAacac                                                                                                                                   >  2:2673293/1‑151 (MQ=9)

                  ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTc                                                                                                                                <  2:3647442/151‑1 (MQ=12)

                      cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                            >  1:756295/1‑151 (MQ=12)

                       agcTTGCGGAACATCTCAACACCGGTGCAGGGGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCgg                                                                                                                           >  1:2759012/1‑150 (MQ=255)

                         cTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAtt                                                                                                                         <  1:4459219/151‑1 (MQ=255)

                         cTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAtt                                                                                                                         <  2:4375736/151‑1 (MQ=9)

                          ttGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTg                                                                                                                        <  1:102265/151‑1 (MQ=255)

                             cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                                                                                                                     >  2:2478206/1‑151 (MQ=9)

                             cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                                                                                                                     >  2:3209495/1‑151 (MQ=12)

                               gAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATc                                                                                                                   <  1:5231819/151‑1 (MQ=9)

                                   aTCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGa                                                                                                               >  2:1017731/1‑151 (MQ=12)

                                     ctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCCATTGGCATCAGGAAc                                                                                                             >  2:3587451/1‑151 (MQ=255)

                                      tcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACg                                                                                                            >  1:898145/1‑151 (MQ=9)

                                       cagcgccGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACgg                                                                                                           <  1:3352045/146‑1 (MQ=11)

                                           acCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTgg                                                                                                       >  1:5012367/1‑151 (MQ=255)

                                            ccggggcAGGTGGTGGTGGTGGCGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCCCGACCCGCGATCGAGAACACGTCCTCGATGGGCATCAGGAACGGCTggg                                                                                                      >  2:3755421/7‑150 (MQ=11)

                                            ccGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGt                                                                                                      >  1:1782115/1‑151 (MQ=12)

                                             cGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTc                                                                                                     <  2:3827381/151‑1 (MQ=12)

                                               gtgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGa                                                                                                   >  1:544475/4‑151 (MQ=9)

                                                 gcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGGCTTCCATTGGCATCAGGAACGGCCGGTCGATg                                                                                                 >  1:4101209/2‑151 (MQ=11)

                                                   aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                               >  2:524268/1‑151 (MQ=17)

                                                   aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTCCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                               >  2:3854692/1‑151 (MQ=9)

                                                    ggtggtggtggtggtGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCa                                                                                              <  1:3425067/151‑1 (MQ=14)

                                                     gtggtggtggtggtgTCACGCAGACCAACGCTTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCAc                                                                                             <  1:4953533/151‑1 (MQ=9)

                                                           gtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTg                                                                                       <  1:4525674/151‑1 (MQ=14)

                                                             ggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGTAACTGCTGGTCGATGGCACGACCTGGc                                                                                     >  1:134186/1‑151 (MQ=255)

                                                             ggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGc                                                                                     <  1:4810526/151‑1 (MQ=14)

                                                             ggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGc                                                                                     >  1:4494830/1‑151 (MQ=12)

                                                                ggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCa                                                                                  <  2:3870583/151‑1 (MQ=9)

                                                                 gtgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGCTGGCACGAACCGGCTCAg                                                                                 >  2:4139274/1‑151 (MQ=255)

                                                                 gtgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAg                                                                                 <  1:1822634/151‑1 (MQ=12)

                                                                 gtgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAg                                                                                 <  2:5262793/151‑1 (MQ=14)

                                                                  tgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAgg                                                                                <  1:1659410/151‑1 (MQ=14)

                                                                  tgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAgg                                                                                >  1:3136571/1‑151 (MQ=17)

                                                                    tCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGa                                                                              <  1:1969567/151‑1 (MQ=16)

                                                                     cACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAt                                                                             <  2:4618291/151‑1 (MQ=16)

                                                                        gCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCt                                                                                    <  1:1466117/141‑1 (MQ=17)

                                                                        gCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCt                                                                                    >  2:1466117/1‑141 (MQ=17)

                                                                         gagcccAACGATTTCCAGCGGATCCTGACCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAg                                                                         <  2:573083/147‑1 (MQ=255)

                                                                              cgtcGATTTGCAGCGGATGCTGAACGCGGACGGGACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGGGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATc                                                                    <  1:5400637/148‑1 (MQ=11)

                                                                              cAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATc                                                                    >  2:1710789/1‑151 (MQ=255)

                                                                                  gATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCaga                                                                <  2:4765465/151‑1 (MQ=255)

                                                                                  gATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCaga                                                                <  1:268193/151‑1 (MQ=255)

                                                                                    tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTATTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                              <  2:3449043/151‑1 (MQ=255)

                                                                                     ttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGtt                                                             <  2:229923/151‑1 (MQ=255)

                                                                                        cAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCt                                                          >  2:2188368/1‑151 (MQ=255)

                                                                                         aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTCCCAAGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                         <  1:4909042/151‑1 (MQ=255)

                                                                                         aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                         >  1:507566/1‑151 (MQ=255)

                                                                                            ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                      <  2:3364364/151‑1 (MQ=255)

                                                                                            ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                      <  1:4168073/151‑1 (MQ=255)

                                                                                            ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                      >  1:5336437/1‑151 (MQ=255)

                                                                                                 cTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttc                                                 >  2:4909930/1‑151 (MQ=255)

                                                                                                  tGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttct                                                <  2:2792880/151‑1 (MQ=255)

                                                                                                   gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                               >  1:2412360/1‑151 (MQ=255)

                                                                                                   gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                               >  1:842954/1‑151 (MQ=255)

                                                                                                    aaCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTg                                              <  2:4309420/151‑1 (MQ=255)

                                                                                                     aCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGa                                             >  1:3600028/1‑151 (MQ=255)

                                                                                                       gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                           <  1:1054222/151‑1 (MQ=255)

                                                                                                       gcgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACa                                           <  2:1772474/151‑1 (MQ=255)

                                                                                                        cgGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAg                                          >  1:47396/1‑151 (MQ=255)

                                                                                                         ggACGATACCACGCTCGATACGACCGGTAACAACGGTGCCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGc                                         >  1:2178343/1‑151 (MQ=255)

                                                                                                          gACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCg                                        <  1:3040081/151‑1 (MQ=255)

                                                                                                            cGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGt                                      >  2:4176868/1‑151 (MQ=255)

                                                                                                             gATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAAGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTa                                     >  2:870950/1‑151 (MQ=255)

                                                                                                             gATACCACGCACGATACGAACGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTa                                     <  2:4232444/151‑1 (MQ=255)

                                                                                                              aTACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAg                                    >  1:2954883/1‑151 (MQ=255)

                                                                                                               tACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGt                                   <  2:1343602/151‑1 (MQ=255)

                                                                                                                   aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                               >  2:3915004/1‑151 (MQ=255)

                                                                                                                   aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                               <  2:2443662/151‑1 (MQ=255)

                                                                                                                    cGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGGCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTGTCTACCAGCTTCTTGACAGCGTTAGTGCCCa                              >  2:4245836/1‑151 (MQ=14)

                                                                                                                       tCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAttt                           <  2:5365636/151‑1 (MQ=255)

                                                                                                                          aTACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGt                        >  2:3412104/1‑151 (MQ=255)

                                                                                                                           tACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGtt                       <  1:705313/151‑1 (MQ=255)

                                                                                                                            aCGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTg                      >  2:5026844/1‑151 (MQ=255)

                                                                                                                            aCGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTg                      <  1:622870/151‑1 (MQ=255)

                                                                                                                            aCGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTg                      >  1:4648746/1‑151 (MQ=255)

                                                                                                                              gACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtc                    <  2:467699/151‑1 (MQ=255)

                                                                                                                              gACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtc                    >  1:3231989/1‑151 (MQ=255)

                                                                                                                                ccGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcgt                  >  2:3156019/1‑151 (MQ=255)

                                                                                                                                  ggTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCt                <  1:2124787/151‑1 (MQ=255)

                                                                                                                                     aacaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTTTCGTCTTTg             >  2:409143/1‑151 (MQ=255)

                                                                                                                                      acaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGc            >  2:1587439/1‑151 (MQ=255)

                                                                                                                                        aacGGTACCACAACCCGAGATCGAGAACACGTCTTCGATTGGCATCGGGAACGGCTGGTCGATGGGTCGAACTGGCTCAGGGAGGTAGCCATCCAGAGCTTCTACCAGCTTCTTGACAGCGGGAGTGCCCATCTCTTTGTCTTCTTTGCCt          >  2:4970892/1‑151 (MQ=11)

                                                                                                                                          cGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTc        >  2:2876005/1‑151 (MQ=255)

                                                                                                                                             tACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAg     <  1:3868974/151‑1 (MQ=255)

                                                                                                                                              aCCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCTCATTTCGTTGTCGTCTTTGCCTTCCAgg    >  2:1296283/1‑151 (MQ=255)

                                                                                                                                                cACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTCGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGc  >  2:791523/1‑151 (MQ=255)

CGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGC  >  CP009974/1554581‑1554875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`