BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,554,744	T→C	V221V (GTA→GTG)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,554,744	0	T	C	99.7%	873.7 / NA	289	V221V (GTA→GTG)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (152/136); total (153/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

CGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACC  >  CP009974/1554596‑1554890

 gTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACg                                                                                                                                                 <  1:5430221/151‑1 (MQ=12)

   ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTc                                                                                                                                               <  2:3647442/151‑1 (MQ=12)

   ccAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTc                                                                                                                                               >  1:5365636/1‑151 (MQ=255)

    cagcagCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCt                                                                                                                                              <  2:1760097/151‑1 (MQ=12)

     agcagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCtt                                                                                                                                             >  2:2904827/1‑151 (MQ=9)

       cagcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCg                                                                                                                                           >  1:756295/1‑151 (MQ=12)

        agcTTGCGGAACATCTCAACACCGGTGCAGGGGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCgg                                                                                                                                          >  1:2759012/1‑150 (MQ=255)

         gcTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGAt                                                                                                                                         >  2:4350241/1‑151 (MQ=12)

           ttGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTg                                                                                                                                       >  1:2056757/1‑151 (MQ=255)

              cGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCa                                                                                                                                    >  2:3209495/1‑151 (MQ=12)

                gAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATc                                                                                                                                  <  1:4509474/151‑1 (MQ=12)

                gAACATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATc                                                                                                                                  <  1:5231819/151‑1 (MQ=9)

                  aCATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAg                                                                                                                                >  1:103533/1‑151 (MQ=12)

                  aCATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAg                                                                                                                                >  2:2412671/1‑151 (MQ=255)

                   cagctcCACACCGGTGCAGGTGGTGGTGGTGGGGTGACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAgg                                                                                                                               <  2:497746/148‑1 (MQ=11)

                   cATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAgg                                                                                                                               <  1:650103/151‑1 (MQ=9)

                   cATCTCAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAgg                                                                                                                               <  2:2297065/151‑1 (MQ=255)

                      ctcAACACCGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCCATTGGCATCAGGAAc                                                                                                                            >  2:3587451/1‑151 (MQ=255)

                        cagcgccGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACgg                                                                                                                          <  1:3352045/146‑1 (MQ=11)

                             ccggggcAGGTGGTGGTGGTGGCGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCCCGACCCGCGATCGAGAACACGTCCTCGATGGGCATCAGGAACGGCTggg                                                                                                                     >  2:3755421/7‑150 (MQ=11)

                              cGGTGCAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTc                                                                                                                    <  2:3827381/151‑1 (MQ=12)

                                gtgcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGa                                                                                                                  >  1:4105270/4‑151 (MQ=12)

                                  gcAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATg                                                                                                                <  1:4317694/150‑1 (MQ=12)

                                    aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                                              >  1:1058190/1‑151 (MQ=17)

                                    aGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTCCGATTGGCATCAGGAACGGCTGGTCGATGGc                                                                                                              >  2:3854692/1‑151 (MQ=9)

                                      gtggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCAc                                                                                                            <  1:178897/151‑1 (MQ=12)

                                       tggtggtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACg                                                                                                           >  2:4138760/1‑151 (MQ=14)

                                            gtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTg                                                                                                      <  2:508425/151‑1 (MQ=12)

                                            gtggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTg                                                                                                      >  1:2547282/1‑151 (MQ=12)

                                              ggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGc                                                                                                    >  1:4494788/1‑151 (MQ=12)

                                              ggtggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGc                                                                                                    <  1:4810526/151‑1 (MQ=14)

                                                 ggtgTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCa                                                                                                 <  2:3870583/151‑1 (MQ=9)

                                                   tgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAgg                                                                                               <  1:1659410/151‑1 (MQ=14)

                                                   tgtCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAgg                                                                                               >  1:3136571/1‑151 (MQ=17)

                                                      cACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAt                                                                                            <  2:4618291/151‑1 (MQ=16)

                                                      cACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAt                                                                                            <  1:613599/151‑1 (MQ=16)

                                                         gCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTa                                                                                         >  2:2832632/1‑151 (MQ=25)

                                                                   gATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCaga                                                                               <  2:4765465/151‑1 (MQ=255)

                                                                   gATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCaga                                                                               <  1:3752534/151‑1 (MQ=255)

                                                                     tttCGAGCGAATCCTGAACGCGGACGATACTCCGCTCGATACGACCGGTAAAAACGGTACCACGACCGGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                                             <  2:539167/151‑1 (MQ=14)

                                                                     tttCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTATTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                                             <  2:3449243/151‑1 (MQ=255)

                                                                     tttCCAGCGCATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGACCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGt                                                                             <  2:4242896/151‑1 (MQ=255)

                                                                          aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                                        >  1:507566/1‑151 (MQ=255)

                                                                          aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                                        >  2:4394973/1‑151 (MQ=255)

                                                                          aGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTa                                                                        >  2:1178057/1‑151 (MQ=255)

                                                                           gCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTAc                                                                       <  1:1406791/151‑1 (MQ=255)

                                                                             ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                                     <  2:3364364/151‑1 (MQ=255)

                                                                             ggATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCa                                                                     >  1:905348/1‑151 (MQ=255)

                                                                                 ccTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGctt                                                                 <  1:4666110/151‑1 (MQ=255)

                                                                                    gAACGTGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                                              <  1:3946092/151‑1 (MQ=255)

                                                                                    gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                                              >  1:844142/1‑151 (MQ=255)

                                                                                    gAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGcttctt                                                              >  1:3525420/1‑151 (MQ=255)

                                                                                     aaCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTg                                                             <  1:2362114/151‑1 (MQ=255)

                                                                                      aCGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGa                                                            >  1:3600028/1‑151 (MQ=255)

                                                                                          ggACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGCCTGGTCGACGGCACGAACTAGCTCAGGGATGTAAGCATCCAGAGTTTCTACCAGCTCCTTgccccc                                                        >  2:2459390/1‑146 (MQ=14)

                                                                                           gACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCg                                                       <  1:3040081/151‑1 (MQ=255)

                                                                                            aCGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCgg                                                      >  2:5299117/1‑151 (MQ=255)

                                                                                             cGATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGt                                                     <  1:2125438/151‑1 (MQ=255)

                                                                                              gATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTa                                                    >  2:3421956/1‑151 (MQ=255)

                                                                                              gATACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGAAGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTa                                                    >  2:870950/1‑151 (MQ=255)

                                                                                               aTACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAg                                                   >  1:2954883/1‑151 (MQ=255)

                                                                                               aTACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGCCGATGGCACGAACTGGCCCAGGGCTGTAGGCATCCAGAGGTTCAACCAGCTTCTTGGCAACGGTAg                                                   >  2:593637/1‑151 (MQ=14)

                                                                                                tACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGt                                                  <  1:1006469/151‑1 (MQ=255)

                                                                                                tACCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGt                                                  <  2:1343602/151‑1 (MQ=255)

                                                                                                  ccACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGc                                                <  2:5245779/151‑1 (MQ=255)

                                                                                                    aCGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGccc                                              >  2:3915004/1‑151 (MQ=255)

                                                                                                      gCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAt                                            <  1:3282105/151‑1 (MQ=255)

                                                                                                      gCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCAt                                            <  1:1291588/151‑1 (MQ=255)

                                                                                                          gATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCg                                        >  2:4937217/1‑151 (MQ=255)

                                                                                                               gACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtc                                   <  2:467699/151‑1 (MQ=255)

                                                                                                               gACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtc                                   >  1:3231989/1‑151 (MQ=255)

                                                                                                                aCCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcg                                  <  1:4959326/151‑1 (MQ=255)

                                                                                                                 ccGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTgtcgt                                 >  2:3156019/1‑151 (MQ=255)

                                                                                                                      aacaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTTTCGTCTTTg                            >  2:409143/1‑151 (MQ=255)

                                                                                                                       acaacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGc                           >  2:1587439/1‑151 (MQ=255)

                                                                                                                        caacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGcc                          <  2:4291296/151‑1 (MQ=255)

                                                                                                                        caacGGTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCCTTGcc                          >  2:2580729/1‑151 (MQ=255)

                                                                                                                            ggTACCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTcc                      >  2:1476828/1‑151 (MQ=255)

                                                                                                                               aCCACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCTCATTTCGTTGTCGTCTTTGCCTTCCAgg                   >  2:1296283/1‑151 (MQ=255)

                                                                                                                                 cACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGc                 >  2:1066904/1‑151 (MQ=255)

                                                                                                                                 cACGACCCGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGc                 >  1:2690772/1‑151 (MQ=255)

                                                                                                                                   cgacccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCa               >  1:4644853/4‑151 (MQ=255)

                                                                                                                                     acccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATa             <  1:1839080/150‑1 (MQ=255)

                                                                                                                                      cccGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATAc            <  2:2684953/151‑1 (MQ=255)

                                                                                                                                        cGAGATCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGa          >  1:718844/1‑151 (MQ=255)

                                                                                                                                         gagaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAg         <  1:490098/151‑1 (MQ=255)

                                                                                                                                         gagaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAg         >  2:4481425/1‑151 (MQ=255)

                                                                                                                                           gaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCg       <  1:175455/151‑1 (MQ=255)

                                                                                                                                           gaTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCg       >  1:1482773/1‑151 (MQ=255)

                                                                                                                                           gaTCGAGAACACCTCATAGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAAGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCCTACGAGCg       <  2:167857/151‑1 (MQ=14)

                                                                                                                                            aTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCgg      <  1:4432120/151‑1 (MQ=255)

                                                                                                                                            aTCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCgg      >  1:4952142/1‑151 (MQ=255)

                                                                                                                                             tCGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGa     <  1:2673293/151‑1 (MQ=255)

                                                                                                                                              cGAGAACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGCTGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGaa    <  2:4149771/151‑1 (MQ=255)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTCGTGCCCATTTCGTTGTCGTCTTTTCCTTCCAGGGCCATACGAGCGGCAc   >  2:4229128/1‑151 (MQ=255)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc   <  2:2157930/151‑1 (MQ=255)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc   <  2:1260732/151‑1 (MQ=255)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc   <  2:756295/151‑1 (MQ=255)

                                                                                                                                               gagaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAc   <  2:2012641/151‑1 (MQ=38)

                                                                                                                                                agaACACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAAcc  >  2:1459624/1‑151 (MQ=255)

CGTCCAGCAGCTTGCGGAACATCTCAACACCGGTACAGGTGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGGACGATACCACGCTCGATACGACCGGTAACAACGGTACCACGCCCCGAGATCGAGAATACGTCTTCGATTGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACC  >  CP009974/1554596‑1554890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`