BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,555,277	T→C	I44V (ATC→GTC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,555,277	0	T	C	99.6%	590.7 / NA	258	I44V (ATC→GTC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (131/126); minor base G (0/1); total (131/127)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

ATCATGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGC  >  CP009974/1555129‑1555423

 tCATGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGc                                                                                                                                                 <  1:4813662/151‑1 (MQ=25)

       tcttcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaa                                                                                                                                             <  1:2088052/149‑1 (MQ=17)

        cttcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaaccg                                                                                                                                          >  1:4580766/1‑151 (MQ=17)

          tcACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGccgaaccgaa                                                                                                                                        <  1:2763665/151‑1 (MQ=17)

           cACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGaaa                                                                                                                                       >  1:4360982/1‑151 (MQ=17)

            aCGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGaaaa                                                                                                                                      <  1:4292687/151‑1 (MQ=17)

              gTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACt                                                                                                                                    <  2:2933193/151‑1 (MQ=14)

                   cAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGa                                                                                                                               >  2:1414581/1‑151 (MQ=17)

                   cAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGa                                                                                                                               >  2:5016546/1‑151 (MQ=17)

                   cAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAACGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCggg                                                                                                                               >  1:1185608/1‑150 (MQ=17)

                      cGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATCCCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCa                                                                                                                            <  1:765553/151‑1 (MQ=14)

                       gTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCag                                                                                                                           <  2:4063074/151‑2 (MQ=14)

                        tGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCaga                                                                                                                          <  1:1077295/151‑3 (MQ=17)

                         gACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGCGTTGTACTCGACGTGAGCGGTGTGGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGCGCAGAc                                                                                                                         >  1:1809262/1‑151 (MQ=255)

                         gACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAc                                                                                                                         <  1:749974/151‑1 (MQ=17)

                             tGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGa                                                                                                                     <  1:2170384/151‑1 (MQ=12)

                                gCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTc                                                                                                                  <  1:173318/151‑1 (MQ=14)

                                 cAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCa                                                                                                                 >  1:4479344/1‑151 (MQ=12)

                                  aGTCAACGTCAGCGTAGTGACGAATGTTCGACTTGTATTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGGTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCag                                                                                                                <  2:4256298/151‑1 (MQ=11)

                                     cAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGc                                                                                                             >  2:378462/1‑151 (MQ=12)

                                       aCGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTg                                                                                                           >  1:5413952/1‑151 (MQ=12)

                                        cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGc                                                                                                          <  1:3586221/151‑1 (MQ=12)

                                         gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                         >  2:4540396/1‑151 (MQ=12)

                                          tGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCgg                                                                                                        <  1:3135577/151‑1 (MQ=12)

                                          ggAGCGTAGTGACGAATGTTCGAGTTGGACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCGTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCgg                                                                                                        <  1:3687130/150‑1 (MQ=255)

                                           gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGt                                                                                                       >  2:3900379/1‑151 (MQ=17)

                                           gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGt                                                                                                       <  2:4928831/151‑1 (MQ=9)

                                            ggCGGAGTGACGAATGTTCGAGTTGTACTCGAGGTGGGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                      <  2:3002209/150‑1 (MQ=11)

                                            aGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                      <  2:4108876/151‑1 (MQ=12)

                                             gCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCa                                                                                                     <  2:542267/151‑1 (MQ=9)

                                               gTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCaga                                                                                                   >  1:4732777/1‑151 (MQ=12)

                                               gTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCaga                                                                                                   <  1:3757291/151‑1 (MQ=9)

                                                tAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCagag                                                                                                  >  1:247904/1‑151 (MQ=17)

                                                 aGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGt                                                                                                 <  1:2403163/151‑1 (MQ=9)

                                                 aGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGt                                                                                                 >  2:2252079/1‑151 (MQ=17)

                                                  gTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTg                                                                                                >  1:1750635/1‑151 (MQ=12)

                                                   tGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACCTCGGAGCAGCCGCGAGTCAGAGCTGCGGTCagacggg                                                                                               >  2:3114385/1‑147 (MQ=11)

                                                      cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                            <  1:5315820/151‑1 (MQ=12)

                                                       gAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCtt                                                                                           >  1:3508653/1‑151 (MQ=12)

                                                        aaTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTa                                                                                          <  1:93935/151‑1 (MQ=12)

                                                        aaTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTa                                                                                          >  2:3751496/1‑151 (MQ=9)

                                                         aTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTAc                                                                                         <  1:4026383/151‑1 (MQ=9)

                                                         aTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTAc                                                                                         >  2:2209452/1‑151 (MQ=9)

                                                           gTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCg                                                                                       <  2:2313741/151‑1 (MQ=9)

                                                                              tGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                    <  1:325983/151‑1 (MQ=14)

                                                                              tGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                    <  2:3429222/151‑1 (MQ=9)

                                                                               gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCTATCTTGTCGAACTCTACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                   <  2:5201461/151‑1 (MQ=255)

                                                                               gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                   <  2:3866984/151‑1 (MQ=14)

                                                                               gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                   <  1:4229128/151‑1 (MQ=9)

                                                                               gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                   >  2:5288201/1‑151 (MQ=14)

                                                                               gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                   <  2:4322402/151‑1 (MQ=14)

                                                                                aGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                  <  1:4966541/151‑1 (MQ=9)

                                                                                aGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                  <  2:716775/151‑1 (MQ=9)

                                                                                 gCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGc                                                                 <  1:1650648/151‑1 (MQ=9)

                                                                                  cGGTGTTGATGGTGATACCGCGCGCTTTTTCTGCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGcc                                                                <  2:4815662/151‑1 (MQ=12)

                                                                                   ggTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCg                                                               >  2:3146649/1‑151 (MQ=14)

                                                                                    gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                              <  1:2526574/151‑1 (MQ=12)

                                                                                    gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                              <  2:640282/151‑1 (MQ=12)

                                                                                    gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                              >  1:3343891/1‑151 (MQ=12)

                                                                                    gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                              <  1:3975175/151‑1 (MQ=12)

                                                                                    gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGACCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                              <  2:3362483/151‑1 (MQ=12)

                                                                                     tgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGAc                                                             <  2:2520413/151‑1 (MQ=12)

                                                                                        tGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGtt                                                          <  2:178166/151‑1 (MQ=17)

                                                                                        tGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGtt                                                          <  2:1993373/151‑1 (MQ=12)

                                                                                           tGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAAc                                                       <  2:2716113/151‑1 (MQ=12)

                                                                                            ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGCCGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACg                                                      <  1:2060648/151‑1 (MQ=9)

                                                                                              tGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTg                                                    <  2:1127140/151‑1 (MQ=12)

                                                                                                 tACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTgggg                                                 >  1:3223826/1‑151 (MQ=9)

                                                                                                  aCCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGggga                                                >  2:2451314/1‑151 (MQ=12)

                                                                                                    cgcgcgCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCCAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCCGTCAGCGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTCATCGTggcggag                                              >  2:1557464/1‑146 (MQ=11)

                                                                                                     gcgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagg                                             <  1:4656645/151‑1 (MQ=12)

                                                                                                     gcgcgcTGTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagg                                             <  2:4558286/151‑1 (MQ=12)

                                                                                                        cgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagggaa                                          >  1:590239/1‑151 (MQ=9)

                                                                                                         gcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAAc                                         >  1:1467977/1‑151 (MQ=12)

                                                                                                              ttcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCa                                    >  1:3436557/1‑151 (MQ=12)

                                                                                                               tcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaa                                   <  1:205086/151‑1 (MQ=17)

                                                                                                                 ttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAt                                 >  1:2349868/1‑151 (MQ=12)

                                                                                                                    cGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTAACGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAtttt                              <  1:1239545/151‑1 (MQ=255)

                                                                                                                     ggAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAttttt                             >  1:2495610/1‑151 (MQ=16)

                                                                                                                      gAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTc                            <  1:1848279/151‑1 (MQ=18)

                                                                                                                          cGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTa                        <  1:3163303/151‑1 (MQ=255)

                                                                                                                            aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAATGATCAAATTTTTCTTTAGc                      >  1:409411/1‑151 (MQ=255)

                                                                                                                            aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGc                      >  2:2500578/1‑151 (MQ=255)

                                                                                                                             gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                     <  1:3049262/151‑1 (MQ=255)

                                                                                                                             gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                     >  2:4960399/1‑151 (MQ=255)

                                                                                                                             gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                     <  2:3422715/151‑1 (MQ=255)

                                                                                                                              tCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCa                    <  2:4637518/151‑1 (MQ=255)

                                                                                                                               cGATCTTGTCGAACTCAACGACTGCCGGACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCAc                   <  2:1815795/151‑1 (MQ=255)

                                                                                                                               cGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCAc                   <  2:4145195/151‑1 (MQ=255)

                                                                                                                                 aTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGa                 >  1:1910175/1‑151 (MQ=255)

                                                                                                                                   cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa               <  1:5160120/151‑1 (MQ=255)

                                                                                                                                   cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa               <  1:1339756/151‑1 (MQ=255)

                                                                                                                                     tGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGt             <  2:1192295/151‑1 (MQ=255)

                                                                                                                                      gTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTg            <  2:2633601/151‑1 (MQ=255)

                                                                                                                                        cGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGaa          >  2:1969303/1‑151 (MQ=255)

                                                                                                                                         gAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAAc         >  1:1030666/1‑151 (MQ=255)

                                                                                                                                           aCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACct       >  1:3917937/1‑151 (MQ=255)

                                                                                                                                              cAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGGGAACCTCtt    >  2:4494050/1‑151 (MQ=255)

                                                                                                                                               aaCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTg   >  1:4898233/1‑151 (MQ=255)

                                                                                                                                                aCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGc  >  1:2342142/1‑151 (MQ=255)

ATCATGTTCTTCACGTAGTCAGCGTGACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGC  >  CP009974/1555129‑1555423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`