BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,555,302	A→G	V35V (GTT→GTC)	tuf ←	elongation factor Tu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,555,302	0	A	G	99.6%	679.4 / NA	264	V35V (GTT→GTC)	tuf	elongation factor Tu
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (125/138); minor base C (1/0); total (126/138)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

GACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGTTTTTTA  >  CP009974/1555154‑1555449

gACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGCTCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAc                                                                                                                                                   >  2:265476/1‑151 (MQ=9)

gACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAc                                                                                                                                                   <  1:749974/151‑1 (MQ=17)

  ccTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGAcgc                                                                                                                                                 >  1:4310614/1‑151 (MQ=14)

    tGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGa                                                                                                                                               <  1:2170384/151‑1 (MQ=12)

      ggCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCACACGCGAgg                                                                                                                                             >  2:3848354/1‑150 (MQ=12)

       gCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTc                                                                                                                                            <  1:173318/151‑1 (MQ=14)

        cAGTCAACGTGAGCGTAGTGACGAATGTTCGGGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCa                                                                                                                                           <  2:706817/151‑1 (MQ=9)

           tCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCagag                                                                                                                                        >  1:1870275/1‑151 (MQ=17)

               cGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTAAGAGCTGc                                                                                                                                    >  1:2484618/1‑151 (MQ=9)

                gTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCg                                                                                                                                   >  2:4540396/1‑151 (MQ=12)

                 ggAGCGTAGTGACGAATGTTCGAGTTGGACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCGTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCgg                                                                                                                                  <  1:3687130/150‑1 (MQ=255)

                  gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGt                                                                                                                                 <  2:4928831/151‑1 (MQ=9)

                  gAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGACCTGCGGt                                                                                                                                 >  1:182625/1‑151 (MQ=12)

                   ggCGGAGTGACGAATGTTCGAGTTGTACTCGAGGTGGGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                                                <  2:3002209/150‑1 (MQ=11)

                   aGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                                                <  1:2060151/151‑1 (MQ=12)

                   aGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTc                                                                                                                                >  2:4389984/1‑151 (MQ=12)

                      gTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCaga                                                                                                                             >  1:4732777/1‑151 (MQ=12)

                      gTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCaga                                                                                                                             <  1:3757291/151‑1 (MQ=9)

                        aGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCagagg                                                                                                                           >  1:4811388/1‑150 (MQ=12)

                         gTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTg                                                                                                                          >  1:1924172/1‑151 (MQ=12)

                         gTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTg                                                                                                                          <  2:376918/151‑1 (MQ=14)

                          tGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACCTCGGAGCAGCCGCGAGTCAGAGCTGCGGTCagacggg                                                                                                                         >  2:3114385/1‑147 (MQ=11)

                             cGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCt                                                                                                                      <  1:5315820/151‑1 (MQ=12)

                              gAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCtt                                                                                                                     >  1:3508653/1‑151 (MQ=12)

                              gAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCtt                                                                                                                     >  1:24160/1‑151 (MQ=12)

                               aaTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTa                                                                                                                    >  2:1000658/1‑151 (MQ=9)

                                aTGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGCCAGAGCTGCGGGCAGAGTGGTCTTAc                                                                                                                   >  1:3280023/1‑151 (MQ=255)

                                  gTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCg                                                                                                                 <  2:2313741/151‑1 (MQ=9)

                                   ttCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGt                                                                                                                <  2:5306597/151‑1 (MQ=2)

                                   ttCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGt                                                                                                                >  1:2309026/1‑151 (MQ=11)

                                                     tGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                                              <  1:325983/151‑1 (MQ=14)

                                                     tGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATgg                                                                                              <  2:3429222/151‑1 (MQ=9)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCTATCTTGTCGAACTCTACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                             <  2:5201461/151‑1 (MQ=255)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                             <  2:1201539/151‑1 (MQ=9)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                             <  2:4322402/151‑1 (MQ=14)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                             <  1:5399138/151‑1 (MQ=9)

                                                      gAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGt                                                                                             <  1:4229128/151‑1 (MQ=9)

                                                       aGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                                            >  1:3418406/1‑151 (MQ=14)

                                                       aGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                                            <  1:4966541/151‑1 (MQ=9)

                                                       aGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCAGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGATGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                                            <  1:578378/151‑1 (MQ=9)

                                                       aGCGGTGTGGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTg                                                                                            <  2:3181169/151‑1 (MQ=9)

                                                         cGGTGTTGATGGTGATACCGCGCGCTTTTTCTGCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGcc                                                                                          <  2:4815662/151‑1 (MQ=12)

                                                           gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                                                        >  1:3534735/1‑151 (MQ=9)

                                                           gtgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGa                                                                                        <  2:640282/151‑1 (MQ=12)

                                                            tgtTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGAc                                                                                       <  2:2520413/151‑1 (MQ=12)

                                                               tGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGtt                                                                                    <  2:1993373/151‑1 (MQ=12)

                                                                 aTGGTGATACCGCGCGCTTTTTCTTTTTTTGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTaa                                                                                  >  2:407870/1‑151 (MQ=11)

                                                                   ggTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGACGGTGCCGACGTTAACg                                                                                >  1:2066242/1‑151 (MQ=9)

                                                                      gATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTgg                                                                             <  2:1576263/151‑1 (MQ=17)

                                                                       aTACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTggg                                                                            <  1:1164618/151‑1 (MQ=12)

                                                                        tACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTgggg                                                                           >  1:2354098/1‑151 (MQ=9)

                                                                         aCCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGggga                                                                          >  2:5129832/1‑151 (MQ=9)

                                                                            gcgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagg                                                                       <  1:4656645/151‑1 (MQ=12)

                                                                             cgcgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaaggg                                                                      >  1:3222728/1‑151 (MQ=12)

                                                                               cgcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGgggaagggaa                                                                    <  2:3914880/151‑1 (MQ=12)

                                                                                gcTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAAc                                                                   >  1:1467977/1‑151 (MQ=12)

                                                                                      tcttcCGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCaa                                                             <  1:205086/151‑1 (MQ=17)

                                                                                          ccGGAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAAttt                                                         >  1:2510623/1‑151 (MQ=19)

                                                                                             gAGCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTc                                                      <  1:3148107/151‑1 (MQ=25)

                                                                                             gAGCCGAGTCGATCTTGTCGAACTCAACGACCGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTc                                                      <  2:4107871/151‑1 (MQ=255)

                                                                                              aGCCGAGTCGATCTTGGCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCt                                                     <  2:4518565/151‑1 (MQ=255)

                                                                                               gCCGAGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCtt                                                    >  1:3983747/1‑151 (MQ=255)

                                                                                                  gAGTCGAACTTGGCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGGCGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTGCTTTAg                                                 <  2:3337877/151‑1 (MQ=11)

                                                                                                   aGTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGc                                                >  2:2500578/1‑151 (MQ=255)

                                                                                                    gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                                               <  2:3422434/151‑1 (MQ=255)

                                                                                                    gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                                               >  2:4960399/1‑151 (MQ=255)

                                                                                                    gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                                               >  1:808755/1‑151 (MQ=255)

                                                                                                    gTCGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGcc                                               <  1:3049262/151‑1 (MQ=255)

                                                                                                      cGATCTTGTCGAACTCAACGACTGCCGGACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCAc                                             <  2:1815795/151‑1 (MQ=255)

                                                                                                      cGATCTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCAc                                             <  2:4145195/151‑1 (MQ=255)

                                                                                                          cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa                                         <  1:5160120/151‑1 (MQ=255)

                                                                                                          cTTGTCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACa                                         <  1:1339756/151‑1 (MQ=255)

                                                                                                            agTCGAACTCAACGACTGACCAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTAGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGt                                       <  2:1192243/150‑1 (MQ=14)

                                                                                                              tCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGa                                     >  1:4597016/1‑151 (MQ=255)

                                                                                                              tCGAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGa                                     <  1:1767668/151‑1 (MQ=255)

                                                                                                                gAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAAc                                   <  2:2216438/151‑1 (MQ=255)

                                                                                                                gAACTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAAc                                   >  1:1030666/1‑151 (MQ=255)

                                                                                                                  aCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACct                                 <  1:1283676/151‑1 (MQ=255)

                                                                                                                  aCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACct                                 >  1:3917937/1‑151 (MQ=255)

                                                                                                                  aCTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACATGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACct                                 >  2:281491/1‑151 (MQ=255)

                                                                                                                   cTCAACGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACctc                                <  2:1924172/151‑1 (MQ=255)

                                                                                                                      aaCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTg                             >  1:4898233/1‑151 (MQ=255)

                                                                                                                      aaCGACTGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTg                             >  1:3629108/1‑151 (MQ=255)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGCAGGGAACGATCAAATTTTTCTTTCGCCCCGACAGTGCACCCCTTGccca                         >  2:2437522/3‑149 (MQ=14)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTa                         >  2:1554357/3‑151 (MQ=255)

                                                                                                                          actGCCGAACCGAAAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTa                         >  1:4118534/3‑151 (MQ=255)

                                                                                                                                gaaccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAgggg                   >  2:770978/1‑151 (MQ=255)

                                                                                                                                  accgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGAt                 <  1:4389984/151‑1 (MQ=255)

                                                                                                                                   ccgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGAtt                >  1:3959257/1‑151 (MQ=255)

                                                                                                                                    cgaaAACTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTa               <  1:284986/151‑1 (MQ=255)

                                                                                                                                       aaaCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGcc            <  2:3642800/151‑1 (MQ=255)

                                                                                                                                        aaCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCt           >  1:2342833/1‑151 (MQ=255)

                                                                                                                                         aCTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCtt          <  2:1278856/151‑1 (MQ=255)

                                                                                                                                          cTTCGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTg         >  1:1675659/1‑151 (MQ=255)

                                                                                                                                             cGGAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGttt      <  1:3462733/151‑1 (MQ=255)

                                                                                                                                              ggAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGtttt     <  1:897822/151‑1 (MQ=255)

                                                                                                                                               gggCAGACGCGAGTGCGGGCTGCGGTCAGAGTGGTCTTGCCGTGGTCAACGTGGCCCATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACCGTGAACCCCTTGCCTAAAGGGGATTAGCCTTGttttt    <  2:4559467/149‑1 (MQ=18)

                                                                                                                                               gAGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGttttt    >  1:4033163/1‑151 (MQ=255)

                                                                                                                                                aGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGtttttt   <  1:265476/151‑1 (MQ=255)

                                                                                                                                                aGCAGACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGtttttt   <  1:1983132/151‑1 (MQ=255)

GACCTGGGCAGTCAACGTGAGCGTAGTGACGAATGTTCGAGTTGTACTCGACGTGAGCGGTGTTGATGGTGATACCGCGCGCTTTTTCTTCCGGAGCCGAGTCGATCTTGTCGAACTCAACGATTGCCGAACCGAAAACTTCGGAGCAAACGCGAGTCAGAGCTGCGGTCAGAGTGGTCTTACCGTGGTCAACGTGGCCGATGGTGCCGACGTTAACGTGGGGAAGGGAACGATCAAATTTTTCTTTAGCCACGACAGTGAACCTCTTGCCTAAAGGGGATTAGCCTTGTTTTTTA  >  CP009974/1555154‑1555449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`