Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,897,927	A→G	intergenic (‑5705/‑2892)	RPPX_08470 ← / → RPPX_08480	tryptophan synthase subunit beta/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,897,927	0	A	G	100.0%	16.5 / NA	19	intergenic (‑5705/‑2892)	RPPX_08470/RPPX_08480	tryptophan synthase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/4);  total (15/4)

GTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGTGGTGCTGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGGGTG  >  CP009974/1897799‑1897969                                                                                                                                 |                                           gTCTACCAGGACGCTGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGAccc                      >  1:1504616/1‑151 (MQ=1) gTCTACCAGGACGCTGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGAccc                      >  2:4787561/1‑151 (MQ=1) gTCTACCAGGACGCTGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGAccc                      >  1:3254209/1‑151 (MQ=11)             gctggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCg          >  1:1105801/4‑151 (MQ=1)             gctggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCg          >  1:84166/4‑151 (MQ=1)               tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa        >  2:4821959/2‑151 (MQ=1)               tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa        <  2:3015906/150‑1 (MQ=11)               tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa        >  2:2622201/2‑151 (MQ=1)               tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa        >  1:2300553/2‑151 (MQ=1)                  ccAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggc     <  1:5033247/151‑2 (MQ=11)                  ccAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggc     <  1:804753/151‑2 (MQ=11)                   cAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcg    >  2:2407984/1‑149 (MQ=11)                   cAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcg    >  1:1929698/1‑149 (MQ=11)                   cAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGGGCTGCTTCGGTTCCAGTGACCGACACCCTCGACGAAGTGCTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcg    >  1:5222556/1‑149 (MQ=11)                    aGATGAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg   >  2:4478311/1‑148 (MQ=11)                    aGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg   <  1:3968546/151‑4 (MQ=11)                    aGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg   >  1:2661301/1‑148 (MQ=11)                    aGATAAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg   >  2:4478527/1‑148 (MQ=11)                     gATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggg  >  1:2926223/1‑147 (MQ=11)                                                                                                                                 |                                           GTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGTGGTGCTGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGGGTG  >  CP009974/1897799‑1897969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑