Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,915,245 A→G noncoding (50/116 nt) RPPX_08540 ← 5S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,915,2450AG92.5% 399.8 / 14.2 161noncoding (50/116 nt)RPPX_085405S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (4/8);  new base G (80/68);  total (85/76)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 170 total aligned reads displayed.

TCAGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTTGCCAGAATGTCCAGATGGACAGCCCAGCGAATCCGGATATGTGATGTTTGCGAAGCTCTTGTGTAAAGCACGAATTTTCGGGTCTTTC  >  CP009974/1915095‑1915392
                                                                                                                                                      |                                                                                                                                                   
 cAGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCactgct                                                                                                                                                   <  1:4572208/151‑1 (MQ=21)
  cgCTATCAGTTCTAACCGATATTGAGGCCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCActgctg                                                                                                                                                  <  2:4661931/150‑1 (MQ=18)
  aGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCActgctg                                                                                                                                                  <  2:2160358/151‑1 (MQ=21)
     tATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGt                                                                                                                                               <  1:2685087/151‑1 (MQ=21)
      aTCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGtt                                                                                                                                              <  2:431157/151‑1 (MQ=21)
       tCAGTTCTAACTGTTATTGAAGCCCTTGAAAGACAACGCCCCTGTCTGTAACGAAGTCGGGGGCTTTG‑GCTTTGAATCTTGACGATGGCCTACTCTCACCTGGGGAAACCCCACACTAACATCGGCGGTGCATCGTTTCACTACTGAGTTc                                                                                                                                             <  2:4316598/151‑1 (MQ=12)
       tCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTc                                                                                                                                             <  1:3931272/151‑1 (MQ=21)
        cAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCg                                                                                                                                            <  2:1315719/151‑1 (MQ=21)
          gTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCggg                                                                                                                                          <  2:5274860/151‑1 (MQ=18)
           ttCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCggga                                                                                                                                         <  2:4912311/151‑1 (MQ=18)
           ttCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCggga                                                                                                                                         <  1:1788530/151‑1 (MQ=18)
            tCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGCTcacgat                                                                                                                                        >  1:600377/1‑146 (MQ=18)
              tAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggatgg                                                                                                                                      <  2:3156541/151‑1 (MQ=18)
               aaCCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggatggg                                                                                                                                     >  1:2360072/1‑151 (MQ=18)
                aCCGAGATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggatggga                                                                                                                                    <  2:5374502/151‑1 (MQ=18)
                   gATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCa                                                                                                                                 >  2:168864/1‑151 (MQ=18)
                     tatTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAgg                                                                                                                               >  2:3865966/1‑151 (MQ=17)
                      atTGAACCCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAggt                                                                                                                              <  1:4784086/151‑1 (MQ=17)
                      atTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAggt                                                                                                                              >  1:1549153/1‑151 (MQ=17)
                       ttGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAggtg                                                                                                                             <  2:4110702/151‑1 (MQ=17)
                        tGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAggtgg                                                                                                                            >  2:3018975/1‑151 (MQ=17)
                         gAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAggtggt                                                                                                                           <  1:4870486/151‑1 (MQ=17)
                             cctgTGAAAGACAACGCCCCTGTCTGCATCGAAGAAAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCa                                                                                                                       <  2:2349299/148‑1 (MQ=14)
                             cccGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCa                                                                                                                       <  1:3406273/151‑1 (MQ=17)
                               cGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAAt                                                                                                                     >  2:2722421/1‑151 (MQ=14)
                                gtaaaaGACAAACCCTCTCTCTGCATCGCAGACAGGGG‑CTTGGGGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATg                                                                                                                    <  2:2039831/148‑1 (MQ=11)
                                gTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATg                                                                                                                    <  2:1248500/151‑1 (MQ=14)
                                   aaaGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGctc                                                                                                                 <  1:2357614/151‑1 (MQ=14)
                                       aCAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATg                                                                                                             <  1:4597720/151‑1 (MQ=14)
                                         aaaGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGgt                                                                                                           >  1:5252633/1‑151 (MQ=14)
                                         aaaGCCACTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGgt                                                                                                           <  2:866400/151‑1 (MQ=14)
                                          aaGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGgtc                                                                                                          <  2:2491683/151‑1 (MQ=14)
                                             ccccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGctc                                                                                                                 <  2:1563405/141‑1 (MQ=14)
                                             ccccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGctc                                                                                                                 >  1:1563405/1‑141 (MQ=14)
                                              cccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCa                                                                                                      <  1:669925/151‑1 (MQ=14)
                                              cccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCa                                                                                                      >  2:5308831/1‑151 (MQ=14)
                                              cccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCa                                                                                                      >  2:3691203/1‑151 (MQ=14)
                                               ccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCaa                                                                                                     <  2:1119395/151‑1 (MQ=11)
                                               ccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCaa                                                                                                     >  2:4710296/1‑151 (MQ=11)
                                               ccTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCaa                                                                                                     >  1:2819534/1‑151 (MQ=11)
                                                cTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAg                                                                                                    >  1:2914500/1‑151 (MQ=11)
                                                cTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAg                                                                                                    <  1:2225694/151‑1 (MQ=11)
                                                 tGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGa                                                                                                   >  2:5183072/1‑151 (MQ=11)
                                                  gTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGaa                                                                                                  <  2:2526173/151‑1 (MQ=11)
                                                   tCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGaaa                                                                                                 >  1:3601071/1‑151 (MQ=11)
                                                       cATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCt                                                                                             <  1:471232/151‑1 (MQ=11)
                                                        aTCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCACGAAATTccg                                                                                            >  2:5272107/1‑149 (MQ=11)
                                                        aTCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTg                                                                                            <  2:1041130/151‑1 (MQ=11)
                                                         tCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGt                                                                                           <  1:3337618/151‑1 (MQ=11)
                                                          cGCAGACAGGGGCTTTGGGTT‑TGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTg                                                                                          >  1:713011‑M2/1‑150 (MQ=255)
                                                          cGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGgtc                                                                                                          <  2:3263373/135‑1 (MQ=11)
                                                          cGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAggtgg                                                                                                                            <  2:3156773/117‑1 (MQ=11)
                                                                            ggtttgaatCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactag                                                                         >  1:2439011‑M2/5‑133 (MQ=255)
                                                                             gttggaatcTTGACGGTGGCCGACTGTGACATGGGGAAGCCGCACACTACCATCGGGGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactagg                                                                        >  1:4283164‑M2/5‑132 (MQ=255)
                                                                              tttgaATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactagga                                                                       <  1:2116718‑M2/149‑21 (MQ=255)
                                                                              tttgaATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactagga                                                                       <  2:2295085‑M2/149‑21 (MQ=255)
                                                                              tttgaATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactagga                                                                       <  2:2295020‑M2/149‑21 (MQ=255)
                                                                                tGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacg                                                                     >  1:5150334‑M2/1‑129 (MQ=255)
                                                                                     cTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcct                                                                >  2:4982099‑M2/1‑124 (MQ=255)
                                                                                        gACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACGGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcg                                                             <  1:1760273‑M2/151‑31 (MQ=255)
                                                                                         aCGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcga                                                            >  1:1962561‑M2/1‑120 (MQ=255)
                                                                                              gACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctc                                                       <  1:1595287‑M2/151‑37 (MQ=255)
                                                                                              gACCTACTCGCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctc                                                       >  2:3370897‑M2/1‑115 (MQ=255)
                                                                                                     tctcACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagtt                                                >  1:441697‑M2/1‑108 (MQ=255)
                                                                                                     tctcACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagtt                                                >  1:1722547‑M2/1‑108 (MQ=255)
                                                                                                       tcACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcc                                              >  2:2759586‑M2/1‑106 (MQ=255)
                                                                                                        gacatGGGGAATCCCGGCACGACCAGCGGCGATGCATCGCTTCAATGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGGCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatttctgtagttcct                                             >  1:1075449‑M2/2‑105 (MQ=255)
                                                                                                         acaTGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttccta                                            >  1:205626‑M2/1‑104 (MQ=255)
                                                                                                         acaTGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGACCAGGTGGCTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctgccccgccactaggacgcgcctgcgaatctctgtagctccta                                            <  2:2322421‑M2/151‑48 (MQ=255)
                                                                                                          caTGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTttgctggctcgtcactaggacgcgcctgcgaatctctgtagttcctac                                           <  2:1236629‑M2/151‑49 (MQ=255)
                                                                                                             ggggAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctactta                                        >  1:3838701‑M2/1‑100 (MQ=255)
                                                                                                                gAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaag                                     >  1:1929907‑M2/1‑97 (MQ=255)
                                                                                                                gAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaag                                     >  1:1453969‑M2/1‑97 (MQ=255)
                                                                                                                  aaCCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagac                                   <  1:1230562‑M2/151‑57 (MQ=255)
                                                                                                                      ccACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaa                               <  1:4925312‑M2/151‑61 (MQ=255)
                                                                                                                        actcacccatcggcGATGCTGAGTTTAACTGCTGAGTTCGGGATGGTATCGGGTGGTTACAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgacactaggtcgcgccggcgaatctctgtagttcctacttaaagacaaaacc                             >  2:1183238‑M2/7‑89 (MQ=255)
                                                                                                                          acTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccc                           >  1:1366512‑M2/1‑87 (MQ=255)
                                                                                                                          acTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccc                           >  1:3510461‑M2/1‑87 (MQ=255)
                                                                                                                          acTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccc                           >  2:1374406‑M2/1‑87 (MQ=255)
                                                                                                                            tACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaaccccta                         >  1:168864‑M2/1‑85 (MQ=255)
                                                                                                                                  cGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgca                   >  1:319319‑M2/1‑79 (MQ=255)
                                                                                                                                  cGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgca                   >  2:2702652‑M2/1‑79 (MQ=255)
                                                                                                                                   ggCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcat                  >  1:4383639‑M2/1‑78 (MQ=255)
                                                                                                                                   ggCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcat                  <  1:3010865‑M2/151‑74 (MQ=255)
                                                                                                                                   ggCGATGCATCGTTTCACTGCGGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcat                  >  2:3945235‑M2/1‑78 (MQ=255)
                                                                                                                                    gCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcata                 <  1:1615688‑M2/151‑75 (MQ=255)
                                                                                                                                     cGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatac                >  2:2134028‑M2/1‑76 (MQ=255)
                                                                                                                                     cGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatac                >  1:3253007‑M2/1‑76 (MQ=255)
                                                                                                                                       aTGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgc              <  2:745019‑M2/151‑78 (MQ=255)
                                                                                                                                       aTGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgc              >  1:2143455‑M2/1‑74 (MQ=255)
                                                                                                                                          cATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcaga           >  2:2758211‑M2/1‑71 (MQ=255)
                                                                                                                                             cGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatag        >  1:5091803‑M2/1‑68 (MQ=255)
                                                                                                                                             cGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatag        <  2:3197431‑M2/151‑84 (MQ=255)
                                                                                                                                             cGTGTCACTGCTGAGTTCGGGATGGGATCAGGGGGTGCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatag        >  2:5401234‑M2/1‑68 (MQ=255)
                                                                                                                                                ttCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatagggg     >  1:1119840‑M2/1‑65 (MQ=255)
                                                                                                                                                ttCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatagggg     >  1:3056862‑M2/1‑65 (MQ=255)
                                                                                                                                                ttCACTGCTGAGTTCGGGATGGGAGCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaaccccttcctgcatacggagatagggg     >  2:3437110‑M2/1‑65 (MQ=255)
                                                                                                                                                 tCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggt    >  1:895396‑M2/1‑64 (MQ=255)
                                                                                                                                                   actgctGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggttt  <  1:4056002‑M2/151‑90 (MQ=255)
                                                                                                                                                   actgctGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggttt  >  1:3846063‑M2/1‑62 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TCAGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTTGCCAGAATGTCCAGATGGACAGCCCAGCGAATCCGGATATGTGATGTTTGCGAAGCTCTTGTGTAAAGCACGAATTTTCGGGTCTTTC  >  CP009974/1915095‑1915392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑