Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,738,441 A→T V218V (GTA→GTT)  RPPX_12165 → benzene 1,2‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,738,4410AT95.3% 16.3 / ‑1.6 43V218V (GTA→GTT) RPPX_12165benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (25/16);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTG  >  CP009974/2738296‑2738582
                                                                                                                                                 |                                                                                                                                             
aGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGACCAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTc                                                                                                                                          <  2:5382427/151‑1 (MQ=1)
                     agctcatcGACATGAGCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGACAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGc                                                                                                                     <  2:3817212/148‑1 (MQ=0)
                                         gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc                                                                                                 <  1:950757/148‑1 (MQ=0)
                                         gaccAGTCGCCGGACGGTCTGGAAGTGCTGCGTGGAGCTAGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAagc                                                                                                 >  2:3350667/4‑151 (MQ=1)
                                                                         tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGACCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                 <  1:1420902/144‑1 (MQ=1)
                                                                         tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                 <  1:2678076/144‑1 (MQ=1)
                                                                         tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                 <  1:635532/144‑1 (MQ=1)
                                                                           gagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAg                                                               >  1:423224/6‑151 (MQ=1)
                                                                            agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc                                                              >  2:4290040/5‑150 (MQ=1)
                                                                             gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                             >  1:3967119/4‑149 (MQ=1)
                                                                             gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                             <  2:4719174/148‑3 (MQ=1)
                                                                                   tcctACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGgct                                                       >  1:4831024/4‑150 (MQ=0)
                                                                                   tcctACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGgcc                                                       >  2:2158023/4‑150 (MQ=1)
                                                                                            accggaGGCAACTGGAAGCTCCCCGCCGAAACCGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGAAACTACGCTGCCACCCAGAACCAGCGAAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCa                                              <  2:1457271/146‑1 (MQ=1)
                                                                                                  ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                        >  2:324255/4‑151 (MQ=0)
                                                                                                  ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                        >  2:4119968/4‑151 (MQ=0)
                                                                                                  ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                        >  2:1779345/4‑151 (MQ=0)
                                                                                                           aaGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGtt                               >  2:2054432/1‑151 (MQ=0)
                                                                                                              ctgaCCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCt                            <  2:2876788/148‑1 (MQ=0)
                                                                                                              ctgaCCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCt                            <  1:387176/148‑1 (MQ=0)
                                                                                                                       gagaaCGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCg                   >  2:2858554/4‑151 (MQ=0)
                                                                                                                            cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt              >  1:2642600/1‑151 (MQ=2)
                                                                                                                            cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt              <  1:4204967/151‑1 (MQ=2)
                                                                                                                             ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg             <  1:3496694/151‑1 (MQ=2)
                                                                                                                              gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg            >  1:5226025/1‑151 (MQ=2)
                                                                                                                              gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg            <  2:3370886/151‑1 (MQ=2)
                                                                                                                              gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg            >  2:4214548/1‑151 (MQ=11)
                                                                                                                                gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac          >  2:3806900/1‑151 (MQ=2)
                                                                                                                                gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac          <  1:5318288/151‑1 (MQ=2)
                                                                                                                                gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac          >  1:5016622/1‑151 (MQ=2)
                                                                                                                                 ccGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGaca         <  1:743505/151‑1 (MQ=2)
                                                                                                                                    aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct      <  2:4236934/151‑1 (MQ=11)
                                                                                                                                    aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct      >  2:668703/1‑151 (MQ=2)
                                                                                                                                    aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct      >  2:2646067/1‑151 (MQ=2)
                                                                                                                                    aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct      >  2:2644653/1‑151 (MQ=2)
                                                                                                                                    aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct      >  1:2596495/1‑151 (MQ=2)
                                                                                                                                    aCGGCTATCATGTTAGCGCGGTGCACTGGAACTACGCCGCTAATCGCAACCAGCGCAAGCAGCGCGAGGCAGGCGACTAAGTTAAATCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTTGTTGTTTCTATTCCTTCGAGCATGGACTCct      <  2:3041600/151‑1 (MQ=2)
                                                                                                                                     cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg     <  2:2365483/151‑1 (MQ=2)
                                                                                                                                     cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg     >  1:2506923/1‑151 (MQ=11)
                                                                                                                                     cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGGTTCTATTCCTTCCACCATGGACACctg     >  2:4326824/1‑151 (MQ=2)
                                                                                                                                      ggCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctgc    >  2:493223/1‑151 (MQ=11)
                                                                                                                                       gCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctgct   >  1:4515264/1‑151 (MQ=11)
                                                                                                                                        cTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTCCTATTCCTTCGACCATGGACACctgctg  >  2:3228592/1‑151 (MQ=11)
                                                                                                                                                 |                                                                                                                                             
AGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGATCAGTCGCCTGAAGGCCTGGAAGTGCTGCGCGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTG  >  CP009974/2738296‑2738582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑