BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	2,738,498	G→A	A237A (GCG→GCA)	RPPX_12165 →	benzene 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	2,738,498	0	G	A	99.2%	220.9 / ‑5.1	118	A237A (GCG→GCA)	RPPX_12165	benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (63/54); total (63/55)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 120 total aligned reads displayed.

CGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCT  >  CP009974/2738369‑2738645

tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGACCTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                                                                                <  1:1420902/144‑1 (MQ=1)

tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                                                                                <  1:635532/144‑1 (MQ=1)

tggagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATg                                                                                                                                <  1:2678076/144‑1 (MQ=1)

  gagctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAg                                                                                                                              >  1:423224/6‑151 (MQ=1)

   agctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgc                                                                                                                             >  2:4290040/5‑150 (MQ=1)

    gctaGCTCCTACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAgcg                                                                                                                            <  2:4719174/148‑3 (MQ=1)

          tcctACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGgct                                                                                                                      >  1:4831024/4‑150 (MQ=0)

          tcctACATCTACGAAGGTAACTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGgcc                                                                                                                      >  2:2158023/4‑150 (MQ=1)

                   accggaGGCAACTGGAAGCTCCCCGCCGAAACCGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGAAACTACGCTGCCACCCAGAACCAGCGAAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCa                                                                                                             <  2:1457271/146‑1 (MQ=1)

                         ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                                                                                       >  2:324255/4‑151 (MQ=0)

                         ggtaaCTGGAAGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAgg                                                                                                       >  2:4119968/4‑151 (MQ=0)

                                  aaGCTGACCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGtt                                                                                              >  2:2054432/1‑151 (MQ=0)

                                     ctgaCCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCt                                                                                           <  2:2876788/148‑1 (MQ=0)

                                     ctgaCCGCCGAGAACGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCt                                                                                           <  1:387176/148‑1 (MQ=0)

                                                   cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt                                                                             <  1:4204967/151‑1 (MQ=2)

                                                   cGGCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCAt                                                                             >  1:2642600/1‑151 (MQ=2)

                                                    ggCGCCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATg                                                                            <  1:3496694/151‑1 (MQ=2)

                                                     gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                           <  2:3370886/151‑1 (MQ=2)

                                                     gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                           >  1:5226025/1‑151 (MQ=2)

                                                     gcgcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATgg                                                                           >  2:4214548/1‑151 (MQ=11)

                                                       gcCGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGac                                                                         >  2:3806900/1‑151 (MQ=2)

                                                        ccGACGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGaca                                                                        <  1:743505/151‑1 (MQ=2)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                     >  2:2644653/1‑151 (MQ=2)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                     >  2:2646067/1‑151 (MQ=2)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                     <  2:4236934/151‑1 (MQ=11)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                     >  2:668703/1‑151 (MQ=2)

                                                           aCGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACct                                                                     >  1:2596495/1‑151 (MQ=2)

                                                           aCGGCTATCATGTTAGCGCGGTGCACTGGAACTACGCCGCTAATCGCAACCAGCGCAAGCAGCGCGAGGCAGGCGACTAAGTTAAATCCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTTGTTGTTTCTATTCCTTCGAGCATGGACTCct                                                                     <  2:3041600/151‑1 (MQ=2)

                                                            cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg                                                                    <  2:2365483/151‑1 (MQ=2)

                                                            cGGCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctg                                                                    >  1:2506923/1‑151 (MQ=11)

                                                             ggCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctgc                                                                   >  2:493223/1‑151 (MQ=11)

                                                              gCTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACctgct                                                                  >  1:4515264/1‑151 (MQ=11)

                                                               cTATCATGTTAGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTCCTATTCCTTCGACCATGGACACctgctg                                                                 >  2:3228592/1‑151 (MQ=11)

                                                                  tcatgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctc                                                              <  1:4858875/144‑1 (MQ=11)

                                                                  tcatgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctc                                                              <  2:4031951/144‑1 (MQ=11)

                                                                  tcatgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctc                                                              <  1:3610142/144‑1 (MQ=2)

                                                                   catgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGctct                                                             <  1:1415458/145‑1 (MQ=2)

                                                                    atgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTg                                                            >  1:220555/6‑151 (MQ=11)

                                                                    atgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTg                                                            >  2:4730553/6‑151 (MQ=11)

                                                                     tgttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTgg                                                           <  1:3256087/147‑1 (MQ=11)

                                                                       ttaGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGAc                                                         <  2:2715610/149‑1 (MQ=11)

                                                                        taGCTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACt                                                        <  2:1772422/150‑1 (MQ=14)

                                                                           cTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTATGCAGGGCGGTGGTTTCTATTCCTTCGACCATGGACACCTCCTGCTCTGGCCTCgg                                                     >  2:3366426/1‑151 (MQ=14)

                                                                           cTCCGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCgg                                                     >  2:1838182/1‑151 (MQ=14)

                                                                             ccGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTg                                                   <  1:2099453/151‑1 (MQ=14)

                                                                             ccGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTg                                                   <  2:1923450/151‑1 (MQ=14)

                                                                              cGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTgg                                                  <  1:104673/151‑1 (MQ=14)

                                                                              cGTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTgg                                                  <  1:105033/151‑1 (MQ=14)

                                                                               gTGCACTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTggg                                                 <  1:809449/151‑1 (MQ=14)

                                                                                   aCTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGaa                                             <  2:5313792/151‑1 (MQ=14)

                                                                                   aCTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGaa                                             >  1:4736720/1‑151 (MQ=14)

                                                                                   aCTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGaa                                             >  1:2164522/1‑151 (MQ=14)

                                                                                    cTGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTTCTCTGGACTCGGTGGGCGAAc                                            >  1:1760739/1‑151 (MQ=17)

                                                                                     tGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcc                                           >  2:321968/1‑151 (MQ=14)

                                                                                     tGGAACTACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAAcc                                           >  2:2817590/1‑151 (MQ=14)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                     <  1:964545/151‑1 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                     <  1:4515714/151‑1 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                     <  2:1714847/151‑1 (MQ=17)

                                                                                           tACGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAgg                                     >  2:3268979/1‑151 (MQ=17)

                                                                                            aCGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAGCCCCGAGGa                                    >  2:1298712/1‑151 (MQ=17)

                                                                                            aCGCCGCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGa                                    >  2:31300/1‑151 (MQ=17)

                                                                                             cgccgcCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGAt                                   <  1:4949585/151‑1 (MQ=17)

                                                                                                cgCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTTCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAggtttgc                                >  1:408337/2‑146 (MQ=17)

                                                                                                cgCCACTCAGAACCAACGCAAGCAGCGCGAGGCAGGCGACGAAAGCAAAACCAGGAGCGCCGGCTCTTAGGCTAAGCAGGGTGGTGGTGTCTATTCCTTCGACCAGGGACGCCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGc                                <  2:3620336/150‑1 (MQ=255)

                                                                                                 gCCACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGcc                               <  1:890926/151‑1 (MQ=17)

                                                                                                  ccACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGccc                              >  1:514803/1‑151 (MQ=17)

                                                                                                  ccACTCAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGccc                              >  1:786268/1‑151 (MQ=17)

                                                                                                    actcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAg                            >  2:1165595/4‑151 (MQ=17)

                                                                                                     ctcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGc                           >  1:1429328/3‑151 (MQ=18)

                                                                                                     ctcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGc                           >  1:3522604/3‑151 (MQ=18)

                                                                                                      tcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCt                          <  2:368254/150‑1 (MQ=18)

                                                                                                      tcAGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCt                          <  2:1887958/150‑1 (MQ=18)

                                                                                                        aGAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTa                        <  1:4031362/151‑1 (MQ=18)

                                                                                                         gAACCAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTAt                       <  2:3773630/151‑1 (MQ=18)

                                                                                                            ccAGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAg                    >  1:4701486/1‑151 (MQ=18)

                                                                                                             cAGCGCCAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGGGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgc                   <  2:3863099/151‑1 (MQ=255)

                                                                                                              aGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcg                  <  2:2401391/151‑1 (MQ=18)

                                                                                                              aGCGCAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAgcg                  <  2:2573089/151‑1 (MQ=18)

                                                                                                                cgcAAGCAGCGCGAGGCAGGCGACGAAATCAACACCAGGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                <  2:2752582/151‑1 (MQ=255)

                                                                                                                cgcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGcc                <  2:4735549/151‑1 (MQ=255)

                                                                                                                 gcAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCg               >  1:4393400/1‑151 (MQ=18)

                                                                                                                  cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt              >  1:4221109/1‑151 (MQ=255)

                                                                                                                  cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt              >  2:4510787/1‑151 (MQ=255)

                                                                                                                  cAAGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGt              >  2:4508740/1‑151 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg             <  1:592820/151‑1 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg             >  1:2552307/1‑151 (MQ=255)

                                                                                                                   aaGCAGCGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTg             >  1:772281/1‑151 (MQ=255)

                                                                                                                    agcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGa            >  2:1731813/1‑151 (MQ=255)

                                                                                                                    agcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGa            >  1:4316570/1‑151 (MQ=255)

                                                                                                                     gcagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAc           <  1:20598/151‑1 (MQ=255)

                                                                                                                      cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc          <  1:4494949/151‑1 (MQ=18)

                                                                                                                      cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGAcc          <  1:5143558/151‑1 (MQ=18)

                                                                                                                      cagcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGCATCGCCCAGCTTATGAGCGCCGTGAcc          >  2:2738838/1‑151 (MQ=255)

                                                                                                                       agcGCGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCa         >  2:5027670/1‑151 (MQ=255)

                                                                                                                        gcgcGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCaa        >  1:5195923/1‑151 (MQ=255)

                                                                                                                        gcgcGAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCaa        >  1:5196064/1‑151 (MQ=255)

                                                                                                                         cgcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAt       >  2:2961693/1‑151 (MQ=18)

                                                                                                                          gcgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAAtt      >  2:1685961/1‑151 (MQ=255)

                                                                                                                           cgAGGCAGGCGACGAAATCAAAACCATGAGCGCCGGCTCTTGGGCTAAGCAGGGTGGTGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTg     >  2:2850142/1‑151 (MQ=255)

                                                                                                                              ggcgggcgACGAAATCAAAACCAGGAGCGCCGGCTCGTGGGCTAAGCAGGGTGGTGGGTTCTAGTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCt  <  2:5143999/151‑1 (MQ=255)

CGGTGCCAGCTCGTACATCTACGAAGGCAACTGGAAGCTCACCGCCGAAAACGGCGCCGACGGCTACCACGTAAGCTCCGTGCACTGGAACTACGCTGCCACCCAGAACCAGCGCAAGCAGCGCGAGGCGGGTGACGAGATCAAGACCATGAGTGCTGGCGCCTGGGCCAAGCAGGGCGGCGGTTTCTATTCCTTCGACCATGGACACCTGCTGCTCTGGACTCGGTGGGCGAACCCCGAGGATCGCCCAGCTTATGAGCGCCGTGACCAATTGGCT  >  CP009974/2738369‑2738645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`