Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,105,383 2 bp→AA coding (468‑469/1359 nt) RPPX_22650 ← benzene 1,2‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,105,3830GA92.6% 219.1 / 12.6 135L157L (CTG→TTG) RPPX_22650benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base A (54/71);  total (59/76)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
*CP0099745,105,3840GA92.6% 219.4 / 13.0 135F156F (TTC→TTT) RPPX_22650benzene 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base A (54/71);  total (59/76)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAG  >  CP009974/5105240‑5105531
                                                                                                                                               ||                                                                                                                                                   
gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                                                               <  2:2447120/151‑1 (MQ=1)
gTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGccc                                                                                                                                               <  2:2449157/151‑1 (MQ=1)
                          ctccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTTCCACCtt                                                                                                                     >  2:1941651/6‑151 (MQ=1)
                           tccacgcAGCACTTCCAGGCGGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTg                                                                                                                    <  2:3053862/147‑1 (MQ=1)
                           tccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTg                                                                                                                    >  1:3908769/5‑151 (MQ=1)
                            ccacgcAGCACTTCCAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTgg                                                                                                                   >  1:3929407/4‑151 (MQ=1)
                                   ggacgtCCGGGCCTCCAGGCGACTGATCGACGATCATGGCGATGACCTTCGCCGCCCCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTAAGGATGAAGAAAAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTc                                                                                                            <  2:2219530/146‑1 (MQ=0)
                                   gcgctgccAGACCGTCCGGCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTc                                                                                                            <  2:3610142/145‑1 (MQ=1)
                                                gtccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCg                                                                                               <  2:3314535/147‑1 (MQ=1)
                                                 tccggCGACTGGTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGGTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGACTCGTGCCACCTTGGTCAGGGCGTGGGAGCCGTCGc                                                                                              >  2:5211982/4‑151 (MQ=1)
                                                          tGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                                                     <  1:3954260/151‑1 (MQ=11)
                                                           gATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCt                                                                                    >  1:2442686/1‑151 (MQ=11)
                                                            aTCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTg                                                                                   <  1:812696/151‑1 (MQ=11)
                                                              cgacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTc                                                                                 >  2:3141291/1‑151 (MQ=11)
                                                               gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                                                                >  1:3550411/1‑151 (MQ=11)
                                                               gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                                                                <  1:3126539/151‑1 (MQ=11)
                                                               gacgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCg                                                                                <  1:641181/151‑1 (MQ=11)
                                                                acgaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCACGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgg                                                                               >  2:5190524/1‑151 (MQ=11)
                                                                  gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgtgg                                                                             >  1:410258/1‑151 (MQ=11)
                                                                  gaTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCgggg                                                                             >  1:5192008/1‑151 (MQ=11)
                                                                   aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                                                            <  2:1869471/151‑1 (MQ=11)
                                                                   aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                                                            >  1:4082978/1‑151 (MQ=11)
                                                                   aTCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGt                                                                            <  1:1099162/151‑1 (MQ=11)
                                                                       tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                                                        <  1:2923602/151‑1 (MQ=11)
                                                                       tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                                                        <  1:4528427/151‑1 (MQ=11)
                                                                       tGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGcc                                                                        <  1:4623695/151‑1 (MQ=11)
                                                                        gTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCg                                                                       <  1:4637503/151‑1 (MQ=11)
                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                                                      <  1:495812/151‑1 (MQ=11)
                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                                                      >  2:1990908/1‑151 (MQ=11)
                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                                                      <  1:2537538/151‑1 (MQ=11)
                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCgg                                                                      >  1:289304/1‑151 (MQ=11)
                                                                         tCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCAgg                                                                      >  1:287132/1‑151 (MQ=11)
                                                                             tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                                                  <  1:2795052/151‑1 (MQ=11)
                                                                             tgatCTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGtt                                                                  >  1:3592006/1‑151 (MQ=11)
                                                                                 cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                                                              <  1:2678590/151‑1 (MQ=11)
                                                                                 cTTCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTg                                                              >  2:2594966/1‑151 (MQ=11)
                                                                                  ttCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTgg                                                             <  1:1785677/151‑1 (MQ=11)
                                                                                   tCGCCGACTCGCCCAGGTGCTCGACCAGTGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTggg                                                            >  1:5424821/1‑151 (MQ=11)
                                                                                   tCGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTggg                                                            >  1:1078796/1‑151 (MQ=11)
                                                                                   tCGCCGACGCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTggg                                                            <  1:240061/151‑1 (MQ=11)
                                                                                    cGCCGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGTGCCGTCGCAGTTGAAGCTGTCCGGGTAGCCGGCGTTGCTgggg                                                           >  2:1514115/1‑151 (MQ=11)
                                                                                       cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                                                        >  1:511626/1‑151 (MQ=11)
                                                                                       cGACTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCt                                                        >  1:2684962/1‑151 (MQ=11)
                                                                                         aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGc                                                                                     >  2:3117428/1‑120 (MQ=11)
                                                                                         aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                                                      >  2:610384/1‑151 (MQ=11)
                                                                                         aCTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCttt                                                      >  1:75877/1‑151 (MQ=11)
                                                                                          cTCGCCCAGGTGCTCGCCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                                                     <  2:483519/151‑1 (MQ=11)
                                                                                          cTCGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTc                                                     >  2:3616139/1‑151 (MQ=11)
                                                                                           ccGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCGCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCa                                                    <  2:5177899/150‑1 (MQ=11)
                                                                                            cGCCCGGGTGCTCGCCCAGCGGCTTCACATCGGCGTTCCGGCTGCCGAACAAAAAGCCTCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                                                   <  1:4809361/151‑1 (MQ=11)
                                                                                            cGCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAc                                                   <  2:367888/151‑1 (MQ=11)
                                                                                             gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc                                                  >  1:5245481/1‑151 (MQ=14)
                                                                                             gCCCAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCAcc                                                  <  1:2846409/151‑1 (MQ=11)
                                                                                              cccAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCt                                                 >  1:1023682/1‑151 (MQ=14)
                                                                                                cAGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTc                                               <  2:4049353/151‑1 (MQ=14)
                                                                                                 aGGTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTca                                              <  2:3955464/151‑1 (MQ=14)
                                                                                                  ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag                                             <  2:2157034/151‑1 (MQ=14)
                                                                                                  ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag                                             >  2:2115500/1‑151 (MQ=14)
                                                                                                  ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcag                                             >  1:4574808/1‑151 (MQ=14)
                                                                                                  ggTGCTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGCTGCTGGGGTCCTTCACCTCcag                                             >  2:5295321/1‑151 (MQ=11)
                                                                                                     gCTCGACCAGCAGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTcagcag                                          <  1:1671327/151‑1 (MQ=14)
                                                                                                      cTCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGt                                         <  2:1078796/151‑1 (MQ=14)
                                                                                                       tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt                                        >  2:4129790/1‑151 (MQ=14)
                                                                                                       tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt                                        <  1:2821682/151‑1 (MQ=14)
                                                                                                       tCGACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGtt                                        >  2:530034/1‑151 (MQ=14)
                                                                                                         gACCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTg                                      >  2:3328693/1‑151 (MQ=14)
                                                                                                          aCCAGCGGCTTCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGc                                     <  1:3215567/151‑1 (MQ=14)
                                                                                                                ggcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgt                               <  1:856896/148‑1 (MQ=14)
                                                                                                                ggcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgt                               <  1:3040126/148‑1 (MQ=14)
                                                                                                                ggcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgt                               >  2:3036942/4‑151 (MQ=14)
                                                                                                                ggcttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGAtgt                               >  2:5071489/4‑151 (MQ=14)
                                                                                                                  cttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgttg                             <  1:4861306/150‑1 (MQ=14)
                                                                                                                   ttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgttgt                            <  2:847305/151‑1 (MQ=14)
                                                                                                                   ttCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCtgttgt                            <  2:1348911/151‑1 (MQ=14)
                                                                                                                    tCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTgttgtt                           >  1:4837988/1‑151 (MQ=14)
                                                                                                                    tCACATCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTgttgtt                           <  2:4653999/151‑1 (MQ=14)
                                                                                                                     cacaTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGttgttg                          <  2:2782115/151‑1 (MQ=14)
                                                                                                                     cacaTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGttgttg                          <  1:1384509/151‑1 (MQ=14)
                                                                                                                      ccaTGGGCGTTCAGGCTGCCGAACAAAGAGCCCGGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGa                         <  2:5388972/150‑1 (MQ=14)
                                                                                                                       caTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGaa                        >  1:5338875/1‑151 (MQ=14)
                                                                                                                        aTCGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAAc                       <  1:2946515/151‑1 (MQ=14)
                                                                                                                          cGGCGTTCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGt                     <  1:4401847/151‑1 (MQ=14)
                                                                                                                            gcgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTcc                   >  1:1972256/4‑151 (MQ=14)
                                                                                                                            gcgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTcc                   <  1:2730738/148‑1 (MQ=14)
                                                                                                                             cgttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCa                  >  1:132092/3‑151 (MQ=14)
                                                                                                                              gttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGATCGTcgtg                 >  2:3337609/2‑148 (MQ=14)
                                                                                                                              gttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAg                 <  1:1926972/150‑1 (MQ=14)
                                                                                                                               ttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGc                >  1:2577512/1‑151 (MQ=14)
                                                                                                                               ttCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGc                <  2:324767/151‑1 (MQ=14)
                                                                                                                                tCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGcc               >  1:3131597/1‑151 (MQ=14)
                                                                                                                                tCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGcc               <  1:4989441/151‑1 (MQ=14)
                                                                                                                                tCAGGCTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGcc               <  1:4170435/151‑1 (MQ=14)
                                                                                                                                  aggcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGt             <  1:2582547/148‑1 (MQ=14)
                                                                                                                                  aggcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGt             <  1:5041301/148‑1 (MQ=14)
                                                                                                                                   ggcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTg            <  2:1288994/149‑1 (MQ=14)
                                                                                                                                    gcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTgg           <  1:3605870/150‑1 (MQ=14)
                                                                                                                                    gcTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTgg           <  1:2422155/150‑1 (MQ=14)
                                                                                                                                     cTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGACTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGa          >  1:5394675/1‑151 (MQ=14)
                                                                                                                                     cTGCCGAACAAAAAGCCCCGGTACGACTCAAAACGTGCCACCCTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGCTCTTTCACCTCCCGCAGTTTGCCACTGTTGTTGAACGTCCAGCCGtcgc          >  2:3116007/1‑148 (MQ=14)
                                                                                                                                             gaGGCAGTCCCGGTACGCCTCAGACCGTGCCGCCCTGGTCGGGTCGGGGGAGCCGCCGCAGTTGCAGCTGTCGGGGTAGCCGGCGTTGCGGGGGTCTTTCAGCTGCCGCAGTTTGCCGCTGTTGTTGAGCGTCCAGCCGTGGAACGTGCAg  <  2:3294490/150‑1 (MQ=14)
                                                                                                                                               ||                                                                                                                                                   
GTTGCCTTCGTAGATGTACGAGCTGGCACCGCGCAGCACTTCCAGGCCTTCAGGCGACTGATCGACGATCATGTCGATGATCTTCGCCGACTCGCCCAGGTGCTCGACCAGTGGTTTCACATCGGCATTCAGACTGCCAAACAGGAAGCCCCGGTACGACTCGAATCGTGCCACCTTGGTCAGGTCGTGGGAGCCGTCGCAGTTGAAGCTGTCGGGGTAGCCGGCGTTGCTGGGGTCTTTCACCTTCAGCAGTTTGCCGCTGTTGTTGAACGTCCAGCCGTGGAACGGGCAG  >  CP009974/5105240‑5105531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑