Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	418,441	Δ1 bp	intergenic (‑20/+631)	RPPX_01715 ← / ← RPPX_01720	hypothetical protein/lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	418,441	0	A	.	100.0%	105.4 / NA	48	intergenic (‑20/+631)	RPPX_01715/RPPX_01720	hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/22);  total (26/22)

GTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGGA  >  CP009974/418296‑418479                                                                                                                                                  |                                        gttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc                                   <  1:1008939/151‑1 (MQ=255) gttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc                                   <  2:869258/151‑1 (MQ=255) gttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc                                   <  2:868157/151‑1 (MQ=255)  ttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa                                  <  1:1284790/151‑1 (MQ=255)  ttgctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa                                  <  1:3307286/151‑1 (MQ=255)    gctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagg                                <  2:1961597/151‑3 (MQ=255)     ctgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCagga                               <  1:4351450/151‑4 (MQ=255)      tgttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCaggat                              >  2:4476846/1‑147 (MQ=14)       gttgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCca‑ggatg                            >  2:3846463/1‑151 (MQ=14)         tgctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATggg                          >  1:12531/1‑151 (MQ=11)          gctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         >  1:3226432/1‑151 (MQ=11)           ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        <  1:974150/151‑1 (MQ=11)           ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        <  1:3091982/151‑1 (MQ=11)           ctgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCCTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGc                        <  1:974281/151‑1 (MQ=11)            tgttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGcc                       <  1:1232981/151‑1 (MQ=11)             gttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt                      >  2:4122204/1‑151 (MQ=11)             gttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt                      >  2:1694866/1‑151 (MQ=11)             gttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt                      >  2:4121850/1‑151 (MQ=14)             gttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt                      <  1:3362122/151‑1 (MQ=11)              ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         <  2:3479408/147‑1 (MQ=11)              ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATgggg                         >  1:3479408/1‑147 (MQ=11)              ttgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTg                     >  2:2151487/1‑151 (MQ=11)               tgctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGc                    >  2:207222/1‑151 (MQ=14)                gctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCa                   <  2:4713668/151‑1 (MQ=11)                 ctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCTTTGCGGAGGGAGGTGCCGGGCATGGATGGCCGTCAAGCGCTG‑GGCCAC‑CGGATGGGGTCTGCAg                  >  2:1547216/1‑151 (MQ=14)                 ctgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAg                  <  1:4584825/151‑1 (MQ=14)                  tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                 >  1:2226263/1‑151 (MQ=14)                  tgttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgc                 <  1:3590834/151‑1 (MQ=14)                   tttgctgttgctgttgctgttgATGTTGATCTTGATCTTGATCTTGATCTTGTTATTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  2:4471433/150‑1 (MQ=11)                   gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                <  1:624366/151‑1 (MQ=14)                   gttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcg                >  1:2745533/1‑151 (MQ=14)                    ttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGCTGGGGCCTGCAgcgc               >  1:1002232/1‑151 (MQ=14)                    ttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcgc               >  2:2475178/1‑151 (MQ=14)                    ttgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAgcgc               >  1:4100886/1‑151 (MQ=14)                     tgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑g             >  2:2746702/1‑151 (MQ=14)                     tgctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑g             >  1:4226094/1‑151 (MQ=14)                       ctgttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggt           >  1:3877911/1‑149 (MQ=14)                         gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         <  1:4423064/151‑5 (MQ=11)                         gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         >  2:244670/1‑147 (MQ=11)                         gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         >  2:3317453/1‑147 (MQ=11)                         gttgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcc         >  2:3317850/1‑147 (MQ=11)                           tgctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctc       >  1:5215087/1‑145 (MQ=11)                            gctgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGCCGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtccccc      >  1:1982073/1‑144 (MQ=11)                            gctgttgctgttgAGCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGCCTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgc‑ggtcctcc      <  1:248539/151‑8 (MQ=11)                              tgttgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGCCGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCg     <  2:2230974/151‑1 (MQ=11)                              tgttgctgttgATCTTGACCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCg     >  1:3064706/1‑151 (MQ=11)                                 tgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGa  >  2:1711976/1‑151 (MQ=1)                                 tgctgttgATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGTAGGCCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGa  <  1:1185579/151‑1 (MQ=1)                                                                                                                                                  |                                        GTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGCTGTTGATCTTGATCTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTACTCCCGGGA  >  CP009974/418296‑418479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑