| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009974 | 4,031,017 | Δ1 bp | intergenic (+159/‑625) | RPPX_17905 → / → RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009974 | 4,031,017 | 0 | A | . | 93.5% | 84.6 / 3.7 | 32 | intergenic (+159/‑625) | RPPX_17905/RPPX_17915 | 23S rRNA methyltransferase/sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base . (14/15); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAG‑TGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGG > CP009974/4030871‑4031167 | gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc > 1:131616/1‑151 (MQ=255)gtTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc > 1:126644/1‑151 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttctgcTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc > 2:3737682/1‑151 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttctgcTTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccc < 1:4167019/151‑1 (MQ=255) tctgcttctgcttctgcttctgcttctgcttctgctTTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCa < 1:4923055/151‑1 (MQ=16) cttgctcttgctcttgctcttgctttggatttTGCTTTTGCTTCTAAGCGCGCGGTAGTTCTGGCGATGCAGATTGCGAGTTCAGGAGGCCGAGCGCAGGGGTTGCGGAGGGAGGGGACGGGCATGGATGCCCGGCAAGCGCTGgggctcg > 1:3419376‑M2/33‑148 (MQ=255) ctgcttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAg < 2:433220/151‑1 (MQ=255) cttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGAt > 1:166489/1‑151 (MQ=255) cttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGAt < 1:3848694/151‑1 (MQ=255) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATg > 2:536949/1‑151 (MQ=16) ttctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATg > 2:1409882/1‑151 (MQ=16) tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg < 1:3076321/151‑1 (MQ=255) tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg < 1:4738945/151‑1 (MQ=255) tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg < 1:3076308/151‑1 (MQ=255) tctgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgg < 2:5182366/151‑1 (MQ=16) ctgcgtctgcttctgctgctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATggg < 1:3809995/151‑1 (MQ=255) tgcttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATgggg > 2:3607263/1‑151 (MQ=19) ttctgcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCt > 1:4402058/1‑151 (MQ=19) ttctgcttctgctgctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCt < 2:3313541/151‑1 (MQ=12) tctgcttctgctgctgcttctgcttTTGCTGCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtg < 2:3563485/151‑2 (MQ=255) gcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcag > 2:1471906/1‑147 (MQ=9) gcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcag > 2:776108/1‑147 (MQ=9) gcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcag > 1:3774768/1‑147 (MQ=9) gcttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcag < 1:3440283/151‑5 (MQ=255) cttctgcttctgcttgtggttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGGGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc < 1:3563508/151‑6 (MQ=11) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGTGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc > 2:2445136/1‑146 (MQ=11) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc > 2:4459029/1‑146 (MQ=9) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc < 2:929555/151‑6 (MQ=0) cttctgcttctgcttctgcttTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCtgcagc > 1:4979674/1‑146 (MQ=255) gCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGccc < 2:247470/151‑1 (MQ=11) cGACGCAGATTGCGACTGCGGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGGGACGGGCAGGGATGCCCGTCAAGCGC‑GGGGCCCCAGGACGGGGCCTGCAGCGCGGTCCGCCCGGGAGCAAGCCCGGAGCCGAGGGGACCCCGgcgcgcc > 2:389343/1‑150 (MQ=255) gggggggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAG‑TGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTa < 2:1102519/147‑1 (MQ=1) ggtaTGGATGCCCGTCAAGCGGTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAG‑TGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGttt < 1:2453876/148‑1 (MQ=1) aGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAG‑TGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAgg < 1:3838757/151‑1 (MQ=31) | GTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGCAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAG‑TGAGGGGACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGG > CP009974/4030871‑4031167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |