Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,631,689	Δ1 bp	intergenic (+251/‑579)	emrB → / → RPPX_20645	multidrug resistance protein B/oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,631,689	0	G	.	100.0%	181.1 / NA	52	intergenic (+251/‑579)	emrB/RPPX_20645	multidrug resistance protein B/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (26/26);  total (26/26)

GCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631543‑4631712                                                                                                                                                   |                        gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCTCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGACGGATGATAGGAGC‑CAGCg                    >  1:1574065/1‑151 (MQ=11) gcgcAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    <  1:4928410/151‑1 (MQ=255) gCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCg                    >  1:4112682/1‑151 (MQ=255)   gcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  1:4411217/149‑1 (MQ=255)   gcaGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  >  2:4317677/3‑151 (MQ=255)   gaaGGGCTTGCGAAGGGAGGTGACGGCCAGGCATGCCCTTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  <  2:4556062/149‑1 (MQ=11)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGCTGATAGGAGC‑CAGCGtt                  >  2:3544610/1‑151 (MQ=255)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  >  1:4970428/1‑151 (MQ=255)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  >  2:1639231/1‑151 (MQ=255)   gTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtt                  >  2:2500414/1‑151 (MQ=255)    tAGGGCTTGCGAGGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttt                 <  2:1757601/151‑1 (MQ=255)     ggggCTGGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:4678667/150‑1 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCTGACAAGCGCTGCGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:3142872/151‑1 (MQ=11)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                >  1:2184408/1‑151 (MQ=255)     aGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCGGAGGCCGCATGGATGGGGCGTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttt                <  2:4120680/151‑1 (MQ=11)      gggCTTGCGAAGGGAGGTGACGGGCATGGCTGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  1:4568275/151‑1 (MQ=255)      gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               >  2:539402/1‑151 (MQ=255)      gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  2:481380/151‑1 (MQ=255)      gggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               >  2:538378/1‑151 (MQ=255)      gggCTGGCGAAGGGGGGTGACGGGCATGGCTGCCCGTCGAGCGCTGGGGCCCCATGGGTGGGGCCTGCAGCGCGTGCTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGttttt               <  2:2324272/151‑1 (MQ=11)       ggCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGtttttt              >  2:1157774/1‑151 (MQ=255)         cTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTgg            >  1:4901051/1‑151 (MQ=255)          ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           >  2:2438007/1‑151 (MQ=255)          ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGt           <  1:2186073/151‑1 (MQ=255)           tGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          >  2:2351874/1‑151 (MQ=255)           tGCGAAGGGAGGGGACGGGAATGGATGCCCGTCAAGAGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGtt          <  2:3281808/151‑1 (MQ=11)             ggAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:935092/150‑1 (MQ=16)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        >  1:1477864/1‑151 (MQ=255)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        >  1:2148513/1‑151 (MQ=255)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:5207916/151‑1 (MQ=16)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        >  1:2800343/1‑151 (MQ=255)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        <  1:487843/151‑1 (MQ=16)             cGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTAc        >  2:3963277/1‑151 (MQ=19)              gatgggAGGTGACGGGCATGGCTGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       <  1:618810/148‑1 (MQ=255)              gaggggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       <  1:4612747/148‑1 (MQ=9)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAGGCGCTGAGGCCCAATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       <  2:890840/151‑1 (MQ=255)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  2:1376425/1‑151 (MQ=16)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:4183813/1‑151 (MQ=16)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:3015779/1‑151 (MQ=16)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       >  1:1418518/1‑151 (MQ=16)              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACt       <  2:765284/151‑1 (MQ=16)               aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      >  1:1263753/1‑151 (MQ=12)               aaGGGAGGTGACGGGCATGGAGGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  1:4947487/151‑1 (MQ=255)               aaGGGAGGGCACGGGCATGGATGCCCGTCAATCGCTGCGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCCGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtt      <  2:4102181/151‑1 (MQ=11)                aGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttt     >  1:3252992/1‑151 (MQ=9)                 gggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtttt    >  1:3716190/1‑151 (MQ=9)                 gggAGGGGGCGGGGATGGATTCGCGTCAAGCGCTGAGTGGCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGGGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACtttt    <  1:4077356/151‑1 (MQ=11)                  ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   <  2:3851867/151‑1 (MQ=9)                  ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   >  1:2453749/1‑151 (MQ=9)                  ggAGGGGCCGGGCATGGATACCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   <  2:2916467/151‑1 (MQ=11)                  ggAGGGGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGACTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACttttt   <  1:4126599/151‑1 (MQ=11)                   gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTg  <  2:3560958/151‑1 (MQ=2)                                                                                                                                                   |                        GCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGGACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTG  >  CP009974/4631543‑4631712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑