Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,208,862 (C)9→8 coding (661/693 nt) RPPX_05305 ← histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,208,8540C.94.0% 866.5 / 40.3 187coding (669/693 nt)RPPX_05305histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/11);  new base . (96/78);  total (96/91)
Fisher's exact test for biased strand distribution p-value = 2.27e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

CGTATCTACACTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAACCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGTCCTCCTGGC  >  CP009974/1208704‑1209002
                                                                                                                                                      |                                                                                                                                                    
cgtATCTACACTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGaacaac                                                                                                                                                      >  1:2654138/1‑151 (MQ=255)
cgtATCTACACTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGaacaac                                                                                                                                                      <  1:3152219/151‑1 (MQ=255)
   aTCTACACTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAAcccc                                                                                                                                                   >  2:3170314/1‑151 (MQ=255)
      tACACTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAAccccccc                                                                                                                                                <  1:2962906/151‑1 (MQ=255)
         acTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAAccccccccaa                                                                                                                                             >  1:596077/1‑149 (MQ=255)
          cTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCCCCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAAccccccccaag                                                                                                                                            <  1:2001119/151‑4 (MQ=255)
           tGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAAccccccccaagc                                                                                                                                           >  1:2848987/1‑147 (MQ=255)
                tCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGcccc                                                                                                                                     <  2:3148035/151‑1 (MQ=255)
                    cccTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCccgccg                                                                                                                                 >  1:3927062/1‑151 (MQ=255)
                      cTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGtt                                                                                                                               <  1:177118/151‑1 (MQ=255)
                       tGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGCTg                                                                                                                              >  2:1153971/1‑151 (MQ=255)
                        gACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTgg                                                                                                                             >  1:4358589/1‑151 (MQ=255)
                           cGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGgcga                                                                                                                          >  1:4174580/1‑151 (MQ=255)
                           cGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGgaga                                                                                                                          >  1:4688096/1‑151 (MQ=255)
                           cGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGgaga                                                                                                                          >  1:243270/1‑151 (MQ=255)
                            ggACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAACCCCCCCCCAAGCGCCCCGCCGTTGgaga                                                                                                                          <  1:2676223/151‑1 (MQ=255)
                            ggACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGAt                                                                                                                         >  2:3316449/1‑151 (MQ=255)
                               cAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGAtctc                                                                                                                      <  1:327022/151‑1 (MQ=255)
                                aaaaGCCCTGCACCCTCAAGATGGAAATGCCCTACATCACCTGGTGAAACCACCCTCCAACAGCAAAAATTTCAAACGTAGATACAGCCGGACCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCACCTCGCCTTTGGATTtctcc                                                                                                                     >  2:655352/1‑150 (MQ=255)
                                aaaaGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCg                                                                                                                     <  1:2046299/151‑1 (MQ=255)
                                    gCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATcc                                                                                                                 >  1:3891988/1‑151 (MQ=255)
                                    gCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATcc                                                                                                                 <  2:4032060/151‑1 (MQ=255)
                                    gCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATcc                                                                                                                 <  2:2528515/151‑1 (MQ=255)
                                    gCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATcc                                                                                                                 <  1:1018113/151‑1 (MQ=255)
                                     cccTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATccg                                                                                                                >  1:1527310/1‑151 (MQ=255)
                                     cccTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATccg                                                                                                                <  2:4839837/151‑1 (MQ=255)
                                     cccTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATccg                                                                                                                <  1:1831495/151‑1 (MQ=255)
                                        tGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATccgccc                                                                                                             >  2:507097/1‑151 (MQ=255)
                                         gCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATccgcccg                                                                                                            >  2:1479185/1‑151 (MQ=255)
                                           aaCCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGACCCCGATccccccgcc                                                                                                          >  2:900016/1‑150 (MQ=255)
                                               tCAAGATAGAAATGCCCTACATCACCTTGGAAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCAcc                                                                                                      <  1:2217826/151‑1 (MQ=255)
                                                 aaGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCAcccc                                                                                                    <  1:958951/151‑1 (MQ=255)
                                                    aTGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGAACCGCCCGCGCACCccgcc                                                                                                 >  2:1722940/1‑151 (MQ=255)
                                                     tGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCccgccg                                                                                                <  1:525423/151‑1 (MQ=255)
                                                      ggAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCccgcccc                                                                                               >  2:4620324/1‑149 (MQ=255)
                                                      ggAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGCTGGAGATCTCGGTCCGCCCGCGCACCCcgccgc                                                                                               >  1:3445611/1‑151 (MQ=255)
                                                            gCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAAC‑ACCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCAt                                                                                         <  1:4578548/151‑1 (MQ=255)
                                                             cccTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCCTTCAtc                                                                                        >  2:4526860/1‑150 (MQ=255)
                                                              ccTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCTCACCCCGCCGCTTTCAtcc                                                                                       >  1:1709051/1‑149 (MQ=255)
                                                                tACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCaa                                                                                     >  1:4486937/1‑151 (MQ=255)
                                                                 aCATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAc                                                                                    <  2:3397948/151‑1 (MQ=255)
                                                                 aCATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAc                                                                                    >  1:1999475/1‑151 (MQ=255)
                                                                 aCATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAc                                                                                    <  2:230338/151‑1 (MQ=255)
                                                                  cATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAcg                                                                                   >  2:4771748/1‑151 (MQ=255)
                                                                   aTCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAcgc                                                                                  >  1:698929/1‑151 (MQ=255)
                                                                   aTCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAcgc                                                                                  <  1:305757/151‑1 (MQ=255)
                                                                    tCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAAcgcg                                                                                 >  2:1751543/1‑151 (MQ=255)
                                                                     cACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACgcgg                                                                                >  2:3547331/1‑151 (MQ=255)
                                                                        cTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGa                                                                             <  2:4626124/151‑1 (MQ=255)
                                                                        cTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGa                                                                             >  2:383171/1‑151 (MQ=255)
                                                                          tGGGAAGTCGCCCTCCAACTGCAAAACTTTCAAACGTAGATTCAGCCGGGCCTGAAGGTAAAAACAACCTGAAC‑ACCCCCCCCCATGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATc                                                                           <  2:1239955/151‑1 (MQ=255)
                                                                           gggAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATcc                                                                          <  2:131578/151‑1 (MQ=255)
                                                                           gggAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATcc                                                                          <  2:39013/151‑1 (MQ=255)
                                                                              aaGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCcgcg                                                                       <  2:2614926/151‑1 (MQ=255)
                                                                              aaGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCcgcg                                                                       >  1:1427650/1‑151 (MQ=255)
                                                                              aaGCCACCAGCCAACTGCAAAAATTTCGAGCGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGCAC‑AGCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCcgcg                                                                       <  2:2897503/151‑1 (MQ=255)
                                                                                  cACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGcgccgc                                                                   <  2:4141236/151‑1 (MQ=255)
                                                                                   ccccTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCa                                                                  <  1:1399024/150‑1 (MQ=255)
                                                                                   aCCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCa                                                                  >  1:4807536/1‑151 (MQ=255)
                                                                                   aCCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGACATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCTGCTCCGCa                                                                  >  2:674220/1‑151 (MQ=255)
                                                                                    cccTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCaa                                                                 >  1:4042212/1‑151 (MQ=255)
                                                                                          aaCTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACa                                                           <  1:5034376/151‑1 (MQ=255)
                                                                                               cAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAAcccc                                                      >  2:3241573/1‑150 (MQ=255)
                                                                                                aaaaaTTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCaa                                                     <  2:2080241/151‑1 (MQ=255)
                                                                                                aaaaaTTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCaa                                                     <  2:1223117/151‑1 (MQ=255)
                                                                                                 aaaaTTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAg                                                    <  1:316773/151‑1 (MQ=255)
                                                                                                     tttCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCg                                                >  1:582276/1‑151 (MQ=255)
                                                                                                      ttCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCGAGGCCgg                                               >  2:3214044/1‑151 (MQ=255)
                                                                                                              tAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCa                                       >  1:5062571/1‑151 (MQ=255)
                                                                                                               aGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCAc                                      <  1:2585885/151‑1 (MQ=255)
                                                                                                                 aTTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCTCGCACCCCGCCTCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGTCCGGGACTGCCACTg                                    >  1:80570/1‑151 (MQ=255)
                                                                                                                     aGCCGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGgtcg                                >  1:878234/1‑151 (MQ=255)
                                                                                                                       ccGGGCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGgtcgag                              >  1:1445247/1‑151 (MQ=255)
                                                                                                                         gggCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGgtcgagtc                            <  2:3742439/151‑1 (MQ=255)
                                                                                                                          ggCCTCAAGGTAAAAACAACCTGAAC‑ACCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGgtcgagtcg                           <  2:258010/151‑1 (MQ=255)
                                                                                                                           gCCTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGgtcgagtcga                          >  2:1231989/1‑151 (MQ=255)
                                                                                                                            ccTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGgtcgagtcgct                         >  1:5137586/1‑149 (MQ=255)
                                                                                                                            ccTAAAGGTAAAAACAACCTGAAC‑ACCCCCCCCCACGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGAt                         <  2:2710609/151‑1 (MQ=255)
                                                                                                                             cTCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATg                        >  2:922500/1‑151 (MQ=255)
                                                                                                                              tCAAGGTAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACACAAGGCCGGTACTGCCACGGGTCGAGTCGATGc                       >  1:4433704/1‑151 (MQ=255)
                                                                                                                               cAAGGTAAAAACAACCTGAACAA‑CCCCCCCCGAGCGCCCCGCCGGTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGcc                      <  2:79496/151‑1 (MQ=255)
                                                                                                                                 aGGTAAAAACAACCTGAAC‑ACCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCt                    <  1:3770422/151‑1 (MQ=255)
                                                                                                                                    tAAAAACCACCTG‑ACCACCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGa                 <  2:1393343/151‑1 (MQ=255)
                                                                                                                                    tAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGa                 <  1:1239413/151‑1 (MQ=255)
                                                                                                                                    tAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGa                 >  1:3647894/1‑151 (MQ=255)
                                                                                                                                    tAAAAACAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCCGCCATCCGCGCCGCCACATACAACCCAACGCCGGTACTGCCACTCGTCGCGTCGATGCGCTggc                 >  2:5003515/1‑150 (MQ=255)
                                                                                                                                     aaaaaCAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGAt                <  2:107633/151‑1 (MQ=255)
                                                                                                                                      aaaaCAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATg               <  2:243270/151‑1 (MQ=255)
                                                                                                                                      aaaaCAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCCGGATg               >  2:4473957/1‑151 (MQ=255)
                                                                                                                                       aaaCAACCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGt              >  1:3400951/1‑151 (MQ=255)
                                                                                                                                         acaacCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAg            <  1:4071050/151‑1 (MQ=255)
                                                                                                                                          cacccTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGt           <  2:765969/148‑1 (MQ=255)
                                                                                                                                          caacCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGt           >  1:998897/1‑151 (MQ=255)
                                                                                                                                           aacCTGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGtc          <  1:1953917/151‑1 (MQ=255)
                                                                                                                                               tGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGtcctcc      >  1:2279987/1‑151 (MQ=255)
                                                                                                                                               tGAACAA‑CCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGtcctcc      <  2:2862011/151‑1 (MQ=255)
                                                                                                                                                 gaacacCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGTcctcct     <  1:3616698/147‑1 (MQ=255)
                                                                                                                                                 aacaa‑cCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGTCCTCCTg    <  1:4996689/151‑1 (MQ=255)
                                                                                                                                                 aacaa‑cCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGTCCTCCTg    <  1:3677783/151‑1 (MQ=255)
                                                                                                                                                    cccccccccccAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGTCCTCCTGgc  <  2:3280119/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CGTATCTACACTGTCATCTGCCCTGACCGGACAAAAGCCCTGCAACCTCAAGATGGAAATGCCCTACATCACCTTGGGAAGCCACCCTCCAACTGCAAAAATTTCAAACGTAGATTCAGCCGGGCCTCAAGGTAAAAACAACCTGAACAACCCCCCCCCAAGCGCCCCGCCGTTGGAGATCTCGATCCGCCCGCGCACCCCGCCGCTTTCATGCAACGCGGCGATCCGCGCCGCAAAATACAACCCAAGGCCGGTACTGCCACTGGTCGAGTCGATGCCCTGGATGTAGTCCTCCTGGC  >  CP009974/1208704‑1209002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑