Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,634,717 (C)6→7 coding (35/501 nt) RPPX_11730 → metal‑dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,634,7111.C95.5% 828.0 / 30.2 242coding (29/501 nt)RPPX_11730metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base . (2/9);  new base C (125/106);  total (127/115)
Fisher's exact test for biased strand distribution p-value = 2.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TTCAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑C‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTGCGCCAACAGTA  >  CP009974/2634561‑2634855
                                                                                                                                                        |                                                                                                                                                  
ttCAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑a                                                                                                                                                     >  1:3563173/1‑151 (MQ=255)
 tCAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c                                                                                                                                                   >  1:1452823/1‑151 (MQ=255)
  cAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑c                                                                                                                                                 >  1:2903263/1‑151 (MQ=255)
  cAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑c                                                                                                                                                 <  2:3477812/151‑1 (MQ=255)
  cAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑c                                                                                                                                                 <  2:321948/151‑1 (MQ=255)
   aGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc                                                                                                                                                >  2:70098/1‑151 (MQ=255)
   aGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc                                                                                                                                                <  1:3523678/151‑1 (MQ=255)
    gggTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑c                                                                                                                                              >  2:504100/1‑151 (MQ=255)
    gggTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑c                                                                                                                                              <  2:1452823/151‑1 (MQ=255)
      gTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑ccc                                                                                                                                            <  2:2938000/151‑1 (MQ=255)
       tttCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑cccc                                                                                                                                           >  2:118434/1‑150 (MQ=255)
         tCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑ccccgc                                                                                                                                         >  2:2201179/1‑148 (MQ=255)
         tCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑ccccgc                                                                                                                                         <  1:4217529/151‑4 (MQ=255)
         tCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑ccccgc                                                                                                                                         >  1:4863713/1‑148 (MQ=255)
          ccTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑c‑cc‑ccccgca                                                                                                                                        >  1:3399700/1‑147 (MQ=255)
              gtggCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTg                                                                                                                                     >  2:890716/1‑151 (MQ=255)
                    tATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATGTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCCGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCacgcg                                                                                                                               >  1:4773919/1‑147 (MQ=255)
                     aTCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGc                                                                                                                              <  1:4131151/151‑1 (MQ=255)
                          aTGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTg                                                                                                                         >  1:4622244/1‑151 (MQ=255)
                          aTGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTGACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTg                                                                                                                         >  1:724646/1‑151 (MQ=255)
                            gACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCg                                                                                                                       >  1:4855381/1‑151 (MQ=255)
                             aCCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCgg                                                                                                                      >  1:269257/1‑151 (MQ=255)
                             aCCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCgg                                                                                                                      >  1:674184/1‑151 (MQ=255)
                                  gACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAgct                                                                                                                 >  2:4636700/1‑149 (MQ=255)
                                  gACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAgat                                                                                                                 >  1:3814673/1‑151 (MQ=255)
                                  gACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAgat                                                                                                                 <  1:1410936/151‑1 (MQ=255)
                                  gACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCAGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAgat                                                                                                                 <  1:2575801/151‑1 (MQ=255)
                                   aCTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAgatg                                                                                                                >  1:3261598/1‑151 (MQ=255)
                                    cTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAgatga                                                                                                               >  1:1868562/1‑151 (MQ=255)
                                     tGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGCCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCACTAGCCGGTGCCGAAgatgat                                                                                                              >  2:4584270/1‑151 (MQ=255)
                                      gATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATc                                                                                                             <  1:4181657/151‑1 (MQ=255)
                                      gATGCTAGCGGCTTCCCGGCAATTTGCTCCTGGCCACGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATc                                                                                                             <  2:4594999/151‑1 (MQ=255)
                                      gATGCTAGCGGCTTCCCGGCAATTCTCTCCTGGCCAGGGCAGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGCACTACGATACTTGCAGACCT‑ACC‑CC‑CCCGCACCACCAAGAGCAGGTGCGCAAgttactc                                                                                                             >  2:4993138/1‑145 (MQ=255)
                                       aTGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCg                                                                                                            <  1:156115/151‑1 (MQ=255)
                                         gCTAGCGGCTCCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGTCGGAGCAGGTCCGGAACATGATCggc                                                                                                          >  1:1209950/1‑149 (MQ=255)
                                            aGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGc                                                                                                       <  1:4414463/151‑1 (MQ=255)
                                             ggggCTTCGCGGCAGTTTTCTCCTGGCGAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCg                                                                                                      <  2:2078497/149‑1 (MQ=255)
                                                   tCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGcc                                                                                                <  2:2523637/151‑1 (MQ=255)
                                                   tCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGcc                                                                                                >  1:542690/1‑151 (MQ=255)
                                                    cccGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCt                                                                                               <  1:2650181/151‑1 (MQ=255)
                                                     ccGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTg                                                                                              >  1:2457723/1‑151 (MQ=255)
                                                         cAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTg                                                                                          >  2:4901497/1‑151 (MQ=255)
                                                          aaTTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGa                                                                                         <  2:4432592/151‑1 (MQ=255)
                                                               tctcCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATAcc                                                                                    >  2:639209/1‑151 (MQ=255)
                                                               tctcCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATAcc                                                                                    >  2:4376235/1‑151 (MQ=255)
                                                                ttcCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCt                                                                                   <  2:574595/150‑1 (MQ=255)
                                                                ctcCTGGCCACGCCTGTGATTGGCTAGTCTCGGCATTCCTTACGCCGCATACAGCGCCATGACCGTACTACGCTACTTGCAAGGCT‑A‑CTCC‑CCCGCACCTGCAGGAACAGGTAGGGCAGCTGATCAAAAGCGACCGCCCGGCTGAATAcca                                                                                   >  2:3023109/1‑150 (MQ=255)
                                                                 tcCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTg                                                                                  >  2:2011367/1‑151 (MQ=255)
                                                                   cTGGGCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCa                                                                                <  2:4144244/151‑1 (MQ=255)
                                                                   cTGGCCGGGGCTGTGATTGGCTAGAATCGGCAGTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCa                                                                                <  2:3565787/151‑1 (MQ=255)
                                                                   cTGGCCCGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCa                                                                                <  2:2767856/151‑1 (MQ=255)
                                                                   cTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCa                                                                                <  1:921971/151‑1 (MQ=255)
                                                                    gggCCAGGGCTGTGCGAGGCTAGAATCGGCGTTCGTTACGCGGAATACAGCGCGATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCAACAGCGACCGCCTGGGTGAATACCTGCAg                                                                               <  1:464140/150‑1 (MQ=255)
                                                                      gCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAgcg                                                                             <  1:3375172/151‑1 (MQ=255)
                                                                      gCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAgcg                                                                             >  2:295409/1‑151 (MQ=255)
                                                                        cAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGcc                                                                           >  2:2422633/1‑151 (MQ=255)
                                                                              tgtgATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTAcc                                                                     >  2:1927950/1‑151 (MQ=255)
                                                                                tgATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCt                                                                   <  1:371169/151‑1 (MQ=255)
                                                                                    tGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGAcc                                                               >  1:2184581/1‑151 (MQ=255)
                                                                                     ggCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGCGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCCGCGCCGTTACCCAGACCg                                                              >  2:1328371/1‑151 (MQ=255)
                                                                                        tAGACTCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCa                                                           >  2:4322306/1‑151 (MQ=255)
                                                                                        tAGACTCGGCATTAGTTACGCCGGATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCAGCAGGAGCAGGAGCGGAAGATGAACGACAGCGACGGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCa                                                           >  2:4322207/1‑151 (MQ=255)
                                                                                        tAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCa                                                           <  1:4941068/151‑1 (MQ=255)
                                                                                          gAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATg                                                         >  1:4524363/1‑151 (MQ=255)
                                                                                           aaTCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑CCC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGa                                                        <  2:5160246/151‑1 (MQ=255)
                                                                                            aTCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGAc                                                       <  1:389103/151‑1 (MQ=255)
                                                                                            aTCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGAc                                                       >  1:998941/1‑151 (MQ=255)
                                                                                                 cATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCa                                                  >  2:1152543/1‑151 (MQ=255)
                                                                                                  aTTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCaa                                                 >  1:1283302/1‑151 (MQ=255)
                                                                                                    tCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCaaaa                                               >  1:4306804/1‑151 (MQ=255)
                                                                                                      gTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACg                                             >  1:347172/1‑151 (MQ=255)
                                                                                                         aCGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACa                                          <  2:2148167/151‑1 (MQ=255)
                                                                                                            ccGTATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAgg                                       >  2:74320/1‑151 (MQ=255)
                                                                                                             cGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGc                                      >  2:4032219/1‑151 (MQ=255)
                                                                                                                 tACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTg                                  <  1:2236920/151‑1 (MQ=255)
                                                                                                                   cAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTa                                >  1:325112/1‑151 (MQ=255)
                                                                                                                    cgcgcCATGACCGTACTACGTTACTTGCAAGCCTCC‑C‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTAt                               <  1:4470431/150‑1 (MQ=255)
                                                                                                                        ccagcaCCGGACTACGTTACTGGCACCCCT‑A‑C‑CCGCCCGCCCCTGCAGGAGCAGGTGTGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTCCCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCt                           <  2:4003463/146‑1 (MQ=255)
                                                                                                                        ccATGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGAAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCt                           <  1:4281989/151‑1 (MQ=255)
                                                                                                                          aTGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTAt                         >  2:446674/1‑151 (MQ=255)
                                                                                                                           tGACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACACGGCGGTGTATGGCTAtt                        >  1:4075273/1‑150 (MQ=255)
                                                                                                                            gACCGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGc                       <  2:603501/151‑1 (MQ=255)
                                                                                                                               cGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCTATGACGTGCAAAACGACATGGCGTTGTATGGCTATGCCCa                    >  2:1536167/1‑151 (MQ=255)
                                                                                                                               cGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCa                    <  2:3399700/151‑1 (MQ=255)
                                                                                                                               cGTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCa                    <  1:4376235/151‑1 (MQ=255)
                                                                                                                                gTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAg                   <  1:381945/151‑1 (MQ=255)
                                                                                                                                gTACTACGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAg                   <  2:1832712/151‑1 (MQ=255)
                                                                                                                                 tactacGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAgg                  <  2:3253162/151‑1 (MQ=255)
                                                                                                                                   ctacGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACCGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGAt                <  1:84519/151‑1 (MQ=255)
                                                                                                                                    tacGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGc                            >  2:160375/1‑138 (MQ=255)
                                                                                                                                    tacGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGc                            <  1:160375/138‑1 (MQ=255)
                                                                                                                                     acGTTGCTTGCAAGCCTCC‑C‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATCCCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCt              <  2:970758/151‑1 (MQ=255)
                                                                                                                                     acGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCAGCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCt              <  2:4804866/151‑1 (MQ=255)
                                                                                                                                      cGTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCACGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTg             >  1:1747130/1‑151 (MQ=255)
                                                                                                                                       gTTACTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTgc            >  1:5154151/1‑151 (MQ=255)
                                                                                                                                          aCTTGCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCACAACGACAAGTCGATGTTTGGCTATGCCCAGGACCTGcccc         >  1:1133884/1‑151 (MQ=255)
                                                                                                                                              gCAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTGCGCCAACa     <  1:1260827/151‑1 (MQ=255)
                                                                                                                                               cAAGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACACGGCGTTGTATGGCTATGCCCAGGATCTGCGCCAACAg    >  1:5161508/1‑151 (MQ=255)
                                                                                                                                                aaGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTGCGCCAACAGt   >  2:4277395/1‑151 (MQ=255)
                                                                                                                                                 aGCCT‑ACC‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTGCGCCAACAGTa  >  1:4037301/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
TTCAGGGTTTCCTGGTGGCTTATCCGATGACCGCGACTGATGCTAGCGGCTTCCCGGCAATTTTCTCCTGGCCAGGGCTGTGATTGGCTAGAATCGGCATTCGTTACGCCGAATACAGCGCCATGACCGTACTACGTTACTTGCAAGCCT‑A‑C‑CC‑CCCGCACCTGCAGGAGCAGGTGCGGAAGATGATCGACAGCGACCGCCTGGGTGAATACCTGCAGCGCCGTTACCCTGACCGCCATGACGTGCAAAACGACAAGGCGTTGTATGGCTATGCCCAGGATCTGCGCCAACAGTA  >  CP009974/2634561‑2634855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑